Standard name | |
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Human Ortholog | |
Description | Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 4 | 5 | – | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 4 | 3 |
Bud Neck | 1 | 0 | 3 | 6 | 8 | 7 | 15 | 11 | 6 | – | 5 | 7 | 7 | 8 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 9 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 14 | 10 | 12 | 12 | – | 12 | 18 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 5 | 3 | 7 | 11 | 7 | 8 | 14 | 7 | – | 6 | 8 | 9 | 20 | 31 | 7 | 7 | 7 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 2 | 2 | 15 | 24 | 16 | 12 | 6 | – | 3 | 3 | 1 | 6 | 9 | 3 | 0 | 0 | 2 | 2 | 4 | 0 | 4 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Mitochondria | 19 | 23 | 1 | 7 | 6 | 31 | 41 | 27 | 58 | – | 49 | 37 | 6 | 9 | 8 | 29 | 20 | 17 | 1 | 1 | 0 | 0 | 2 | 1 |
Nucleus | 54 | 67 | 63 | 23 | 31 | 22 | 38 | 47 | 29 | – | 26 | 34 | 22 | 11 | 15 | 16 | 18 | 11 | 26 | 52 | 45 | 5 | 14 | 11 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 3 | 2 | 2 | 2 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 248 | 229 | 322 | 199 | 321 | 330 | 413 | 405 | 340 | – | 239 | 276 | 183 | 373 | 335 | 133 | 123 | 93 | 226 | 208 | 297 | 170 | 243 | 235 |
Peroxisomes | 0 | 0 | 2 | 0 | 2 | 7 | 3 | 2 | 1 | – | 0 | 2 | 2 | 12 | 9 | 6 | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
SpindlePole | 7 | 3 | 65 | 38 | 110 | 128 | 154 | 197 | 102 | – | 84 | 133 | 11 | 28 | 32 | 0 | 1 | 2 | 8 | 6 | 22 | 13 | 32 | 31 |
Vac/Vac Membrane | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 4 | 0 | – | 0 | 0 | 1 | 4 | 1 | 3 | 9 | 4 | 0 | 5 | 2 | 1 | 7 | 7 |
Unique Cell Count | 269 | 271 | 364 | 215 | 364 | 378 | 476 | 512 | 402 | 280 | 339 | 202 | 411 | 389 | 160 | 154 | 112 | 271 | 282 | 380 | 202 | 324 | 309 | |
Labelled Cell Count | 332 | 335 | 464 | 282 | 511 | 574 | 703 | 737 | 566 | 428 | 522 | 243 | 473 | 445 | 197 | 185 | 137 | 271 | 282 | 380 | 202 | 324 | 309 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.1 | 15.2 | 13.7 | 11.7 | 11.1 | 8.6 | 8.2 | 8.5 | 7.3 | – | 6.2 | 7.0 | 15.7 | 15.8 | 15.0 | 26.9 | 28.4 | 24.1 | 16.2 | 14.9 | 15.9 |
Std Deviation (1e-4) | 4.2 | 5.0 | 3.5 | 3.1 | 3.1 | 2.1 | 2.6 | 2.1 | 1.7 | – | 1.5 | 1.7 | 5.8 | 5.4 | 6.5 | 13.2 | 13.4 | 10.9 | 5.0 | 3.6 | 4.7 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.3 | -0.67 | -0.74 | -0.68 | -0.92 | – | -1.14 | -0.98 | 0.2 | 0.2 | 0.13 | 0.97 | 1.05 | 0.81 | 0.24 | 0.12 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 1.5 | 0 | 2.3 | 1.5 | 0 | – | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.7 | 0 | 0 | 3.3 | – | 4.0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.2 | 0 | 0 | 2.0 | 0 | – | 0 | 1.6 | 0 | 3.3 | 4.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.2 | 4.3 | 2.8 | 2.1 | 0 | – | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.3 | 5.5 | 4.1 | 7.3 | – | 8.1 | 6.2 | 0 | 0 | 0 | 8.1 | 6.7 | 0 |
Nucleus | -2.2 | -3.5 | -4.9 | -4.1 | -3.6 | -4.3 | – | -2.9 | -2.8 | -2.0 | -6.9 | -6.1 | -2.2 | -1.6 | -1.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.6 | -0.1 | -0.5 | -0.7 | -3.6 | -1.6 | – | -1.2 | -2.6 | 0.8 | 1.0 | -1.0 | -1.7 | -2.6 | -1.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.5 | 2.0 | 0 | 0 | 0 |
SpindlePole | -0.1 | 3.9 | 5.0 | 4.7 | 6.6 | 2.5 | – | 3.6 | 6.3 | -4.1 | -4.7 | -3.9 | -5.7 | -5.4 | -4.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.3546 | 15.4285 | 13.4837 | 11.9994 | 11.6497 | 13.0427 | 15.1565 | 17.1018 | 14.8677 | 12.5808 | 13.7035 | 14.4519 | 12.1523 | 13.4765 | 11.7908 | 12.7249 | 10.7385 | 11.8849 |
Actin | 0.0001 | 0 | 0.0003 | 0 | 0.0076 | 0.0002 | 0.0085 | 0 | 0.0018 | 0.0111 | 0.0005 | 0.0004 | 0.0042 | 0 | 0.0088 | 0 | 0.0082 | 0.0006 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0012 | 0 |
Bud Neck | 0.0008 | 0.0002 | 0.0002 | 0.0006 | 0.0067 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0013 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 |
Bud Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0017 | 0.0001 |
Bud Site | 0.0035 | 0.0002 | 0.0004 | 0.0003 | 0.0013 | 0.0001 | 0.0014 | 0 | 0.0003 | 0.0015 | 0.0004 | 0 | 0.0006 | 0 | 0.001 | 0.0001 | 0.0052 | 0 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0003 | 0.0052 | 0.0001 | 0 | 0.0012 | 0 | 0.0004 | 0 | 0.0009 | 0 |
Cytoplasmic Foci | 0.0128 | 0.0001 | 0.0033 | 0.008 | 0.0117 | 0.0014 | 0.0106 | 0.0004 | 0.0125 | 0.0188 | 0.0013 | 0.0012 | 0.0039 | 0 | 0.0016 | 0.0002 | 0.0105 | 0.0009 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0015 | 0 | 0.0003 | 0.001 | 0.0019 | 0 | 0.0011 | 0 | 0.0004 | 0 | 0.0013 | 0.0007 |
Endosome | 0.0022 | 0 | 0.0007 | 0.0025 | 0.0104 | 0.0003 | 0.0136 | 0 | 0.0059 | 0.0191 | 0.0103 | 0.0006 | 0.0066 | 0 | 0.0023 | 0 | 0.0213 | 0.0022 |
Golgi | 0.0013 | 0 | 0.0005 | 0.0017 | 0.0077 | 0.0006 | 0.004 | 0 | 0.0026 | 0.0143 | 0.001 | 0.0002 | 0.0015 | 0 | 0.001 | 0 | 0.0067 | 0.0013 |
Lipid Particles | 0.0304 | 0.0004 | 0.0098 | 0.055 | 0.0573 | 0.0116 | 0.0152 | 0.0004 | 0.0083 | 0.0397 | 0.0041 | 0.0041 | 0.0042 | 0.0001 | 0.0016 | 0.0004 | 0.0243 | 0.0069 |
Mitochondria | 0.0023 | 0.0001 | 0.0012 | 0.0002 | 0.0046 | 0.0003 | 0.0046 | 0 | 0.0067 | 0.0187 | 0.005 | 0.002 | 0.0009 | 0.0001 | 0.0138 | 0.0006 | 0.0128 | 0.0009 |
None | 0.0113 | 0 | 0.0002 | 0 | 0.001 | 0 | 0.0011 | 0 | 0.0007 | 0.0011 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0005 | 0 | 0.0015 | 0 |
Nuclear Periphery | 0.0003 | 0 | 0.002 | 0 | 0.0089 | 0.0002 | 0.0064 | 0 | 0.0049 | 0.0033 | 0.0139 | 0.0012 | 0.0022 | 0 | 0.0049 | 0 | 0.0069 | 0.004 |
Nucleolus | 0.8304 | 0.9284 | 0.8945 | 0.847 | 0.7766 | 0.9153 | 0.8546 | 0.9296 | 0.8795 | 0.775 | 0.8991 | 0.926 | 0.8916 | 0.8957 | 0.8742 | 0.9308 | 0.8231 | 0.904 |
Nucleus | 0.0776 | 0.07 | 0.0679 | 0.08 | 0.0503 | 0.0594 | 0.0614 | 0.0687 | 0.0588 | 0.0651 | 0.0511 | 0.0605 | 0.0726 | 0.1033 | 0.0838 | 0.0664 | 0.054 | 0.0727 |
Peroxisomes | 0.0014 | 0 | 0.0008 | 0.0026 | 0.0148 | 0.0046 | 0.0015 | 0 | 0.0023 | 0.0029 | 0.001 | 0.0004 | 0.0032 | 0 | 0.0014 | 0.0001 | 0.0056 | 0.0042 |
Punctate Nuclear | 0.0229 | 0.0002 | 0.0167 | 0.0011 | 0.0369 | 0.0056 | 0.0119 | 0.0006 | 0.0118 | 0.0126 | 0.0047 | 0.0028 | 0.003 | 0.0005 | 0.0023 | 0.0003 | 0.0112 | 0.0005 |
Vacuole | 0.0012 | 0.0002 | 0.0005 | 0.0007 | 0.0017 | 0.0001 | 0.0012 | 0.0001 | 0.001 | 0.005 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0014 | 0.0002 |
Vacuole Periphery | 0.0005 | 0 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0011 | 0 | 0.0017 | 0.0031 | 0.0027 | 0.0002 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0013 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.8248 | 45.8219 | 40.8618 | 40.7391 | 44.3058 | 35.0521 | 36.9831 | 32.5043 | 26.2737 | 41.8096 |
Translational Efficiency | 0.9708 | 1.1881 | 1.0873 | 0.7948 | 0.8621 | 1.1667 | 1.1533 | 0.7406 | 0.8447 | 0.8253 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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