Standard name
Human Ortholog
Description Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.14 0.16 0.07 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0 0 0.08 0.18 0.28 0.16 0.19 0.22 0.24 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.94 0.92 0.84 0.83 0.76 0.75 0.74 0.76 0.73 0.73 0.91 0.84 0.81 0.88 0.88 0.9 0.94 0.94 0.88 0.87 0.74 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.07 0 0.1 0.17 0.23 0.25 0.32 0.31 0.38 0.38 0.34 0 0 0 0 0 0 0 0 0 0 0.08 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 1 3 3 1 4 2 6 0 0 0 1 0 0 2 1 1 1 2 3
Bud Neck 0 0 0 0 1 0 0 1 4 3 3 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 2 1 0 5 6 17 21 16 25 29 26 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 2 13 8 17 14 15 5 6 10 6 5 23 65 84 26 38 11 1 0 1 3 3 4
Endoplasmic Reticulum 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 6 4 2 0 0 1 1 0 2
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 1 2 4 1 1 2 0 2 6 2 4 2
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 2 0 2 3
Mitochondria 22 15 8 10 27 88 178 113 94 148 146 153 0 0 1 5 4 4 0 0 3 1 4 8
Nucleus 279 307 379 247 274 397 488 529 368 498 449 470 264 399 430 341 450 276 281 319 371 350 275 364
Nuclear Periphery 0 0 0 2 1 8 8 4 8 6 3 4 0 1 2 4 1 1 0 0 1 0 0 2
Nucleolus 33 22 17 26 56 110 160 224 155 251 237 220 3 10 11 16 18 12 5 2 12 14 29 65
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 1 0 0 3 0 0 1 0 0 1 0 0 0
SpindlePole 1 0 0 1 1 0 1 0 2 0 0 3 1 8 13 2 2 2 1 1 1 1 2 2
Vac/Vac Membrane 1 2 1 0 1 0 9 4 1 1 1 3 1 4 4 9 9 7 4 8 15 20 44 75
Unique Cell Count 297 322 405 268 327 479 639 705 496 658 618 645 291 476 533 388 509 306 300 340 424 401 374 537
Labelled Cell Count 344 350 422 294 385 624 880 904 655 946 876 894 294 489 552 414 527 319 300 340 424 401 374 537


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 8.1 7.6 6.6 6.4 5.6 5.5 5.7 5.5 5.5 5.2 5.2 9.7 8.9 8.6 10.9 10.7 10.9 10.0 9.9 10.8
Std Deviation (1e-4) 1.7 2.0 2.0 1.6 1.8 1.8 1.5 1.3 1.3 1.6 1.2 1.3 2.7 2.3 2.8 3.0 2.8 2.7 1.8 1.7 2.2
Intensity Change (Log2) -0.2 -0.23 -0.42 -0.46 -0.41 -0.46 -0.45 -0.55 -0.53 0.35 0.24 0.19 0.52 0.51 0.52 0.4 0.39 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.9 3.1 3.3 3.6 4.1 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 1.3 -0.2 -0.8 -3.2 -2.1 -1.8 -2.6 -3.0 2.8 5.4 6.3 2.3 2.8 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 4.0 7.8 10.6 7.2 8.0 9.2 9.5 9.5 0 0 0 -0.8 -1.6 -0.7
Nucleus -0.7 -4.2 -4.8 -7.2 -7.7 -7.7 -7.5 -8.3 -8.3 -1.4 -4.5 -5.7 -2.8 -2.7 -1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 5.8 7.9 8.7 10.7 10.3 12.4 12.4 11.3 -2.5 -1.8 -1.9 -0.1 -0.5 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3497 5.8472 6.1571 5.3507 5.1294 5.7378 7.7225 7.8126 7.5192 7.6743 7.1854 7.3293 6.6933 5.9546 5.8229 5.7961 5.6561 5.7689
Actin 0.0001 0 0 0 0.0009 0 0.0069 0 0.0116 0 0.0098 0 0.0037 0 0.0003 0 0 0.0001
Bud 0 0 0 0 0.0002 0 0.0003 0 0.0001 0 0.0004 0 0 0 0 0 0 0
Bud Neck 0.0001 0.0001 0 0.0001 0.0005 0.0003 0.0006 0 0.0014 0 0.0007 0.0002 0.0001 0 0 0 0.0001 0.0002
Bud Periphery 0 0 0 0 0.0001 0 0.0008 0 0.0001 0 0.0007 0 0.0001 0 0 0 0 0
Bud Site 0.0001 0 0 0 0.0002 0 0.0013 0 0.0003 0 0.0022 0 0.0001 0 0.0002 0 0 0
Cell Periphery 0 0 0 0 0 0 0.0006 0 0.0001 0 0.0003 0 0 0 0 0 0 0
Cytoplasm 0.001 0 0 0 0.003 0 0.0021 0 0.002 0 0.0026 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasmic Foci 0.0011 0 0 0 0.0113 0 0.0097 0 0.003 0 0.0015 0 0.0004 0 0.0004 0 0 0
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0003 0 0 0 0.0002 0 0.0019 0 0.0012 0 0.002 0 0.0001 0 0.0001 0 0 0
Endosome 0.0005 0 0 0 0.0044 0 0.008 0 0.0036 0 0.0083 0 0.0002 0 0.0001 0 0 0.0001
Golgi 0.0001 0 0 0 0.0003 0 0.0017 0 0.0006 0 0.0015 0 0.0002 0 0.0001 0 0 0.0001
Lipid Particles 0.0008 0 0 0 0.0015 0 0.0083 0 0.0012 0 0.002 0 0.0002 0 0.0006 0 0 0.0012
Mitochondria 0.0002 0.0001 0 0 0.0012 0 0.0087 0 0.0004 0 0.0091 0.0001 0.0002 0 0.0005 0.0001 0.0001 0.0033
None 0.0007 0 0 0 0.0102 0 0.0014 0 0.0008 0 0.0028 0 0.0004 0 0.0002 0 0 0
Nuclear Periphery 0.0016 0.0071 0.0003 0.0006 0.0128 0.0001 0.0121 0.0008 0.0041 0.0001 0.0286 0.0003 0.0006 0.0004 0.0013 0.0005 0.0002 0.0001
Nucleolus 0.0253 0.0196 0.007 0.0228 0.1733 0.0685 0.0256 0.0088 0.0092 0.0127 0.0821 0.0388 0.0147 0.0084 0.0084 0.0041 0.0831 0.039
Nucleus 0.9619 0.9725 0.9926 0.9759 0.7224 0.9304 0.8979 0.9902 0.9508 0.9871 0.8282 0.9602 0.9778 0.9909 0.9858 0.9951 0.916 0.955
Peroxisomes 0 0 0 0 0.0006 0 0.0032 0 0.0003 0 0.0005 0 0.0006 0 0.0001 0 0 0.0006
Punctate Nuclear 0.0058 0.0005 0.0001 0.0005 0.0557 0.0006 0.0049 0.0001 0.0032 0.0001 0.0109 0.0001 0.0003 0.0002 0.0015 0.0001 0.0004 0.0002
Vacuole 0.0002 0 0 0 0.0005 0 0.002 0 0.0046 0 0.0035 0.0001 0.0001 0 0.0001 0 0 0
Vacuole Periphery 0.0001 0.0001 0 0 0.0006 0 0.0019 0 0.0014 0 0.0023 0 0.0001 0 0.0002 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9319 6.6423 12.9382 16.355 6.0211 6.4979 14.0616 13.5985 15.6754 17.3815
Translational Efficiency 1.3718 3.0012 1.4994 1.2543 2.7225 2.3999 1.3105 1.2914 1.1228 0.9203

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Bur2

Bur2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bur2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available