Standard name | |
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Human Ortholog | |
Description | Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 |
Bud | 0 | 0 | 0 | 2 | 0 | 7 | 4 | – | 7 | 11 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 4 | 5 | 7 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 3 | – | 3 | 10 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 2 | – | 4 | 8 | 9 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 30 | 27 | 32 | 75 | 78 | 119 | – | 111 | 105 | 76 | 112 | 55 | 69 | 98 | 83 | 68 | 45 | 4 | 14 | 9 | 10 | 9 | 9 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 23 | 12 | 12 | 0 | 1 | 1 | 0 | 0 | 2 |
Endosome | 1 | 2 | 1 | 7 | 8 | 1 | 9 | – | 3 | 2 | 1 | 2 | 5 | 7 | 17 | 27 | 18 | 16 | 4 | 5 | 5 | 10 | 18 | 32 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 3 | 1 | 1 | 0 | 1 | 4 |
Mitochondria | 30 | 35 | 3 | 21 | 19 | 162 | 97 | – | 313 | 282 | 219 | 347 | 6 | 15 | 2 | 16 | 3 | 9 | 0 | 6 | 4 | 10 | 24 | 14 |
Nucleus | 219 | 170 | 202 | 179 | 121 | 94 | 172 | – | 223 | 220 | 163 | 249 | 206 | 234 | 255 | 58 | 47 | 25 | 214 | 159 | 184 | 105 | 132 | 73 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 1 | 2 | – | 3 | 0 | 0 | 4 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 3 | 4 |
Nucleolus | 2 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 1 | 30 | 29 | 12 | 21 | 31 | 28 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 9 | 5 | 3 | 1 | 22 | – | 4 | 10 | 6 | 9 | 2 | 3 | 3 | 0 | 0 | 4 | 11 | 14 | 15 | 12 | 29 | 27 |
Vac/Vac Membrane | 30 | 39 | 18 | 7 | 43 | 15 | 32 | – | 39 | 38 | 21 | 48 | 23 | 32 | 69 | 63 | 46 | 34 | 43 | 40 | 58 | 76 | 113 | 104 |
Unique Cell Count | 270 | 248 | 249 | 234 | 247 | 274 | 401 | 554 | 529 | 393 | 602 | 287 | 349 | 417 | 226 | 168 | 115 | 324 | 284 | 303 | 263 | 383 | 326 | |
Labelled Cell Count | 302 | 280 | 262 | 254 | 269 | 365 | 462 | 711 | 686 | 505 | 798 | 298 | 363 | 446 | 276 | 197 | 148 | 324 | 284 | 303 | 263 | 383 | 326 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.5 | 5.3 | 4.9 | 5.2 | 3.3 | 4.3 | – | 3.8 | 3.9 | 4.0 | 3.9 | 5.4 | 4.8 | 5.5 | 5.9 | 6.3 | 5.9 | 4.7 | 5.1 | 5.3 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.0 | 1.2 | 1.2 | 0.9 | 1.3 | – | 0.9 | 0.9 | 1.3 | 0.7 | 1.7 | 1.3 | 1.7 | 1.7 | 1.7 | 2.1 | 1.5 | 2.1 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.03 | -0.68 | -0.31 | – | -0.49 | -0.42 | -0.41 | -0.45 | 0.04 | -0.13 | 0.05 | 0.16 | 0.24 | 0.16 | -0.16 | -0.05 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.0 | 5.6 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5925 | -0.4141 | -0.5782 | -1.0401 | -1.0773 | -0.3801 | 2.3193 | 2.4213 | 1.8337 | 2.1338 | 0.6534 | 2.7254 | 3.1502 | 3.5706 | 3.2364 | 2.9912 | 3.2716 | 3.3068 |
Actin | 0.0186 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0029 | 0.0034 | 0.0002 | 0.0218 | 0.009 | 0.0327 | 0.0003 | 0.0269 | 0.0002 | 0.0053 | 0.0001 | 0.0022 | 0.0007 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0015 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0014 | 0.0013 | 0.001 | 0.0006 | 0.001 | 0.0073 | 0.0015 | 0.0016 | 0.0019 | 0.0018 | 0.0024 | 0.0025 | 0.006 | 0.0013 | 0.0025 | 0.0007 | 0.0012 | 0.0052 |
Bud Periphery | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0023 | 0.0033 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0002 |
Bud Site | 0.0006 | 0.0022 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0008 | 0.0026 | 0.0028 | 0.0176 | 0.0007 | 0.0002 | 0.0037 | 0.0013 | 0.002 | 0.0003 | 0.0005 | 0.0004 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0145 | 0.0367 | 0.0218 | 0.0118 | 0.0207 | 0.0173 | 0.1217 | 0.1135 | 0.1056 | 0.1603 | 0.0196 | 0.1059 | 0.0237 | 0.0275 | 0.0169 | 0.028 | 0.0027 | 0.0145 |
Cytoplasmic Foci | 0.009 | 0.0061 | 0.0028 | 0.0002 | 0.0007 | 0.003 | 0.0252 | 0.0033 | 0.0036 | 0.012 | 0.0038 | 0.0018 | 0.0053 | 0.0029 | 0.0029 | 0.001 | 0.0016 | 0.0017 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.003 | 0.004 | 0.0014 | 0.002 | 0.002 | 0.0017 | 0.0044 | 0.0028 | 0.002 | 0.0093 | 0.0026 | 0.0008 | 0.0026 | 0.0018 | 0.0019 | 0.0012 | 0.0002 | 0.0011 |
Endosome | 0.0109 | 0.0105 | 0.0054 | 0.0011 | 0.0029 | 0.0082 | 0.0173 | 0.005 | 0.0033 | 0.014 | 0.0125 | 0.0022 | 0.0071 | 0.002 | 0.0043 | 0.0024 | 0.0013 | 0.0031 |
Golgi | 0.0038 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0015 | 0.0022 | 0.0002 | 0.0026 | 0.0013 | 0.0067 | 0.0001 | 0.0034 | 0.0001 | 0.0003 | 0 | 0.0025 | 0.0003 |
Lipid Particles | 0.0088 | 0.0053 | 0.0026 | 0.0001 | 0.0003 | 0.0067 | 0.0077 | 0.0021 | 0.0019 | 0.0033 | 0.003 | 0.0004 | 0.0073 | 0.0012 | 0.003 | 0.0002 | 0.0196 | 0.0027 |
Mitochondria | 0.0042 | 0.0006 | 0.0008 | 0.001 | 0.0008 | 0.0008 | 0.001 | 0.0009 | 0.0019 | 0.0077 | 0.0078 | 0.0004 | 0.0015 | 0.0004 | 0.0087 | 0.0008 | 0.0157 | 0.0004 |
None | 0.0699 | 0.0849 | 0.0371 | 0.0094 | 0.0395 | 0.0474 | 0.0494 | 0.0877 | 0.0518 | 0.0662 | 0.1127 | 0.0497 | 0.0259 | 0.0776 | 0.0782 | 0.0818 | 0.0626 | 0.0306 |
Nuclear Periphery | 0.0276 | 0.0365 | 0.0334 | 0.0801 | 0.0465 | 0.0201 | 0.0327 | 0.0292 | 0.0362 | 0.0457 | 0.027 | 0.0125 | 0.0134 | 0.0227 | 0.0154 | 0.0078 | 0.0136 | 0.0132 |
Nucleolus | 0.0345 | 0.0679 | 0.0864 | 0.0541 | 0.0181 | 0.0702 | 0.0258 | 0.0615 | 0.044 | 0.0258 | 0.0227 | 0.0307 | 0.0595 | 0.0939 | 0.125 | 0.0896 | 0.101 | 0.0727 |
Nucleus | 0.7466 | 0.6866 | 0.7382 | 0.8104 | 0.8352 | 0.746 | 0.6395 | 0.6383 | 0.626 | 0.5723 | 0.6009 | 0.7471 | 0.7861 | 0.7297 | 0.6924 | 0.7751 | 0.6867 | 0.8071 |
Peroxisomes | 0.0105 | 0.0002 | 0.0094 | 0 | 0 | 0.0102 | 0.0065 | 0.0003 | 0.0016 | 0.0058 | 0.0006 | 0.0001 | 0.0056 | 0.0002 | 0.0005 | 0 | 0.0194 | 0.0003 |
Punctate Nuclear | 0.0302 | 0.0492 | 0.0527 | 0.0269 | 0.0299 | 0.0541 | 0.0545 | 0.0429 | 0.0869 | 0.0199 | 0.1294 | 0.0432 | 0.0182 | 0.0347 | 0.0351 | 0.0083 | 0.0679 | 0.0441 |
Vacuole | 0.0032 | 0.0054 | 0.0043 | 0.001 | 0.0011 | 0.0014 | 0.0039 | 0.0064 | 0.0036 | 0.0192 | 0.0056 | 0.0014 | 0.0018 | 0.0019 | 0.0039 | 0.0022 | 0.0004 | 0.0013 |
Vacuole Periphery | 0.0013 | 0.0012 | 0.001 | 0.001 | 0.0005 | 0.0005 | 0.002 | 0.0007 | 0.0008 | 0.0046 | 0.0047 | 0.0003 | 0.0005 | 0.0003 | 0.0008 | 0.0004 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.104 | 11.3938 | 9.6625 | 9.2424 | 10.6234 | 7.2792 | 6.583 | 7.22 | 6.088 | 10.7451 |
Translational Efficiency | 1.3711 | 1.0894 | 1.1811 | 1.5167 | 1.0507 | 1.2338 | 1.7042 | 1.1825 | 1.9548 | 0.818 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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