Standard name
Human Ortholog
Description Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.52 – 0.66 0.4 0.36 0.29 0.39 0.37 0.49 0.52 0.57 0.86 0.89 0.98 0.64 0.61 0.57 0.05 0.06 0.05 0.1 0.14 0.11
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0.05 – 0 0.14 0.26 0.22 0.25 0.28 0.17 0.16 0.09 0.11 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.4 0.57 – 0.34 0.67 0.67 0.66 0.63 0.6 0.57 0.54 0.58 0.14 0.14 0 0.5 0.49 0.49 0.86 0.84 0.87 0.83 0.77 0.78
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 1 0 0 0 0 0 0 1 1 0 1 0 0 0 0 1 1 0 0 3 3
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 110 85 – 61 76 62 95 135 49 102 92 171 89 221 346 213 224 156 14 10 2 9 34 31
Endoplasmic Reticulum 0 0 – 1 4 1 7 2 3 2 3 0 2 0 1 7 6 14 7 0 0 0 0 0
Endosome 13 7 – 0 3 2 0 3 0 0 0 0 1 0 0 8 6 5 11 9 0 1 5 6
Golgi 0 1 – 0 0 0 1 0 0 0 0 0 0 0 0 2 5 0 1 0 0 0 0 3
Mitochondria 25 1 – 0 1 2 11 6 5 10 13 9 0 0 1 8 9 4 2 3 0 0 4 5
Nucleus 20 9 – 4 26 45 71 88 37 36 29 28 11 4 1 16 22 14 1 1 0 1 2 3
Nuclear Periphery 4 1 – 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 8 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 0 1 1 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 2
Vac/Vac Membrane 87 93 – 31 128 114 215 217 79 117 96 174 15 34 15 166 181 133 272 149 36 71 191 217
Unique Cell Count 220 164 92 190 170 326 347 132 207 178 300 104 247 354 335 367 273 318 179 42 87 249 280
Labelled Cell Count 268 197 100 240 227 401 451 173 267 234 386 118 260 364 422 454 329 318 179 42 87 249 280


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.3 – 6.1 7.9 7.2 7.1 7.3 5.8 6.1 5.6 6.0 5.6 5.6 5.2 6.5 6.4 7.6 4.7 5.6 5.9
Std Deviation (1e-4) 0.7 0.9 – 1.6 1.6 1.5 1.5 1.7 1.4 1.5 1.4 1.4 1.1 1.6 1.1 1.4 1.6 4.8 0.7 1.3 2.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.562 -0.6683 -0.7141 -1.0266 -1.4666 -0.5924 -0.4617 -0.043 -0.1041 -0.2942 -0.3962 -0.2256 -1.1529 -1.0593 -1.2666 -0.763 -0.8083 -0.9172
Actin 0.0483 0.0055 0.0223 0.001 0.0064 0.0232 0.031 0.0001 0.0022 0.0074 0.0002 0.0035 0.0271 0.0003 0.0148 0.0028 0.0844 0.0014
Bud 0.001 0.0004 0.0006 0.0002 0.0004 0.0005 0.0013 0.0009 0.0036 0.0023 0.002 0.0006 0.0007 0.0005 0.0011 0.0007 0.0018 0.0005
Bud Neck 0.0038 0.0003 0.0011 0.0004 0.0019 0.003 0.0073 0.0018 0.0036 0.0036 0.0016 0.0033 0.0018 0.0006 0.0043 0.0067 0.0056 0.0039
Bud Periphery 0.0021 0.001 0.0008 0.0004 0.0003 0.0007 0.0019 0.0017 0.0038 0.0065 0.0026 0.001 0.0016 0.0006 0.0022 0.0008 0.0036 0.0009
Bud Site 0.0043 0.0013 0.0046 0.0002 0.002 0.0008 0.0075 0.0046 0.0134 0.0235 0.0048 0.0009 0.0055 0.0024 0.0073 0.0166 0.0054 0.0008
Cell Periphery 0.0007 0.0005 0.0004 0.0002 0.0001 0.0003 0.0016 0.0008 0.0006 0.0018 0.0007 0.0008 0.0007 0.0005 0.0007 0.0004 0.0008 0.0005
Cytoplasm 0.1005 0.27 0.1881 0.2182 0.2432 0.3033 0.1279 0.2275 0.1714 0.162 0.1007 0.2338 0.154 0.1863 0.1641 0.2862 0.0707 0.2863
Cytoplasmic Foci 0.0674 0.0486 0.0721 0.1037 0.0288 0.0707 0.0614 0.0444 0.0561 0.0829 0.0879 0.0635 0.0729 0.0546 0.0608 0.0634 0.0952 0.0567
Eisosomes 0.0013 0.0003 0.0002 0.0003 0.0039 0.0001 0.0006 0.0001 0.0002 0.0003 0.0001 0.0001 0.0006 0.0001 0.0003 0.0002 0.0002 0
Endoplasmic Reticulum 0.0075 0.0048 0.0067 0.0043 0.005 0.0039 0.0076 0.0064 0.0038 0.0038 0.002 0.0053 0.005 0.0028 0.0034 0.0045 0.0036 0.0041
Endosome 0.1323 0.0786 0.1562 0.2428 0.2037 0.1624 0.0831 0.0811 0.0935 0.1247 0.2081 0.1473 0.1127 0.0913 0.1467 0.2095 0.192 0.1283
Golgi 0.0143 0.0011 0.0045 0.0016 0.0018 0.0094 0.012 0.0011 0.0052 0.0099 0.0079 0.0053 0.0153 0.0012 0.0054 0.0023 0.0186 0.0058
Lipid Particles 0.0332 0.0056 0.0123 0.0031 0.0035 0.0105 0.0472 0.0046 0.0111 0.0169 0.0324 0.0063 0.0344 0.0064 0.0103 0.0033 0.0268 0.0055
Mitochondria 0.0069 0.0017 0.0035 0.0017 0.0016 0.0115 0.0163 0.0033 0.0115 0.0436 0.0177 0.0028 0.0119 0.0015 0.0054 0.0024 0.0151 0.0044
None 0.4838 0.449 0.389 0.2972 0.2303 0.2058 0.4806 0.4871 0.5296 0.3134 0.3139 0.291 0.4169 0.4954 0.442 0.2275 0.3092 0.2733
Nuclear Periphery 0.0151 0.0025 0.0051 0.0042 0.0088 0.0032 0.0105 0.0037 0.002 0.0044 0.0018 0.0038 0.0054 0.0025 0.0033 0.0105 0.0032 0.0046
Nucleolus 0.0014 0.0011 0.0013 0.0009 0.0011 0.0013 0.003 0.0023 0.0024 0.0116 0.0048 0.0019 0.0025 0.0022 0.002 0.0011 0.0029 0.0033
Nucleus 0.0026 0.0033 0.0053 0.0029 0.0263 0.0209 0.0049 0.0088 0.0053 0.0164 0.0042 0.0131 0.006 0.0066 0.0039 0.0109 0.0054 0.0126
Peroxisomes 0.0231 0.0005 0.0152 0.0008 0.0023 0.0081 0.0183 0.0005 0.0063 0.0118 0.0049 0.0016 0.0179 0.0006 0.0033 0.001 0.0357 0.0013
Punctate Nuclear 0.0075 0.0019 0.0034 0.0013 0.0036 0.0174 0.0109 0.0012 0.0024 0.0061 0.0092 0.0027 0.0116 0.0016 0.0028 0.0018 0.005 0.0019
Vacuole 0.0364 0.1167 0.0976 0.1049 0.2012 0.1319 0.0605 0.1119 0.067 0.1308 0.1744 0.199 0.0883 0.1342 0.105 0.1245 0.1046 0.1903
Vacuole Periphery 0.0065 0.0054 0.0096 0.01 0.0239 0.011 0.0046 0.0058 0.0049 0.0163 0.018 0.0124 0.0072 0.0079 0.0108 0.0232 0.0103 0.0134

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6326 3.9793 15.5024 16.5219 12.2645 24.4694 14.4594 19.0091 20.1996 16.6846
Translational Efficiency 1.4937 3.5357 0.9496 1.3131 1.1325 1.1435 1.1761 1.0562 1.2008 1.0204

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation
Localization
Cell Percentages vacuole (79%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ecm38

Ecm38


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm38-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available