Standard name
Human Ortholog
Description Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.96 1.0 1.0 0.98 0.93 0.82 – 0.78 0.67 0.62 0.63 0.99 0.99 1.0 – – – 0.97 0.92 0.98 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.1 – 0.3 0.4 0.5 0.37 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 – 0.06 0.06 0.1 0.08 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 – – – 2 0 0 0 0 0
Bud 0 8 1 0 0 0 1 – 1 2 1 1 0 0 0 – – – 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 2 4 0 1 1 1 – 2 2 3 1 2 3 2 – – – 0 0 0 0 0 0
Cytoplasm 235 305 235 87 161 117 135 – 129 99 96 148 353 443 467 – – – 224 318 232 272 413 435
Endoplasmic Reticulum 4 1 0 0 0 0 0 – 0 0 0 1 0 0 0 – – – 0 0 0 0 2 2
Endosome 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 – – – 1 4 0 0 2 4
Golgi 1 0 0 0 0 0 1 – 0 0 0 0 1 0 0 – – – 0 2 0 0 1 0
Mitochondria 0 13 0 0 1 9 17 – 50 59 78 88 0 2 1 – – – 0 3 0 2 4 3
Nucleus 3 0 1 0 0 6 3 – 5 5 4 7 0 2 0 – – – 0 2 0 1 1 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 2 0 2 0 0 0 – – – 0 2 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 3
Vac/Vac Membrane 1 1 1 0 2 1 21 – 10 9 16 18 2 1 1 – – – 1 7 0 1 1 0
Unique Cell Count 241 318 236 87 164 126 165 166 147 156 236 356 447 469 232 345 237 285 435 463
Labelled Cell Count 245 330 242 87 166 134 179 198 180 198 267 359 451 471 232 345 237 285 435 463


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 4.8 7.5 6.8 7.6 5.8 5.9 – 5.0 5.1 4.9 5.4 7.4 7.4 7.1 – – – 8.2 8.1 7.9
Std Deviation (1e-4) 0.7 0.7 0.9 1.0 2.1 1.0 1.6 – 1.1 0.9 0.7 1.0 1.3 1.1 1.1 – – – 1.3 1.3 1.4
Intensity Change (Log2) – – – -0.14 0.01 -0.39 -0.35 – -0.58 -0.58 -0.63 -0.47 -0.03 -0.03 -0.08 – – – 0.12 0.11 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0458 6.409 6.0298 5.6746 6.0134 6.0387 4.7109 5.4594 5.2784 5.005 4.6702 5.3624 2.043 3.1598 2.7507 2.5855 2.86 2.7685
Actin 0.0502 0.0057 0.0155 0.0025 0.0188 0.0068 0.0702 0.0045 0.0188 0.0065 0.0051 0.001 0.0339 0.0009 0.0107 0.0004 0.0286 0.0073
Bud 0.0009 0.0034 0.0015 0.0016 0.0025 0.0006 0.0008 0.0053 0.0029 0.0006 0.0008 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0016
Bud Neck 0.0017 0.0003 0.0013 0.0029 0.0012 0.0009 0.0022 0.0004 0.0004 0.0006 0.0015 0.0004 0.0015 0.0001 0.0003 0.0002 0.0007 0.0021
Bud Periphery 0.0013 0.0067 0.002 0.0063 0.006 0.0017 0.0011 0.0056 0.0031 0.0005 0.0016 0 0.0002 0 0.0001 0.0001 0.0004 0.0008
Bud Site 0.0104 0.0083 0.0114 0.0008 0.0051 0.0018 0.0114 0.0035 0.0021 0.0006 0.0101 0.0001 0.0008 0.0004 0.0021 0.0001 0.0007 0.0004
Cell Periphery 0.0007 0.0008 0.0007 0.0003 0.0005 0.0001 0.0004 0.0004 0.0002 0.0001 0.0011 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.5637 0.8323 0.715 0.8534 0.7481 0.8225 0.4508 0.8067 0.6308 0.6848 0.7893 0.7286 0.6867 0.8019 0.7426 0.7017 0.5372 0.7679
Cytoplasmic Foci 0.0215 0.0047 0.0098 0.008 0.0074 0.0081 0.0338 0.0056 0.0168 0.0073 0.0221 0.0112 0.0143 0.0042 0.0046 0.004 0.0075 0.0125
Eisosomes 0.0005 0.0001 0.0003 0.0001 0.0002 0 0.0005 0.0003 0.0001 0.0001 0.0004 0 0.0003 0 0 0 0.0003 0.0005
Endoplasmic Reticulum 0.0056 0.0077 0.0038 0.0031 0.01 0.0014 0.0049 0.0026 0.0049 0.0105 0.0016 0.0019 0.0059 0.001 0.0024 0.0017 0.0021 0.0027
Endosome 0.0323 0.0046 0.0149 0.002 0.0296 0.0116 0.0156 0.0016 0.0153 0.014 0.0471 0.0083 0.0147 0.0005 0.0015 0.001 0.0188 0.0044
Golgi 0.012 0.0004 0.0067 0.0006 0.0082 0.0068 0.0074 0.0004 0.0027 0.0096 0.0068 0.0013 0.005 0.0001 0.0005 0.0001 0.0115 0.0015
Lipid Particles 0.0162 0.0004 0.006 0.0027 0.0214 0.0041 0.0086 0.0009 0.0011 0.0058 0.0029 0.0019 0.0055 0 0.0003 0.0001 0.0198 0.0045
Mitochondria 0.0111 0.0004 0.0102 0.0033 0.0334 0.008 0.0171 0.0007 0.0015 0.0181 0.0179 0.0004 0.0015 0.0001 0.0002 0.0001 0.0083 0.0008
None 0.1944 0.1162 0.1722 0.0903 0.0801 0.1163 0.3256 0.1545 0.2726 0.2302 0.0828 0.2387 0.2066 0.1863 0.2257 0.2858 0.3468 0.1768
Nuclear Periphery 0.0087 0.0013 0.0032 0.0019 0.0083 0.001 0.0069 0.0014 0.0043 0.003 0.0008 0.0008 0.0034 0.0004 0.001 0.0006 0.0033 0.0031
Nucleolus 0.004 0.0001 0.0005 0.002 0.002 0.0001 0.0005 0.0002 0.0004 0.0001 0.0002 0 0.0003 0 0.0001 0 0.0003 0.0004
Nucleus 0.0116 0.0019 0.0065 0.0035 0.004 0.0021 0.0044 0.003 0.0036 0.0034 0.0023 0.0019 0.0023 0.0027 0.0025 0.0026 0.0014 0.0034
Peroxisomes 0.0294 0.0002 0.0126 0.005 0.003 0.0032 0.019 0.0004 0.0058 0.0014 0.0027 0.0019 0.0083 0.0001 0.0008 0.0001 0.0073 0.0028
Punctate Nuclear 0.0159 0.0012 0.0035 0.0086 0.0018 0.002 0.016 0.0013 0.0032 0.0008 0.001 0.0009 0.0075 0.0007 0.004 0.0009 0.0033 0.0054
Vacuole 0.0065 0.0031 0.0016 0.0009 0.0052 0.0006 0.0018 0.0007 0.0077 0.0014 0.0014 0.0003 0.0008 0.0003 0.0004 0.0004 0.0007 0.0005
Vacuole Periphery 0.0015 0.0002 0.0008 0.0003 0.0033 0.0003 0.001 0.0001 0.0016 0.0007 0.0004 0.0001 0.0003 0 0.0001 0.0001 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.5483 31.6776 27.559 23.4929 15.8429 28.0889 23.7458 24.5993 16.5372 26.1155
Translational Efficiency 1.0658 0.7538 0.7776 0.7708 0.9164 0.8208 1.0077 0.6251 1.0514 0.8478

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Spe4

Spe4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available