Standard name | |
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Human Ortholog | |
Description | Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 4 | 1 | 3 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 0 | 3 | 7 |
Bud Neck | 0 | 0 | 7 | 4 | 8 | 3 | 2 | – | 4 | 6 | 6 | 5 | 5 | 8 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 3 | 3 |
Bud Site | 2 | 2 | 0 | 0 | 0 | 10 | 3 | – | 22 | 15 | 16 | 23 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 8 | 11 | 3 | 5 | 11 | – | 11 | 14 | 4 | 4 | 19 | 39 | 47 | 21 | 14 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3 | 5 | 4 | 7 | 26 | 12 | 43 | – | 33 | 50 | 25 | 30 | 5 | 9 | 8 | 2 | 3 | 1 | 4 | 2 | 3 | 1 | 5 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | 31 | 20 | 0 | 4 | 7 | 47 | 11 | – | 65 | 26 | 42 | 52 | 3 | 7 | 1 | 20 | 17 | 18 | 2 | 1 | 0 | 1 | 2 | 3 |
Nucleus | 68 | 93 | 48 | 18 | 29 | 18 | 29 | – | 47 | 38 | 20 | 45 | 26 | 16 | 25 | 42 | 30 | 49 | 38 | 74 | 34 | 11 | 28 | 26 |
Nuclear Periphery | 1 | 4 | 0 | 0 | 1 | 0 | 3 | – | 3 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 4 |
Nucleolus | 224 | 317 | 286 | 214 | 344 | 301 | 413 | – | 475 | 494 | 342 | 448 | 281 | 390 | 288 | 197 | 150 | 158 | 230 | 302 | 275 | 163 | 288 | 257 |
Peroxisomes | 6 | 0 | 3 | 1 | 8 | 5 | 22 | – | 14 | 30 | 11 | 24 | 7 | 16 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 8 | 39 | 69 | 144 | 132 | 243 | – | 190 | 279 | 161 | 245 | 36 | 65 | 48 | 7 | 4 | 0 | 6 | 11 | 25 | 21 | 50 | 41 |
Vac/Vac Membrane | 0 | 4 | 3 | 0 | 2 | 3 | 4 | – | 2 | 0 | 1 | 1 | 1 | 2 | 2 | 6 | 8 | 3 | 5 | 10 | 5 | 1 | 10 | 14 |
Unique Cell Count | 279 | 381 | 331 | 237 | 394 | 350 | 503 | 570 | 620 | 411 | 572 | 313 | 453 | 357 | 254 | 192 | 206 | 299 | 416 | 353 | 209 | 403 | 375 | |
Labelled Cell Count | 339 | 456 | 398 | 328 | 572 | 540 | 784 | 870 | 954 | 632 | 888 | 384 | 553 | 431 | 298 | 229 | 242 | 299 | 416 | 353 | 209 | 403 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.7 | 14.2 | 14.4 | 12.4 | 11.6 | 7.8 | 9.5 | – | 7.9 | 8.6 | 7.9 | 8.1 | 14.4 | 14.0 | 13.6 | 20.5 | 20.2 | 18.3 | 13.6 | 14.3 | 15.1 |
Std Deviation (1e-4) | 7.2 | 4.1 | 3.9 | 3.3 | 3.0 | 2.3 | 2.4 | – | 2.4 | 2.2 | 2.1 | 2.3 | 5.2 | 5.4 | 5.5 | 10.4 | 8.6 | 8.5 | 3.6 | 4.5 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.32 | -0.89 | -0.6 | – | -0.86 | -0.75 | -0.87 | -0.84 | 0.0 | -0.04 | -0.08 | 0.51 | 0.49 | 0.35 | -0.08 | -0.01 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | -0.1 | 0 | 0 | – | 0 | 0 | -0.7 | 0 | -0.5 | -0.4 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 3.6 | 2.9 | 3.6 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -1.8 | -0.9 | -0.2 | – | -0.5 | -0.2 | -1.5 | 0 | 2.3 | 3.6 | 5.2 | 3.2 | 2.7 | 1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.6 | 1.9 | 4.5 | – | 3.3 | 4.4 | 3.4 | 3.1 | 0 | 0.8 | 1.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.9 | 0 | – | 6.4 | 3.8 | 6.0 | 5.7 | 0 | 0 | 0 | 5.2 | 5.5 | 5.5 |
Nucleus | -2.5 | -3.1 | -4.1 | -4.3 | – | -2.9 | -4.3 | -4.5 | -3.2 | -2.5 | -5.5 | -3.2 | 0.7 | 0.3 | 2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.4 | 0.4 | -0.2 | -1.6 | – | -1.2 | -2.6 | -1.2 | -3.0 | 1.3 | -0.1 | -2.0 | -2.8 | -2.5 | -2.9 |
Peroxisomes | 0 | 1.2 | 0 | 2.9 | – | 1.6 | 3.2 | 1.8 | 2.8 | 0 | 2.4 | 1.4 | 0 | 0 | 0 |
SpindlePole | 5.2 | 7.6 | 7.8 | 10.9 | – | 7.2 | 10.3 | 8.4 | 9.7 | -0.1 | 1.0 | 0.7 | -4.0 | -3.9 | -5.1 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1482 | 8.7125 | 7.5427 | 7.2778 | 6.8473 | 7.1678 | 11.3248 | 12.5994 | 11.4885 | 9.0148 | 10.1481 | 10.5933 | 13.5806 | 15.6366 | 13.2434 | 12.9909 | 12.527 | 13.6703 |
Actin | 0.0236 | 0 | 0.0152 | 0.0002 | 0.0316 | 0 | 0.0122 | 0 | 0.0055 | 0.0021 | 0.0283 | 0.0015 | 0.0038 | 0 | 0 | 0 | 0.0005 | 0 |
Bud | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0021 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0029 | 0.0001 | 0.0006 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Bud Periphery | 0.0007 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0033 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0026 | 0 | 0.0013 | 0.0005 | 0.0007 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0033 | 0.0018 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0025 | 0 | 0.0004 | 0.0011 | 0.0007 | 0.0006 | 0.0011 | 0 | 0.0008 | 0.0003 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0 | 0.0032 | 0 |
Cytoplasmic Foci | 0.0177 | 0 | 0.0088 | 0.0263 | 0.0111 | 0.0025 | 0.0085 | 0 | 0.0003 | 0.0033 | 0.0018 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0012 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0022 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0 |
Endosome | 0.0158 | 0 | 0.0032 | 0.001 | 0.0044 | 0.0004 | 0.0048 | 0 | 0.0006 | 0.006 | 0.0133 | 0.0022 | 0.0004 | 0 | 0 | 0 | 0.0027 | 0 |
Golgi | 0.004 | 0 | 0.0027 | 0.0005 | 0.0041 | 0.0001 | 0.0023 | 0 | 0.0004 | 0.0025 | 0.0036 | 0.0011 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0195 | 0.0001 | 0.004 | 0.0239 | 0.0301 | 0.0014 | 0.0066 | 0 | 0.0009 | 0.0025 | 0.0063 | 0.0023 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0004 |
Mitochondria | 0.0052 | 0.0001 | 0.005 | 0.0037 | 0.0179 | 0.0002 | 0.0036 | 0 | 0.0002 | 0.0129 | 0.0038 | 0.0072 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
None | 0.0032 | 0 | 0.0027 | 0.0023 | 0.0017 | 0.0003 | 0.0017 | 0 | 0.0003 | 0.0005 | 0.0032 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0025 | 0 |
Nuclear Periphery | 0.0062 | 0.0001 | 0.0008 | 0.0017 | 0.0032 | 0.0011 | 0.0077 | 0 | 0.0028 | 0.0004 | 0.0128 | 0.0038 | 0.0002 | 0 | 0.0001 | 0 | 0.0109 | 0.0001 |
Nucleolus | 0.7905 | 0.8926 | 0.8466 | 0.8705 | 0.7908 | 0.8986 | 0.8564 | 0.8338 | 0.8939 | 0.8156 | 0.8038 | 0.8745 | 0.9048 | 0.8669 | 0.9266 | 0.8893 | 0.8593 | 0.905 |
Nucleus | 0.0713 | 0.1045 | 0.0966 | 0.0559 | 0.0893 | 0.0859 | 0.0811 | 0.1657 | 0.0913 | 0.1374 | 0.1042 | 0.0993 | 0.0812 | 0.1325 | 0.0715 | 0.1041 | 0.1092 | 0.0887 |
Peroxisomes | 0.0207 | 0 | 0.0036 | 0.0009 | 0.002 | 0.0001 | 0.0017 | 0 | 0.0001 | 0.004 | 0.0012 | 0.0005 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0078 | 0.0023 | 0.0056 | 0.0078 | 0.0049 | 0.0081 | 0.0087 | 0.0002 | 0.0019 | 0.0008 | 0.0075 | 0.0049 | 0.0063 | 0.0002 | 0.0006 | 0.0059 | 0.0081 | 0.0052 |
Vacuole | 0.0024 | 0.0001 | 0.001 | 0.001 | 0.001 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0017 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0007 | 0.0019 | 0.0027 | 0.0002 | 0.0007 | 0 | 0.0001 | 0.0008 | 0.0014 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.2708 | 44.6324 | 32.0152 | 30.8757 | 53.9446 | 46.5735 | 38.3586 | 25.3818 | 31.7979 | 43.8863 |
Translational Efficiency | 3.0146 | 2.5793 | 2.4523 | 2.3657 | 1.8093 | 2.0992 | 1.6525 | 2.366 | 1.6937 | 1.9466 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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