Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.08 0 0 0 0.08 0.09 0.05 0.14 0.17 0.11 0 0 0 0.18 0.13 0.15 0 0 0 0 0 0
Nucleus 0.2 0.25 0.17 0.11 0.09 0.06 0.08 0.09 0.07 0.09 0.1 0.11 0 0 0.1 0.12 0.1 0.1 0.19 0.12 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.85 0.88 0.93 0.88 0.87 0.87 0.79 0.85 0.85 0.81 0.91 0.91 0.86 0.83 0.8 0.83 0.84 0.74 0.78 0.84 0.75 0.76
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.18 0.18 0.3 0.34 0.32 0.38 0.25 0.3 0.39 0.05 0.07 0.08 0 0 0 0 0 0.06 0.07 0.1 0.1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 2 3 4 5 3 4 0 0 1 0 0 0 0 1 1 3 4 3
Bud Neck 1 0 3 6 8 7 15 11 6 5 7 7 8 2 0 0 0 1 0 0 1 9 6
Bud Site 0 0 0 0 2 14 10 12 12 12 18 1 2 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 1 5 3 7 11 7 8 14 7 6 8 9 20 31 7 7 7 0 0 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Endosome 0 4 2 2 15 24 16 12 6 3 3 1 6 9 3 0 0 2 2 4 0 4 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 3
Mitochondria 19 23 1 7 6 31 41 27 58 49 37 6 9 8 29 20 17 1 1 0 0 2 1
Nucleus 54 67 63 23 31 22 38 47 29 26 34 22 11 15 16 18 11 26 52 45 5 14 11
Nuclear Periphery 1 1 1 0 3 2 2 2 0 1 0 0 0 1 0 1 0 0 0 0 0 0 1
Nucleolus 248 229 322 199 321 330 413 405 340 239 276 183 373 335 133 123 93 226 208 297 170 243 235
Peroxisomes 0 0 2 0 2 7 3 2 1 0 2 2 12 9 6 3 1 0 0 0 0 2 1
SpindlePole 7 3 65 38 110 128 154 197 102 84 133 11 28 32 0 1 2 8 6 22 13 32 31
Vac/Vac Membrane 1 2 2 0 1 0 0 4 0 0 0 1 4 1 3 9 4 0 5 2 1 7 7
Unique Cell Count 269 271 364 215 364 378 476 512 402 280 339 202 411 389 160 154 112 271 282 380 202 324 309
Labelled Cell Count 332 335 464 282 511 574 703 737 566 428 522 243 473 445 197 185 137 271 282 380 202 324 309


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.1 15.2 13.7 11.7 11.1 8.6 8.2 8.5 7.3 6.2 7.0 15.7 15.8 15.0 26.9 28.4 24.1 16.2 14.9 15.9
Std Deviation (1e-4) 4.2 5.0 3.5 3.1 3.1 2.1 2.6 2.1 1.7 1.5 1.7 5.8 5.4 6.5 13.2 13.4 10.9 5.0 3.6 4.7
Intensity Change (Log2) -0.23 -0.3 -0.67 -0.74 -0.68 -0.92 -1.14 -0.98 0.2 0.2 0.13 0.97 1.05 0.81 0.24 0.12 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 1.5 0 2.3 1.5 0 0 0 0 1.3 0 0 0 0
Bud Site 0 0 3.7 0 0 3.3 4.0 4.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.2 0 0 2.0 0 0 1.6 0 3.3 4.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.2 4.3 2.8 2.1 0 0 0 0 0 2.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 5.5 4.1 7.3 8.1 6.2 0 0 0 8.1 6.7 0
Nucleus -2.2 -3.5 -4.9 -4.1 -3.6 -4.3 -2.9 -2.8 -2.0 -6.9 -6.1 -2.2 -1.6 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.6 -0.1 -0.5 -0.7 -3.6 -1.6 -1.2 -2.6 0.8 1.0 -1.0 -1.7 -2.6 -1.5
Peroxisomes 0 0 0 0 0 0 0 0 0 2.5 2.0 0 0 0
SpindlePole -0.1 3.9 5.0 4.7 6.6 2.5 3.6 6.3 -4.1 -4.7 -3.9 -5.7 -5.4 -4.3
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.3546 15.4285 13.4837 11.9994 11.6497 13.0427 15.1565 17.1018 14.8677 12.5808 13.7035 14.4519 12.1523 13.4765 11.7908 12.7249 10.7385 11.8849
Actin 0.0001 0 0.0003 0 0.0076 0.0002 0.0085 0 0.0018 0.0111 0.0005 0.0004 0.0042 0 0.0088 0 0.0082 0.0006
Bud 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0002 0 0.0012 0
Bud Neck 0.0008 0.0002 0.0002 0.0006 0.0067 0.0002 0.0003 0.0001 0.0003 0.0013 0.0006 0.0001 0.0011 0.0001 0.0002 0.0005 0.0004 0.0001
Bud Periphery 0.0001 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0001 0.0004 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0017 0.0001
Bud Site 0.0035 0.0002 0.0004 0.0003 0.0013 0.0001 0.0014 0 0.0003 0.0015 0.0004 0 0.0006 0 0.001 0.0001 0.0052 0
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0003 0.0001 0.0002 0 0.0003 0.0004 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.0003 0 0.0002 0 0.0003 0 0.0006 0 0.0003 0.0052 0.0001 0 0.0012 0 0.0004 0 0.0009 0
Cytoplasmic Foci 0.0128 0.0001 0.0033 0.008 0.0117 0.0014 0.0106 0.0004 0.0125 0.0188 0.0013 0.0012 0.0039 0 0.0016 0.0002 0.0105 0.0009
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0 0 0.0001 0 0.0003 0 0.0015 0 0.0003 0.001 0.0019 0 0.0011 0 0.0004 0 0.0013 0.0007
Endosome 0.0022 0 0.0007 0.0025 0.0104 0.0003 0.0136 0 0.0059 0.0191 0.0103 0.0006 0.0066 0 0.0023 0 0.0213 0.0022
Golgi 0.0013 0 0.0005 0.0017 0.0077 0.0006 0.004 0 0.0026 0.0143 0.001 0.0002 0.0015 0 0.001 0 0.0067 0.0013
Lipid Particles 0.0304 0.0004 0.0098 0.055 0.0573 0.0116 0.0152 0.0004 0.0083 0.0397 0.0041 0.0041 0.0042 0.0001 0.0016 0.0004 0.0243 0.0069
Mitochondria 0.0023 0.0001 0.0012 0.0002 0.0046 0.0003 0.0046 0 0.0067 0.0187 0.005 0.002 0.0009 0.0001 0.0138 0.0006 0.0128 0.0009
None 0.0113 0 0.0002 0 0.001 0 0.0011 0 0.0007 0.0011 0.0002 0.0001 0.001 0 0.0005 0 0.0015 0
Nuclear Periphery 0.0003 0 0.002 0 0.0089 0.0002 0.0064 0 0.0049 0.0033 0.0139 0.0012 0.0022 0 0.0049 0 0.0069 0.004
Nucleolus 0.8304 0.9284 0.8945 0.847 0.7766 0.9153 0.8546 0.9296 0.8795 0.775 0.8991 0.926 0.8916 0.8957 0.8742 0.9308 0.8231 0.904
Nucleus 0.0776 0.07 0.0679 0.08 0.0503 0.0594 0.0614 0.0687 0.0588 0.0651 0.0511 0.0605 0.0726 0.1033 0.0838 0.0664 0.054 0.0727
Peroxisomes 0.0014 0 0.0008 0.0026 0.0148 0.0046 0.0015 0 0.0023 0.0029 0.001 0.0004 0.0032 0 0.0014 0.0001 0.0056 0.0042
Punctate Nuclear 0.0229 0.0002 0.0167 0.0011 0.0369 0.0056 0.0119 0.0006 0.0118 0.0126 0.0047 0.0028 0.003 0.0005 0.0023 0.0003 0.0112 0.0005
Vacuole 0.0012 0.0002 0.0005 0.0007 0.0017 0.0001 0.0012 0.0001 0.001 0.005 0.0012 0.0001 0.0004 0.0001 0.0004 0.0003 0.0014 0.0002
Vacuole Periphery 0.0005 0 0.0003 0.0001 0.001 0.0001 0.0011 0 0.0017 0.0031 0.0027 0.0002 0.0003 0 0.0005 0.0001 0.0013 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.8248 45.8219 40.8618 40.7391 44.3058 35.0521 36.9831 32.5043 26.2737 41.8096
Translational Efficiency 0.9708 1.1881 1.0873 0.7948 0.8621 1.1667 1.1533 0.7406 0.8447 0.8253

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
Localization
Cell Percentages nucleolus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp13

Utp13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available