Standard name
Human Ortholog
Description Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 – 0.05 0.08 0 0.05 0.11 0.06 0.06 0 0.07 0.16 0.1 0.08 0.14 0.11 0.11 0 0 0 0 0 0
Bud 0.05 0 – 0 0 0.09 0.13 0.09 0.09 0.1 0.05 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.13 – 0.05 0.07 0.08 0.09 0.09 0.09 0.05 0.11 0.07 0.07 0.09 0.09 0.36 0.36 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.08 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.79 0.45 – 0.58 0.58 0.59 0.4 0.36 0.23 0.2 0.08 0.17 0.67 0.8 0.8 0.29 0.25 0.29 0.89 0.79 0.77 0.81 0.89 0.88
Golgi 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0.16 0.23 0.26 0 0 0 0 0 0
Mitochondria 0.12 0.18 – 0.05 0 0.18 0.33 0.27 0.54 0.64 0.75 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0.11 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 – 0.4 0.4 0.28 0.23 0.26 0.22 0.13 0.06 0.15 0.25 0.22 0.23 0.07 0.07 0 0 0 0.07 0.08 0 0
Vac/Vac Membrane 0 0.16 – 0 0 0 0 0 0 0 0.05 0.07 0.05 0.06 0.05 0.16 0.09 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 6 – 5 17 7 13 24 5 7 3 14 54 47 37 41 19 23 3 2 1 4 3 6
Bud 14 3 – 4 8 16 35 20 7 13 6 20 18 4 4 3 3 7 2 0 0 2 1 1
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 – 0 0 0 1 2 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 3 2 6 0 0 0 0 0 0
Cytoplasm 17 12 – 5 15 15 24 19 7 6 14 14 24 41 41 101 62 76 3 3 0 5 3 4
Endoplasmic Reticulum 9 8 – 0 4 2 6 4 2 2 5 6 24 15 16 11 5 4 0 1 0 1 0 4
Endosome 217 43 – 62 128 110 110 77 18 25 10 33 225 375 376 83 43 59 265 98 41 233 466 500
Golgi 4 0 – 3 10 1 4 2 1 0 0 3 4 2 3 45 39 53 3 2 1 3 9 13
Mitochondria 34 17 – 5 9 33 93 58 42 81 94 108 4 1 1 2 5 8 2 2 1 5 4 4
Nucleus 0 0 – 2 0 0 2 2 0 1 1 1 1 0 0 2 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 – 0 1 1 1 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 9 4 – 12 2 5 13 4 1 4 3 2 7 2 2 2 3 2 0 1 0 0 0 0
Peroxisomes 5 3 – 6 4 8 15 4 0 4 1 3 7 11 7 0 0 0 2 2 0 1 11 8
SpindlePole 17 3 – 42 88 52 63 55 17 17 7 29 84 105 110 19 12 8 10 2 3 22 16 16
Vac/Vac Membrane 12 15 – 2 9 4 11 2 2 2 6 14 16 28 24 44 15 16 1 3 0 3 2 2
Unique Cell Count 276 95 106 221 188 278 211 78 127 125 198 334 471 469 283 170 206 300 125 54 287 523 570
Labelled Cell Count 355 115 148 295 254 392 273 103 162 151 248 470 631 621 356 208 262 300 125 54 287 523 570


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.3 – 7.3 8.0 6.6 6.3 6.6 5.6 5.4 5.4 5.7 7.9 8.9 8.9 15.8 17.3 17.2 7.7 8.3 8.6
Std Deviation (1e-4) 0.9 1.5 – 1.1 3.2 1.1 1.5 1.2 1.3 0.9 1.1 0.9 1.2 1.2 1.2 3.1 3.2 3.0 1.2 1.3 1.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1864 3.6794 2.8477 2.936 2.2024 2.9288 2.9026 4.2927 3.8152 3.168 3.6742 3.8557 2.4131 3.9633 3.2882 3.2347 2.871 3.4766
Actin 0.0059 0.0004 0.002 0.0035 0.002 0.0021 0.027 0.0008 0.0036 0.0312 0.0074 0.0277 0.0153 0.0003 0.0006 0.0013 0.0001 0.0006
Bud 0.0002 0.0001 0.0005 0.0004 0.001 0.0001 0.0007 0.0019 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001
Bud Neck 0.0009 0.0003 0.0003 0.0004 0.0003 0.0004 0.0146 0.0003 0.0003 0.0008 0.0003 0.0008 0.0004 0.0002 0.0002 0.0004 0.0002 0.0003
Bud Periphery 0.0002 0.0001 0.0003 0.0002 0.0018 0.0002 0.0009 0.0005 0.0002 0.0007 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0
Bud Site 0.0014 0.0009 0.0007 0.0007 0.0018 0.0002 0.0039 0.0065 0.0029 0.0025 0.0004 0.0005 0.0005 0.0013 0.0003 0.0004 0.0001 0.0001
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0005 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0219 0.0405 0.0276 0.0438 0.0121 0.0133 0.0173 0.024 0.027 0.0358 0.0179 0.0126 0.0113 0.045 0.0409 0.0593 0.0134 0.0098
Cytoplasmic Foci 0.2824 0.3672 0.3288 0.2302 0.3584 0.326 0.3128 0.3506 0.3528 0.2828 0.2581 0.3384 0.3288 0.3377 0.3787 0.2644 0.2952 0.3551
Eisosomes 0.0001 0 0 0 0 0 0.0007 0.0001 0 0.0002 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0.0019 0.0013 0.0008 0.001 0.001 0.0017 0.0007 0.0043 0.0017 0.0013 0.0022 0.0014 0.0008 0.001 0.0009 0.0007 0.0004
Endosome 0.5131 0.4786 0.4763 0.5838 0.4701 0.4633 0.4544 0.4749 0.4585 0.4169 0.5503 0.449 0.4628 0.461 0.4453 0.5388 0.5775 0.4433
Golgi 0.0613 0.0483 0.0607 0.0848 0.057 0.0794 0.0556 0.0489 0.0518 0.0632 0.0886 0.069 0.0697 0.0571 0.0764 0.1049 0.0858 0.1066
Lipid Particles 0.0271 0.0166 0.0166 0.0047 0.0088 0.0207 0.0324 0.0127 0.0267 0.028 0.0196 0.0141 0.0478 0.0294 0.0087 0.0054 0.004 0.0179
Mitochondria 0.004 0.0009 0.0076 0.0093 0.0076 0.0073 0.006 0.0009 0.0031 0.0294 0.0052 0.0011 0.001 0.0005 0.0007 0.0043 0.0007 0.0007
None 0.0056 0.0016 0.0156 0.0007 0.0006 0.0004 0.009 0.0078 0.0013 0.0014 0.0259 0.001 0.0027 0.0007 0.0005 0.0008 0.0002 0.0001
Nuclear Periphery 0.004 0.0007 0.0018 0.0004 0.0011 0.0013 0.002 0.0011 0.0021 0.0009 0.0076 0.0019 0.0013 0.0006 0.0003 0.0003 0.0002 0.0002
Nucleolus 0.0013 0.0005 0.0002 0.0001 0.0002 0.0001 0.0007 0.0006 0.0001 0.0001 0.0001 0.0022 0.0002 0.0001 0 0 0 0
Nucleus 0.0042 0.0003 0.0005 0.0002 0.0004 0.0002 0.0009 0.001 0.0003 0.0003 0.0006 0.0034 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001
Peroxisomes 0.054 0.0334 0.0492 0.0261 0.0688 0.0771 0.0494 0.0572 0.0578 0.0933 0.0108 0.0653 0.0468 0.0506 0.0414 0.0084 0.0184 0.0607
Punctate Nuclear 0.0033 0.0022 0.0043 0.0009 0.0013 0.0018 0.0057 0.0016 0.0023 0.0025 0.0012 0.0078 0.0053 0.0089 0.0008 0.0004 0.0002 0.0007
Vacuole 0.0055 0.0043 0.004 0.0058 0.0036 0.0033 0.003 0.0067 0.0031 0.0052 0.0026 0.0019 0.0032 0.0041 0.003 0.0066 0.0021 0.002
Vacuole Periphery 0.002 0.0011 0.0017 0.0031 0.0021 0.0017 0.001 0.0012 0.0014 0.0024 0.0016 0.0008 0.0009 0.001 0.0012 0.0029 0.0011 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.1121 24.8332 19.9015 21.164 30.975 13.3268 21.8447 16.6079 26.3613 23.7486
Translational Efficiency 2.9179 1.3183 1.1985 1.3366 1.2173 3.3712 1.7074 1.6206 1.068 1.5042

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
Localization
Cell Percentages endosome (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vta1

Vta1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vta1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available