Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 2 | 2 | 1 | 3 | 4 | 4 | 18 | 18 |
Bud | 0 | 2 | 1 | 0 | 3 | 8 | 18 | 14 | 20 | 14 | 12 | 13 | 2 | 0 | 0 | 1 | 7 | 2 | 0 | 0 | 0 | 2 | 4 | 10 |
Bud Neck | 0 | 0 | 6 | 9 | 14 | 8 | 16 | 18 | 9 | 15 | 4 | 9 | 8 | 3 | 2 | 1 | 3 | 0 | 0 | 3 | 1 | 0 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 5 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 1 | 2 | 2 | 3 | 0 | 0 | 0 | 1 | 4 | 5 |
Cytoplasm | 20 | 50 | 49 | 134 | 200 | 172 | 224 | 148 | 129 | 138 | 38 | 59 | 264 | 301 | 378 | 11 | 44 | 44 | 13 | 24 | 27 | 64 | 108 | 120 |
Endoplasmic Reticulum | 4 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 4 | 3 | 0 | 9 | 14 | 8 | 8 | 11 | 5 | 19 | 14 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 1 |
Mitochondria | 33 | 15 | 2 | 8 | 8 | 65 | 187 | 100 | 174 | 144 | 93 | 148 | 0 | 1 | 1 | 3 | 6 | 5 | 35 | 53 | 59 | 54 | 110 | 85 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 4 | 4 | 4 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 1 |
SpindlePole | 1 | 1 | 2 | 15 | 11 | 22 | 38 | 41 | 31 | 22 | 7 | 34 | 6 | 3 | 6 | 3 | 12 | 8 | 1 | 3 | 3 | 6 | 20 | 18 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 4 | 1 | 1 | 0 | 6 | 1 | 0 | 1 | 0 | 5 | 7 | 2 | 8 | 1 | 4 | 8 | 8 |
Unique Cell Count | 50 | 70 | 59 | 156 | 223 | 250 | 401 | 280 | 305 | 283 | 125 | 213 | 278 | 311 | 384 | 20 | 81 | 82 | 67 | 111 | 117 | 152 | 316 | 310 |
Labelled Cell Count | 59 | 79 | 67 | 168 | 240 | 286 | 497 | 338 | 378 | 345 | 162 | 279 | 287 | 319 | 397 | 25 | 93 | 92 | 67 | 111 | 117 | 152 | 316 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.9 | 3.9 | 4.3 | 4.4 | 4.3 | 3.9 | 3.3 | 3.8 | 3.1 | 3.3 | 3.2 | 3.2 | 4.7 | 5.0 | 5.1 | 5.7 | 5.6 | 5.7 | 4.6 | 5.0 | 5.5 |
Std Deviation (1e-4) | 0.5 | 0.5 | 0.7 | 1.7 | 1.4 | 2.0 | 1.4 | 1.5 | 1.4 | 1.1 | 1.5 | 0.6 | 1.0 | 1.1 | 1.1 | 1.3 | 1.6 | 1.9 | 1.1 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.02 | -0.15 | -0.37 | -0.18 | -0.45 | -0.39 | -0.41 | -0.43 | 0.12 | 0.21 | 0.24 | 0.41 | 0.38 | 0.41 | 0.09 | 0.22 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.346 | -1.0374 | -1.0506 | -1.0975 | -1.468 | -1.017 | -1.0056 | -0.555 | -0.4587 | -0.7905 | -0.9219 | -0.3872 | -1.4285 | -1.1286 | -1.2417 | -1.4321 | -1.754 | -1.2841 |
Actin | 0.0079 | 0.0057 | 0.0096 | 0.0119 | 0.0017 | 0.0049 | 0.004 | 0.0048 | 0.0023 | 0.0004 | 0.0024 | 0.0076 | 0.0301 | 0.003 | 0.0267 | 0.0053 | 0.0463 | 0.0106 |
Bud | 0.0005 | 0.0018 | 0.0028 | 0.0012 | 0.0046 | 0.0014 | 0.0009 | 0.0006 | 0.0023 | 0.0008 | 0.0045 | 0.0007 | 0.0028 | 0.0088 | 0.0121 | 0.0017 | 0.0017 | 0.0028 |
Bud Neck | 0.0009 | 0.0008 | 0.0046 | 0.0014 | 0.0029 | 0.0053 | 0.0009 | 0.0021 | 0.0048 | 0.0008 | 0.0004 | 0.0062 | 0.0027 | 0.0006 | 0.0054 | 0.0344 | 0.0012 | 0.0096 |
Bud Periphery | 0.0011 | 0.0013 | 0.0036 | 0.0027 | 0.0025 | 0.0032 | 0.002 | 0.0013 | 0.0026 | 0.0013 | 0.0073 | 0.0012 | 0.0036 | 0.0035 | 0.0104 | 0.0039 | 0.0034 | 0.0065 |
Bud Site | 0.003 | 0.0074 | 0.005 | 0.0038 | 0.0073 | 0.0004 | 0.003 | 0.0241 | 0.0092 | 0.0012 | 0.0061 | 0.0033 | 0.0087 | 0.0321 | 0.0132 | 0.0021 | 0.001 | 0.0012 |
Cell Periphery | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0006 | 0.0006 | 0.0002 | 0.0021 | 0.0002 | 0.0008 | 0.0011 | 0.0014 | 0.0008 | 0.0004 | 0.0004 |
Cytoplasm | 0.0463 | 0.0501 | 0.0422 | 0.0382 | 0.0487 | 0.0552 | 0.0489 | 0.0786 | 0.0655 | 0.0663 | 0.0593 | 0.0909 | 0.0788 | 0.0903 | 0.0965 | 0.0965 | 0.0669 | 0.1445 |
Cytoplasmic Foci | 0.1348 | 0.0984 | 0.1335 | 0.1198 | 0.1161 | 0.1475 | 0.1198 | 0.152 | 0.1157 | 0.1574 | 0.0838 | 0.1012 | 0.1987 | 0.1226 | 0.1521 | 0.1159 | 0.2455 | 0.1558 |
Eisosomes | 0.0005 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0018 | 0.0005 | 0.0015 | 0.0009 | 0.0007 | 0.0004 |
Endoplasmic Reticulum | 0.0015 | 0.0013 | 0.001 | 0.0009 | 0.0011 | 0.001 | 0.0016 | 0.0007 | 0.0011 | 0.0008 | 0.001 | 0.0009 | 0.0045 | 0.0014 | 0.0027 | 0.0024 | 0.0032 | 0.0024 |
Endosome | 0.0193 | 0.0226 | 0.0178 | 0.0198 | 0.014 | 0.0192 | 0.0222 | 0.0276 | 0.0204 | 0.0098 | 0.004 | 0.011 | 0.0559 | 0.0477 | 0.0381 | 0.0149 | 0.094 | 0.0352 |
Golgi | 0.006 | 0.0046 | 0.0054 | 0.0056 | 0.0051 | 0.005 | 0.0034 | 0.0064 | 0.0045 | 0.0025 | 0.002 | 0.0023 | 0.0133 | 0.0102 | 0.0116 | 0.0043 | 0.0295 | 0.0064 |
Lipid Particles | 0.0144 | 0.0076 | 0.0174 | 0.0083 | 0.0082 | 0.0091 | 0.0126 | 0.0069 | 0.006 | 0.0057 | 0.0051 | 0.0069 | 0.0254 | 0.007 | 0.0091 | 0.0036 | 0.0126 | 0.0084 |
Mitochondria | 0.0061 | 0.005 | 0.0098 | 0.0128 | 0.0061 | 0.0079 | 0.0145 | 0.02 | 0.0307 | 0.0051 | 0.0207 | 0.0118 | 0.0143 | 0.0098 | 0.0266 | 0.0235 | 0.0209 | 0.0112 |
None | 0.7177 | 0.7525 | 0.6856 | 0.6615 | 0.7407 | 0.6909 | 0.7301 | 0.6443 | 0.6811 | 0.7035 | 0.7349 | 0.7151 | 0.4995 | 0.6239 | 0.5176 | 0.6498 | 0.382 | 0.5638 |
Nuclear Periphery | 0.0014 | 0.0007 | 0.0016 | 0.0013 | 0.0012 | 0.0007 | 0.0007 | 0.0004 | 0.0009 | 0.0004 | 0.0006 | 0.0009 | 0.0051 | 0.0008 | 0.0014 | 0.0011 | 0.0019 | 0.0012 |
Nucleolus | 0.0011 | 0.0011 | 0.0019 | 0.0015 | 0.0007 | 0.0007 | 0.0017 | 0.0011 | 0.0019 | 0.0013 | 0.0008 | 0.0006 | 0.0011 | 0.0024 | 0.0058 | 0.0012 | 0.0008 | 0.0006 |
Nucleus | 0.0015 | 0.0018 | 0.0055 | 0.0022 | 0.0016 | 0.0017 | 0.0015 | 0.0018 | 0.0034 | 0.0012 | 0.0018 | 0.002 | 0.0021 | 0.0068 | 0.0105 | 0.002 | 0.0015 | 0.0026 |
Peroxisomes | 0.0288 | 0.0247 | 0.0374 | 0.0917 | 0.0243 | 0.0341 | 0.0238 | 0.0225 | 0.0358 | 0.0377 | 0.0459 | 0.0276 | 0.0393 | 0.015 | 0.0457 | 0.0258 | 0.069 | 0.0273 |
Punctate Nuclear | 0.0056 | 0.0099 | 0.0125 | 0.012 | 0.0105 | 0.01 | 0.0026 | 0.0021 | 0.008 | 0.002 | 0.0159 | 0.0079 | 0.0068 | 0.0092 | 0.0061 | 0.0075 | 0.0047 | 0.0056 |
Vacuole | 0.0012 | 0.0017 | 0.0013 | 0.002 | 0.0017 | 0.0012 | 0.0037 | 0.0015 | 0.0023 | 0.0011 | 0.0009 | 0.0011 | 0.0036 | 0.0029 | 0.0043 | 0.0019 | 0.0097 | 0.0025 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0005 | 0.0004 | 0.0007 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0011 | 0.0005 | 0.0012 | 0.0005 | 0.0031 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.4219 | 8.3503 | 6.9708 | 12.116 | 17.4096 | 11.6698 | 10.8175 | 8.1915 | 13.0136 | 14.4639 |
Translational Efficiency | 1.0515 | 0.7328 | 0.6455 | 0.5137 | 0.3421 | 0.6871 | 0.5466 | 0.9753 | 0.586 | 0.3738 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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