Standard name
Human Ortholog
Description Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.89 0.91 0.87 0.84 0.72 0.6 0.64 0.57 0.64 0.58 0.96 0.97 0.98 0.93 0.94 0.87 0.94 0.92 0.91 0.86 0.87 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.09 0.08 0.17 0.11 0.24 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.07 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0.12 0.15 0.09 0.22 0.32 0.25 0.33 0.17 0.33 0 0.05 0 0.08 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 0 0 0 3 0 0 0 0 0 1 0 1 0 0 0 2
Bud 0 0 0 1 0 0 1 2 1 3 3 2 0 0 0 0 0 0 0 1 0 2 12 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 2 11 3 4 6 2 1 6 1 0 0 12 1 6 0 0 0 1 3 0
Cytoplasm 180 187 49 145 237 231 224 197 140 163 88 121 107 191 178 379 131 191 174 182 90 99 183 175
Endoplasmic Reticulum 0 1 1 1 0 6 1 1 5 5 1 3 0 0 0 8 4 3 0 0 1 0 1 2
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Golgi 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 1 1 6 0 0 0 0 0 0
Mitochondria 1 1 0 1 2 13 29 26 37 31 33 19 0 0 0 3 1 4 1 2 2 0 0 0
Nucleus 0 1 4 2 3 11 6 17 8 16 3 7 2 4 2 7 4 4 2 2 0 2 1 3
Nuclear Periphery 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 7 9 1 20 41 25 68 105 55 93 23 69 3 9 4 32 5 20 1 0 1 5 1 2
Unique Cell Count 188 197 55 160 273 275 311 330 219 284 137 210 111 197 181 409 139 220 186 199 99 116 210 204
Labelled Cell Count 190 199 56 171 288 297 332 354 252 313 152 232 113 205 184 442 147 236 186 199 99 116 210 204


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 9.2 7.9 7.8 9.5 9.3 9.6 9.8 9.0 10.2 10.5 11.4 9.4 13.2 14.9 21.8 21.2 21.2 13.0 12.2 15.9
Std Deviation (1e-4) 1.8 1.6 1.5 2.3 2.1 2.1 2.7 2.7 2.5 3.1 3.7 4.1 1.9 6.2 4.5 5.4 4.7 5.5 11.1 8.7 11.5
Intensity Change (Log2) -0.03 0.26 0.22 0.28 0.3 0.18 0.36 0.41 0.52 0.25 0.74 0.91 1.46 1.42 1.42 0.71 0.62 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.5 -1.0 -2.7 -4.2 -3.6 -4.4 -3.4 -4.3 1.9 2.4 3.1 0.9 1.3 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.3 2.6 4.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 2.7 0 3.5 4.6 3.8 4.7 2.8 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0283 11.5793 9.0099 8.3341 6.7892 8.7179 8.8666 8.926 8.9143 7.4518 6.3968 8.1206 6.2186 8.0968 8.0876 6.4209 5.8615 7.7373
Actin 0.0149 0.0006 0.0039 0.0225 0.0037 0.0008 0.0089 0.0004 0.0002 0.0012 0.0024 0.005 0.034 0.004 0.0235 0.0221 0.0731 0.0048
Bud 0.0004 0.0031 0.0004 0.0004 0.0009 0.0004 0.0003 0.0001 0.0001 0.0012 0.0002 0.0003 0.0018 0.0003 0.0005 0.0006 0.0002 0.0004
Bud Neck 0.0011 0.0004 0.001 0.0011 0.0012 0.0015 0.0005 0.0003 0.0004 0.001 0.001 0.0014 0.0014 0.0002 0.0004 0.0022 0.0006 0.0017
Bud Periphery 0.0005 0.0005 0.0004 0.0001 0.0013 0.0001 0.0003 0 0 0.0005 0.0001 0.0001 0.0029 0.0001 0.0006 0.0009 0.0003 0.0002
Bud Site 0.0023 0.001 0.0011 0.0003 0.0008 0.0001 0.0029 0.0002 0.0001 0.0007 0.0004 0.0002 0.0031 0.0003 0.0023 0.0018 0.0009 0.0009
Cell Periphery 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0.0005 0 0.0002 0.0002 0.0001 0
Cytoplasm 0.7765 0.9374 0.8572 0.8474 0.6697 0.8832 0.8756 0.9573 0.9288 0.8366 0.8033 0.8807 0.6863 0.9434 0.8845 0.7627 0.8067 0.8837
Cytoplasmic Foci 0.0232 0.0031 0.0137 0.0077 0.0598 0.0086 0.0037 0.002 0.0025 0.0165 0.016 0.0069 0.0359 0.0055 0.0105 0.0209 0.005 0.0075
Eisosomes 0.0006 0 0 0.0001 0 0 0.0001 0 0 0 0.0001 0 0.0002 0 0.0006 0.0002 0.0003 0
Endoplasmic Reticulum 0.0069 0.0012 0.0027 0.0024 0.0062 0.0022 0.0053 0.0011 0.0023 0.003 0.0381 0.0022 0.0078 0.0013 0.0024 0.0045 0.001 0.003
Endosome 0.0261 0.0014 0.0152 0.0033 0.0845 0.0068 0.0045 0.0006 0.0024 0.0026 0.0228 0.0062 0.053 0.003 0.0093 0.0271 0.0109 0.0182
Golgi 0.0102 0.0001 0.0081 0.0015 0.0386 0.0011 0.0016 0 0.0001 0.0003 0.0037 0.0015 0.0162 0.0023 0.0069 0.0127 0.0161 0.0047
Lipid Particles 0.0148 0 0.0019 0.0009 0.0057 0.0031 0.002 0 0 0.0017 0.0013 0.0003 0.0248 0.0011 0.0069 0.029 0.0357 0.0019
Mitochondria 0.0072 0.0002 0.0045 0.0009 0.0361 0.0041 0.0015 0.0001 0.0002 0.0004 0.0022 0.0019 0.0074 0.0021 0.004 0.0211 0.0135 0.0124
None 0.016 0.0081 0.0134 0.0197 0.0065 0.0071 0.0042 0.0019 0.0019 0.0054 0.0094 0.0021 0.007 0.0013 0.0026 0.0023 0.0012 0.0016
Nuclear Periphery 0.0077 0.0028 0.0051 0.0051 0.0112 0.0055 0.0073 0.0025 0.004 0.0078 0.0118 0.0046 0.0107 0.0029 0.0055 0.0232 0.0022 0.0081
Nucleolus 0.0005 0.0001 0.0001 0.0001 0.0011 0.0001 0.0001 0 0 0.0003 0.0002 0.0001 0.0005 0.0001 0.0002 0.0009 0.0001 0.0001
Nucleus 0.0728 0.0374 0.0627 0.0814 0.0418 0.0687 0.0731 0.0321 0.0551 0.0933 0.0738 0.0803 0.058 0.0284 0.0281 0.0398 0.0195 0.0443
Peroxisomes 0.011 0.0001 0.0033 0.001 0.0174 0.0019 0.0035 0.0001 0.0001 0.0017 0.0004 0.0015 0.0263 0.0023 0.0071 0.0151 0.0102 0.0006
Punctate Nuclear 0.0038 0.0013 0.0029 0.0027 0.0046 0.0029 0.002 0.0008 0.0009 0.024 0.0098 0.0032 0.0146 0.0007 0.0026 0.0084 0.0008 0.0029
Vacuole 0.0022 0.0009 0.0018 0.0011 0.0055 0.0012 0.0021 0.0004 0.0007 0.0015 0.0022 0.001 0.006 0.0005 0.0009 0.0025 0.0007 0.0017
Vacuole Periphery 0.0008 0.0001 0.0004 0.0002 0.0032 0.0003 0.0005 0.0001 0.0002 0.0003 0.0006 0.0003 0.0017 0.0002 0.0004 0.0017 0.0008 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.5318 17.7979 23.6332 39.9161 22.417 29.324 33.8598 36.437 47.7164 37.1585
Translational Efficiency 1.6113 1.6653 1.5327 1.3873 1.8531 2.3076 1.3059 1.4085 1.5635 1.4377

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dcs1

Dcs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dcs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available