Standard name | |
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Human Ortholog | |
Description | Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 1 | 6 | 6 |
Bud | 0 | 1 | 0 | 2 | 3 | 2 | 3 | 2 | 6 | – | 7 | 6 | 1 | 1 | 0 | 2 | 0 | 3 | 3 | 3 | 2 | 1 | 8 | 11 |
Bud Neck | 2 | 0 | 39 | 34 | 37 | 18 | 42 | 29 | 16 | – | 7 | 20 | 0 | 0 | 0 | 5 | 2 | 6 | 1 | 2 | 3 | 1 | 4 | 12 |
Bud Site | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 0 | 1 | 1 | 3 | 0 | 3 | – | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 2 |
Cytoplasm | 85 | 66 | 275 | 262 | 479 | 503 | 767 | 638 | 415 | – | 348 | 504 | 112 | 184 | 282 | 427 | 369 | 354 | 88 | 59 | 172 | 58 | 143 | 160 |
Endoplasmic Reticulum | 6 | 0 | 7 | 2 | 1 | 1 | 2 | 0 | 0 | – | 0 | 0 | 2 | 3 | 0 | 62 | 38 | 46 | 5 | 0 | 4 | 3 | 7 | 12 |
Endosome | 1 | 5 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | – | 0 | 1 | 19 | 31 | 74 | 75 | 61 | 50 | 20 | 15 | 9 | 6 | 19 | 12 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 2 | 3 |
Mitochondria | 74 | 43 | 2 | 13 | 6 | 50 | 81 | 39 | 128 | – | 131 | 101 | 46 | 16 | 17 | 44 | 27 | 33 | 35 | 19 | 66 | 20 | 98 | 77 |
Nucleus | 22 | 16 | 19 | 10 | 4 | 2 | 4 | 11 | 5 | – | 7 | 4 | 30 | 30 | 29 | 41 | 46 | 40 | 32 | 31 | 22 | 6 | 12 | 11 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | – | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 2 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 5 | 1 | 6 | 13 | 6 | 10 | 7 | 10 | 6 | – | 10 | 15 | 4 | 3 | 5 | 13 | 13 | 9 | 31 | 23 | 25 | 16 | 30 | 30 |
Vac/Vac Membrane | 15 | 33 | 1 | 3 | 11 | 8 | 2 | 1 | 1 | – | 0 | 2 | 52 | 85 | 113 | 225 | 124 | 131 | 55 | 51 | 83 | 40 | 138 | 117 |
Unique Cell Count | 185 | 143 | 343 | 318 | 526 | 554 | 831 | 685 | 486 | 443 | 595 | 246 | 322 | 472 | 738 | 587 | 562 | 303 | 223 | 445 | 168 | 496 | 482 | |
Labelled Cell Count | 211 | 167 | 358 | 342 | 554 | 595 | 913 | 734 | 583 | 516 | 656 | 267 | 355 | 523 | 896 | 681 | 677 | 303 | 223 | 445 | 168 | 496 | 482 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.8 | 4.5 | 4.2 | 4.8 | 3.5 | 3.6 | 4.1 | 3.2 | – | 3.2 | 3.5 | 4.0 | 4.2 | 4.2 | 5.8 | 5.8 | 5.6 | 3.9 | 3.8 | 4.3 |
Std Deviation (1e-4) | 0.5 | 0.6 | 0.5 | 1.0 | 1.1 | 1.0 | 0.9 | 1.1 | 1.0 | – | 1.5 | 0.8 | 0.8 | 1.1 | 0.9 | 1.0 | 1.1 | 1.1 | 1.1 | 1.1 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.08 | -0.36 | -0.32 | -0.15 | -0.5 | – | -0.5 | -0.38 | -0.19 | -0.1 | -0.12 | 0.35 | 0.36 | 0.31 | -0.23 | -0.26 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 11.4 | 9.0 | 9.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.5875 | -1.3428 | -1.5177 | -1.8163 | -1.6851 | -1.5394 | -0.9066 | -0.6649 | -0.7549 | -0.8861 | -1.0856 | -0.8478 | -1.5163 | -1.2558 | -1.2799 | -0.7809 | -1.1643 | -1.2989 |
Actin | 0.0104 | 0.0003 | 0.011 | 0.0006 | 0.0193 | 0.0039 | 0.0141 | 0.0003 | 0.0118 | 0.0011 | 0.0034 | 0.0056 | 0.0289 | 0.0002 | 0.0025 | 0.0203 | 0.0289 | 0.0043 |
Bud | 0.0036 | 0 | 0.005 | 0 | 0.0002 | 0.0025 | 0.0006 | 0.0005 | 0.0006 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0008 | 0.0022 | 0.0014 | 0.0007 | 0.0001 |
Bud Neck | 0.0028 | 0.0003 | 0.0011 | 0.0015 | 0.0013 | 0.0032 | 0.0017 | 0.0009 | 0.0005 | 0.0012 | 0.0016 | 0.0049 | 0.0013 | 0.0003 | 0.0005 | 0.0008 | 0.0015 | 0.0016 |
Bud Periphery | 0.0029 | 0 | 0.0017 | 0 | 0.0005 | 0.0013 | 0.0008 | 0.0006 | 0.0015 | 0.0002 | 0.0003 | 0.0031 | 0.0004 | 0.0005 | 0.0033 | 0.0017 | 0.0012 | 0.0001 |
Bud Site | 0.006 | 0.0005 | 0.0055 | 0.0003 | 0.0003 | 0.0002 | 0.0028 | 0.0036 | 0.0019 | 0.0005 | 0.001 | 0.0005 | 0.0018 | 0.0024 | 0.0052 | 0.0067 | 0.0026 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.1194 | 0.1582 | 0.1226 | 0.1659 | 0.1025 | 0.1858 | 0.123 | 0.1553 | 0.1554 | 0.1818 | 0.2084 | 0.2055 | 0.1338 | 0.1826 | 0.118 | 0.1454 | 0.2003 | 0.2097 |
Cytoplasmic Foci | 0.0468 | 0.0149 | 0.0169 | 0.0161 | 0.0217 | 0.035 | 0.0454 | 0.0236 | 0.0194 | 0.057 | 0.0303 | 0.0294 | 0.0255 | 0.018 | 0.0165 | 0.019 | 0.0119 | 0.0138 |
Eisosomes | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0069 | 0.0057 | 0.0042 | 0.0044 | 0.0023 | 0.0035 | 0.007 | 0.0065 | 0.0036 | 0.0094 | 0.0029 | 0.0032 | 0.0044 | 0.0041 | 0.0029 | 0.0022 | 0.0045 | 0.0032 |
Endosome | 0.0244 | 0.0102 | 0.0113 | 0.0106 | 0.0117 | 0.0097 | 0.0286 | 0.0088 | 0.014 | 0.0122 | 0.0191 | 0.014 | 0.016 | 0.0076 | 0.0108 | 0.0073 | 0.0162 | 0.0094 |
Golgi | 0.004 | 0.0003 | 0.0013 | 0.0003 | 0.0049 | 0.0014 | 0.0068 | 0.0007 | 0.0047 | 0.003 | 0.0038 | 0.0016 | 0.003 | 0.0002 | 0.0009 | 0.0026 | 0.0024 | 0.001 |
Lipid Particles | 0.0194 | 0.0065 | 0.0046 | 0.0077 | 0.0065 | 0.0045 | 0.0105 | 0.0038 | 0.0024 | 0.0022 | 0.0086 | 0.0024 | 0.0069 | 0.0021 | 0.0043 | 0.0024 | 0.0032 | 0.0038 |
Mitochondria | 0.0094 | 0.0004 | 0.007 | 0.0008 | 0.002 | 0.0009 | 0.0021 | 0.0008 | 0.0064 | 0.0013 | 0.0236 | 0.001 | 0.0015 | 0.0006 | 0.0015 | 0.0021 | 0.0019 | 0.0008 |
None | 0.5197 | 0.6247 | 0.5655 | 0.4877 | 0.5667 | 0.4702 | 0.5831 | 0.6504 | 0.6117 | 0.5065 | 0.5278 | 0.4878 | 0.5726 | 0.6219 | 0.6235 | 0.5235 | 0.4451 | 0.5372 |
Nuclear Periphery | 0.0229 | 0.0187 | 0.0144 | 0.0186 | 0.0245 | 0.0143 | 0.0114 | 0.011 | 0.0118 | 0.0236 | 0.0087 | 0.0087 | 0.0148 | 0.0267 | 0.0156 | 0.0136 | 0.0195 | 0.0132 |
Nucleolus | 0.0073 | 0.0035 | 0.0064 | 0.0056 | 0.0057 | 0.0059 | 0.0036 | 0.0031 | 0.0023 | 0.0022 | 0.0028 | 0.0051 | 0.0028 | 0.0037 | 0.0038 | 0.0041 | 0.0053 | 0.0037 |
Nucleus | 0.1118 | 0.1098 | 0.1456 | 0.1625 | 0.1302 | 0.1674 | 0.0811 | 0.1044 | 0.094 | 0.1016 | 0.0991 | 0.1434 | 0.1345 | 0.0918 | 0.1413 | 0.1618 | 0.1914 | 0.1304 |
Peroxisomes | 0.0097 | 0.0004 | 0.0074 | 0.0013 | 0.0009 | 0.001 | 0.0117 | 0.0008 | 0.0118 | 0.0122 | 0.0019 | 0.0037 | 0.0018 | 0.0004 | 0.0023 | 0.003 | 0.0018 | 0.0008 |
Punctate Nuclear | 0.0666 | 0.0418 | 0.0647 | 0.1128 | 0.0968 | 0.0871 | 0.0608 | 0.0204 | 0.0423 | 0.0804 | 0.0515 | 0.0769 | 0.046 | 0.0325 | 0.0412 | 0.0801 | 0.0552 | 0.0649 |
Vacuole | 0.004 | 0.0031 | 0.003 | 0.0024 | 0.001 | 0.0017 | 0.0036 | 0.0036 | 0.0028 | 0.0025 | 0.003 | 0.0019 | 0.0024 | 0.0026 | 0.0027 | 0.0012 | 0.0052 | 0.0013 |
Vacuole Periphery | 0.0011 | 0.0005 | 0.0006 | 0.0003 | 0.0008 | 0.0004 | 0.0009 | 0.0006 | 0.0007 | 0.0009 | 0.0013 | 0.0004 | 0.0006 | 0.0005 | 0.0008 | 0.0004 | 0.0009 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.5039 | 11.7091 | 12.5117 | 15.8158 | 8.8309 | 15.1857 | 16.5747 | 20.2961 | 17.9354 | 18.1057 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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