Standard name
Human Ortholog
Description Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.95 0.68 0.59 0.65 0.51 0.55 0.42 0.41 0.54 0.43 0.4 0.45 0.62 0.62 0.57 0.06 0.07 0 0 0 0 0 0 0
Nucleus 0.15 0.18 0.07 0.06 0 0 0 0 0 0 0.05 0 0.08 0 0.05 0.08 0.19 0.17 0 0.12 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.95 0.92 0.95 0.96 0.95 0.96 0.93 0.95 0.94 0.93 0.96 0.92 0.93 0.94 0.9 0.94 0.88 0.89 0.93 0.83 0.87 0.91 0.91 0.89
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud Neck 0 1 0 0 0 0 1 3 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 2
Bud Site 0 0 0 0 0 0 1 0 2 2 0 3 1 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1 4 1 4 2 4 3 0 3 2 3 3 12 13 10 6 1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 19 119 115 77 104 116 138 174 109 92 69 100 73 156 150 16 10 3 0 0 0 1 0 1
Nucleus 3 31 13 7 3 5 2 3 7 4 8 5 9 8 13 21 26 17 1 20 8 0 3 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 19 160 186 113 194 203 306 402 189 199 166 205 110 235 237 238 122 90 47 137 169 77 144 172
Peroxisomes 0 1 0 2 3 0 13 6 1 3 3 3 1 2 3 1 0 0 0 0 1 0 0 1
SpindlePole 0 1 0 0 3 2 23 21 5 9 6 7 0 3 5 0 0 1 0 0 5 0 0 2
Vac/Vac Membrane 0 0 1 0 0 0 1 0 0 1 0 1 0 1 2 0 0 1 1 3 2 0 4 4
Unique Cell Count 20 174 195 118 204 211 330 422 201 215 173 224 118 250 262 252 139 101 51 166 196 85 159 194
Labelled Cell Count 41 314 320 200 312 329 490 612 313 314 255 327 197 418 423 289 166 113 51 166 196 85 159 194


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.7 55.2 42.6 42.2 35.4 31.4 25.3 26.0 24.7 22.8 22.6 21.8 52.0 51.9 46.1 45.4 59.4 63.4 41.8 52.1 54.3
Std Deviation (1e-4) 11.3 20.1 17.9 18.3 15.5 11.5 11.9 11.8 10.1 11.6 9.6 8.7 27.3 24.9 19.8 24.9 26.3 26.7 15.4 19.8 24.3
Intensity Change (Log2) – – – -0.01 -0.27 -0.44 -0.75 -0.71 -0.78 -0.9 -0.91 -0.97 0.29 0.29 0.12 0.09 0.48 0.58 -0.03 0.29 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 1.5 1.6 1.2 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 -1.6 -0.8 -3.8 -4.1 -1.0 -3.3 -3.7 -2.9 0.5 0.7 -0.4 -12.1 -9.6 -9.3
Nucleus -0.3 -2.6 -2.1 -4.0 -4.3 -1.4 -2.4 -0.8 -2.2 0.3 -1.7 -0.8 0.7 3.4 2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 -0.1 0.4 -1.2 -0.1 -0.6 -1.2 0.3 -1.6 -0.8 -0.6 -2.0 -0.4 -2.6 -2.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3.8 3.2 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0113 0 0.0118 0 0.0004 0 0.0018 0 0 0 0 0 – – – – – –
Bud 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Bud Neck 0.0001 0 0.0004 0 0.0002 0 0.0004 0.0001 0.0001 0.0001 0 0 – – – – – –
Bud Periphery 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Bud Site 0.0003 0 0.002 0 0.0001 0 0.0005 0.0001 0 0.0002 0 0 – – – – – –
Cell Periphery 0.0001 0.0005 0.0003 0.0002 0.0003 0.0002 0.0003 0.0002 0.0004 0.0003 0.0009 0.0002 – – – – – –
Cytoplasm 0.0005 0 0 0 0 0 0.0005 0 0 0 0 0 – – – – – –
Cytoplasmic Foci 0.0436 0 0.0049 0.0004 0.0004 0.0003 0.1256 0.0001 0.0002 0.0007 0.0002 0.0008 – – – – – –
Eisosomes 0.0002 0 0 0 0.0006 0 0.0003 0 0 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0005 0 0 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Endosome 0.0066 0 0.0001 0 0 0 0.0043 0 0 0 0 0 – – – – – –
Golgi 0.0083 0 0.0006 0 0.0001 0 0.0037 0 0 0 0 0 – – – – – –
Lipid Particles 0.1845 0 0.0008 0.0005 0.0063 0.0013 0.0228 0.0001 0.0003 0.0004 0.0022 0.0009 – – – – – –
Mitochondria 0.0069 0.0054 0.0078 0.0028 0.3227 0.0234 0.0133 0.0008 0.0072 0.0072 0.2169 0.0266 – – – – – –
None 0.0038 0 0 0 0 0 0.0054 0 0 0 0 0 – – – – – –
Nuclear Periphery 0.0027 0.0001 0 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Nucleolus 0.6534 0.8101 0.8994 0.9698 0.6495 0.9414 0.755 0.8975 0.9627 0.955 0.7268 0.9372 – – – – – –
Nucleus 0.012 0.1823 0.0603 0.0259 0.0114 0.0321 0.0391 0.1002 0.028 0.0343 0.0476 0.0323 – – – – – –
Peroxisomes 0.052 0.0001 0.0108 0.0001 0.0068 0.0008 0.0224 0 0.0003 0.0003 0.0023 0.0007 – – – – – –
Punctate Nuclear 0.0121 0 0.0001 0 0 0 0.0036 0 0 0.0007 0 0.0008 – – – – – –
Vacuole 0.0002 0.0006 0.0003 0.0002 0.0003 0.0002 0.0004 0.0005 0.0004 0.0003 0.001 0.0002 – – – – – –
Vacuole Periphery 0.0007 0.0007 0.0002 0.0001 0.0009 0.0001 0.0004 0.0003 0.0003 0.0002 0.0019 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 447.9167 457.7046 447.3733 334.2323 350.3347 353.7675 305.451 272.5829 239.3467 300.0886
Translational Efficiency 1.2699 1.3137 1.1413 1.4629 1.3152 1.3377 1.3428 1.2422 1.1869 1.2437

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
Localization
Cell Percentages nucleolus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Nop56

Nop56


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop56-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available