Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.09 0.26 0.23 0.31 0.24 0.23 0.2 0.15 – 0.18 0.22 0.14 0.2 0.2 0.19 0.22 0.23 0 0.05 0.1 0.11 0.06 0.05
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.1 0.1 0.1 0.22 0.18 0.07 0.08 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.4 0.08 0.29 0.11 0.42 0.51 0.26 0.75 – 0.71 0.52 0.18 0 0 0.1 0.05 0.08 0.05 0.05 0.06 0.06 0.07 0.07
Nucleus 0.53 0.41 0.53 0.46 0.26 0.28 0.34 0.46 0.34 – 0.33 0.34 0.65 0.6 0.56 0.29 0.37 0.29 0.35 0.35 0.34 0.34 0.33 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0.1 0 0 0.06 0.05 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.11 0.09 0.28 0.14 0.1 0.14 0.06 – 0.05 0.05 0 0.05 0 0 0 0.05 0.14 0.11 0.14 0.06 0.06 0.07
Vac/Vac Membrane 0.12 0.26 0 0.07 0.07 0 0 0 0 – 0 0.06 0.06 0.06 0.11 0.33 0.2 0.25 0.13 0.23 0.23 0.33 0.33 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 1 1 0 1 1 – 0 1 0 0 0 0 0 0 0 0 0 1 5 3
Bud 0 1 2 4 2 5 9 5 16 – 17 9 4 0 0 0 0 1 0 3 4 6 20 24
Bud Neck 0 0 20 6 7 10 13 19 6 – 10 12 0 0 0 0 0 0 0 1 2 1 7 13
Bud Site 0 0 0 0 4 5 10 3 15 – 19 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 12 62 41 80 70 91 89 61 – 83 99 54 99 108 4 44 41 2 8 35 27 37 34
Endoplasmic Reticulum 1 0 1 1 1 0 0 0 0 – 0 0 0 1 0 1 12 8 0 0 0 1 3 3
Endosome 0 4 0 1 9 10 4 4 4 – 0 1 21 51 57 2 44 32 5 13 8 3 9 22
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 1 1 1 0 0 1 1 0 0 1 3 2
Mitochondria 5 55 18 53 28 121 199 115 300 – 324 234 68 18 17 2 10 14 3 8 20 14 44 47
Nucleus 9 57 127 84 67 81 135 198 137 – 151 151 251 301 306 6 75 52 23 56 120 85 195 210
Nuclear Periphery 0 2 0 0 0 0 1 0 1 – 1 0 0 0 1 0 0 0 2 2 2 0 3 2
Nucleolus 2 6 0 2 6 5 7 8 7 – 9 2 7 30 26 2 2 7 4 7 8 4 16 17
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 0 1 0 1 0
SpindlePole 1 2 26 17 72 42 40 59 24 – 25 22 6 27 17 0 8 8 9 18 49 15 35 46
Vac/Vac Membrane 2 36 3 13 17 12 8 14 10 – 14 25 22 29 60 7 41 45 9 36 83 82 198 221
Unique Cell Count 17 139 238 182 255 291 393 435 400 456 447 385 500 546 21 203 177 68 163 359 252 601 672
Labelled Cell Count 23 176 261 222 294 362 517 515 582 653 567 434 558 595 24 236 209 68 163 359 252 601 672


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.1 4.7 4.3 5.0 3.8 3.9 4.2 3.4 – 3.2 3.9 4.8 5.0 5.0 5.8 6.4 6.8 4.4 4.4 4.9
Std Deviation (1e-4) 1.2 1.0 0.8 1.0 1.4 0.8 1.0 0.8 0.7 – 0.6 0.8 1.2 1.6 1.1 0.7 1.5 2.1 1.3 1.2 1.7
Intensity Change (Log2) – – – -0.12 0.1 -0.29 -0.25 -0.14 -0.46 – -0.55 -0.26 0.04 0.12 0.09 0.3 0.46 0.55 -0.09 -0.09 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 6.6 7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9153 1.4965 1.1226 0.7951 0.8849 1.4847 -0.9835 -0.3505 -0.6717 -0.2061 -0.2797 -0.6986 -0.6365 -0.4203 -0.6072 -0.1177 -0.6274 -0.5959
Actin 0.0375 0.0017 0.0108 0.0001 0.0023 0.0096 0.0071 0.0005 0.001 0.025 0.0082 0.0008 0.0123 0.0004 0.0111 0.0353 0.0042 0.0046
Bud 0.0004 0.0002 0.0001 0 0.0087 0.0001 0.0003 0.0002 0.0002 0.0004 0.0005 0.0003 0.0008 0.0062 0.0005 0.0032 0.0003 0.0017
Bud Neck 0.0026 0.0037 0.0034 0.0005 0.0083 0.0142 0.001 0.0011 0.0011 0.0016 0.0021 0.0047 0.0025 0.002 0.0018 0.0014 0.0023 0.0068
Bud Periphery 0.0012 0.0002 0.0002 0 0.0048 0.0002 0.0004 0.0001 0.0003 0.0005 0.0006 0.0003 0.0013 0.0011 0.0005 0.0009 0.0004 0.0009
Bud Site 0.0039 0.0033 0.0021 0.0001 0.0006 0.0005 0.0012 0.0083 0.0017 0.0007 0.0022 0.0007 0.0059 0.0037 0.0037 0.0014 0.0013 0.0004
Cell Periphery 0.0004 0.0003 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0005 0.0001 0.0001
Cytoplasm 0.0587 0.066 0.0388 0.0196 0.0645 0.0909 0.079 0.0859 0.0361 0.0345 0.0572 0.069 0.0531 0.0422 0.0361 0.0686 0.0458 0.0536
Cytoplasmic Foci 0.0183 0.0279 0.0149 0.0007 0.0778 0.0105 0.032 0.0061 0.0093 0.0066 0.0291 0.0071 0.023 0.0108 0.0229 0.0237 0.019 0.0074
Eisosomes 0.0004 0.0004 0.0006 0 0.0001 0.0003 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0002 0.0003 0.0001 0.0003
Endoplasmic Reticulum 0.006 0.0055 0.0043 0.0003 0.0027 0.0041 0.0054 0.0027 0.0041 0.0014 0.0032 0.0021 0.0062 0.002 0.0034 0.0072 0.0018 0.0022
Endosome 0.0178 0.0206 0.0096 0.0004 0.0039 0.0072 0.0283 0.0063 0.0257 0.0332 0.0517 0.0071 0.0192 0.0027 0.0105 0.0099 0.0217 0.0093
Golgi 0.0059 0.0022 0.0013 0 0.0006 0.0006 0.0069 0.0013 0.0042 0.0322 0.0319 0.0039 0.003 0.0003 0.0026 0.0042 0.0044 0.0022
Lipid Particles 0.0138 0.0201 0.0119 0 0.0046 0.0038 0.0249 0.0033 0.0077 0.0044 0.0151 0.0049 0.0094 0.0014 0.0124 0.0028 0.0194 0.0071
Mitochondria 0.0025 0.0022 0.0031 0.0003 0.0029 0.0017 0.0161 0.0017 0.0037 0.026 0.0125 0.0047 0.0041 0.0073 0.0036 0.0127 0.0044 0.0032
None 0.153 0.2282 0.1435 0.1391 0.1899 0.2118 0.0655 0.0945 0.0143 0.0443 0.0605 0.0456 0.082 0.0508 0.0598 0.0301 0.0406 0.0415
Nuclear Periphery 0.0371 0.0255 0.0318 0.0046 0.0475 0.02 0.0555 0.0334 0.0597 0.0261 0.04 0.0247 0.0486 0.0255 0.0313 0.012 0.019 0.0158
Nucleolus 0.0198 0.028 0.0303 0.0068 0.0128 0.036 0.0074 0.0161 0.023 0.01 0.0128 0.0208 0.012 0.022 0.0158 0.0401 0.0165 0.0183
Nucleus 0.4295 0.3578 0.465 0.7598 0.4746 0.4508 0.5693 0.614 0.6913 0.5661 0.5933 0.7019 0.5015 0.5724 0.5112 0.5815 0.4982 0.6238
Peroxisomes 0.009 0.0043 0.0118 0 0.0078 0.0024 0.0077 0.003 0.0031 0.0025 0.0129 0.0079 0.0015 0.0006 0.0114 0.0011 0.0032 0.0017
Punctate Nuclear 0.1772 0.198 0.2125 0.0672 0.0832 0.1306 0.0865 0.1181 0.1079 0.1726 0.0587 0.0905 0.2059 0.247 0.2583 0.1597 0.294 0.1972
Vacuole 0.004 0.0033 0.0028 0.0002 0.0012 0.0039 0.0041 0.0026 0.004 0.0061 0.0045 0.0022 0.0053 0.0013 0.0019 0.0024 0.002 0.0014
Vacuole Periphery 0.0009 0.0007 0.0007 0.0001 0.0008 0.0006 0.0014 0.0007 0.0016 0.0056 0.0028 0.0007 0.0016 0.0005 0.0008 0.0012 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.829 6.16 3.9996 7.8933 7.8176 5.1653 8.1748 9.8526 6.6516 10.3037
Translational Efficiency 0.4171 0.486 0.7489 0.3657 0.3124 0.8198 0.4719 0.3144 0.5088 0.3969

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
Localization
Cell Percentages nucleus (77%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Apc2

Apc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available