Standard name
Human Ortholog
Description Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.07 0.11 0.13 0.14 0.23 0.12 0.12 0.09 0.1 0.05 0.11 0.13 0.13 0.11 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.1 0.14 0.12 0.09 0.28 0.33 0.22 0.6 0.67 0.7 0.52 0.19 0.05 0.06 0 0.05 0.07 0 0 0 0 0 0.05
Nucleus 0.91 0.89 0.87 0.86 0.85 0.72 0.73 0.65 0.69 0.54 0.62 0.71 0.84 0.8 0.76 0.68 0.74 0.68 0.78 0.78 0.77 0.63 0.49 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.16 0.1 0.11 0.15 0.08 0.16 0.26 0.27 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud 0 1 2 1 2 0 4 0 3 6 1 3 0 0 0 0 0 0 0 2 0 1 4 3
Bud Neck 0 0 1 0 2 1 3 2 0 2 0 1 0 0 0 0 0 0 0 0 1 1 3 2
Bud Site 0 0 1 2 1 2 7 5 4 8 9 11 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 2 10 11 9 16 32 45 72 20 19 14 17 8 20 26 30 25 28 0 2 1 0 4 2
Endoplasmic Reticulum 0 2 0 0 0 0 1 0 0 0 0 0 1 0 1 10 10 11 0 0 0 0 1 1
Endosome 1 1 0 1 0 5 3 2 0 0 0 1 0 1 0 9 13 13 1 3 0 0 5 7
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0 2
Mitochondria 15 20 18 15 13 68 104 69 98 105 106 90 29 10 12 9 11 14 0 1 0 2 8 7
Nucleus 80 170 115 107 122 177 231 201 111 85 94 122 128 147 150 153 170 142 87 168 112 59 93 61
Nuclear Periphery 0 0 0 0 0 2 0 0 0 1 1 0 0 2 0 1 0 1 0 0 0 0 0 0
Nucleolus 2 0 0 2 0 4 4 4 3 4 5 7 1 1 5 1 1 1 2 6 1 1 8 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 5 6 4 2 6 14 1 1 5 6 0 0 1 7 11 8 1 5 4 1 4 4
Vac/Vac Membrane 4 16 2 1 1 6 3 6 1 1 2 2 6 11 13 35 22 23 16 18 22 24 51 46
Unique Cell Count 88 192 132 124 144 245 316 311 162 156 152 172 152 183 197 225 231 208 112 216 147 95 192 161
Labelled Cell Count 105 222 155 144 161 299 411 375 241 232 237 260 173 192 208 257 264 244 112 216 147 95 192 161


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.0 4.8 4.8 5.4 4.5 4.7 5.4 3.9 3.9 3.6 3.8 4.7 4.6 4.8 6.9 6.9 6.7 4.8 4.6 4.9
Std Deviation (1e-4) 0.8 1.0 1.0 1.3 1.5 1.3 1.8 2.2 1.5 1.4 1.3 1.1 1.2 1.2 1.1 2.0 1.8 1.5 1.2 1.3 1.4
Intensity Change (Log2) – – – 0.01 0.18 -0.08 -0.02 0.17 -0.29 -0.3 -0.4 -0.32 -0.02 -0.06 -0.01 0.52 0.53 0.49 -0.01 -0.06 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 0.8 1.4 1.7 3.7 1.1 1.1 0.3 0.5 -1.0 0.8 1.4 1.4 0.8 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.2 0 0 2.1 0 0 0 0 0 0 0 -3.3 -3.0 -2.1
Nucleus -0.2 -0.6 -3.3 -3.2 -4.8 -3.8 -6.0 -4.8 -3.4 -0.7 -1.6 -2.5 -4.0 -3.0 -3.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.3 0 0 0 0 0 0 0 0 0.4 0
Vacuole 0 0 0 0 0 0 0 0 0 0 2.0 2.2 4.2 3.0 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1777 2.9868 2.2385 2.2787 1.9154 2.4655 0.4251 0.4035 0.4158 -0.0111 0.7218 0.1828 0.1233 -0.047 -0.1494 -0.2786 -0.5028 -0.0489
Actin 0.0139 0.0001 0.0023 0.0471 0.0013 0.0058 0.0196 0 0.0141 0.0009 0.0293 0.0016 0.0175 0.0082 0.0208 0.0003 0.0019 0.0089
Bud 0.0002 0 0.0001 0.0001 0.0002 0 0.0008 0 0.0005 0.0003 0.0009 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0004
Bud Neck 0.003 0.0007 0.0027 0.0021 0.0225 0.0029 0.0011 0.0003 0.0016 0.001 0.0018 0.0017 0.0014 0.0006 0.0022 0.0001 0.0008 0.0036
Bud Periphery 0.0007 0 0.0002 0.0002 0.0004 0.0001 0.0018 0 0.0007 0.0006 0.0035 0.0006 0.0001 0.0002 0.0002 0 0.0001 0.0003
Bud Site 0.0024 0.0004 0.0009 0.0004 0.0011 0.0001 0.0062 0.0001 0.0041 0.0008 0.0029 0.0003 0.0003 0.0012 0.0009 0 0.0001 0.0002
Cell Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0028 0.0194 0.0079 0.004 0.0029 0.0009 0.0048 0.005 0.017 0.0066 0.002 0.008 0.0091 0.0017 0.0028 0.0055 0.0049 0.0053
Cytoplasmic Foci 0.0131 0.0004 0.0025 0.0005 0.0023 0.0011 0.0137 0.0001 0.0054 0.0023 0.0293 0.0035 0.0059 0.0002 0.0018 0.0001 0.0003 0.0005
Eisosomes 0.0013 0 0.0001 0.0007 0 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0001 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0009 0.0101 0.0005 0.0005 0.0002 0.0001 0.0025 0.0011 0.004 0.0079 0.0019 0.0015 0.0023 0.0003 0.0008 0.0005 0.0006 0.0009
Endosome 0.0098 0.0018 0.0043 0.0008 0.0057 0.001 0.0187 0.0005 0.0166 0.0319 0.024 0.0092 0.006 0.0006 0.0032 0.0003 0.0022 0.0027
Golgi 0.0043 0 0.002 0.0012 0.0052 0.0008 0.0083 0 0.0061 0.0059 0.0404 0.0034 0.0025 0.0005 0.0017 0 0.0001 0.0019
Lipid Particles 0.0138 0.0002 0.0033 0.0009 0.0097 0.0065 0.0142 0 0.0037 0.0224 0.0321 0.0064 0.0123 0.0005 0.009 0.0001 0.0001 0.0023
Mitochondria 0.005 0.0003 0.0094 0.0003 0.0277 0.0004 0.0079 0.0002 0.0127 0.038 0.0232 0.0053 0.0013 0.0005 0.0009 0.0002 0.0009 0.001
None 0.0255 0.0171 0.0268 0.0581 0.002 0.0063 0.0032 0.0006 0.0111 0.0005 0.0012 0.0017 0.0136 0.0074 0.0102 0.0326 0.0043 0.0114
Nuclear Periphery 0.0098 0.0215 0.0061 0.0023 0.0131 0.0018 0.0229 0.0135 0.0258 0.0269 0.0242 0.0059 0.0224 0.0067 0.0125 0.0089 0.0358 0.0083
Nucleolus 0.0202 0.0326 0.0279 0.036 0.0361 0.0456 0.0167 0.0154 0.0126 0.006 0.0213 0.033 0.0159 0.0207 0.0163 0.004 0.0447 0.0287
Nucleus 0.8377 0.8792 0.874 0.8323 0.849 0.9042 0.8308 0.9579 0.852 0.8289 0.7088 0.9049 0.8529 0.9285 0.9066 0.9443 0.8495 0.8969
Peroxisomes 0.0119 0 0.0027 0.0008 0.0031 0.0037 0.0098 0 0.0013 0.0011 0.0315 0.0031 0.0103 0.0002 0.0014 0 0.0001 0.0002
Punctate Nuclear 0.021 0.0143 0.0248 0.0111 0.0162 0.0184 0.0119 0.0045 0.0081 0.0014 0.0109 0.0085 0.0248 0.0212 0.0071 0.0029 0.0526 0.0257
Vacuole 0.0017 0.0013 0.0009 0.0003 0.0009 0.0001 0.0026 0.0005 0.0016 0.012 0.0055 0.0007 0.0006 0.0002 0.0006 0.0001 0.0003 0.0003
Vacuole Periphery 0.0008 0.0005 0.0003 0.0001 0.0004 0.0001 0.0021 0.0001 0.0011 0.0043 0.0044 0.0005 0.0005 0.0001 0.0004 0.0001 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4105 13.7165 8.6814 11.8592 15.2131 8.1187 13.3588 12.9394 10.7781 17.0349
Translational Efficiency 0.7946 0.8633 0.8613 0.5505 0.645 0.9402 0.6382 0.6423 0.7266 0.5873

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ecm22

Ecm22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available