Standard name
Human Ortholog
Description Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0.07 0.08 0.07 0.15 0.13 0.12
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.88 0.86 0.8 0.94 0.92 0.86 0.81 0.84 0.78 – 0.8 0.71 0.97 0.99 0.98 0.84 0.8 0.85 0.7 0.64 0.59 0.38 0.43 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.14 0.21 0.13 0.35 – 0.36 0.36 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0.06 0.05 0.14 0 0.05 0.07 0.07 0.07 0.06 – 0 0.05 0 0 0 0 0.09 0 0.06 0.06 0.11 0.05 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.09 0.11 0.08 0.11 0.05
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.09 0.09 0 0 0 0.13 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 2 1 0 0 4 4
Bud 1 3 1 0 1 2 4 4 4 – 0 6 1 0 0 1 2 1 23 16 7 13 27 20
Bud Neck 2 0 0 0 1 1 2 5 5 – 1 1 0 0 0 0 0 0 1 3 0 1 4 2
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 1 0 – 0 0 0 1 0 10 14 5 1 1 0 0 1 1
Cytoplasm 300 176 74 64 59 85 98 162 111 – 47 85 229 266 291 223 140 166 248 139 62 32 95 89
Endoplasmic Reticulum 0 0 0 0 1 0 2 0 0 – 0 0 2 0 2 16 9 6 0 0 0 1 2 0
Endosome 4 6 1 0 0 0 0 0 1 – 0 0 0 0 0 5 2 6 5 3 0 3 5 5
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 2 1 1 0 0 0 0 0
Mitochondria 7 4 4 5 1 14 26 25 49 – 21 43 2 0 2 7 6 3 3 4 3 3 11 4
Nucleus 20 11 13 1 3 7 9 13 8 – 1 6 0 1 0 11 15 7 21 13 11 4 10 5
Nuclear Periphery 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 1 4 3 1 0 2 3 2 2 – 1 0 1 0 0 6 3 2 9 5 2 3 6 4
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 2 0 2 0 0 0 1 2 0 – 0 1 0 0 0 0 1 1 26 20 11 6 23 9
Vac/Vac Membrane 11 10 2 0 0 1 1 1 3 – 0 4 0 2 3 29 16 18 5 3 3 11 17 18
Unique Cell Count 339 205 93 68 64 99 121 194 142 59 119 235 268 296 266 175 196 356 217 106 86 220 173
Labelled Cell Count 348 214 100 71 67 113 147 216 183 71 146 235 270 298 311 211 217 356 217 106 86 220 173


Bud, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.1 4.7 4.3 5.1 3.8 4.0 4.2 3.5 – 3.4 3.5 4.5 4.9 4.8 8.3 8.6 8.4 4.9 4.8 5.3
Std Deviation (1e-4) 0.9 1.1 0.8 0.9 1.2 0.8 1.1 1.1 0.5 – 1.0 0.8 1.0 1.2 1.2 2.4 2.9 2.9 1.4 1.6 2.1
Intensity Change (Log2) – – – -0.13 0.12 -0.3 -0.23 -0.16 -0.44 – -0.5 -0.42 -0.06 0.06 0.03 0.82 0.87 0.83 0.04 0.02 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 2.6 2.2 1.2 0.3 0.8 -0.3 – 0 -1.4 5.5 7.0 6.6 0.9 0.1 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus -2.8 -1.9 -1.6 -1.6 -2.0 -2.2 – -2.6 -2.3 0 0 0 -3.3 -1.4 -3.3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 2.6 2.2 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3567 -0.7194 -0.5876 -0.5172 -0.4657 -0.288 0.5467 -0.0005 0.0718 0.3618 0.287 0.4991 0.4247 -0.3365 -0.2262 0.5064 0.561 0.2619
Actin 0.0128 0.0004 0.0099 0.0032 0.0536 0.0015 0.0233 0.0023 0.0108 0.0024 0.0134 0.0079 0.0154 0.0003 0.0063 0.0004 0.0025 0.0053
Bud 0.0025 0.0073 0.003 0.0003 0.0171 0.0103 0.0095 0.0276 0.0055 0.015 0.0112 0.0158 0.0101 0.0102 0.0101 0.0148 0.0054 0.0178
Bud Neck 0.0106 0.0005 0.0008 0.0003 0.0014 0.0224 0.0047 0.0008 0.0019 0.0049 0.0021 0.0246 0.0038 0.0007 0.0008 0.0021 0.0025 0.0137
Bud Periphery 0.0026 0.0046 0.0036 0.0003 0.0192 0.0113 0.0104 0.0097 0.007 0.0155 0.0098 0.0218 0.0096 0.0049 0.0068 0.0165 0.0105 0.0156
Bud Site 0.0154 0.0133 0.0011 0.0007 0.0067 0.0052 0.0258 0.0381 0.0195 0.0116 0.0077 0.0064 0.0164 0.0217 0.013 0.0016 0.0023 0.0059
Cell Periphery 0.0008 0.0004 0.0002 0.0001 0.0004 0.0005 0.0017 0.0009 0.0006 0.0006 0.0005 0.0005 0.0008 0.0005 0.0005 0.0003 0.0003 0.0005
Cytoplasm 0.1613 0.2042 0.1786 0.5007 0.3142 0.1436 0.3299 0.2288 0.3229 0.4922 0.5348 0.2557 0.2446 0.1917 0.223 0.4634 0.3969 0.1767
Cytoplasmic Foci 0.0208 0.0085 0.0266 0.0235 0.0182 0.0096 0.0252 0.0079 0.0232 0.0221 0.0374 0.0098 0.016 0.0105 0.0064 0.0143 0.0118 0.0066
Eisosomes 0.0004 0.0001 0.0002 0 0.0009 0.0001 0.002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0061 0.0021 0.0037 0.0089 0.0043 0.0019 0.0043 0.0019 0.0029 0.0035 0.0032 0.0019 0.0069 0.0014 0.0019 0.0018 0.0052 0.0018
Endosome 0.0104 0.0019 0.0097 0.0141 0.0465 0.0031 0.0142 0.002 0.009 0.0234 0.0242 0.0037 0.0131 0.0019 0.0023 0.0066 0.0201 0.0034
Golgi 0.0039 0.0002 0.0031 0.0063 0.0195 0.0004 0.0057 0.0003 0.0025 0.001 0.0043 0.0014 0.0031 0.0002 0.0014 0.0003 0.0057 0.0004
Lipid Particles 0.0188 0.0003 0.0068 0.0095 0.0358 0.0017 0.0106 0.0006 0.0066 0.0012 0.0049 0.0005 0.0058 0.0005 0.0005 0.0001 0.0118 0.0004
Mitochondria 0.0016 0.0007 0.001 0.0499 0.0166 0.0011 0.0074 0.0007 0.0105 0.0013 0.0012 0.0015 0.0027 0.0006 0.0008 0.0004 0.0039 0.0015
None 0.6038 0.7192 0.7265 0.3306 0.3398 0.3378 0.3914 0.5997 0.5424 0.3293 0.2893 0.2297 0.5035 0.7009 0.685 0.4266 0.3677 0.3281
Nuclear Periphery 0.0097 0.0011 0.0025 0.0057 0.007 0.0054 0.0053 0.0041 0.0018 0.0048 0.0036 0.0068 0.0152 0.0013 0.0026 0.0014 0.0294 0.0081
Nucleolus 0.0042 0.0033 0.0012 0.0003 0.0016 0.038 0.0081 0.0044 0.0026 0.0056 0.0018 0.0391 0.0066 0.0029 0.0025 0.0049 0.0106 0.0306
Nucleus 0.0938 0.0267 0.0051 0.0033 0.0222 0.3647 0.0938 0.0519 0.0125 0.0269 0.0268 0.3363 0.099 0.0425 0.0253 0.0334 0.0868 0.3403
Peroxisomes 0.006 0.0002 0.0061 0.0363 0.0646 0.0018 0.0101 0.0007 0.0077 0.0022 0.0038 0.0025 0.0046 0.0004 0.0005 0.0004 0.0051 0.0007
Punctate Nuclear 0.0103 0.0018 0.008 0.0012 0.0039 0.0334 0.008 0.0136 0.0047 0.0274 0.0138 0.03 0.0159 0.004 0.0071 0.0051 0.0114 0.038
Vacuole 0.0033 0.0029 0.0021 0.0038 0.0047 0.0054 0.0077 0.0036 0.0043 0.0082 0.0053 0.0035 0.0054 0.0025 0.0026 0.0048 0.0073 0.004
Vacuole Periphery 0.0009 0.0003 0.0004 0.0008 0.002 0.0007 0.0011 0.0004 0.0007 0.001 0.0008 0.0006 0.0013 0.0002 0.0004 0.0006 0.0024 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6786 19.6166 23.1004 19.4148 6.1851 10.966 28.1346 36.3773 21.5711 11.489
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (35%), nucleus (16%), mixed (24%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (0.00033)
nucleus - T/G1 (0.0002)
Subcompartmental Group N/A

Ace2

Ace2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ace2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available