Standard name
Human Ortholog
Description Putative transcription factor, contains Forkhead Associated domain; binds chromatin; involved in expression homeostasis, buffering of mRNA synthesis rate against gene dosage changes during S phase; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase complexes; APCC(Cdh1) substrate; relative distribution to nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.79 0.83 0.88 0.84 0.8 0.76 0.73 0.73 0.64 0.77 0.6 0.58 0.46 0.5 0.49 – 0.82 0.81 0.52 0.63 0.62 0.81 0.85 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.31 0.09 0.16 0.11 0.15 0.33 0.34 0.36 0.45 0.46 0.53 0.64 0 0 0 – 0 0.05 0.16 0 0.15 0.05 0.06 0.06
Nucleus 0.1 0.07 0.08 0.16 0.09 0.07 0.1 0.1 0 0.05 0 0 0.48 0.47 0.46 – 0.06 0.08 0.1 0.1 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.05 0.07 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 3 1 2 2 1
Bud 0 0 0 0 1 0 1 0 3 1 0 1 0 0 0 – 0 1 4 3 0 1 4 2
Bud Neck 0 0 0 0 0 0 2 1 1 1 2 1 0 0 0 – 0 2 0 1 0 0 0 0
Bud Site 0 0 0 0 1 0 2 1 0 1 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 0 0 1 1 0 0 0 0 1 0 – 2 4 0 1 0 0 0 0
Cytoplasm 129 57 22 16 74 58 91 95 74 88 50 69 66 107 135 – 129 101 86 57 36 111 176 118
Endoplasmic Reticulum 1 1 0 0 0 0 1 0 1 0 0 0 1 0 0 – 13 8 1 0 0 0 1 0
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 – 2 2 5 2 0 0 1 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 2 0 0
Mitochondria 50 6 4 2 14 25 43 47 52 53 44 76 4 3 3 – 5 6 26 2 8 6 13 7
Nucleus 16 5 2 3 8 5 12 13 5 6 3 5 69 100 126 – 10 10 16 9 3 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 2 0 2 – 0 1 0 0 0 0 0 0
Nucleolus 1 0 0 0 2 0 3 1 0 1 0 0 12 9 10 – 0 1 0 0 0 1 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 – 0 1 1 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 3 1 2 1 0 1 0 0 2 – 2 2 4 1 1 1 3 1
Vac/Vac Membrane 6 1 0 0 4 2 1 0 1 0 0 1 2 0 2 – 14 6 11 3 1 0 2 2
Unique Cell Count 163 69 25 19 93 76 125 130 115 115 83 118 143 213 273 157 125 166 92 59 137 208 140
Labelled Cell Count 204 74 28 21 105 90 160 160 141 152 99 154 156 220 284 177 145 166 92 59 137 208 140


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.3 2.8 2.8 3.5 2.8 2.5 2.6 2.7 2.4 2.5 2.3 5.1 5.2 5.2 4.6 5.5 5.5 3.8 3.8 4.3
Std Deviation (1e-4) 0.6 1.5 0.3 0.4 1.7 1.4 1.0 1.3 1.4 1.4 1.1 1.0 1.6 2.0 2.0 1.5 2.2 1.7 1.6 1.3 0.8
Intensity Change (Log2) – – – -0.0 0.32 0.02 -0.17 -0.12 -0.04 -0.24 -0.17 -0.3 0.87 0.89 0.9 0.7 0.97 0.96 0.42 0.45 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4678 0.7971 0.344 0.1068 -0.1739 -0.0089 0.1691 0.9891 0.8923 0.7605 1.3263 0.4008 1.1649 1.9343 1.5715 0.9707 1.2084 1.4479
Actin 0.023 0.0003 0.0063 0.0018 0.0204 0.0011 0.01 0.0005 0.0103 0.0117 0.0149 0.0086 0.0432 0.0001 0.025 0.0765 0.038 0.0064
Bud 0.0145 0.0007 0.0053 0.0061 0.0058 0.0013 0.0086 0.0078 0.0024 0.0014 0.0006 0.0028 0.0062 0.0052 0.0024 0.0139 0.0053 0.0029
Bud Neck 0.0049 0.0008 0.0049 0.0021 0.0142 0.0082 0.0075 0.0009 0.0007 0.0002 0.0003 0.0037 0.0083 0.0007 0.0007 0.0024 0.0026 0.0116
Bud Periphery 0.0143 0.0005 0.0061 0.0074 0.0104 0.0015 0.0132 0.0061 0.0036 0.0025 0.0007 0.0037 0.0104 0.0024 0.0066 0.0209 0.0069 0.0058
Bud Site 0.0214 0.0037 0.0091 0.0407 0.0085 0.0017 0.0177 0.0234 0.0064 0.0014 0.0015 0.0008 0.0216 0.0075 0.0073 0.0088 0.0076 0.002
Cell Periphery 0.0021 0.0002 0.0004 0.0005 0.001 0.0004 0.002 0.0012 0.0004 0.0002 0.0002 0.0002 0.0035 0.0003 0.0003 0.0006 0.0008 0.0006
Cytoplasm 0.0507 0.0409 0.0557 0.0076 0.0406 0.0634 0.0312 0.0364 0.019 0.0087 0.0415 0.0558 0.038 0.0176 0.0343 0.0437 0.0168 0.0494
Cytoplasmic Foci 0.0129 0.0068 0.0068 0.0155 0.0071 0.0148 0.0356 0.0029 0.0147 0.0199 0.0163 0.0185 0.0176 0.0013 0.0126 0.0046 0.0052 0.0089
Eisosomes 0.0005 0.0004 0.0003 0.0003 0.0014 0.0001 0.0005 0.0003 0.0003 0.0004 0.0002 0.0001 0.001 0.0001 0.0005 0.0004 0.0016 0.0001
Endoplasmic Reticulum 0.0032 0.0006 0.0017 0.0007 0.0017 0.0009 0.003 0.0026 0.0007 0.0039 0.0025 0.0028 0.0032 0.0004 0.0018 0.0014 0.0009 0.0011
Endosome 0.0063 0.0044 0.0024 0.0009 0.0075 0.0019 0.0113 0.0022 0.0058 0.0488 0.0362 0.0041 0.0084 0.0002 0.0028 0.0009 0.0033 0.003
Golgi 0.0015 0.0004 0.0006 0.0005 0.0057 0.0002 0.0058 0.0001 0.0035 0.0199 0.0147 0.0015 0.0033 0 0.0014 0.0021 0.003 0.0014
Lipid Particles 0.0045 0.0005 0.0013 0.0019 0.01 0.0011 0.016 0.0006 0.003 0.0077 0.0024 0.0073 0.0077 0.0001 0.0054 0.0013 0.0117 0.0021
Mitochondria 0.004 0.0006 0.0008 0.0016 0.0053 0.0005 0.0062 0.0035 0.0069 0.0038 0.022 0.0022 0.0064 0.0007 0.014 0.0035 0.0033 0.0017
None 0.6264 0.459 0.6225 0.901 0.8183 0.6368 0.5657 0.5837 0.5683 0.8544 0.8381 0.6506 0.632 0.5017 0.608 0.7574 0.8324 0.6414
Nuclear Periphery 0.005 0.0014 0.0031 0.0003 0.0036 0.0021 0.0055 0.0119 0.0016 0.0019 0.0004 0.0042 0.0106 0.0008 0.0034 0.0025 0.002 0.0035
Nucleolus 0.0366 0.0162 0.011 0.0024 0.0051 0.0214 0.0277 0.0197 0.007 0.001 0.0001 0.0145 0.0252 0.0162 0.0057 0.0042 0.0142 0.0169
Nucleus 0.1266 0.4481 0.246 0.0012 0.0143 0.2159 0.1716 0.2797 0.3212 0.0009 0.0003 0.1905 0.1098 0.4345 0.2362 0.0364 0.0233 0.2195
Peroxisomes 0.0056 0.0002 0.0005 0.0025 0.0067 0.0009 0.02 0.0002 0.0125 0.0027 0.002 0.0085 0.0127 0.0001 0.0107 0.013 0.0094 0.0018
Punctate Nuclear 0.0154 0.0134 0.0128 0.0035 0.0071 0.0206 0.0225 0.0081 0.0097 0.006 0.0005 0.0162 0.0131 0.0091 0.0182 0.0019 0.0089 0.0159
Vacuole 0.0181 0.0009 0.0022 0.0014 0.0038 0.0048 0.0162 0.0066 0.0019 0.0018 0.0035 0.0028 0.0156 0.0009 0.0024 0.0029 0.0022 0.0035
Vacuole Periphery 0.0025 0.0001 0.0003 0.0002 0.0014 0.0003 0.0023 0.0017 0.0003 0.0008 0.0012 0.0006 0.0024 0.0001 0.0004 0.0008 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 105.8368 23.4851 11.452 23.6364 63.745 75.02 14.5867 9.3871 14.9391 61.7752
Translational Efficiency 1.2384 0.8711 0.622 0.9327 1.2901 1.817 1.1805 1.2488 1.2632 1.318

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative transcription factor, contains Forkhead Associated domain; binds chromatin; involved in expression homeostasis, buffering of mRNA synthesis rate against gene dosage changes during S phase; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase complexes; APCC(Cdh1) substrate; relative distribution to nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (23%), nucleus (20%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tos4

Tos4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tos4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available