Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog; hSNF5 tumor suppressor ortholog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.08 0.14 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.05 0.09 0 0.08 0.08 0.11 0.14 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.93 0.91 0.81 0.82 0.77 0.73 0.7 0.74 0.69 0.68 0.59 0.59 0.83 0.87 0.78 – – – 0.75 0.79 0.6 0.79 0.79 0.76
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.29 0.54 0.4 0.42 0.35 0.47 0.5 0.47 0.54 0.51 0.59 0.55 0.3 0.15 0.17 – – – 0.18 0.18 0.34 0.16 0.13 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 1 1 4 1 0 0 0 1 – – – 1 0 0 1 3 6
Bud Neck 0 0 0 1 0 0 2 4 0 3 2 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 1 1 0 1 0 3 8 13 13 15 11 11 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0
Cytoplasm 3 0 4 4 11 8 7 2 5 5 9 5 12 32 63 – – – 0 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 5 2 1 – – – 1 0 0 2 5 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 1
Mitochondria 1 7 0 1 4 12 25 12 24 21 25 34 0 0 0 – – – 0 0 1 0 0 2
Nucleus 167 104 144 130 126 162 202 220 204 185 132 140 234 360 360 – – – 139 93 117 219 367 368
Nuclear Periphery 0 0 0 0 0 3 2 2 3 1 3 1 0 0 1 – – – 0 0 0 0 1 0
Nucleolus 52 61 72 67 57 104 144 138 160 139 132 130 84 64 79 – – – 34 20 67 45 62 68
Peroxisomes 1 0 0 0 0 0 0 1 0 0 0 0 1 1 3 – – – 0 0 1 0 0 0
SpindlePole 0 1 0 0 1 1 0 1 0 1 0 0 2 2 7 – – – 2 0 1 2 3 4
Vac/Vac Membrane 0 0 1 1 1 0 0 4 5 2 2 2 2 7 6 – – – 4 3 3 3 14 18
Unique Cell Count 180 114 178 159 164 223 289 296 295 274 223 236 282 415 460 187 119 196 278 464 483
Labelled Cell Count 225 174 222 205 200 294 392 398 415 376 317 323 340 469 521 187 119 196 278 464 483


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 12.7 12.7 10.5 10.1 8.8 8.5 9.3 8.4 8.4 7.7 7.8 12.8 12.6 12.3 – – – 12.8 13.2 13.8
Std Deviation (1e-4) 2.6 4.2 4.5 2.9 4.3 3.4 3.1 3.6 2.9 3.5 3.2 3.0 3.4 3.7 4.4 – – – 2.9 3.8 3.0
Intensity Change (Log2) – – – -0.28 -0.33 -0.53 -0.58 -0.45 -0.59 -0.59 -0.71 -0.7 0.02 -0.0 -0.05 – – – 0.02 0.06 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 3.2 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 2.0 0.8 0 0 0 0 1.0 0 1.1 2.6 4.2 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 3.1 4.0 0 3.9 3.8 4.6 5.3 0 0 0 – – –
Nucleus 0.2 -0.9 -1.9 -2.6 -1.6 -2.8 -3.1 -4.7 -4.7 0.6 1.8 -0.7 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0.3 -1.1 1.2 2.0 1.3 2.9 2.1 3.7 2.9 -2.4 -6.6 -6.2 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8715 11.9783 11.4223 10.2775 8.6246 10.2355 9.2723 7.5727 7.1043 8.6826 7.3855 7.927 8.2643 8.8627 8.0386 7.5494 7.6477 7.2947
Actin 0.0004 0.0003 0.0115 0 0 0 0.009 0.0007 0 0.0004 0 0.0074 0.0221 0.0001 0.0031 0.0004 0.0011 0
Bud 0 0 0.0001 0 0 0 0.0016 0.0008 0 0.0007 0 0.0005 0.0014 0 0.0005 0.0001 0.0009 0.0001
Bud Neck 0.0001 0 0.0001 0 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0012 0.0057 0 0.0013 0.0006 0.0008 0.0003
Bud Periphery 0 0.0002 0.0001 0 0.0001 0 0.0018 0.0007 0 0.0004 0 0.0014 0.0016 0 0.0007 0.0002 0.001 0.0001
Bud Site 0 0.0001 0.0009 0 0 0 0.0024 0.0012 0 0.0022 0 0.0006 0.0068 0.0001 0.0027 0.0016 0.0043 0.0001
Cell Periphery 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0.0009 0.0005 0 0.0004 0.0002 0.0007 0
Cytoplasm 0.0023 0.0001 0.0009 0 0 0 0.0012 0.0002 0 0.0002 0 0.008 0.0066 0.0004 0.0009 0.0004 0.0132 0.0087
Cytoplasmic Foci 0.0065 0 0.0036 0 0 0 0.0024 0.0008 0 0.0059 0 0.0023 0.0421 0.0015 0.0062 0.0262 0.0194 0
Eisosomes 0.0001 0.0003 0.0001 0 0 0 0.0001 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0005 0
Endoplasmic Reticulum 0 0.0001 0.0015 0 0 0 0.0005 0 0 0 0 0.0041 0.0023 0.0001 0.0006 0.0001 0.0023 0.0002
Endosome 0.001 0.0002 0.0012 0 0 0 0.0041 0.0001 0 0.0003 0 0.0079 0.0268 0.0001 0.0048 0.0189 0.0736 0.0001
Golgi 0.0002 0.0001 0.0009 0 0 0 0.0019 0.0001 0 0.0004 0 0.0039 0.0106 0.0001 0.0044 0.0034 0.0056 0
Lipid Particles 0.0015 0.0001 0.0026 0 0 0 0.0014 0.0001 0 0.0005 0 0.0067 0.0216 0.0007 0.0045 0.0066 0.005 0
Mitochondria 0.0001 0.0459 0.0005 0.0001 0.0004 0.0001 0.0141 0.0005 0.0001 0.001 0.0005 0.0068 0.0126 0.0008 0.0084 0.0007 0.0363 0.0013
None 0.0407 0.0002 0.0013 0 0 0 0.0009 0.0001 0 0.0004 0 0.0006 0.0049 0.0012 0.0006 0.0001 0.0184 0.0002
Nuclear Periphery 0.0019 0.0006 0.0008 0.0001 0.0036 0 0.0133 0.0005 0.0001 0.0011 0.0001 0.0177 0.004 0.0009 0.0086 0.0001 0.0054 0.0006
Nucleolus 0.0223 0.0407 0.0162 0.0222 0.1916 0.1574 0.0541 0.0411 0.017 0.0263 0.3568 0.1971 0.0608 0.0395 0.033 0.0464 0.3459 0.1832
Nucleus 0.8378 0.9095 0.9559 0.9774 0.7443 0.842 0.8792 0.9468 0.9826 0.9041 0.6417 0.7205 0.7331 0.9514 0.9115 0.8713 0.4449 0.8043
Peroxisomes 0.0005 0.0001 0.0004 0 0 0 0.001 0.0005 0 0.0011 0 0.0009 0.0162 0.0001 0.0008 0.0127 0.0018 0
Punctate Nuclear 0.0845 0.0005 0.0011 0.0002 0.0597 0.0001 0.0075 0.0054 0 0.0546 0.0006 0.0037 0.0136 0.0024 0.0037 0.0018 0.0055 0.0005
Vacuole 0.0001 0.0006 0.0001 0 0.0001 0.0001 0.0013 0.0001 0 0.0001 0 0.0044 0.0038 0.0001 0.0015 0.0074 0.01 0.0001
Vacuole Periphery 0 0.0002 0.0001 0 0.0001 0 0.0018 0.0001 0 0.0002 0.0001 0.0032 0.0026 0.0002 0.0015 0.0007 0.0033 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.6727 14.6301 18.8554 22.674 18.9855 12.9133 19.2691 25.748 19.0632 25.1411
Translational Efficiency 2.3457 3.2474 2.2049 1.7064 2.3796 3.7204 2.4438 1.5887 1.953 1.9754

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog; hSNF5 tumor suppressor ortholog
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Sfh1

Sfh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available