Standard name
Human Ortholog
Description Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.92 0.88 0.81 0.45 0.59 0.38 0.31 0.34 0.36 0.26 0.31 0.93 0.88 0.78 0.75 0.68 0.72 0.72 0.67 0.8 0.79 0.67 0.59
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.14 0.24 0.21 0.31 0.37 0.47 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.07 0.09 0.09 0.1 0.15 0.11 0.14 0 0 0 0 0.05 0 0.14 0.2 0.08 0.11 0.2 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.2 0.32 0.57 0.34 0.42 0.49 0.43 0.27 0.39 0.31 0.1 0.2 0.37 0.36 0.44 0.39 0 0 0.06 0 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 1 0 1 0 8 5 4 7 10 4 0 0 0 0 0 0 0 5 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 1 3 3 0 2 2 0 0 0 5 0 0 0 0 0 0 0 0
Cytoplasm 87 154 243 175 134 191 195 158 136 176 90 127 38 126 145 282 206 132 73 116 223 21 109 77
Endoplasmic Reticulum 0 4 4 0 1 1 2 1 0 10 0 5 1 0 0 9 11 2 1 3 6 0 0 1
Endosome 0 0 0 0 10 3 14 16 3 1 1 0 0 1 0 0 0 0 0 1 2 0 1 4
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 3 4 2 1 0 0 0 0 0 0
Mitochondria 2 0 1 4 14 46 120 106 123 180 164 175 0 0 1 1 3 1 2 0 1 0 0 0
Nucleus 1 4 1 1 6 23 48 46 41 72 39 59 0 2 1 10 15 3 14 35 21 2 32 28
Nuclear Periphery 0 1 0 0 0 0 2 1 1 1 1 1 0 0 0 0 0 0 0 2 2 0 0 1
Nucleolus 0 0 0 0 1 0 2 11 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Vac/Vac Membrane 12 16 56 69 170 110 212 247 170 132 134 127 4 29 70 136 132 72 4 6 17 0 12 8
Unique Cell Count 95 168 277 216 296 326 507 506 398 493 347 413 41 144 187 377 302 183 102 175 281 27 163 132
Labelled Cell Count 102 180 306 250 337 375 604 594 482 581 442 500 43 158 220 447 369 212 102 175 281 27 163 132


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 11.6 11.2 9.9 9.2 7.9 7.3 6.8 6.6 6.4 5.8 5.9 11.9 12.9 12.1 13.2 12.6 13.2 – 12.2 12.7
Std Deviation (1e-4) 1.6 2.0 1.2 1.5 1.4 1.6 1.5 1.3 1.3 1.1 1.2 1.2 2.1 1.6 1.8 2.1 2.2 2.3 – 1.8 1.8
Intensity Change (Log2) – – – -0.17 -0.28 -0.51 -0.62 -0.72 -0.76 -0.8 -0.94 -0.92 0.09 0.21 0.12 0.24 0.17 0.24 – 0.13 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.1 -10.7 -7.9 -13.3 -15.1 -13.8 -13.9 -15.4 -14.7 0.9 -0.1 -2.9 -4.1 -5.6 -4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0.6 0 0 0 0 0 0.9 1.7 0
Endosome 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.3 6.3 8.6 8.0 10.1 11.4 13.2 12.4 0 0 0 0 0 0
Nucleus 0 0 4.2 5.0 4.9 5.3 6.5 5.5 6.4 0 0 0 0 3.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 9.1 3.7 6.1 7.9 6.1 2.0 5.0 3.1 -1.6 0 4.1 4.4 6.0 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6343 9.5287 8.5028 8.4507 6.8543 8.3508 6.2239 9.9349 9.4501 7.2508 8.0799 8.7253 8.1216 10.4233 9.4185 8.8124 8.9302 9.4633
Actin 0.0051 0.0002 0.0002 0.0002 0.0229 0.0022 0.0728 0.0003 0.0022 0.0507 0.0038 0.0014 0.008 0.0002 0.0015 0.0209 0.0011 0.0002
Bud 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0032 0.0001 0.0026 0.0008 0.0002 0.0003 0.0007 0.0001 0.0001 0.0008 0.0011 0.0001
Bud Neck 0.0024 0.0003 0.0006 0.0006 0.0022 0.0017 0.0019 0.0003 0.0004 0.0017 0.0007 0.0017 0.0006 0.0002 0.0004 0.001 0.001 0.001
Bud Periphery 0.0001 0 0 0 0.0002 0.0001 0.0026 0 0.0006 0.001 0.0001 0.0001 0.0009 0 0 0.0005 0.0013 0
Bud Site 0.0019 0.0002 0.0001 0.0001 0.0006 0.0002 0.0071 0.0003 0.0008 0.005 0.0002 0.0002 0.0019 0.0001 0.0002 0.0015 0.0004 0.0001
Cell Periphery 0.0001 0 0 0 0.0002 0.0001 0.0008 0 0 0.0005 0.0001 0 0.0001 0 0 0.0002 0.0001 0
Cytoplasm 0.4546 0.5902 0.4479 0.5312 0.4137 0.5341 0.4398 0.7121 0.5816 0.341 0.798 0.541 0.4909 0.7302 0.603 0.4315 0.6097 0.5457
Cytoplasmic Foci 0.0068 0.0008 0.0011 0.0023 0.0041 0.0017 0.0314 0.0034 0.0033 0.0151 0.0061 0.0062 0.0065 0.0122 0.0179 0.0045 0.0008 0.0011
Eisosomes 0.0001 0 0 0 0.0005 0 0.0007 0 0 0.0026 0.0001 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0043 0.0018 0.0018 0.0085 0.0088 0.005 0.0118 0.0009 0.0018 0.0049 0.01 0.0033 0.0035 0.0007 0.0019 0.0024 0.0017 0.0018
Endosome 0.0091 0.0012 0.0011 0.0095 0.0144 0.0075 0.0581 0.0008 0.0039 0.0479 0.0209 0.0082 0.011 0.0004 0.0083 0.0122 0.0028 0.0065
Golgi 0.002 0 0 0.0001 0.0033 0.0006 0.0323 0.0001 0.0002 0.0362 0.0029 0.0021 0.0033 0 0.0011 0.0025 0.0001 0.0002
Lipid Particles 0.0016 0 0 0 0.0039 0.0002 0.0136 0.0005 0.0003 0.033 0.0182 0.001 0.005 0.0002 0.0017 0.003 0.0002 0
Mitochondria 0.0029 0.0003 0.0003 0.0003 0.0009 0.0009 0.0348 0.0004 0.008 0.0213 0.0032 0.0008 0.0158 0.0002 0.0004 0.0025 0.0036 0.0003
None 0.0015 0.0007 0.0006 0.0035 0.0069 0.0008 0.011 0.0021 0.0019 0.0009 0.003 0.0006 0.0029 0.0006 0.0013 0.006 0.0011 0.0005
Nuclear Periphery 0.029 0.0177 0.0178 0.0244 0.0408 0.0251 0.0266 0.0101 0.0154 0.0219 0.0276 0.0289 0.0272 0.0105 0.0115 0.022 0.0244 0.0192
Nucleolus 0.0006 0.0003 0.0002 0.0003 0.0025 0.0003 0.0052 0.0018 0.0012 0.0003 0.0002 0.0003 0.0008 0.0001 0.0002 0.0018 0.0025 0.0004
Nucleus 0.4676 0.3825 0.5235 0.4124 0.4518 0.4133 0.2081 0.2623 0.3687 0.3992 0.0978 0.3946 0.4105 0.2426 0.3324 0.4752 0.336 0.4189
Peroxisomes 0.001 0 0.0001 0 0.0004 0.0001 0.0077 0.0001 0.0004 0.0073 0.0005 0.0007 0.0025 0.0001 0.0017 0.0008 0.0001 0
Punctate Nuclear 0.0064 0.002 0.0035 0.0036 0.0176 0.0022 0.0211 0.0037 0.0047 0.0045 0.0027 0.0049 0.0039 0.0009 0.0146 0.0068 0.0024 0.0021
Vacuole 0.0022 0.0012 0.0007 0.0024 0.003 0.0027 0.0065 0.0005 0.0013 0.0024 0.0022 0.0028 0.0025 0.0005 0.0012 0.0028 0.0061 0.0013
Vacuole Periphery 0.0007 0.0003 0.0002 0.0006 0.0011 0.0009 0.0032 0.0003 0.0005 0.0018 0.0016 0.0007 0.0012 0.0002 0.0003 0.0009 0.0035 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1605 31.8977 26.0971 34.5644 24.9112 37.4913 41.5552 36.2796 38.021 37.795
Translational Efficiency 0.9378 1.031 1.0215 0.759 0.9887 0.8917 1.0425 1.1371 1.002 1.0639

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nma1

Nma1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nma1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available