Standard name
Human Ortholog
Description Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.59 0.51 0.64 0.87 0.94 0.93 0.91 – 0.92 0.87 0.85 0.66 0.65 0.72 0.9 0.81 0.82 0.06 0.07 0 0.07 0.1 0.09
Endoplasmic Reticulum 0.32 0.32 0.36 0.31 0 0 0 0 – 0 0 0 0.28 0.3 0.25 0 0 0 0.53 0.49 0.67 0.59 0.53 0.37
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.07 – 0.11 0.18 0.16 0 0 0 0.08 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.17 0.18 0.11 0.14 0.09 0.07 0.06 – 0 0 0.05 0.09 0.09 0 0.06 0.13 0.13 0.35 0.36 0.21 0.24 0.26 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 5 2 0 0 1 – 1 1 1 8 8 3 1 3 4 0 1 7 1 9 6
Bud 0 2 2 2 1 4 6 10 – 18 26 18 1 1 0 0 0 1 0 1 3 3 11 10
Bud Neck 0 0 0 0 0 0 0 2 – 0 0 2 1 1 5 1 0 0 0 1 1 1 6 4
Bud Site 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 4 1 0 1 2 – 2 0 6 16 16 21 1 3 1 0 0 0 0 1 0
Cytoplasm 23 93 237 270 532 672 943 972 – 979 524 845 392 392 461 65 177 163 5 15 18 24 63 61
Endoplasmic Reticulum 12 50 165 130 23 2 5 2 – 3 2 5 167 181 162 0 8 6 50 104 358 219 347 251
Endosome 0 1 13 5 17 4 4 12 – 6 2 7 1 1 2 0 5 4 0 3 9 3 6 8
Golgi 0 0 0 0 1 0 0 0 – 0 1 0 1 1 1 0 0 0 0 0 2 0 2 1
Mitochondria 1 1 1 1 2 28 53 70 – 119 110 161 0 0 0 6 7 7 2 3 9 18 25 21
Nucleus 1 0 1 0 1 1 5 2 – 1 3 5 2 2 3 1 2 3 0 0 0 0 0 0
Nuclear Periphery 0 1 3 4 0 1 3 7 – 3 2 16 0 0 0 0 0 1 0 2 4 2 3 4
Nucleolus 0 0 0 0 1 1 0 4 – 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 4 – 5 0 9 0 0 0 0 0 1 0 0 0 1 1 3
Vac/Vac Membrane 1 26 85 48 85 63 76 64 – 37 23 51 56 53 27 4 28 25 33 77 113 91 169 293
Unique Cell Count 38 157 464 422 609 714 1014 1066 1063 602 997 593 602 639 72 218 198 95 214 534 373 655 676
Labelled Cell Count 38 175 511 469 666 777 1097 1152 1176 696 1126 645 656 685 79 233 216 95 214 534 373 655 676


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.7 6.7 6.9 6.1 4.4 3.8 4.1 – 3.5 3.8 3.6 8.0 8.0 7.4 5.2 6.3 7.3 9.0 8.5 7.9
Std Deviation (1e-4) 1.2 1.4 1.5 1.7 1.6 0.9 0.9 0.9 – 0.8 1.0 0.8 1.3 1.3 1.4 1.1 1.3 1.4 1.7 1.8 1.5
Intensity Change (Log2) – – – 0.05 -0.13 -0.62 -0.81 -0.71 – -0.95 -0.84 -0.91 0.24 0.24 0.14 -0.37 -0.1 0.11 0.41 0.34 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0.7 0.7 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 3.6 3.5 3.9 0 0 0
Cytoplasm 3.9 13.1 17.2 18.6 17.7 – 18.3 12.9 13.7 4.9 4.6 7.2 6.2 7.5 7.5
Endoplasmic Reticulum -1.5 -13.6 0 0 0 – 0 0 0 -2.6 -1.9 -3.7 -6.1 -8.9 -8.8
Endosome -1.7 0 0 0 0 – 0 0 0 -3.7 -3.7 -3.5 0 -0.4 -0.6
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -1.9 -4.8 -6.2 -7.5 – -9.8 -7.8 -8.1 -4.2 -4.6 -7.7 -2.7 -1.8 -1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0098 0.0004 0.0107 0.0003 0.0304 0.0003 0.0068 0.0013 0.0132 0.0283 0.0001 0.0001
Bud – – – – – – 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0011 0.0004 0.0001 0
Bud Neck – – – – – – 0.0004 0.0002 0.0008 0.0004 0.0023 0.0009 0.0008 0.0002 0.0006 0.0134 0.0007 0.0005
Bud Periphery – – – – – – 0.0008 0.0001 0.0002 0.0003 0.0003 0.0003 0.0002 0.0004 0.0037 0.0006 0.0001 0.0001
Bud Site – – – – – – 0.0016 0.0011 0.0026 0.0006 0.0007 0.0002 0.003 0.0011 0.0043 0.0054 0.0001 0.0001
Cell Periphery – – – – – – 0.0011 0.0009 0.0009 0.0009 0.0009 0.0006 0.0006 0.001 0.0015 0.0007 0.0004 0.0002
Cytoplasm – – – – – – 0.1784 0.3999 0.3028 0.308 0.2953 0.3173 0.2083 0.382 0.2923 0.3024 0.2711 0.3279
Cytoplasmic Foci – – – – – – 0.026 0.0107 0.0148 0.0125 0.0376 0.0109 0.0166 0.0196 0.0162 0.0133 0.0131 0.009
Eisosomes – – – – – – 0.0002 0 0.0001 0 0.0001 0 0.0001 0.0002 0.0002 0.0004 0 0
Endoplasmic Reticulum – – – – – – 0.4457 0.3503 0.4712 0.4282 0.3015 0.4393 0.524 0.3805 0.4317 0.456 0.4565 0.4979
Endosome – – – – – – 0.0701 0.052 0.0572 0.0808 0.0691 0.0641 0.0535 0.0426 0.0589 0.0658 0.0687 0.0385
Golgi – – – – – – 0.0109 0.0009 0.0019 0.0013 0.0185 0.0058 0.0051 0.0008 0.0029 0.0033 0.0009 0.0004
Lipid Particles – – – – – – 0.0243 0.0058 0.0091 0.0057 0.0087 0.0046 0.0175 0.0048 0.009 0.0044 0.0068 0.0018
Mitochondria – – – – – – 0.0172 0.0003 0.0015 0.0006 0.002 0.0015 0.0012 0.0035 0.0041 0.0012 0.0004 0.0003
None – – – – – – 0.1095 0.1196 0.0479 0.0196 0.0575 0.0233 0.0581 0.0945 0.0685 0.0233 0.0685 0.0441
Nuclear Periphery – – – – – – 0.033 0.0186 0.0216 0.0197 0.0463 0.0289 0.048 0.018 0.0293 0.0205 0.0337 0.0317
Nucleolus – – – – – – 0.0009 0.0002 0.0006 0.0002 0.0002 0.0001 0.0009 0.0002 0.0002 0.0004 0.0001 0.0001
Nucleus – – – – – – 0.0057 0.0047 0.0117 0.0041 0.0099 0.0131 0.0168 0.0084 0.0042 0.0032 0.004 0.0086
Peroxisomes – – – – – – 0.0038 0.0001 0.0011 0.0001 0.0157 0.0001 0.0025 0.0033 0.003 0.0023 0.0001 0
Punctate Nuclear – – – – – – 0.0075 0.0012 0.0017 0.0009 0.0012 0.0005 0.0073 0.0012 0.0017 0.0017 0.0006 0.0007
Vacuole – – – – – – 0.049 0.0313 0.0377 0.1092 0.0969 0.0826 0.0256 0.0339 0.0477 0.0513 0.0658 0.0345
Vacuole Periphery – – – – – – 0.0037 0.0016 0.0036 0.0062 0.0046 0.0056 0.0028 0.0021 0.0055 0.0018 0.0082 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.7377 24.8732 19.0337 20.4277 21.9834 26.0708 19.2549 15.9132 15.9209 23.1137
Translational Efficiency 0.4922 0.2627 0.4319 0.3895 0.3867 0.3669 0.3024 0.3303 0.3382 0.3094

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
Localization
Cell Percentages ER (65%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Zrt2

Zrt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zrt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available