| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
| Bud | 0 | 1 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 6 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 1 |
| Bud Neck | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
| Bud Site | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 2 | 8 | 7 | – | – | – | – | – | – | – | – | – | – | – | – | 24 | 26 | 12 | 1 | 0 | 3 | 1 | 0 | 0 |
| Endoplasmic Reticulum | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 7 | 3 | 4 | 0 | 0 | 3 | 0 | 0 | 0 |
| Endosome | 3 | 12 | 18 | – | – | – | – | – | – | – | – | – | – | – | – | 63 | 46 | 24 | 33 | 44 | 48 | 11 | 51 | 47 |
| Golgi | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 |
| Mitochondria | 4 | 17 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 8 | 16 | 6 | 1 | 2 | 0 | 0 | 3 | 4 |
| Nucleus | 1 | 2 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
| Nucleolus | 8 | 9 | 6 | – | – | – | – | – | – | – | – | – | – | – | – | 11 | 13 | 3 | 0 | 1 | 0 | 3 | 1 | 0 |
| Peroxisomes | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 4 | 7 | 20 | – | – | – | – | – | – | – | – | – | – | – | – | 55 | 26 | 18 | 6 | 13 | 11 | 1 | 4 | 1 |
| Vac/Vac Membrane | 5 | 11 | 8 | – | – | – | – | – | – | – | – | – | – | – | – | 15 | 8 | 7 | 6 | 14 | 7 | 1 | 9 | 8 |
| Unique Cell Count | 19 | 55 | 43 | 128 | 92 | 53 | 58 | 92 | 84 | 26 | 75 | 68 | ||||||||||||
| Labelled Cell Count | 27 | 67 | 62 | 199 | 150 | 77 | 58 | 92 | 84 | 26 | 75 | 68 | ||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 5.4 | 5.9 | 6.6 | – | – | – | – | – | – | – | – | – | – | – | – | 6.3 | 7.0 | 7.3 | – | 6.1 | 7.6 |
| Std Deviation (1e-4) | 0.9 | 1.2 | 2.3 | – | – | – | – | – | – | – | – | – | – | – | – | 1.5 | 1.7 | 1.6 | – | 0.8 | 2.0 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | -0.05 | 0.08 | 0.14 | – | -0.12 | 0.21 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0.4 | 1.5 | 0.8 |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0.8 | 0.9 | 0.3 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 2.5 | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | -0.4 | -2.1 | -1.3 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -1.1 | -1.7 | -0.7 |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 13.0158 | 7.5067 | 12.8116 | 15.1828 | 7.349 | 8.3926 | 10.9106 | 17.2892 | 15.8436 | 14.0406 |
| Translational Efficiency | 0.4053 | 0.6007 | 0.4145 | 0.4503 | 0.526 | 0.7333 | 0.5832 | 0.29 | 0.4386 | 0.4668 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery |
| Localization | |
| Cell Percentages | — |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Vps34
Vps34
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Vps34-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
