Standard name | |
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Human Ortholog | |
Description | E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 5 | 1 | 1 | 2 |
Bud | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | – | 4 | 6 | 0 | 0 | 4 | – | – | – | 2 | 0 | 1 | 1 | 3 | 9 |
Bud Neck | 0 | 0 | 3 | 2 | 0 | 0 | 3 | 2 | 0 | – | 2 | 2 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 2 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | – | 0 | 1 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 134 | 147 | 170 | 102 | 208 | 236 | 308 | 320 | 195 | – | 111 | 167 | 94 | 193 | 291 | – | – | – | 102 | 130 | 92 | 109 | 169 | 181 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 2 | 1 | 1 | – | – | – | 2 | 1 | 2 | 1 | 0 | 4 |
Endosome | 10 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 7 | 10 | 0 | – | – | – | 13 | 4 | 3 | 3 | 1 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 11 | 33 | 18 | 55 | 58 | 69 | 122 | 129 | 90 | – | 73 | 130 | 16 | 9 | 23 | – | – | – | 32 | 23 | 36 | 5 | 10 | 17 |
Nucleus | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | – | 1 | 1 | 11 | 12 | 1 | – | – | – | 6 | 5 | 2 | 6 | 7 | 6 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | – | 4 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 6 | 6 | – | 2 | 6 | 0 | 0 | 0 | – | – | – | 9 | 3 | 2 | 6 | 12 | 14 |
Vac/Vac Membrane | 25 | 11 | 0 | 3 | 11 | 5 | 2 | 3 | 2 | – | 0 | 2 | 47 | 28 | 3 | – | – | – | 28 | 21 | 10 | 23 | 29 | 39 |
Unique Cell Count | 168 | 177 | 181 | 140 | 255 | 270 | 351 | 397 | 246 | 154 | 257 | 162 | 239 | 306 | 215 | 204 | 169 | 169 | 249 | 297 | ||||
Labelled Cell Count | 183 | 195 | 195 | 167 | 283 | 314 | 440 | 468 | 302 | 197 | 317 | 177 | 254 | 324 | 215 | 204 | 169 | 169 | 249 | 297 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 3.5 | 3.6 | 3.2 | 3.3 | 3.4 | 2.7 | 2.9 | 2.6 | – | 2.8 | 2.2 | 4.1 | 4.4 | 3.7 | – | – | – | 4.4 | 4.1 | 4.5 |
Std Deviation (1e-4) | 0.9 | 1.2 | 2.2 | 1.5 | 1.2 | 2.4 | 1.3 | 1.5 | 1.3 | – | 1.5 | 1.4 | 1.3 | 1.4 | 1.2 | – | – | – | 1.5 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.13 | -0.09 | -0.39 | -0.31 | -0.48 | – | -0.39 | -0.69 | 0.2 | 0.27 | 0.05 | – | – | – | 0.28 | 0.2 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0569 | 0.0088 | 0.0397 | 0.0604 | 0.0729 | 0.0031 | 0.0148 | 0.0003 | 0.0072 | 0.002 | 0.0038 | 0.0009 | – | – | – | – | – | – |
Bud | 0.0013 | 0.0005 | 0.0006 | 0.0019 | 0.0027 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | – | – | – | – | – | – |
Bud Neck | 0.0062 | 0.0043 | 0.0023 | 0.0263 | 0.0042 | 0.0059 | 0.001 | 0.0004 | 0.0015 | 0.0014 | 0.0009 | 0.0036 | – | – | – | – | – | – |
Bud Periphery | 0.0045 | 0.0007 | 0.0009 | 0.0113 | 0.005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | – | – | – | – | – | – |
Bud Site | 0.0046 | 0.0167 | 0.0112 | 0.03 | 0.0222 | 0.0004 | 0.0011 | 0.0014 | 0.0046 | 0.0005 | 0.0004 | 0.0002 | – | – | – | – | – | – |
Cell Periphery | 0.001 | 0.0008 | 0.0003 | 0.0018 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.2149 | 0.2736 | 0.2543 | 0.2617 | 0.2624 | 0.3249 | 0.2167 | 0.3306 | 0.308 | 0.4362 | 0.2977 | 0.3196 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0303 | 0.0096 | 0.016 | 0.012 | 0.0082 | 0.0162 | 0.0768 | 0.0163 | 0.0153 | 0.0147 | 0.0123 | 0.0144 | – | – | – | – | – | – |
Eisosomes | 0.0018 | 0.0003 | 0.0005 | 0.0007 | 0.0022 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0133 | 0.0058 | 0.005 | 0.015 | 0.0054 | 0.0041 | 0.0072 | 0.0036 | 0.0022 | 0.0044 | 0.0033 | 0.004 | – | – | – | – | – | – |
Endosome | 0.0307 | 0.0018 | 0.0084 | 0.0193 | 0.0133 | 0.008 | 0.0446 | 0.0024 | 0.0072 | 0.0309 | 0.0198 | 0.0116 | – | – | – | – | – | – |
Golgi | 0.0064 | 0.0007 | 0.0062 | 0.0074 | 0.0121 | 0.0009 | 0.0132 | 0.0001 | 0.003 | 0.0111 | 0.0193 | 0.0036 | – | – | – | – | – | – |
Lipid Particles | 0.0198 | 0.0024 | 0.0072 | 0.0077 | 0.0181 | 0.006 | 0.0688 | 0.0002 | 0.0017 | 0.0005 | 0.0046 | 0.0121 | – | – | – | – | – | – |
Mitochondria | 0.0111 | 0.0027 | 0.0118 | 0.0655 | 0.0235 | 0.0083 | 0.0087 | 0.001 | 0.0008 | 0.002 | 0.0689 | 0.0009 | – | – | – | – | – | – |
None | 0.4511 | 0.5886 | 0.4958 | 0.3142 | 0.5159 | 0.3923 | 0.4082 | 0.4407 | 0.4277 | 0.3428 | 0.398 | 0.3739 | – | – | – | – | – | – |
Nuclear Periphery | 0.0285 | 0.0027 | 0.0074 | 0.017 | 0.0028 | 0.0086 | 0.0227 | 0.0075 | 0.0047 | 0.0113 | 0.0064 | 0.0064 | – | – | – | – | – | – |
Nucleolus | 0.0035 | 0.0013 | 0.0022 | 0.0025 | 0.0008 | 0.0025 | 0.0012 | 0.0015 | 0.0008 | 0.0009 | 0.0008 | 0.0016 | – | – | – | – | – | – |
Nucleus | 0.0529 | 0.0641 | 0.0886 | 0.1128 | 0.0106 | 0.1866 | 0.0713 | 0.1693 | 0.1939 | 0.1249 | 0.1324 | 0.2108 | – | – | – | – | – | – |
Peroxisomes | 0.0109 | 0.0038 | 0.0049 | 0.0095 | 0.0046 | 0.002 | 0.0192 | 0.0002 | 0.0093 | 0.0009 | 0.0036 | 0.0015 | – | – | – | – | – | – |
Punctate Nuclear | 0.033 | 0.0092 | 0.0339 | 0.008 | 0.0025 | 0.025 | 0.0205 | 0.0219 | 0.0103 | 0.0117 | 0.0226 | 0.0325 | – | – | – | – | – | – |
Vacuole | 0.0106 | 0.0016 | 0.0024 | 0.0107 | 0.0077 | 0.0037 | 0.0025 | 0.0021 | 0.0013 | 0.0023 | 0.0035 | 0.0017 | – | – | – | – | – | – |
Vacuole Periphery | 0.0066 | 0.0001 | 0.0006 | 0.0041 | 0.0015 | 0.0008 | 0.0011 | 0.0002 | 0.0002 | 0.0009 | 0.0011 | 0.0003 | – | – | – | – | – | – |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.4176 | 9.2915 | 17.8635 | 24.3743 | 13.4761 | 15.11 | 23.6467 | 27.1643 | 25.9895 | 20.2198 |
Translational Efficiency | 0.3052 | 0.5893 | 0.3764 | 0.3096 | 0.3064 | 0.3554 | 0.241 | 0.2509 | 0.2791 | 0.2845 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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