Standard name
Human Ortholog
Description Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.87 0.93 0.8 0.74 0.65 0.58 0.47 0.4 0.39 0.36 0.42 0.34 0.91 0.9 0.95 0.9 0.86 0.88 0.79 0.84 0.65 0.72 0.69 0.67
Endoplasmic Reticulum 0.09 0.05 0.08 0.07 0.07 0.05 0 0.06 0 0 0.05 0 0.1 0.08 0 0.06 0 0 0.05 0 0.12 0.09 0.11 0.12
Endosome 0 0 0 0.06 0.09 0.12 0.11 0.12 0.1 0.09 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0.11 0.1 0.31 0.37 0.4 0.54 0.52 0.53 0.62 0 0 0 0.06 0.08 0.06 0.05 0 0.09 0.08 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.08 0.07 0.05 0.09 0 0.05 0 0 0 0 0.07 0 0 0.05 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 0 11 15 28 12 14 40 16 19 21 25 3 3 0 2 4 5 1 0 6 0 2 2
Bud 0 4 1 4 5 9 13 24 26 17 28 27 2 0 0 1 2 0 1 0 2 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 4 1 3 4
Bud Site 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0
Cell Periphery 4 1 11 11 8 14 16 15 21 13 24 20 7 4 2 11 18 11 1 0 1 0 0 0
Cytoplasm 177 135 245 199 287 276 260 294 198 233 243 234 147 187 236 186 452 187 185 140 236 181 326 310
Endoplasmic Reticulum 19 7 25 19 33 23 22 42 21 27 28 24 16 17 9 12 23 7 11 4 42 23 53 53
Endosome 1 0 10 16 41 55 59 87 50 55 43 45 0 1 2 5 16 6 6 1 12 1 8 13
Golgi 0 0 2 9 30 13 5 9 2 6 9 3 0 0 0 0 3 0 3 1 10 3 9 16
Mitochondria 2 11 13 29 44 145 208 293 274 334 311 433 0 0 0 13 41 13 10 4 31 20 29 26
Nucleus 1 0 1 2 1 0 4 7 1 2 2 0 2 1 1 1 4 3 0 1 0 1 0 0
Nuclear Periphery 0 1 0 0 1 0 1 2 1 4 0 1 0 0 0 0 0 0 0 0 1 0 2 0
Nucleolus 0 0 0 0 0 0 1 1 0 4 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 3 1 0 2 3 4 4 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 0 0 0 1 0 4 10 3 2 2 3 0 0 0 0 1 0 1 0 1 0 1 1
Vac/Vac Membrane 2 0 9 12 22 21 42 52 27 55 26 33 0 2 3 6 36 9 3 8 7 14 27 25
Unique Cell Count 204 145 307 268 443 475 558 737 507 641 585 695 162 207 249 207 525 212 235 166 364 251 473 464
Labelled Cell Count 215 159 328 316 501 571 651 876 642 775 744 855 177 215 253 237 600 241 235 166 364 251 473 464


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.0 8.5 9.0 8.5 7.4 6.6 6.9 6.5 6.2 6.4 5.9 8.9 9.0 8.9 6.6 7.9 9.3 9.9 10.1 10.2
Std Deviation (1e-4) 1.2 1.0 1.3 1.6 1.4 1.4 1.4 1.4 1.5 1.3 1.5 1.2 0.9 1.0 1.0 1.1 1.4 1.4 1.2 1.4 1.5
Intensity Change (Log2) 0.08 -0.0 -0.19 -0.37 -0.3 -0.4 -0.45 -0.42 -0.52 0.07 0.09 0.06 -0.36 -0.11 0.12 0.21 0.24 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 1.7 -0.9 -0.9 1.3 -0.3 -0.5 0 0 0 -1.5 0 -1.9 -3.0 -0.8
Bud 0 0 0 0 2.8 3.7 2.5 3.6 3.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -1.5 -0.5 -0.6 -1.5 0.4 -1.4 0.4 -0.6 0.4 -1.1 -2.2 1.0 -0.1 0.9
Cytoplasm -1.6 -4.5 -6.3 -9.5 -11.8 -11.3 -12.5 -10.9 -13.5 3.0 3.2 5.1 3.0 2.4 2.5
Endoplasmic Reticulum -0.5 -0.3 -1.9 -2.6 -1.5 -2.4 -2.5 -2.0 -3.2 0.6 0 -2.2 -1.0 -2.2 -2.3
Endosome 1.6 3.2 4.1 3.8 4.3 3.5 3.0 2.5 2.1 0 0 -2.0 -0.6 -0.2 -0.3
Golgi 2.4 4.1 2.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 2.9 8.9 10.7 11.5 14.4 14.3 14.4 17.1 0 -3.0 -3.3 1.0 2.0 1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 1.4 1.1 2.7 2.6 1.6 3.2 1.1 1.3 0 0 -1.4 0 2.4 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.797 6.6139 5.996 5.136 5.9489 5.8242 3.7455 5.0339 4.4487 4.1486 4.0277 4.5397 2.9027 4.6776 4.214 4.2018 3.0202 4.1813
Actin 0.0146 0.0082 0.0058 0.0262 0.0008 0.003 0.0018 0.0021 0.0253 0.0136 0.0437 0.0028 0.0381 0.0576 0.0178 0.0031 0.0113 0.0014
Bud 0.0009 0.0009 0.0009 0.0012 0.0005 0.0005 0.0002 0.0005 0.0009 0.0008 0.0004 0.0003 0.0012 0.0015 0.0011 0.0016 0.0009 0.0003
Bud Neck 0.0011 0.0008 0.0006 0.0036 0.0005 0.002 0.0002 0.0002 0.0011 0.0004 0.0006 0.0009 0.0008 0.0004 0.001 0.0005 0.0007 0.0011
Bud Periphery 0.0006 0.0003 0.0004 0.0009 0.0001 0.0003 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0009 0.0005 0.0004 0.0006 0.0008 0.0002
Bud Site 0.0048 0.0074 0.0028 0.0057 0.0004 0.0003 0.0022 0.0017 0.0041 0.0006 0.001 0.0002 0.001 0.0116 0.0037 0.0015 0.0011 0.0002
Cell Periphery 0.0005 0.0003 0.0003 0.0006 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.5209 0.6801 0.6169 0.509 0.4695 0.5796 0.7542 0.6752 0.6398 0.5718 0.5161 0.6286 0.5044 0.774 0.5828 0.4583 0.4033 0.6757
Cytoplasmic Foci 0.0842 0.0795 0.0799 0.06 0.1329 0.0878 0.0446 0.0326 0.0909 0.0623 0.1093 0.0933 0.0445 0.079 0.0745 0.1035 0.1132 0.0622
Eisosomes 0.0003 0 0.0001 0.0007 0 0.0001 0 0 0 0 0.0002 0 0.0002 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0201 0.009 0.0089 0.0092 0.0074 0.013 0.0275 0.0078 0.0097 0.0028 0.0107 0.0102 0.0131 0.0022 0.0077 0.008 0.0034 0.0149
Endosome 0.2718 0.1624 0.2219 0.2873 0.3253 0.238 0.1345 0.1151 0.1601 0.2587 0.2207 0.211 0.1537 0.0404 0.2201 0.3266 0.3127 0.1655
Golgi 0.0439 0.0256 0.0337 0.0355 0.0429 0.0413 0.0066 0.0287 0.0382 0.0665 0.0643 0.0392 0.0702 0.0106 0.0497 0.0417 0.054 0.0286
Lipid Particles 0.0019 0.0031 0.0005 0.0011 0.0003 0.0005 0.0012 0.0003 0.0014 0.0001 0.0074 0.0002 0.0029 0.0018 0.0029 0.0005 0.0232 0.005
Mitochondria 0.0083 0.005 0.0077 0.0145 0.005 0.022 0.0013 0.0059 0.0029 0.0019 0.0057 0.0049 0.0256 0.0018 0.0065 0.0151 0.0162 0.0195
None 0.0049 0.002 0.0021 0.001 0.0009 0.0012 0.0069 0.1198 0.0021 0.0009 0.0015 0.001 0.0294 0.0023 0.0154 0.001 0.0007 0.0014
Nuclear Periphery 0.003 0.0009 0.0013 0.0019 0.0007 0.0012 0.0038 0.0008 0.0012 0.0007 0.0014 0.0005 0.0449 0.0007 0.0015 0.0018 0.0026 0.001
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0.0001 0 0.0008 0.0001 0.0001 0 0.0001 0
Nucleus 0.0012 0.001 0.0009 0.0011 0.0004 0.0006 0.0012 0.0005 0.0007 0.0005 0.0006 0.0004 0.0114 0.0007 0.0008 0.0008 0.0009 0.0006
Peroxisomes 0.0017 0.0017 0.001 0.0018 0.0017 0.0007 0.0003 0.0001 0.0094 0.0006 0.0073 0.001 0.0026 0.0087 0.0021 0.0058 0.0338 0.0155
Punctate Nuclear 0.0005 0.0003 0.0004 0.0002 0.0002 0.0002 0.0006 0.0002 0.0004 0.0001 0.0015 0.0002 0.0471 0.0006 0.0004 0.0004 0.001 0.0005
Vacuole 0.0109 0.0085 0.0094 0.0302 0.0064 0.0045 0.0107 0.006 0.0084 0.0118 0.0039 0.0032 0.004 0.0044 0.0085 0.0201 0.0141 0.0036
Vacuole Periphery 0.0041 0.0028 0.0045 0.0084 0.0039 0.0033 0.0019 0.002 0.0029 0.0057 0.0034 0.0021 0.0032 0.0011 0.0028 0.0088 0.0057 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7284 18.0259 19.5289 26.7574 20.9154 14.5104 20.4131 24.9716 25.4921 23.6279
Translational Efficiency 1.7606 1.7783 1.4653 1.3253 1.0819 1.8205 1.344 1.1094 1.0691 1.0167

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Imh1

Imh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Imh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available