Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.14 0.2 0.28 0.31 0.09 0.13 – 0.14 0.12 0.11 0.09 0.1 0.11 0 0.08 0.07 0.15 0 0 0 0.12 0.1 0.06
Bud 0 0 0 0 0 0.12 0.05 – 0.08 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.14 0.1 0.19 0.1 0.05 0.06 – 0 0 0 0 0.05 0 0 0.1 0.11 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.14 0.06 0.11 0.05 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.27 0.09 0.27 0.19 0.21 0.14 0.16 – 0.09 0.11 0.09 0.12 0.12 0.13 0.18 0.14 0.13 0.09 0.16 0.27 0.28 0.13 0.17 0.16
Golgi 0.28 0.41 0.27 0.37 0.49 0.17 0.55 – 0.28 0.4 0.33 0.23 0.76 0.68 0.58 0.31 0.4 0.46 0.71 0.43 0.39 0.58 0.53 0.47
Mitochondria 0.49 0.15 0.13 0.09 0.05 0.58 0.23 – 0.67 0.49 0.64 0.64 0.33 0.32 0.26 0.05 0.09 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 – 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0.05 0.09 0 0.06 0 0.06 0.15 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.1 0 0.11 – 0 0.08 0 0.08 0 0 0 0 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.24 0.31 0.06 0.08 0.08 0 – 0 0 0 0 0 0 0 0.54 0.44 0.35 0.06 0.17 0.2 0.06 0.09 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 38 33 27 61 16 35 – 45 37 28 28 22 49 17 17 12 19 9 14 6 30 52 26
Bud 5 2 1 1 6 21 14 – 27 21 19 18 5 10 9 2 4 1 0 1 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 4 0 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 2 1 3 1 – 3 3 5 2 0 2 1 1 0 0 0 0 0 0 0 0
Cytoplasm 8 40 16 19 19 9 15 – 8 11 8 8 10 12 16 21 18 16 0 4 0 0 4 2
Endoplasmic Reticulum 5 39 10 11 10 3 1 – 5 4 4 4 10 4 4 5 4 3 1 7 1 6 19 15
Endosome 92 25 44 19 42 24 43 – 30 34 23 38 26 60 71 31 22 11 60 95 59 32 88 69
Golgi 95 113 43 36 97 29 149 – 88 123 84 73 169 307 225 68 65 59 272 149 84 145 273 196
Mitochondria 165 41 21 9 9 99 63 – 213 150 162 202 73 144 101 11 14 13 3 7 8 5 11 6
Nucleus 0 0 0 1 0 0 5 – 0 1 0 1 0 0 0 3 0 2 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 1 1 1 – 0 3 1 5 1 5 0 0 0 1 2 2 0 0 1 4
Nucleolus 9 4 2 1 2 9 7 – 14 9 7 24 3 4 4 4 3 5 0 0 0 0 0 0
Peroxisomes 13 1 0 0 2 6 9 – 17 26 9 18 9 27 59 0 0 1 7 2 7 9 9 4
SpindlePole 8 1 8 3 19 4 31 – 12 24 11 25 0 7 8 8 13 3 0 0 1 0 0 1
Vac/Vac Membrane 62 67 51 6 16 14 7 – 4 5 2 6 9 18 7 119 72 44 23 60 42 14 46 87
Unique Cell Count 336 276 162 98 199 172 272 319 304 252 318 222 450 387 220 164 127 383 351 215 248 514 422
Labelled Cell Count 475 371 231 135 285 238 381 467 455 363 456 337 649 522 290 227 178 383 351 215 248 514 422


Early Golgi, Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 11.5 9.9 10.7 11.7 8.2 11.3 – 10.6 11.0 11.3 10.9 12.6 12.8 13.0 13.8 14.5 14.4 11.2 11.7 11.2
Std Deviation (1e-4) 2.4 2.1 1.9 1.9 2.1 2.2 2.2 – 2.2 2.5 2.6 2.7 2.2 2.8 3.2 2.9 3.5 3.1 3.6 3.6 3.0
Intensity Change (Log2) – – – 0.12 0.25 -0.27 0.2 – 0.1 0.16 0.2 0.14 0.35 0.37 0.39 0.48 0.56 0.54 0.18 0.24 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 2.2 -2.9 -2.1 – -1.8 -2.4 -2.6 -3.6 -2.9 -3.0 -5.9 -3.6 -3.4 -1.2
Bud 0 0 4.3 2.5 – 3.5 3.0 3.2 2.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 -0.1 -1.6 -1.7 – -3.5 -2.8 -2.8 -3.5 -2.1 -3.8 -2.6 -0.1 0.3 0.7
Endoplasmic Reticulum 1.4 -0.5 -2.1 0 – -2.7 0 -2.5 0 -0.7 0 0 -1.9 -1.7 -1.6
Endosome -1.4 -1.3 -3.0 -2.9 – -5.1 -4.4 -4.9 -4.2 -3.9 -4.0 -2.3 -3.2 -3.1 -4.0
Golgi 1.7 4.3 -2.2 5.7 – 0.2 3.0 1.5 -0.9 9.6 9.2 6.8 0.9 2.5 3.5
Mitochondria -0.9 -2.9 8.5 2.6 – 11.2 7.8 10.3 10.5 4.5 4.7 3.4 -2.8 -1.3 -0.7
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.0 0 – 1.8 0 0 2.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 3.0 3.8 0 3.1 0 3.2 5.3 0 0 0
SpindlePole 0 1.7 -1.3 2.3 – -0.6 1.2 -0.3 1.2 0 0 0 -0.6 1.1 0
Vacuole -4.8 -5.7 -5.4 -8.6 – -9.8 -9.4 -9.1 -9.5 -7.3 -9.5 -10.3 4.4 2.3 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.9709 11.4166 9.9118 9.5172 10.2904 10.9361 9.9355 13.2107 11.335 11.0009 9.1289 12.0075 16.2497 17.4466 16.8813 15.6009 16.0538 16.9012
Actin 0.0406 0.0014 0.0095 0.0142 0.0003 0.0022 0.0575 0.007 0.0034 0.0099 0.0491 0.0096 0.0101 0.0093 0.0082 0.0104 0.0011 0.0101
Bud 0.0003 0 0.0001 0.0001 0 0 0.0005 0.0001 0.0004 0.0001 0.0043 0.0006 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0006 0.0001 0.0003 0.0006 0 0.0001 0.0006 0.0002 0.0006 0.0002 0.0008 0.0005 0.0003 0.0001 0.0002 0.0002 0.0001 0.0005
Bud Periphery 0.0005 0 0.0001 0.0001 0 0 0.0005 0.0001 0.0005 0.0001 0.0032 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001
Bud Site 0.0014 0 0.0003 0.0004 0 0 0.0022 0.0001 0.0057 0.0004 0.0023 0.0003 0.0002 0.0002 0.0001 0.0001 0 0
Cell Periphery 0.0002 0 0 0.0001 0 0 0.0003 0.0001 0.0004 0 0.0006 0.0001 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0034 0.0006 0.0032 0.0004 0.0014 0.0006 0.0054 0.002 0.002 0.0014 0.0022 0.0015 0.0008 0.0017 0.0007 0.0009 0.0004 0.0007
Cytoplasmic Foci 0.0456 0.0389 0.0336 0.0298 0.0213 0.0199 0.0704 0.058 0.0527 0.0446 0.0662 0.0401 0.048 0.0459 0.0353 0.0529 0.029 0.0276
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0002 0 0 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0.0004 0.0007 0.0005 0.0006 0.0005 0.0087 0.0015 0.0012 0.0007 0.0013 0.0007 0.0007 0.0007 0.0003 0.0003 0.0023 0.0006
Endosome 0.1053 0.1602 0.1504 0.2201 0.1494 0.1541 0.169 0.2213 0.1661 0.2759 0.1733 0.1486 0.1558 0.2468 0.1446 0.2774 0.1544 0.1374
Golgi 0.7104 0.7746 0.7623 0.6426 0.7836 0.7961 0.5733 0.6687 0.7246 0.5979 0.52 0.7557 0.7405 0.6654 0.7968 0.6304 0.7554 0.8109
Lipid Particles 0.03 0.008 0.0035 0.0072 0.0014 0.0026 0.0221 0.0103 0.0071 0.0049 0.0841 0.0072 0.0107 0.0087 0.0033 0.0017 0.0026 0.0027
Mitochondria 0.027 0.001 0.0241 0.0082 0.0014 0.0027 0.0379 0.0049 0.0175 0.0064 0.0619 0.0251 0.0139 0.0022 0.0042 0.0014 0.0015 0.003
None 0.0036 0 0.001 0 0 0.0083 0.0026 0 0.0001 0.0001 0.0009 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0091 0.0005 0.0003 0.0004 0.0192 0.0004 0.0046 0.0008 0.0004 0.0005 0.0033 0.0002 0.0004 0.0002 0.0001 0.0001 0.0018 0.0001
Nucleolus 0.0014 0 0 0.0001 0 0.0004 0.0045 0 0.0002 0 0.0013 0.0001 0 0 0 0 0 0
Nucleus 0.0025 0 0.0001 0.0001 0.0002 0.0007 0.0061 0.0001 0.0002 0.0001 0.0007 0.0001 0.0001 0 0 0 0.0001 0
Peroxisomes 0.0064 0.0004 0.0033 0.0205 0.0001 0.0002 0.0173 0.0008 0.0033 0.0072 0.0133 0.0034 0.0048 0.002 0.0013 0.0074 0.0002 0.0005
Punctate Nuclear 0.0027 0 0.0012 0.0002 0.0001 0.0024 0.0073 0 0.0001 0.0002 0.0018 0.0002 0.0001 0 0 0 0 0
Vacuole 0.0024 0.007 0.0016 0.0206 0.0052 0.0028 0.004 0.011 0.0047 0.0185 0.004 0.0026 0.0057 0.0071 0.0015 0.0083 0.0291 0.0021
Vacuole Periphery 0.0039 0.0069 0.0042 0.0338 0.0157 0.0058 0.0048 0.0133 0.0088 0.0308 0.0052 0.0031 0.0074 0.0093 0.0032 0.0083 0.0219 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.1266 71.3549 69.5597 70.0246 57.8333 68.8268 65.4273 72.0866 90.209 67.8155
Translational Efficiency 1.9112 1.3967 1.7354 2.0418 1.5921 1.9658 1.6265 1.6608 1.4635 1.581

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
Localization
Cell Percentages Golgi (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ssp120

Ssp120


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssp120-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available