Standard name
Human Ortholog
Description UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.95 0.92 0.89 0.86 0.85 0.79 – 0.71 0.69 0.98 0.99 1.0 0.97 0.94 0.94 0.96 0.83 0.87 0.93 0.89 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.17 0.08 0.26 0.27 0.31 0.42 – 0.49 0.57 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 – 0 1 0 0 0 0 1 2 0 0 2 1 3 3
Bud 0 0 0 2 6 10 6 5 5 – 6 10 1 1 3 1 0 2 1 0 2 3 3 7
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 1 0 0 0 – 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 1 2 2 2 6 3 6 11 – 7 9 1 8 11 13 7 14 0 0 0 0 4 4
Cytoplasm 385 174 305 271 365 336 468 446 267 – 221 266 260 396 420 418 265 427 379 163 274 174 286 291
Endoplasmic Reticulum 4 2 0 0 1 3 3 0 4 – 1 1 1 0 0 15 6 17 2 0 1 0 2 5
Endosome 0 0 1 1 12 4 3 3 3 – 0 2 1 1 0 1 1 1 2 2 3 0 1 4
Golgi 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 0 1 1
Mitochondria 1 9 11 47 33 97 146 161 142 – 154 219 3 0 14 8 4 7 3 1 15 1 4 2
Nucleus 1 1 0 0 1 1 2 2 2 – 1 2 0 0 0 1 2 3 0 1 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 – 1 2 0 0 1 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 1 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 1 1 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 8 5 3 2 1 – 0 1 0 0 0 0 1 2 0 0 3 0 1 2
Vac/Vac Membrane 1 0 0 0 0 0 5 3 1 – 4 7 0 3 1 6 12 23 0 24 1 0 1 1
Unique Cell Count 392 181 312 284 398 379 543 525 340 312 384 265 399 421 429 282 454 395 196 314 187 321 334
Labelled Cell Count 398 187 319 324 431 465 642 629 438 395 522 267 409 451 463 300 500 395 196 314 187 321 334


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.7 4.0 3.8 4.2 3.9 3.9 4.3 3.8 – 3.8 3.7 5.5 5.8 5.0 7.5 8.3 8.5 5.4 5.0 5.8
Std Deviation (1e-4) 0.7 0.8 0.8 1.2 1.3 0.9 0.9 1.3 1.1 – 1.2 1.1 1.1 1.7 1.3 1.5 1.6 2.3 1.6 1.4 1.2
Intensity Change (Log2) – – – -0.06 0.08 -0.03 -0.04 0.09 -0.08 – -0.08 -0.1 0.45 0.54 0.32 0.92 1.06 1.09 0.44 0.31 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.7 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0732 4.1147 3.7611 3.3906 4.0011 3.9396 2.4804 2.6786 2.4053 2.9397 2.0241 2.6737 0.8622 1.3866 1.5272 1.5285 0.8481 1.7245
Actin 0.0005 0.0004 0.0006 0.0018 0.0002 0.0005 0.0336 0.0013 0.0119 0.0085 0.0377 0.0024 0.0005 0.0002 0.0324 0.0004 0.0002 0.0063
Bud 0 0.0001 0.0003 0.0003 0.0001 0 0.0013 0.0002 0.0006 0.001 0.0008 0.0001 0.0002 0.0001 0.002 0.0003 0 0.0023
Bud Neck 0.0001 0.0003 0.0009 0.0007 0.0002 0.0092 0.0038 0.0001 0.0002 0.0026 0.0008 0.0007 0.0001 0.0001 0.0054 0.0003 0.0002 0.0003
Bud Periphery 0 0.0001 0.0015 0.0005 0 0.0001 0.0017 0.0001 0.0007 0.0009 0.0012 0.0001 0.0002 0 0.0025 0.0003 0 0.0075
Bud Site 0.0001 0.0069 0.0012 0.0009 0.0001 0.0001 0.0105 0.0007 0.0011 0.0165 0.0028 0.0002 0.0001 0.0003 0.0089 0.0001 0 0.0001
Cell Periphery 0.0006 0.0016 0.0059 0.0013 0.0004 0.0007 0.0003 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0002 0.0014 0.0001 0 0.0001
Cytoplasm 0.6245 0.8009 0.8083 0.8256 0.8116 0.8751 0.4737 0.7145 0.6577 0.6481 0.531 0.7618 0.403 0.4716 0.4938 0.3496 0.5484 0.4763
Cytoplasmic Foci 0.0027 0.0091 0.0043 0.0073 0.0033 0.003 0.0423 0.0096 0.0088 0.0308 0.0437 0.0136 0.0054 0.0023 0.0126 0.0031 0.0047 0.0028
Eisosomes 0.0002 0.0001 0.0001 0.0003 0 0 0.0003 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0004 0 0 0.0001
Endoplasmic Reticulum 0.0037 0.0027 0.0046 0.004 0.0022 0.0031 0.006 0.0011 0.0022 0.0018 0.002 0.0013 0.0019 0.0014 0.0061 0.0007 0.0006 0.001
Endosome 0.0009 0.0019 0.002 0.0026 0.0022 0.0006 0.0517 0.0013 0.0041 0.0153 0.0577 0.005 0.0007 0.0005 0.0056 0.0004 0.0007 0.0011
Golgi 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001 0.0172 0.0019 0.0014 0.0327 0.0168 0.004 0.0001 0.0001 0.0062 0 0 0.0004
Lipid Particles 0.0005 0.0002 0.0005 0.0005 0 0.0001 0.0289 0.0005 0.0011 0.0414 0.0193 0.0011 0.0003 0.0001 0.0018 0 0 0.0001
Mitochondria 0.0002 0.0001 0.0004 0.0003 0.0001 0.0001 0.018 0.0014 0.0033 0.0055 0.0296 0.0038 0.0001 0.0001 0.0096 0.0001 0.0001 0.0004
None 0.3641 0.1714 0.1646 0.1503 0.1769 0.1052 0.2336 0.259 0.2958 0.1628 0.2367 0.1945 0.5843 0.5214 0.4046 0.6432 0.4432 0.5001
Nuclear Periphery 0.0003 0.0005 0.0006 0.0005 0.0004 0.0002 0.0112 0.0003 0.0011 0.0012 0.0011 0.0003 0.0003 0.0004 0.0009 0.0002 0.0002 0.0002
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0 0.0041 0 0.0001 0.0033 0.0003 0 0 0 0.0002 0 0 0
Nucleus 0.0005 0.0014 0.0011 0.001 0.0009 0.0007 0.0091 0.0007 0.0012 0.0026 0.0014 0.0009 0.0007 0.0006 0.0012 0.0005 0.0009 0.0003
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0242 0.0058 0.0054 0.0134 0.0089 0.0091 0.0002 0 0.0019 0 0 0.0001
Punctate Nuclear 0.0002 0.0008 0.0003 0.0003 0.0001 0.0002 0.0201 0.0008 0.0023 0.0064 0.0034 0.0005 0.0002 0.0002 0.0011 0.0002 0.0003 0.0001
Vacuole 0.0005 0.001 0.0022 0.0013 0.0009 0.0006 0.0047 0.0006 0.0008 0.0044 0.0034 0.0003 0.0011 0.0004 0.0011 0.0003 0.0002 0.0004
Vacuole Periphery 0 0.0001 0.0001 0.0001 0.0001 0 0.0038 0.0001 0.0002 0.0004 0.0012 0.0001 0.0001 0 0.0003 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5892 13.4369 16.2788 19.1951 11.534 11.0371 18.7154 26.7604 25.9837 14.7431
Translational Efficiency 0.5317 0.4403 0.4702 0.3898 0.3349 0.485 0.43 0.2938 0.3222 0.4113

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg26

Atg26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available