Standard name | |
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Human Ortholog | |
Description | Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 1 | 4 | 2 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 2 | 1 | 0 | 5 | 6 | 17 | 21 | 16 | 25 | 29 | 26 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 2 | 13 | 8 | 17 | 14 | 15 | 5 | 6 | 10 | 6 | 5 | 23 | 65 | 84 | 26 | 38 | 11 | 1 | 0 | 1 | 3 | 3 | 4 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 4 | 2 | 0 | 0 | 1 | 1 | 0 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | 1 | 2 | 0 | 2 | 6 | 2 | 4 | 2 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 0 | 2 | 3 |
Mitochondria | 22 | 15 | 8 | 10 | 27 | 88 | 178 | 113 | 94 | 148 | 146 | 153 | 0 | 0 | 1 | 5 | 4 | 4 | 0 | 0 | 3 | 1 | 4 | 8 |
Nucleus | 279 | 307 | 379 | 247 | 274 | 397 | 488 | 529 | 368 | 498 | 449 | 470 | 264 | 399 | 430 | 341 | 450 | 276 | 281 | 319 | 371 | 350 | 275 | 364 |
Nuclear Periphery | 0 | 0 | 0 | 2 | 1 | 8 | 8 | 4 | 8 | 6 | 3 | 4 | 0 | 1 | 2 | 4 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
Nucleolus | 33 | 22 | 17 | 26 | 56 | 110 | 160 | 224 | 155 | 251 | 237 | 220 | 3 | 10 | 11 | 16 | 18 | 12 | 5 | 2 | 12 | 14 | 29 | 65 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 3 | 1 | 8 | 13 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 2 |
Vac/Vac Membrane | 1 | 2 | 1 | 0 | 1 | 0 | 9 | 4 | 1 | 1 | 1 | 3 | 1 | 4 | 4 | 9 | 9 | 7 | 4 | 8 | 15 | 20 | 44 | 75 |
Unique Cell Count | 297 | 322 | 405 | 268 | 327 | 479 | 639 | 705 | 496 | 658 | 618 | 645 | 291 | 476 | 533 | 388 | 509 | 306 | 300 | 340 | 424 | 401 | 374 | 537 |
Labelled Cell Count | 344 | 350 | 422 | 294 | 385 | 624 | 880 | 904 | 655 | 946 | 876 | 894 | 294 | 489 | 552 | 414 | 527 | 319 | 300 | 340 | 424 | 401 | 374 | 537 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 8.1 | 7.6 | 6.6 | 6.4 | 5.6 | 5.5 | 5.7 | 5.5 | 5.5 | 5.2 | 5.2 | 9.7 | 8.9 | 8.6 | 10.9 | 10.7 | 10.9 | 10.0 | 9.9 | 10.8 |
Std Deviation (1e-4) | 1.7 | 2.0 | 2.0 | 1.6 | 1.8 | 1.8 | 1.5 | 1.3 | 1.3 | 1.6 | 1.2 | 1.3 | 2.7 | 2.3 | 2.8 | 3.0 | 2.8 | 2.7 | 1.8 | 1.7 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.23 | -0.42 | -0.46 | -0.41 | -0.46 | -0.45 | -0.55 | -0.53 | 0.35 | 0.24 | 0.19 | 0.52 | 0.51 | 0.52 | 0.4 | 0.39 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2.9 | 3.1 | 3.3 | 3.6 | 4.1 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | 1.3 | -0.2 | -0.8 | -3.2 | -2.1 | -1.8 | -2.6 | -3.0 | 2.8 | 5.4 | 6.3 | 2.3 | 2.8 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.4 | 4.0 | 7.8 | 10.6 | 7.2 | 8.0 | 9.2 | 9.5 | 9.5 | 0 | 0 | 0 | -0.8 | -1.6 | -0.7 |
Nucleus | -0.7 | -4.2 | -4.8 | -7.2 | -7.7 | -7.7 | -7.5 | -8.3 | -8.3 | -1.4 | -4.5 | -5.7 | -2.8 | -2.7 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.9 | 5.8 | 7.9 | 8.7 | 10.7 | 10.3 | 12.4 | 12.4 | 11.3 | -2.5 | -1.8 | -1.9 | -0.1 | -0.5 | -0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.3497 | 5.8472 | 6.1571 | 5.3507 | 5.1294 | 5.7378 | 7.7225 | 7.8126 | 7.5192 | 7.6743 | 7.1854 | 7.3293 | 6.6933 | 5.9546 | 5.8229 | 5.7961 | 5.6561 | 5.7689 |
Actin | 0.0001 | 0 | 0 | 0 | 0.0009 | 0 | 0.0069 | 0 | 0.0116 | 0 | 0.0098 | 0 | 0.0037 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Bud | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0006 | 0 | 0.0014 | 0 | 0.0007 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0013 | 0 | 0.0003 | 0 | 0.0022 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.001 | 0 | 0 | 0 | 0.003 | 0 | 0.0021 | 0 | 0.002 | 0 | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0011 | 0 | 0 | 0 | 0.0113 | 0 | 0.0097 | 0 | 0.003 | 0 | 0.0015 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0.0019 | 0 | 0.0012 | 0 | 0.002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0005 | 0 | 0 | 0 | 0.0044 | 0 | 0.008 | 0 | 0.0036 | 0 | 0.0083 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Golgi | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0017 | 0 | 0.0006 | 0 | 0.0015 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0008 | 0 | 0 | 0 | 0.0015 | 0 | 0.0083 | 0 | 0.0012 | 0 | 0.002 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0.0012 |
Mitochondria | 0.0002 | 0.0001 | 0 | 0 | 0.0012 | 0 | 0.0087 | 0 | 0.0004 | 0 | 0.0091 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0033 |
None | 0.0007 | 0 | 0 | 0 | 0.0102 | 0 | 0.0014 | 0 | 0.0008 | 0 | 0.0028 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0016 | 0.0071 | 0.0003 | 0.0006 | 0.0128 | 0.0001 | 0.0121 | 0.0008 | 0.0041 | 0.0001 | 0.0286 | 0.0003 | 0.0006 | 0.0004 | 0.0013 | 0.0005 | 0.0002 | 0.0001 |
Nucleolus | 0.0253 | 0.0196 | 0.007 | 0.0228 | 0.1733 | 0.0685 | 0.0256 | 0.0088 | 0.0092 | 0.0127 | 0.0821 | 0.0388 | 0.0147 | 0.0084 | 0.0084 | 0.0041 | 0.0831 | 0.039 |
Nucleus | 0.9619 | 0.9725 | 0.9926 | 0.9759 | 0.7224 | 0.9304 | 0.8979 | 0.9902 | 0.9508 | 0.9871 | 0.8282 | 0.9602 | 0.9778 | 0.9909 | 0.9858 | 0.9951 | 0.916 | 0.955 |
Peroxisomes | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0032 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0006 |
Punctate Nuclear | 0.0058 | 0.0005 | 0.0001 | 0.0005 | 0.0557 | 0.0006 | 0.0049 | 0.0001 | 0.0032 | 0.0001 | 0.0109 | 0.0001 | 0.0003 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0002 |
Vacuole | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.002 | 0 | 0.0046 | 0 | 0.0035 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Vacuole Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0 | 0.0019 | 0 | 0.0014 | 0 | 0.0023 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.9319 | 6.6423 | 12.9382 | 16.355 | 6.0211 | 6.4979 | 14.0616 | 13.5985 | 15.6754 | 17.3815 |
Translational Efficiency | 1.3718 | 3.0012 | 1.4994 | 1.2543 | 2.7225 | 2.3999 | 1.3105 | 1.2914 | 1.1228 | 0.9203 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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