Standard name
Human Ortholog
Description Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.13 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.19 0.34 0.31 0.24 0.31 0 0.07 0.05 0.05 0.07 0.06
Mitochondria 0.86 0.9 0.94 0.97 0.82 0.89 0.89 0.87 0.94 0.92 0.93 0.92 0.91 0.79 0.77 0.07 0.13 0.11 0.74 0.74 0.83 0.81 0.77 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.05 0 0 0.07 0.12 0.1 0 0.09 0.11 0.11 0.06 0.07 0 0.05 0.13 0.07 0 0 0 0 0 0
Nucleolus 0.21 0 0 0 0.06 0.12 0.15 0.14 0.09 0.08 0.11 0.08 0.05 0 0 0.09 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.16 0.11 0.18 0 0 0.05 0.06 0.06 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.13 0 0 0.09 0.05 0 0 0 0 0 0 0.05 0.09 0.09 0.14 0.17 0.22 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 0 1 2 1 2 1 1 1 3 2 0 3 4 3 0 4 5 3 8 18
Bud 2 3 3 4 9 11 7 11 14 11 10 15 2 1 4 2 6 3 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 1 0
Bud Site 1 0 1 0 0 1 2 6 3 2 2 5 0 0 0 1 0 0 – – – – – –
Cell Periphery 2 12 4 6 3 5 4 3 2 4 5 9 1 0 6 9 7 2 0 0 0 0 0 1
Cytoplasm 0 6 1 2 13 6 7 6 3 8 2 6 0 4 4 3 6 1 0 1 0 0 0 0
Endoplasmic Reticulum 0 4 1 1 1 8 2 5 3 8 12 2 2 1 2 0 2 1 0 1 0 0 0 0
Endosome 0 4 3 2 6 0 2 7 1 0 1 4 2 6 1 1 1 1 8 11 3 6 12 8
Golgi 0 8 6 2 12 3 1 3 0 1 2 4 19 32 67 18 13 14 3 25 10 11 27 25
Mitochondria 87 293 202 230 239 419 379 660 362 479 434 589 162 134 153 4 7 5 90 251 181 177 327 305
Nucleus 2 1 0 0 1 2 4 4 0 1 0 1 0 1 1 2 1 0 0 2 0 0 0 0
Nuclear Periphery 3 15 11 5 8 34 53 77 15 48 50 70 10 12 8 3 7 3 3 2 0 0 6 6
Nucleolus 21 11 8 5 18 56 65 107 36 42 51 51 9 6 4 5 3 2 0 1 0 1 3 3
Peroxisomes 1 4 8 8 17 1 5 17 8 6 0 7 3 2 12 9 6 8 4 9 11 14 25 27
SpindlePole 1 1 0 1 0 4 3 5 3 2 3 6 1 1 1 2 2 0 2 1 0 1 0 1
Vac/Vac Membrane 6 44 7 3 27 23 10 11 11 10 6 13 9 15 18 8 9 10 5 23 0 2 8 10
Unique Cell Count 101 327 216 238 291 471 427 756 384 519 465 640 179 169 200 58 54 45 123 338 219 220 426 413
Labelled Cell Count 126 407 256 270 354 574 546 923 463 623 579 784 223 217 281 70 75 53 123 338 219 220 426 413


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 8.6 11.0 11.4 12.2 10.7 10.7 11.1 10.9 11.5 10.9 10.9 13.5 13.1 13.4 12.1 11.4 12.7 12.0 12.2 12.9
Std Deviation (1e-4) 3.0 2.3 2.6 2.6 3.5 3.2 3.1 3.6 3.5 4.1 4.0 3.7 3.0 3.1 2.5 3.0 3.0 3.2 2.7 3.0 3.4
Intensity Change (Log2) – – – 0.05 0.14 -0.04 -0.04 0.0 -0.02 0.06 -0.02 -0.02 0.29 0.25 0.28 0.13 0.05 0.2 0.12 0.14 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.2 0 0 0 1.6 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.8 0 0 0 0 0 0 0 3.2 5.3 8.2 6.8 0 0
Mitochondria 1.5 -3.8 -1.9 -1.9 -2.5 0.4 -0.6 -0.1 -0.7 -1.1 -4.2 -4.9 -13.6 -12.7 -12.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 -1.4 1.0 2.9 2.3 -0.7 1.9 2.4 2.5 0.2 0.8 -0.5 0 0 0
Nucleolus -1.0 1.3 3.4 4.3 4.2 2.6 2.2 3.1 2.1 0.6 -0.1 -1.0 0 0 0
Peroxisomes -0.2 1.1 0 0 -1.2 -1.2 0 0 0 0 0 1.1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 1.0 -0.7 0 -0.3 0 0 -1.0 0.9 2.4 2.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.3133 11.1455 11.0282 10.2265 10.4455 10.8492 13.0829 14.3118 12.8772 12.0957 11.7235 13.6427 11.9414 14.1274 12.7201 11.1349 11.1449 12.8646
Actin 0.0036 0.0061 0.0016 0.0003 0.0003 0.0002 0.0073 0.0029 0.0013 0.0004 0.0005 0.0038 0.0273 0.0199 0.0154 0.0073 0.0186 0.0012
Bud 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0002 0.0001 0 0.0002 0 0.0006 0.0002 0.0005 0.0002 0.0032 0
Bud Neck 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0005 0 0 0 0.0003 0.0071 0.0002 0.0003 0.0001 0.0011 0
Bud Periphery 0.0002 0.0001 0.0002 0 0 0.0001 0.0003 0.0007 0.0001 0 0.0005 0.0001 0.001 0.0004 0.0006 0.0003 0.006 0.0001
Bud Site 0.0003 0.0001 0.0003 0 0 0 0.0011 0.0029 0.0005 0.0008 0.0001 0 0.0103 0.0008 0.0028 0.0005 0.0093 0
Cell Periphery 0.0003 0.0001 0.0001 0 0 0 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0007 0.0004 0.0003 0.0001 0.0005 0
Cytoplasm 0 0 0 0 0 0 0.0007 0 0 0.0001 0 0.0005 0.002 0.0001 0.0001 0 0.0004 0
Cytoplasmic Foci 0.0009 0.001 0.0008 0 0 0.0001 0.0131 0.0016 0.0039 0.0193 0.0001 0.0002 0.0024 0.0009 0.005 0.017 0.0488 0.0002
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0006 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0 0 0 0 0 0 0.0006 0 0.0001 0 0 0.0006 0.0024 0.0001 0.0001 0.0001 0.0003 0
Endosome 0.0015 0.0039 0.0019 0.0003 0.0001 0.0006 0.0092 0.005 0.0023 0.0009 0.0003 0.0014 0.0074 0.0026 0.0041 0.0054 0.0089 0.0008
Golgi 0.0031 0.0271 0.0063 0.0004 0.0003 0.001 0.0076 0.0208 0.0075 0.0008 0.0008 0.0016 0.0049 0.0234 0.0044 0.0145 0.0187 0.0023
Lipid Particles 0.0154 0.0022 0.0035 0.0003 0.0001 0.0048 0.0205 0.0095 0.0067 0.0122 0.0016 0.0041 0.0228 0.015 0.003 0.08 0.0425 0.0025
Mitochondria 0.9535 0.9416 0.9721 0.9973 0.9952 0.9885 0.904 0.941 0.9598 0.9492 0.9894 0.9723 0.8773 0.8777 0.922 0.8424 0.7754 0.9839
None 0 0 0 0 0 0 0.0076 0 0 0.0002 0.0001 0.0001 0.002 0 0 0.0001 0.0004 0
Nuclear Periphery 0.0001 0.0001 0 0 0 0.0001 0.0013 0.0002 0.0001 0.0001 0 0.0017 0.0075 0.0003 0.0001 0.0001 0.0011 0.0002
Nucleolus 0.0043 0.0002 0.0003 0 0 0.0001 0.0018 0.0004 0.0006 0.0021 0.0002 0.0002 0.0011 0.0032 0.0003 0.0006 0.0015 0
Nucleus 0.0004 0 0.0001 0 0 0 0.0022 0.0002 0.0001 0.0002 0.0001 0.0003 0.0018 0.002 0.0001 0.0001 0.0005 0
Peroxisomes 0.0083 0.0053 0.0047 0.0002 0.0023 0.0006 0.0098 0.0101 0.009 0.004 0.0035 0.0015 0.0137 0.0059 0.0148 0.0285 0.032 0.0011
Punctate Nuclear 0 0 0 0 0 0 0.0032 0 0.0001 0.0008 0 0.0002 0.0007 0 0.0002 0.0004 0.012 0
Vacuole 0.0027 0.0027 0.0033 0.0002 0.0001 0.0006 0.0022 0.001 0.001 0.0009 0.0006 0.0016 0.0041 0.0212 0.0131 0.0006 0.007 0.0004
Vacuole Periphery 0.005 0.0091 0.0045 0.0008 0.0012 0.0031 0.0067 0.0026 0.0066 0.0076 0.0016 0.0094 0.0024 0.0251 0.0127 0.0015 0.0118 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.7895 26.9851 31.617 41.1798 28.3239 34.1398 46.8505 49.6728 44.901 44.0919
Translational Efficiency 1.2099 1.5338 1.1902 0.8839 1.0006 1.269 1.14 0.9353 1.323 1.0887

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mas1

Mas1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mas1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available