Standard name | |
---|---|
Human Ortholog | |
Description | Beta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; human homolog EIF2B2 can complement yeast mutant, allows growth down-regulation of yeast gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 2 | 7 | 13 | 1 | 2 | 8 | 12 | 9 | 18 | 12 | 5 | 16 | 5 | 0 | 5 | 7 | 11 | 5 | 3 | 2 | 5 | 11 | 13 |
Bud | 0 | 1 | 6 | 13 | 0 | 4 | 2 | 5 | 6 | 4 | 9 | 11 | 3 | 0 | 2 | 0 | 3 | 4 | 17 | 9 | 27 | 20 | 46 | 52 |
Bud Neck | 3 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 25 | 18 | 3 | 13 | 2 | 4 | 4 | 7 | 7 | 6 | 9 |
Bud Site | 4 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 3 | 3 | 8 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 7 | 11 | 11 | 11 | 23 | 39 | 16 | 38 | 28 | 32 | 6 | 1 | 0 | 1 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 51 | 45 | 101 | 140 | 255 | 248 | 349 | 302 | 148 | 193 | 159 | 208 | 210 | 94 | 123 | 78 | 149 | 110 | 55 | 60 | 87 | 155 | 264 | 291 |
Endoplasmic Reticulum | 1 | 1 | 2 | 1 | 5 | 8 | 27 | 50 | 14 | 31 | 21 | 7 | 7 | 0 | 0 | 8 | 7 | 8 | 2 | 0 | 10 | 5 | 12 | 10 |
Endosome | 35 | 1 | 16 | 14 | 0 | 1 | 3 | 3 | 0 | 0 | 2 | 7 | 18 | 56 | 34 | 23 | 23 | 23 | 15 | 2 | 11 | 27 | 46 | 56 |
Golgi | 1 | 4 | 3 | 5 | 6 | 1 | 1 | 8 | 1 | 0 | 3 | 1 | 10 | 2 | 2 | 7 | 13 | 10 | 3 | 0 | 2 | 2 | 10 | 9 |
Mitochondria | 12 | 2 | 7 | 31 | 30 | 95 | 179 | 129 | 149 | 126 | 169 | 190 | 3 | 1 | 4 | 26 | 6 | 20 | 0 | 0 | 2 | 1 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 4 | 10 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 19 | 13 | 19 | 13 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 23 | 151 | 171 | 60 | 62 | 75 | 2 | 4 | 5 | 5 | 11 | 6 |
Peroxisomes | 13 | 0 | 5 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 20 | 55 | 63 | 5 | 8 | 15 | 1 | 0 | 0 | 2 | 5 | 5 |
SpindlePole | 22 | 2 | 39 | 41 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 67 | 270 | 238 | 52 | 67 | 59 | 45 | 12 | 36 | 41 | 127 | 119 |
Vac/Vac Membrane | 6 | 6 | 7 | 19 | 44 | 10 | 17 | 53 | 14 | 17 | 19 | 36 | 8 | 14 | 9 | 33 | 51 | 60 | 0 | 1 | 3 | 7 | 12 | 15 |
Unique Cell Count | 135 | 70 | 183 | 248 | 327 | 327 | 512 | 513 | 282 | 336 | 336 | 404 | 327 | 505 | 518 | 228 | 333 | 307 | 157 | 103 | 205 | 289 | 567 | 607 |
Labelled Cell Count | 173 | 78 | 223 | 324 | 356 | 381 | 612 | 607 | 358 | 431 | 423 | 500 | 393 | 682 | 676 | 307 | 418 | 410 | 157 | 103 | 205 | 289 | 567 | 607 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.5 | 16.3 | 13.4 | 13.6 | 14.9 | 9.9 | 9.2 | 9.7 | 8.5 | 9.0 | 7.9 | 7.8 | 16.7 | 16.5 | 16.0 | 18.1 | 19.0 | 17.7 | 16.8 | 17.7 | 17.7 |
Std Deviation (1e-4) | 2.7 | 3.9 | 3.0 | 4.0 | 7.8 | 2.0 | 1.9 | 2.4 | 2.2 | 2.9 | 1.8 | 1.8 | 3.5 | 4.5 | 5.8 | 7.7 | 7.6 | 7.3 | 3.3 | 3.9 | 4.3 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.16 | -0.43 | -0.54 | -0.46 | -0.65 | -0.57 | -0.75 | -0.77 | 0.32 | 0.31 | 0.26 | 0.44 | 0.51 | 0.4 | 0.33 | 0.4 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.7 | 0 | 0 | 0 | 0 | -0.4 | 0.8 | -0.1 | 0 | 0.6 | 0 | 0 | -1.0 | 0 | -0.1 |
Bud | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 1.7 | 0 | 1.8 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -0.3 | -0.3 | 0.4 | 1.8 | 0.9 | 2.9 | 2.0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 5.4 | 4.8 | 3.2 | 0.9 | -0.6 | 0.5 | -1.7 | -0.8 | 2.0 | -9.4 | -7.8 | -4.3 | -2.3 | -4.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.4 | 3.8 | 2.2 | 3.6 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.2 | -5.4 | -5.1 | -5.8 | 0 | -5.1 | -5.5 | -4.8 | -4.1 | -1.4 | 0.9 | -1.0 | 0.5 | -0.8 | -0.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 |
Mitochondria | 3.1 | 2.2 | 6.8 | 8.2 | 6.2 | 10.9 | 8.4 | 10.7 | 10.3 | 0 | 0 | 0 | 2.8 | 0 | 1.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.0 | -5.6 | -5.6 | -7.1 | -7.1 | -5.5 | -5.7 | -6.0 | -6.6 | -1.3 | 5.2 | 5.9 | 4.1 | 2.5 | 3.8 |
Peroxisomes | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 3.4 | 3.7 | 0 | 0 | 1.2 |
SpindlePole | -1.3 | -8.2 | -8.7 | -10.8 | -10.8 | -7.9 | -8.8 | -8.8 | -9.4 | -0.2 | 7.5 | 5.9 | 0.4 | -0.3 | -0.6 |
Vacuole | 1.7 | 3.5 | -0.5 | -0.3 | 2.7 | 0.6 | 0.6 | 0.9 | 2.2 | -0.9 | -0.7 | 0 | 3.6 | 4.0 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.6942 | 17.3015 | 14.8976 | 12.9032 | 15.0762 | 14.241 | 7.7419 | 18.3664 | 13.6109 | 11.0302 | 13.4612 | 13.8542 | 6.4025 | 15.3178 | 12.5494 | 10.4912 | 9.4505 | 12.2565 |
Actin | 0.0088 | 0.0064 | 0.0088 | 0.0005 | 0 | 0.0043 | 0.0142 | 0.0018 | 0.0108 | 0.0566 | 0.0006 | 0.0069 | 0.0172 | 0.0035 | 0.0072 | 0.0009 | 0.0011 | 0.0013 |
Bud | 0.0089 | 0.0207 | 0.0133 | 0.001 | 0.0079 | 0.01 | 0.0041 | 0.0217 | 0.0222 | 0.0221 | 0.0361 | 0.015 | 0.0099 | 0.0179 | 0.0209 | 0.0004 | 0.0026 | 0.0101 |
Bud Neck | 0.0087 | 0.0027 | 0.0044 | 0.0021 | 0.0038 | 0.0138 | 0.002 | 0.005 | 0.0071 | 0.0458 | 0.0037 | 0.0208 | 0.0062 | 0.0053 | 0.0045 | 0.0008 | 0.0027 | 0.0158 |
Bud Periphery | 0.0053 | 0.0067 | 0.004 | 0.0005 | 0.003 | 0.0045 | 0.0021 | 0.0054 | 0.0091 | 0.0126 | 0.014 | 0.006 | 0.0034 | 0.0058 | 0.008 | 0.0002 | 0.0011 | 0.009 |
Bud Site | 0.0255 | 0.0853 | 0.0235 | 0.0066 | 0.0172 | 0.0066 | 0.0156 | 0.101 | 0.0295 | 0.0337 | 0.0101 | 0.0101 | 0.019 | 0.0489 | 0.0498 | 0.0019 | 0.0109 | 0.0053 |
Cell Periphery | 0.0012 | 0.002 | 0.0008 | 0.0007 | 0.0012 | 0.0005 | 0.0015 | 0.0033 | 0.0021 | 0.0024 | 0.0034 | 0.0028 | 0.0028 | 0.0032 | 0.0041 | 0.0005 | 0.0003 | 0.0019 |
Cytoplasm | 0.0521 | 0.0911 | 0.1079 | 0.0046 | 0.0294 | 0.0429 | 0.0975 | 0.1137 | 0.1829 | 0.0966 | 0.0146 | 0.0634 | 0.0951 | 0.0823 | 0.0572 | 0.0461 | 0.0513 | 0.0731 |
Cytoplasmic Foci | 0.6586 | 0.5669 | 0.6193 | 0.7132 | 0.6533 | 0.7174 | 0.5141 | 0.5125 | 0.4356 | 0.3403 | 0.565 | 0.498 | 0.5308 | 0.6198 | 0.5734 | 0.7691 | 0.6818 | 0.6647 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0008 | 0.0057 | 0.0012 | 0.0005 | 0.0022 | 0.0072 | 0.0004 | 0.0019 | 0.0042 | 0.0001 | 0.0173 | 0.0134 | 0.0002 | 0.0051 | 0.0002 | 0.003 | 0.0044 |
Endosome | 0.0755 | 0.0629 | 0.0709 | 0.0443 | 0.0536 | 0.0776 | 0.0821 | 0.0345 | 0.0878 | 0.0886 | 0.1304 | 0.0986 | 0.0979 | 0.0323 | 0.049 | 0.0381 | 0.0703 | 0.0633 |
Golgi | 0.0156 | 0.0085 | 0.0111 | 0.0078 | 0.0045 | 0.0192 | 0.0176 | 0.0078 | 0.0136 | 0.009 | 0.0413 | 0.0197 | 0.0136 | 0.0042 | 0.0137 | 0.0033 | 0.0086 | 0.0069 |
Lipid Particles | 0.0448 | 0.0226 | 0.0372 | 0.0407 | 0.0324 | 0.0268 | 0.0327 | 0.0307 | 0.019 | 0.01 | 0.0186 | 0.0532 | 0.0534 | 0.0403 | 0.0404 | 0.0415 | 0.0641 | 0.0463 |
Mitochondria | 0.0057 | 0.0034 | 0.0083 | 0.0025 | 0.0044 | 0.004 | 0.0302 | 0.0126 | 0.045 | 0.0574 | 0.0098 | 0.0192 | 0.0119 | 0.0122 | 0.0133 | 0.0008 | 0.0059 | 0.008 |
None | 0.0035 | 0.0014 | 0.0024 | 0.0004 | 0.0003 | 0.0009 | 0.0076 | 0.0022 | 0.0009 | 0.0012 | 0.0003 | 0.001 | 0.0122 | 0.0011 | 0.0029 | 0.0002 | 0.0019 | 0.0006 |
Nuclear Periphery | 0.001 | 0.0003 | 0.0029 | 0.0016 | 0.0002 | 0.0026 | 0.0206 | 0.0008 | 0.003 | 0.0036 | 0.0002 | 0.0029 | 0.0056 | 0.0003 | 0.0007 | 0.0001 | 0.0055 | 0.004 |
Nucleolus | 0.0188 | 0.0245 | 0.0151 | 0.015 | 0.1139 | 0.0139 | 0.0336 | 0.0641 | 0.0507 | 0.0879 | 0.0591 | 0.0625 | 0.026 | 0.0398 | 0.0533 | 0.0634 | 0.0291 | 0.0152 |
Nucleus | 0.0024 | 0.0027 | 0.0044 | 0.0029 | 0.0083 | 0.0051 | 0.0105 | 0.0062 | 0.0094 | 0.0148 | 0.0043 | 0.005 | 0.0088 | 0.0043 | 0.0103 | 0.0039 | 0.0087 | 0.0088 |
Peroxisomes | 0.0342 | 0.0281 | 0.0199 | 0.0317 | 0.0257 | 0.02 | 0.0357 | 0.0242 | 0.015 | 0.0197 | 0.0068 | 0.0248 | 0.0282 | 0.0364 | 0.0309 | 0.0158 | 0.0261 | 0.0198 |
Punctate Nuclear | 0.0097 | 0.0116 | 0.0079 | 0.0112 | 0.0174 | 0.011 | 0.0049 | 0.0051 | 0.0044 | 0.0062 | 0.0015 | 0.0078 | 0.015 | 0.0057 | 0.0036 | 0.0026 | 0.0131 | 0.0093 |
Vacuole | 0.0142 | 0.0483 | 0.0295 | 0.1036 | 0.0219 | 0.0142 | 0.056 | 0.0431 | 0.0407 | 0.0722 | 0.0716 | 0.0544 | 0.0248 | 0.0342 | 0.0463 | 0.0094 | 0.0099 | 0.0285 |
Vacuole Periphery | 0.0018 | 0.0029 | 0.0027 | 0.0077 | 0.0014 | 0.0022 | 0.01 | 0.0038 | 0.009 | 0.0149 | 0.0085 | 0.0105 | 0.0044 | 0.0023 | 0.0054 | 0.0008 | 0.0019 | 0.0038 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.1026 | 55.9463 | 54.2883 | 67.212 | 59.2967 | 45.228 | 49.17 | 49.0454 | 46.4503 | 71.2038 |
Translational Efficiency | 0.885 | 0.9009 | 0.9145 | 0.7065 | 0.8901 | 1.2168 | 1.079 | 0.8426 | 1.039 | 0.9064 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|