Standard name
Human Ortholog
Description N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.91 0.97 0.95 0.96 0.94 0.81 0.77 0.75 0.73 0.7 0.57 0.63 0.99 0.99 0.99 0.95 – 0.91 0.66 0.86 0.64 0.83 0.76 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.2 0.05 0 0.05 0.12 0.37 0.45 0.35 0.38 0.53 0.6 0.56 0 0 0 0 – 0.07 0.17 0 0.2 0.05 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 2 10 4 5 6
Bud 0 0 0 0 2 10 8 8 7 16 13 9 0 0 0 0 – 0 0 2 7 5 19 13
Bud Neck 0 2 4 0 1 2 4 4 6 7 1 1 2 3 0 0 – 0 0 1 2 2 3 6
Bud Site 0 0 0 0 0 1 1 0 0 8 5 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 4 1 0 0 1 1 1 2 1 2 3 1 1 1 – 1 0 1 6 3 3 5
Cytoplasm 60 296 390 213 221 328 378 368 274 343 179 239 430 632 603 140 – 162 58 253 257 412 424 431
Endoplasmic Reticulum 0 0 4 1 0 1 1 0 0 0 0 1 0 1 3 7 – 13 1 0 4 1 6 3
Endosome 0 0 0 0 0 0 0 0 0 1 0 1 2 0 2 1 – 4 0 3 6 5 6 5
Golgi 0 0 2 1 1 0 0 0 0 0 0 0 0 1 0 0 – 1 1 0 3 2 2 3
Mitochondria 13 16 8 12 28 148 221 171 144 257 188 211 1 1 0 6 – 13 15 11 79 25 26 30
Nucleus 0 1 0 1 2 2 2 1 0 2 3 1 0 2 1 0 – 1 0 0 0 4 2 3
Nuclear Periphery 2 0 0 1 0 1 0 0 1 0 0 1 0 0 0 0 – 0 0 0 0 3 1 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0 0 1 2 0
Peroxisomes 0 0 2 0 0 1 0 1 1 2 0 1 2 0 2 0 – 0 0 0 1 0 0 0
SpindlePole 0 0 3 5 1 5 10 20 13 7 9 7 0 1 0 0 – 2 1 4 6 8 27 9
Vac/Vac Membrane 0 0 0 1 0 1 2 2 1 1 4 2 1 3 1 4 – 6 3 4 3 3 7 5
Unique Cell Count 66 305 409 222 234 403 489 489 376 489 314 379 434 638 611 148 178 90 294 403 495 555 543
Labelled Cell Count 75 315 417 237 256 500 628 576 448 646 404 477 441 645 613 159 203 90 294 403 495 555 543


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.5 3.8 3.9 4.1 3.1 3.0 3.3 3.1 2.7 2.4 2.5 5.1 5.1 4.9 5.3 4.9 4.9 5.1 5.2 5.4
Std Deviation (1e-4) 0.6 0.6 1.0 2.0 2.4 1.7 1.4 1.8 1.8 1.1 1.0 1.1 1.3 1.3 1.3 1.7 1.9 1.9 1.5 1.6 1.6
Intensity Change (Log2) – – – 0.04 0.12 -0.26 -0.35 -0.2 -0.3 -0.48 -0.67 -0.6 0.43 0.45 0.39 0.5 0.37 0.39 0.42 0.47 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9815 3.0383 3.1482 2.8389 3.5858 3.0891 1.2471 -0.3805 -0.4177 0.7576 0.4682 -0.3812 0.0611 -0.6936 -0.4432 -0.8836 -0.2309 -0.689
Actin 0.0456 0.0094 0.0153 0.0092 0.0032 0.0019 0.0456 0.0033 0.0198 0.1036 0.0069 0.0046 0.0364 0.0016 0.0248 0.0001 0.0287 0.0085
Bud 0.0045 0.0237 0.0102 0.0302 0.0035 0.0082 0.0053 0.0052 0.0015 0.0013 0.0017 0.0015 0.004 0.009 0.003 0 0.0011 0.0008
Bud Neck 0.0105 0.0006 0.0014 0.0013 0.0003 0.0008 0.0152 0.0001 0.0016 0.0026 0.0008 0.0007 0.0041 0.0004 0.002 0 0.0028 0.0006
Bud Periphery 0.006 0.0095 0.0057 0.0147 0.004 0.0054 0.0162 0.0052 0.0024 0.0021 0.0021 0.0032 0.0031 0.0036 0.0074 0.0001 0.0023 0.001
Bud Site 0.0079 0.0299 0.0064 0.0141 0.0014 0.0008 0.0216 0.0038 0.0173 0.0044 0.0231 0.0004 0.0052 0.0209 0.0076 0.0001 0.0259 0.0002
Cell Periphery 0.001 0.0007 0.0005 0.0008 0.0001 0.0002 0.0015 0.0003 0.0002 0.0004 0.0002 0.0002 0.0012 0.0006 0.0005 0 0.0003 0.0001
Cytoplasm 0.0137 0.015 0.0132 0.0257 0.0219 0.03 0.0326 0.0613 0.0423 0.0426 0.0192 0.1245 0.0328 0.0617 0.0392 0.0206 0.0312 0.0713
Cytoplasmic Foci 0.0077 0.0066 0.007 0.0108 0.0046 0.0066 0.0521 0.0054 0.0148 0.0272 0.0636 0.0113 0.0158 0.0118 0.0162 0.0018 0.0346 0.0065
Eisosomes 0.0023 0.0014 0.0004 0.0003 0.0001 0.0001 0.0004 0.0002 0.0002 0.0007 0.0002 0.0001 0.0004 0.0002 0.0004 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0028 0.0006 0.0011 0.0011 0.006 0.0005 0.0059 0.0009 0.0009 0.0019 0.0033 0.0016 0.0069 0.0008 0.0019 0.0001 0.0011 0.0009
Endosome 0.0116 0.0014 0.0033 0.0106 0.0112 0.001 0.031 0.002 0.0063 0.0237 0.065 0.0041 0.0186 0.0012 0.0057 0.0001 0.0073 0.0019
Golgi 0.0038 0.0014 0.0019 0.0147 0.0015 0.0003 0.0081 0.0002 0.0052 0.0236 0.0143 0.0019 0.0049 0.0002 0.0028 0 0.0042 0.0006
Lipid Particles 0.0085 0.0026 0.0012 0.0102 0.0059 0.0003 0.0201 0.0003 0.0059 0.0206 0.0137 0.0043 0.0052 0.0005 0.0042 0.0001 0.0141 0.0011
Mitochondria 0.0086 0.0059 0.0133 0.0159 0.025 0.0009 0.0053 0.0034 0.0036 0.0253 0.0081 0.001 0.0052 0.0009 0.0106 0.0001 0.0038 0.0005
None 0.8378 0.8599 0.8908 0.7846 0.824 0.908 0.6317 0.9057 0.8669 0.6792 0.675 0.8253 0.8036 0.8681 0.8503 0.9756 0.8044 0.891
Nuclear Periphery 0.0051 0.0012 0.0013 0.0028 0.0132 0.001 0.0251 0.0002 0.0007 0.0065 0.0105 0.0034 0.0191 0.0018 0.0023 0.0001 0.0031 0.0034
Nucleolus 0.0039 0.0031 0.0023 0.005 0.0085 0.0011 0.0025 0.0002 0.001 0.0009 0.0015 0.0019 0.0019 0.0015 0.0012 0.0001 0.0007 0.0004
Nucleus 0.0093 0.0094 0.0069 0.0276 0.0285 0.0087 0.0185 0.0005 0.0008 0.0011 0.0051 0.0021 0.0115 0.008 0.0033 0.0002 0.001 0.0018
Peroxisomes 0.0033 0.0027 0.0075 0.0073 0.0219 0.0006 0.0251 0.0007 0.0038 0.0167 0.019 0.0011 0.0036 0.0007 0.0077 0.0001 0.0223 0.0014
Punctate Nuclear 0.0038 0.0137 0.0079 0.009 0.0136 0.0228 0.0263 0.0006 0.0034 0.0109 0.0575 0.0054 0.0113 0.0054 0.0066 0.0005 0.0102 0.0073
Vacuole 0.0015 0.0011 0.0019 0.0031 0.0009 0.0006 0.0067 0.0005 0.001 0.0029 0.0067 0.0012 0.0036 0.001 0.0019 0.0001 0.0005 0.0004
Vacuole Periphery 0.0008 0.0003 0.0005 0.0011 0.0006 0.0002 0.003 0.0001 0.0003 0.002 0.0025 0.0004 0.0016 0.0002 0.0005 0 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.8476 38.2149 37.9878 48.6235 43.3994 54.8172 40.0971 48.4671 46.6177 55.1462
Translational Efficiency 1.7268 1.9525 1.9257 1.5207 1.8063 1.5696 1.9076 1.4921 1.4915 1.405

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nmt1

Nmt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nmt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available