Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; novel N-terminal protein methyltransferase that trimethylates the N-terminal glycine residue (G2) and also dimethylates lysine (K3) on elongation factor eEF1A (Tef1p/Tef2p); has a role in rDNA silencing and in lifespan determination
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0.07 0 0 0.09 0.09 0.08 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.96 0.95 0.91 0.82 0.79 0.79 0.78 0.79 0.71 0.98 0.99 0.98 0.98 0.96 0.97 0.94 0.93 0.94 0.95 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.05 0.17 0.15 0.15 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.12 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 4 4 1 8 10 9 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 2 1 5 3 3 2 0 0 0 0 0 0 3 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 10 15 9 8 22 9 11 32 32 18 1 6 8 6 15 6 0 0 0 0 0 0
Cytoplasm 196 145 254 227 277 271 271 206 232 282 282 156 64 182 188 140 171 150 184 142 246 108 157 159
Endoplasmic Reticulum 2 1 4 2 11 14 27 14 12 14 13 7 1 2 3 6 3 6 3 2 4 0 1 1
Endosome 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 3 1 1 1 8 21 14 51 53 53 56 0 0 1 3 1 3 0 1 1 0 0 1
Nucleus 0 0 5 0 0 0 2 3 2 4 4 2 0 0 1 1 4 2 0 0 1 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 0 5 4 10 15 31 9 14 18 13 1 0 0 0 1 1 0 2 1 1 0 1
Unique Cell Count 198 148 265 237 292 299 332 262 295 360 359 221 65 183 191 143 179 154 196 153 264 114 166 172
Labelled Cell Count 207 152 275 251 306 315 366 279 331 415 415 263 67 190 201 156 195 168 196 153 264 114 166 172


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 13.0 14.2 13.6 12.4 10.6 10.5 9.9 8.9 9.1 9.1 8.7 14.2 13.9 13.2 13.5 17.1 18.8 14.1 13.9 14.9
Std Deviation (1e-4) 4.1 3.4 2.8 2.6 2.8 2.3 2.3 2.2 2.0 2.0 2.0 1.7 3.2 2.8 3.0 2.1 3.0 3.9 3.6 3.6 3.7
Intensity Change (Log2) – – – -0.07 -0.2 -0.42 -0.44 -0.52 -0.68 -0.65 -0.65 -0.7 -0.0 -0.04 -0.11 -0.08 0.26 0.4 -0.02 -0.03 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.3 -0.4 -0.7 1.5 -0.2 0 2.5 2.5 2.1 0 -0.3 0.2 0.2 2.1 0.1
Cytoplasm 0 -0.6 -2.4 -5.3 -5.9 -6.0 -6.2 -6.1 -7.6 1.0 2.3 1.6 1.1 -0.2 0.8
Endoplasmic Reticulum 0 1.6 2.1 3.6 2.4 1.8 1.8 1.6 1.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 3.4 6.9 6.3 6.3 8.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.5 5.8 0 3.2 3.7 4.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3756 12.1704 9.1335 10.109 8.6361 9.977 10.1387 13.4124 12.2854 11.5247 11.1693 12.6727 8.2534 12.1155 10.1819 9.6097 10.5645 10.9664
Actin 0.016 0.0005 0.0063 0.0003 0.0013 0.0006 0.009 0.0001 0.0025 0.0244 0.0035 0.0037 0.0271 0.0006 0.0148 0.0227 0.0014 0.0025
Bud 0.0005 0.0003 0.0008 0.0004 0.0023 0.0002 0.0008 0.0002 0.0003 0.0003 0.0001 0.0002 0.0008 0.0002 0.0005 0.0005 0.0002 0.0003
Bud Neck 0.0025 0.0003 0.0017 0.0004 0.0014 0.0007 0.0124 0.0001 0.0003 0.0005 0.0005 0.0007 0.0015 0.0001 0.0008 0.0005 0.0004 0.0007
Bud Periphery 0.0004 0.0001 0.0006 0.0001 0.0037 0.0001 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001 0.0015 0 0.0004 0.0003 0.0001 0.0002
Bud Site 0.0023 0.0015 0.0028 0.0002 0.0008 0.0001 0.0035 0.0004 0.0018 0.0002 0.0003 0.0001 0.0043 0.0004 0.0063 0.0006 0.0003 0.0005
Cell Periphery 0.0004 0.0004 0.0004 0.0001 0.0002 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0.0001 0.0011 0.0001 0.0003 0.0001 0 0
Cytoplasm 0.809 0.9768 0.9191 0.9439 0.8471 0.929 0.8554 0.9688 0.9618 0.9008 0.9345 0.9654 0.815 0.9784 0.9414 0.8999 0.9494 0.9415
Cytoplasmic Foci 0.0206 0.0029 0.01 0.0088 0.0136 0.0064 0.0126 0.0029 0.0093 0.0108 0.0074 0.0057 0.0141 0.0021 0.0058 0.0217 0.0078 0.008
Eisosomes 0.0001 0 0.0001 0 0 0 0.0004 0 0 0.0001 0.0001 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0369 0.0077 0.0161 0.0018 0.0421 0.0161 0.0261 0.003 0.0049 0.0124 0.0025 0.0103 0.0339 0.0139 0.0035 0.0025 0.0023 0.0123
Endosome 0.0468 0.0038 0.0154 0.0227 0.0467 0.017 0.0166 0.0032 0.0057 0.0278 0.0125 0.0077 0.0349 0.0015 0.0089 0.0165 0.0056 0.0117
Golgi 0.0098 0.0005 0.0019 0.0009 0.0055 0.0036 0.0052 0.0002 0.0006 0.0026 0.0008 0.0013 0.0117 0.0002 0.0038 0.013 0.0025 0.0064
Lipid Particles 0.0072 0.0001 0.0018 0.0001 0.001 0 0.0015 0 0.0006 0.0008 0.0017 0.0003 0.009 0 0.0006 0.0026 0.0008 0.0071
Mitochondria 0.0046 0.0003 0.0012 0.0001 0.009 0.0203 0.005 0.0002 0.0016 0.0004 0.0002 0.0009 0.0041 0.0001 0.0056 0.0012 0.0205 0.0023
None 0.0082 0.002 0.0033 0.0008 0.0015 0.0005 0.0265 0.0183 0.0046 0.0012 0.0136 0.0008 0.0044 0.0006 0.0017 0.0015 0.0006 0.0029
Nuclear Periphery 0.0097 0.0005 0.0079 0.0009 0.004 0.0008 0.0036 0.0003 0.0006 0.002 0.003 0.0005 0.011 0.0005 0.0006 0.0008 0.0007 0.0005
Nucleolus 0.0004 0 0.0002 0 0.0005 0 0.0009 0 0 0 0.0005 0 0.0003 0 0.0002 0 0 0
Nucleus 0.0046 0.0007 0.0017 0.0012 0.0044 0.0008 0.0057 0.0005 0.0007 0.0014 0.0015 0.0006 0.0083 0.0005 0.0008 0.001 0.0011 0.0007
Peroxisomes 0.005 0 0.0007 0 0.0004 0 0.0058 0 0.0009 0.0002 0.001 0.0004 0.0057 0 0.0004 0.0088 0.0047 0.0005
Punctate Nuclear 0.0036 0.0001 0.0018 0.0002 0.0012 0 0.0029 0 0.0006 0.0002 0.0128 0.0001 0.0026 0 0.0002 0.0005 0.0001 0.0001
Vacuole 0.0091 0.0016 0.0048 0.0148 0.0105 0.0026 0.0039 0.0015 0.0024 0.0119 0.0027 0.0009 0.0063 0.0006 0.0028 0.0047 0.0011 0.0013
Vacuole Periphery 0.0023 0.0002 0.0013 0.0024 0.0027 0.001 0.0006 0.0002 0.0003 0.0017 0.0005 0.0003 0.002 0.0001 0.0005 0.0007 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.7975 25.0986 30.73 44.3946 39.1325 33.6731 40.2863 33.8276 38.4447 41.9444
Translational Efficiency 2.2292 2.8055 2.2387 1.3916 1.6951 2.468 1.574 1.6632 1.7583 1.9448

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; novel N-terminal protein methyltransferase that trimethylates the N-terminal glycine residue (G2) and also dimethylates lysine (K3) on elongation factor eEF1A (Tef1p/Tef2p); has a role in rDNA silencing and in lifespan determination
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Nnt1

Nnt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nnt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available