Standard name | |
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Human Ortholog | |
Description | Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 10 | 2 | – | 11 | 5 | 16 | 13 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 2 | 2 | 13 | 13 | 18 | 26 | – | 14 | 22 | 14 | 11 | 10 | 29 | 57 | 57 | 22 | 14 | 0 | 0 | 0 | 2 | 2 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 1 |
Endosome | 0 | 0 | 1 | 1 | 2 | 0 | 3 | – | 1 | 2 | 1 | 1 | 0 | 2 | 1 | 3 | 1 | 3 | 2 | 1 | 0 | 0 | 7 | 8 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 4 | 3 |
Mitochondria | 10 | 22 | 2 | 4 | 2 | 65 | 23 | – | 66 | 39 | 35 | 48 | 4 | 5 | 2 | 4 | 3 | 5 | 0 | 2 | 0 | 1 | 4 | 6 |
Nucleus | 133 | 138 | 174 | 176 | 259 | 172 | 309 | – | 231 | 258 | 167 | 214 | 131 | 233 | 248 | 62 | 48 | 73 | 121 | 136 | 153 | 146 | 238 | 181 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 6 | 3 | 0 | 0 | 2 | 5 | 1 | – | 11 | 4 | 15 | 6 | 4 | 2 | 5 | 1 | 3 | 0 | 16 | 6 | 21 | 21 | 51 | 41 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 11 | 6 | 8 | 1 | 20 | – | 4 | 8 | 5 | 9 | 1 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 4 | 17 | 10 |
Vac/Vac Membrane | 3 | 4 | 1 | 2 | 5 | 1 | 6 | – | 1 | 6 | 4 | 1 | 1 | 2 | 5 | 7 | 5 | 5 | 7 | 6 | 5 | 11 | 25 | 35 |
Unique Cell Count | 151 | 153 | 178 | 193 | 281 | 211 | 361 | 276 | 310 | 212 | 247 | 145 | 265 | 313 | 124 | 75 | 96 | 160 | 157 | 186 | 193 | 368 | 306 | |
Labelled Cell Count | 160 | 171 | 191 | 204 | 294 | 273 | 392 | 344 | 349 | 264 | 309 | 152 | 278 | 320 | 141 | 87 | 105 | 160 | 157 | 186 | 193 | 368 | 306 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.0 | 5.9 | 6.3 | 6.9 | 4.4 | 5.9 | – | 5.4 | 5.4 | 5.6 | 5.6 | 5.9 | 6.0 | 6.2 | 8.1 | 7.9 | 7.5 | 5.3 | 5.9 | 5.7 |
Std Deviation (1e-4) | 0.9 | 0.7 | 0.9 | 1.6 | 1.6 | 1.4 | 1.8 | – | 1.6 | 1.2 | 1.6 | 1.5 | 1.0 | 1.6 | 1.7 | 2.3 | 2.1 | 1.8 | 1.3 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.23 | -0.41 | 0.01 | – | -0.12 | -0.11 | -0.07 | -0.07 | 0.01 | 0.03 | 0.09 | 0.46 | 0.43 | 0.35 | -0.14 | 0.0 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.7 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 2.1 | 3.3 | 3.0 | – | 2.2 | 2.9 | 2.7 | 2.0 | 2.7 | 4.0 | 5.6 | 9.7 | 7.0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.7 | – | 6.6 | 4.4 | 5.2 | 5.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.7 | -2.5 | -5.1 | -4.4 | – | -4.7 | -4.8 | -5.6 | -4.0 | -2.9 | -3.7 | -5.7 | -9.9 | -7.5 | -5.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.4 | -1.7 | 0 | -0.3 | – | -2.8 | -2.0 | -1.9 | -1.2 | -2.6 | -3.3 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1472 | 0.0866 | -0.0851 | -0.3649 | -0.4891 | -0.1666 | 1.223 | 1.4266 | 1.3631 | 1.381 | 1.0498 | 1.1248 | 3.0725 | 3.2768 | 2.9585 | 2.9417 | 2.8469 | 2.9633 |
Actin | 0.0088 | 0 | 0.004 | 0 | 0.0032 | 0.0009 | 0.0109 | 0.0003 | 0.0011 | 0.0012 | 0.0013 | 0 | 0.0153 | 0.0001 | 0 | 0 | 0.0015 | 0.0021 |
Bud | 0.0002 | 0 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0015 | 0.0001 | 0.0003 | 0.0022 | 0.0007 | 0.0008 | 0.0014 | 0.0002 | 0.0003 | 0.0006 | 0.0014 | 0.0019 | 0.01 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0013 |
Bud Periphery | 0.0007 | 0 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0008 | 0.0001 | 0.0019 | 0.0023 | 0.0006 | 0.0001 | 0.0015 | 0.0003 | 0.0009 | 0.0007 | 0.0022 | 0.0002 | 0.001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0007 | 0.0015 | 0.0006 | 0.006 | 0.0033 | 0.0002 | 0.01 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0049 | 0.0068 | 0.0001 | 0.0001 | 0.0056 | 0.0004 |
Cytoplasmic Foci | 0.0162 | 0 | 0.0019 | 0.0012 | 0.016 | 0.0005 | 0.0217 | 0.0003 | 0.0014 | 0.0236 | 0.0041 | 0.0012 | 0.0039 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0003 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.001 | 0.0004 | 0.0037 | 0.0001 | 0.0009 | 0.0008 | 0.0003 | 0.0001 | 0.0017 | 0.0001 | 0 | 0 | 0.0009 | 0.0002 |
Endosome | 0.0112 | 0.0002 | 0.0023 | 0.0029 | 0.0228 | 0.005 | 0.0127 | 0.0001 | 0.0175 | 0.037 | 0.0074 | 0.0061 | 0.0036 | 0.0001 | 0.0005 | 0 | 0.0029 | 0.0013 |
Golgi | 0.0031 | 0 | 0.0006 | 0.0001 | 0.0058 | 0.0013 | 0.006 | 0.0001 | 0.0048 | 0.0209 | 0.005 | 0.0005 | 0.0011 | 0 | 0.0003 | 0 | 0.0002 | 0.0004 |
Lipid Particles | 0.0223 | 0 | 0.0068 | 0.0001 | 0.0391 | 0.0032 | 0.0063 | 0.0001 | 0.007 | 0.0656 | 0.0397 | 0.0003 | 0.003 | 0 | 0.0068 | 0 | 0.0002 | 0.0009 |
Mitochondria | 0.0084 | 0.0001 | 0.0092 | 0.0007 | 0.0111 | 0.007 | 0.003 | 0.0089 | 0.0073 | 0.013 | 0.0172 | 0.0004 | 0.004 | 0.0001 | 0.0002 | 0 | 0.0011 | 0.0003 |
None | 0.002 | 0.0008 | 0.0004 | 0.0008 | 0.0176 | 0.0002 | 0.0047 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0161 | 0.0122 | 0.0001 | 0 | 0.0177 | 0.0023 |
Nuclear Periphery | 0.0078 | 0.0257 | 0.0096 | 0.0008 | 0.0145 | 0.0078 | 0.02 | 0.0022 | 0.0052 | 0.002 | 0.002 | 0.0038 | 0.0112 | 0.0012 | 0.0005 | 0.0022 | 0.0095 | 0.0014 |
Nucleolus | 0.0182 | 0.0148 | 0.0175 | 0.0173 | 0.0496 | 0.0468 | 0.0207 | 0.0167 | 0.0147 | 0.0052 | 0.0564 | 0.0647 | 0.0161 | 0.0201 | 0.0144 | 0.0056 | 0.0155 | 0.0367 |
Nucleus | 0.874 | 0.9511 | 0.9356 | 0.9462 | 0.7827 | 0.9159 | 0.8462 | 0.9557 | 0.929 | 0.8033 | 0.8488 | 0.912 | 0.8857 | 0.9537 | 0.9724 | 0.9915 | 0.8978 | 0.9453 |
Peroxisomes | 0.0074 | 0 | 0.0017 | 0.0001 | 0.017 | 0.003 | 0.0144 | 0.0002 | 0.0006 | 0.0168 | 0.0075 | 0.0001 | 0.0118 | 0 | 0.0012 | 0 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0109 | 0.0051 | 0.0038 | 0.0073 | 0.0125 | 0.005 | 0.0139 | 0.0139 | 0.0017 | 0.001 | 0.0021 | 0.0069 | 0.0076 | 0.0043 | 0.0029 | 0.0005 | 0.0447 | 0.0063 |
Vacuole | 0.0032 | 0.0001 | 0.0016 | 0.0097 | 0.0011 | 0.0008 | 0.0012 | 0.0001 | 0.0039 | 0.0057 | 0.0021 | 0.0008 | 0.001 | 0.0001 | 0 | 0 | 0.0006 | 0.0001 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.001 | 0.001 | 0.0009 | 0.0008 | 0.0007 | 0 | 0.0015 | 0.0016 | 0.0012 | 0.0005 | 0.0006 | 0 | 0 | 0 | 0.0006 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.0975 | 9.9205 | 5.2581 | 14.4566 | 9.2496 | 3.1689 | 15.7623 | 16.9249 | 15.1451 | 15.3399 |
Translational Efficiency | 2.7968 | 2.8812 | 4.3729 | 1.4389 | 2.6937 | 8.2445 | 1.4602 | 1.7216 | 1.7475 | 1.7907 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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