Standard name | |
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Human Ortholog | |
Description | Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 10 | 22 | 46 | 48 | 43 | 49 | 44 | – | 62 | 42 | 65 | 12 | 23 | 17 | 2 | – | – | 2 | 2 | 5 | 2 | 2 | 7 |
Bud | 0 | 0 | 1 | 4 | 3 | 9 | 16 | 11 | – | 23 | 33 | 24 | 0 | 0 | 0 | 0 | – | – | 2 | 2 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 24 | 15 | 18 | 37 | 31 | 33 | 37 | 32 | – | 45 | 60 | 52 | 32 | 55 | 88 | 14 | – | – | 2 | 4 | 3 | 1 | 4 | 7 |
Cytoplasm | 28 | 35 | 13 | 38 | 48 | 69 | 100 | 83 | – | 78 | 106 | 103 | 24 | 49 | 53 | 8 | – | – | 5 | 6 | 1 | 5 | 3 | 5 |
Endoplasmic Reticulum | 348 | 226 | 140 | 178 | 267 | 275 | 327 | 372 | – | 284 | 172 | 292 | 223 | 326 | 327 | 1 | – | – | 382 | 255 | 149 | 177 | 210 | 232 |
Endosome | 0 | 0 | 0 | 2 | 3 | 1 | 10 | 2 | – | 4 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Golgi | 9 | 2 | 1 | 11 | 11 | 1 | 1 | 1 | – | 1 | 0 | 0 | 3 | 11 | 2 | 0 | – | – | 3 | 1 | 0 | 1 | 3 | 7 |
Mitochondria | 1 | 3 | 12 | 51 | 56 | 69 | 156 | 113 | – | 120 | 137 | 176 | 0 | 0 | 0 | 2 | – | – | 1 | 0 | 0 | 1 | 2 | 7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 1 | – | 2 | 2 | 3 | 0 | 1 | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 2 | 3 | 3 | 4 | 11 | – | 16 | 9 | 8 | 0 | 1 | 1 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 4 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 26 | 1 | 2 | 22 | 11 | 27 | 80 | 41 | – | 107 | 79 | 49 | 5 | 4 | 12 | 0 | – | – | 1 | 3 | 1 | 7 | 4 | 9 |
Unique Cell Count | 386 | 265 | 159 | 270 | 360 | 412 | 598 | 584 | 569 | 493 | 572 | 263 | 382 | 410 | 24 | 405 | 280 | 164 | 202 | 238 | 287 | |||
Labelled Cell Count | 445 | 292 | 211 | 391 | 481 | 534 | 785 | 712 | 742 | 645 | 772 | 299 | 470 | 500 | 27 | 405 | 280 | 164 | 202 | 238 | 287 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.1 | 18.0 | 11.9 | 11.2 | 11.0 | 10.4 | 9.7 | 9.8 | – | 9.3 | 9.7 | 9.3 | 15.2 | 15.9 | 15.8 | 16.4 | – | – | 17.3 | 17.3 | 17.5 |
Std Deviation (1e-4) | 2.7 | 3.1 | 2.1 | 1.8 | 1.9 | 1.9 | 1.8 | 1.7 | – | 1.9 | 1.9 | 2.1 | 2.5 | 2.7 | 2.7 | 2.7 | – | – | 3.1 | 3.8 | 3.9 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.12 | -0.19 | -0.29 | -0.28 | – | -0.35 | -0.3 | -0.35 | 0.36 | 0.42 | 0.42 | 0.47 | – | – | 0.54 | 0.54 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | -0.2 | -1.1 | -2.2 | -2.5 | – | -1.0 | -2.0 | -0.9 | -3.4 | -3.0 | -4.1 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 2.1 | 3.0 | 2.2 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0.7 | -1.0 | -1.2 | -2.2 | -2.6 | – | -1.4 | 0.3 | -0.8 | 0.3 | 1.0 | 2.8 | 0 | – | – |
Cytoplasm | 1.8 | 1.7 | 2.6 | 2.7 | 2.0 | – | 1.9 | 3.8 | 3.0 | 0.3 | 1.5 | 1.6 | 0 | – | – |
Endoplasmic Reticulum | -5.1 | -3.5 | -5.1 | -7.7 | -5.9 | – | -8.6 | -11.7 | -8.4 | -0.9 | -0.8 | -2.3 | -9.1 | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 3.2 | 2.5 | 2.8 | 5.0 | 3.5 | – | 3.9 | 5.3 | 5.9 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | 3.0 | 0 | 2.6 | 4.4 | 2.8 | – | 5.5 | 4.9 | 3.2 | 0 | 0 | 0 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.395 | 11.5807 | 10.7165 | 9.9749 | 9.2959 | 10.8994 | 12.2441 | 13.9698 | 12.884 | 12.8571 | 10.7663 | 12.46 | 12.2449 | 12.2344 | 11.9591 | 10.4139 | 11.384 | 11.6436 |
Actin | 0.0065 | 0.0031 | 0.0127 | 0.011 | 0.0033 | 0.018 | 0.0306 | 0.0025 | 0.0103 | 0 | 0.0018 | 0.0028 | 0.0004 | 0.0003 | 0.0012 | 0.0001 | 0.0041 | 0.0016 |
Bud | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0006 | 0.0012 | 0.0146 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 |
Bud Periphery | 0.0006 | 0.0002 | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.008 | 0.001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0003 |
Bud Site | 0.0005 | 0.0021 | 0.0064 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0015 | 0.0044 | 0 | 0.0008 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0018 | 0.0068 | 0.0018 | 0.0041 | 0.0005 | 0.0004 | 0.0015 | 0.0083 | 0.0019 | 0.0004 | 0.001 | 0.0009 | 0.0013 | 0.0052 | 0.0019 | 0.0005 | 0.0006 | 0.002 |
Cytoplasm | 0.0282 | 0.059 | 0.0402 | 0.0179 | 0.0128 | 0.0113 | 0.0127 | 0.0032 | 0.0067 | 0.0019 | 0.0021 | 0.0145 | 0.0041 | 0.0158 | 0.0048 | 0.0078 | 0.0014 | 0.0046 |
Cytoplasmic Foci | 0.0095 | 0.0022 | 0.0101 | 0.005 | 0.0174 | 0.0011 | 0.0034 | 0.0002 | 0.0017 | 0 | 0.0019 | 0.0042 | 0.0007 | 0.0006 | 0.0002 | 0.0005 | 0.002 | 0.0001 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.8499 | 0.8857 | 0.8496 | 0.828 | 0.8537 | 0.9104 | 0.8905 | 0.9345 | 0.9445 | 0.9917 | 0.8705 | 0.9409 | 0.9585 | 0.9488 | 0.9745 | 0.9834 | 0.8943 | 0.9764 |
Endosome | 0.0346 | 0.0044 | 0.0202 | 0.043 | 0.0335 | 0.0103 | 0.0123 | 0.0015 | 0.0063 | 0.0007 | 0.0185 | 0.0097 | 0.0049 | 0.002 | 0.001 | 0.0024 | 0.0114 | 0.0018 |
Golgi | 0.0147 | 0.0038 | 0.009 | 0.0252 | 0.005 | 0.0048 | 0.0076 | 0.0006 | 0.0055 | 0.0001 | 0.0038 | 0.0069 | 0.0007 | 0.0017 | 0.0076 | 0.0006 | 0.0118 | 0.0011 |
Lipid Particles | 0.015 | 0.018 | 0.0106 | 0.0164 | 0.0081 | 0.0051 | 0.0041 | 0.0057 | 0.0025 | 0.0001 | 0.0153 | 0.0079 | 0.0021 | 0.0139 | 0.0031 | 0.0005 | 0.0095 | 0.001 |
Mitochondria | 0.008 | 0.0003 | 0.0083 | 0.0018 | 0.0104 | 0.0036 | 0.0026 | 0.0127 | 0.0011 | 0.0001 | 0.0179 | 0.0021 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0068 | 0.0016 |
None | 0.0014 | 0.0003 | 0.0035 | 0.0004 | 0.0228 | 0.0004 | 0.0076 | 0.0001 | 0.0009 | 0 | 0.0036 | 0.0003 | 0.0015 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0159 | 0.0075 | 0.006 | 0.0251 | 0.0104 | 0.0235 | 0.014 | 0.0058 | 0.0046 | 0.0038 | 0.034 | 0.0037 | 0.0201 | 0.008 | 0.004 | 0.0034 | 0.0251 | 0.0064 |
Nucleolus | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0047 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0019 | 0.0006 | 0.0039 | 0.001 | 0.0046 | 0.0043 | 0.0019 | 0.0005 | 0.0023 | 0.0001 | 0.0079 | 0.0004 | 0.0012 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0003 |
Peroxisomes | 0.0018 | 0 | 0.0036 | 0.0003 | 0.0022 | 0.0011 | 0.0036 | 0 | 0.0003 | 0 | 0.0011 | 0.0019 | 0.0001 | 0 | 0 | 0 | 0.0274 | 0 |
Punctate Nuclear | 0.0035 | 0.0002 | 0.0016 | 0.0015 | 0.0086 | 0.0011 | 0.0024 | 0 | 0.0018 | 0 | 0.0041 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 |
Vacuole | 0.004 | 0.0049 | 0.0076 | 0.0027 | 0.0025 | 0.0021 | 0.0027 | 0.0056 | 0.0021 | 0.0006 | 0.0048 | 0.0019 | 0.0029 | 0.0025 | 0.0011 | 0.0005 | 0.0009 | 0.001 |
Vacuole Periphery | 0.0012 | 0.0003 | 0.0013 | 0.0012 | 0.0013 | 0.0012 | 0.0011 | 0.0072 | 0.0004 | 0.0004 | 0.005 | 0.0003 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0025 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 67.3039 | 44.0649 | 54.0776 | 56.4639 | 78.6591 | 68.214 | 56.1209 | 45.6847 | 51.3164 | 70.4555 |
Translational Efficiency | 2.5793 | 2.7197 | 2.0617 | 2.367 | 1.7247 | 2.3879 | 1.8094 | 2.3876 | 2.0159 | 2.0088 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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