Standard name
Human Ortholog
Description Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 – 0 0 0 0.06 0.1 0.22 0.28 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.09 0.1 – 0.19 0.19 0.16 0.2 0 0 0.07 0 0 0 0 0 0.07 0 0.05 0.05
Golgi 0 0 0.27 0 0 0 0 – 0 0 0 0 0 0.08 0.09 0 0 0.06 0 0 0 0 0 0
Mitochondria 0.08 0.05 0 0.11 0 0.05 0.06 – 0.07 0.06 0.16 0.09 0.05 0 0 0.1 0.14 0.12 0 0 0 0 0 0
Nucleus 0.08 0.08 0 0.05 0.05 0 0.07 – 0.08 0 0.05 0.05 0.05 0 0 0.1 0.13 0.08 0.12 0.13 0.1 0.05 0.1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.71 0.86 0.49 0.89 0.82 0.87 0.83 – 0.77 0.73 0.77 0.73 0.86 0.66 0.56 0.8 0.81 0.83 0.62 0.7 0.41 0.71 0.59 0.6
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.08 0.06 0.08 – 0.09 0.12 0.07 0.14 0 0 0 0 0 0 0.12 0.07 0.22 0.06 0.11 0.14
Vac/Vac Membrane 0.19 0.09 0.34 0 0.06 0.05 0 – 0 0.1 0 0.05 0 0 0.05 0 0 0.05 0 0 0.05 0.06 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 3 0 1 2 4 – 4 3 4 3 1 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 – 1 2 3 2 0 0 0 0 0 0 3 9 11 3 3 2
Bud Neck 0 1 0 3 0 1 5 – 0 4 0 0 2 0 0 1 0 0 1 2 2 0 0 1
Bud Site 0 0 0 0 0 1 3 – 8 7 7 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 2 7 10 4 13 6 8 – 6 8 8 19 13 40 52 16 15 11 3 3 8 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 3 3 2 0 0 1 0 0 0
Endosome 0 4 10 5 7 26 34 – 47 58 43 61 1 7 12 0 0 0 5 10 17 0 4 5
Golgi 7 11 61 3 4 2 0 – 1 2 1 0 4 15 16 10 15 21 0 1 1 0 0 0
Mitochondria 15 20 1 26 4 15 22 – 18 19 45 28 7 1 1 33 48 40 0 1 1 0 1 0
Nucleus 16 30 9 11 12 8 24 – 20 12 15 14 6 3 1 33 46 26 26 53 26 3 10 4
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 135 313 110 204 203 242 288 – 188 217 213 219 110 118 103 272 288 269 136 297 108 46 58 63
Peroxisomes 1 0 1 1 0 1 0 – 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 0
SpindlePole 0 2 2 7 19 17 26 – 21 37 18 41 5 7 8 2 0 0 26 30 58 4 10 14
Vac/Vac Membrane 35 32 76 5 15 13 14 – 9 29 11 15 1 6 9 13 10 15 8 10 12 3 6 5
Unique Cell Count 189 366 224 228 248 278 345 243 299 276 300 128 180 184 340 355 324 219 427 262 66 99 106
Labelled Cell Count 215 422 283 269 278 335 429 323 398 369 414 150 198 205 386 426 384 219 427 262 66 99 106


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.2 35.9 27.2 27.5 22.5 18.3 17.0 – 13.7 14.0 12.8 12.6 30.5 28.8 24.6 61.7 67.3 63.2 24.6 28.8 28.0
Std Deviation (1e-4) 6.8 9.7 6.0 8.1 5.9 3.9 3.8 – 3.2 3.6 2.8 2.8 9.6 7.6 7.2 22.3 24.5 23.5 8.1 9.2 8.9
Intensity Change (Log2) – – – 0.01 -0.27 -0.57 -0.68 – -0.99 -0.96 -1.09 -1.11 0.16 0.08 -0.14 1.18 1.31 1.22 -0.14 0.08 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 0.4 -1.5 -1.4 – -1.2 -1.1 -0.9 0.9 2.1 5.4 6.7 0.1 -0.1 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -1.3 -1.0 2.1 2.4 – 4.9 5.0 4.0 5.3 0 -0.3 0.9 0 0 0
Golgi -7.9 -8.1 -8.9 -10.3 – -8.5 -9.2 -9.1 -9.6 -5.6 -4.8 -4.8 -8.5 -8.0 -6.7
Mitochondria 4.9 0 3.1 3.5 – 3.8 3.5 6.1 4.4 0 0 0 4.5 5.5 5.2
Nucleus 0.4 0.4 -0.7 1.5 – 1.9 0 0.7 0.4 0.3 -1.4 0 2.5 3.6 1.9
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 9.3 7.5 9.2 8.7 – 6.3 5.5 6.5 5.6 6.9 3.3 1.4 7.7 8.1 8.5
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.6 3.0 3.6 – 3.9 4.9 3.2 5.3 0 0 0 0 0 0
Vacuole -8.8 -7.7 -8.5 -9.5 – -8.5 -6.8 -8.8 -8.6 -7.2 -7.6 -7.2 -9.6 -10.3 -9.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.215 27.3244 23.7728 24.8819 21.3832 24.6265 24.54 27.4458 25.0824 24.1481 21.5891 24.8886 24.6294 30.8251 27.5327 26.0423 22.0227 25.1247
Actin 0.0088 0 0.0082 0 0.0072 0 0.0061 0 0.0028 0.0013 0.0417 0.0004 0.0261 0 0.024 0.0029 0.0069 0
Bud 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0037 0.0021 0.017 0.0001 0.0683 0.0001
Bud Neck 0.0003 0.0002 0.0002 0.0002 0.0008 0.0002 0.0002 0.0001 0.0001 0.0002 0.0004 0.0003 0.0023 0.0002 0.0019 0.0001 0.0003 0.0005
Bud Periphery 0.0003 0.0001 0.0004 0 0.0002 0 0.0002 0 0.0001 0 0.0002 0.0001 0.0129 0.0027 0.0068 0.0002 0.0324 0.0002
Bud Site 0.0006 0.0003 0.0004 0.0001 0.0003 0.0001 0.0006 0.0001 0.0003 0.0003 0.0007 0.0003 0.0183 0.0017 0.0182 0.0002 0.0066 0.0003
Cell Periphery 0.0003 0.0011 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0003 0.0002 0.0047 0.002 0.0027 0.0014 0.002 0.0014
Cytoplasm 0.0006 0 0.001 0 0.0032 0 0.0025 0 0.0012 0.0002 0.002 0.0004 0.0005 0.0001 0.0001 0 0.0004 0
Cytoplasmic Foci 0.0063 0.0002 0.0027 0 0.0082 0.0009 0.0064 0 0.0045 0.0142 0.0145 0.0038 0.0095 0.0015 0.0056 0.0006 0.0052 0.0035
Eisosomes 0.0001 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0 0.0003 0 0.0003 0 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0015 0 0.0008 0 0.0017 0 0.0021 0 0 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0004 0.0018 0
Endosome 0.0034 0.0001 0.0064 0 0.0121 0 0.006 0 0.0002 0.0037 0.0039 0.0011 0.0016 0.0001 0.0004 0.0066 0.0153 0.0003
Golgi 0.0017 0 0.0007 0 0.0051 0 0.0017 0 0.0002 0.0021 0.0048 0.0004 0.0011 0 0.0004 0.0062 0.0076 0.0003
Lipid Particles 0.0034 0.0001 0.0023 0 0.0045 0.0002 0.0043 0.0001 0.0023 0.0076 0.0074 0.002 0.0053 0.0007 0.0031 0.0084 0.0385 0.0108
Mitochondria 0.0045 0 0.0014 0 0.003 0.0002 0.0061 0.0001 0.0071 0.0003 0.0018 0.0003 0.0059 0.007 0.02 0.0597 0.0226 0.0298
None 0.0006 0 0.0016 0 0.0141 0 0.0201 0 0.0089 0.0004 0.0057 0.0004 0.0008 0 0.0001 0.0002 0.0014 0.0001
Nuclear Periphery 0.0032 0.0002 0.0034 0 0.0066 0 0.007 0.0001 0.0005 0.0004 0.0011 0.0002 0.0005 0 0 0.0018 0.0071 0.0001
Nucleolus 0.8484 0.8377 0.8354 0.8297 0.8369 0.885 0.8102 0.8207 0.8386 0.817 0.796 0.8757 0.8145 0.8293 0.7994 0.7995 0.7109 0.8609
Nucleus 0.1036 0.1526 0.1255 0.1687 0.0851 0.1081 0.1155 0.1764 0.1267 0.1214 0.0817 0.108 0.0528 0.1361 0.0731 0.1025 0.0483 0.0644
Peroxisomes 0.0003 0 0.0007 0 0.0004 0 0.0026 0 0.0005 0.0167 0.0099 0.0017 0.0017 0.0005 0.0046 0.0007 0.003 0.0038
Punctate Nuclear 0.0085 0.0028 0.0067 0.0004 0.0083 0.0048 0.0058 0.0015 0.0043 0.014 0.0263 0.0038 0.0054 0.0013 0.0003 0.0002 0.0154 0.0171
Vacuole 0.0022 0.0034 0.001 0.0003 0.0008 0.0002 0.0011 0.0006 0.0009 0.0001 0.0004 0.0007 0.0242 0.0113 0.0167 0.0039 0.0026 0.0034
Vacuole Periphery 0.0012 0.0009 0.0006 0.0001 0.0011 0.0001 0.0008 0.0002 0.0003 0 0.0006 0.0003 0.0077 0.0033 0.0053 0.0044 0.0034 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.4403 105.4186 84.5677 64.0544 66.9437 59.886 60.6403 44.5251 49.9342 69.0665
Translational Efficiency 1.8836 1.6976 1.7063 1.9073 1.8563 1.8282 1.9217 1.676 1.3756 1.375

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
Localization
Cell Percentages nucleolus (46%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Pwp1

Pwp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pwp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available