Standard name | |
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Human Ortholog | |
Description | Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 |
Bud | 0 | 0 | 0 | 2 | 0 | 1 | 4 | – | 0 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 2 | 2 | 1 | 6 | 1 | 3 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 8 | 1 | 8 | 10 | 8 | 4 | – | 3 | 5 | 4 | 6 | 9 | 27 | 40 | 32 | 21 | 21 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 3 | 4 | 4 | 0 | 3 | – | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 6 | 9 | 7 | 0 | 0 | 1 | 2 | 2 | 0 |
Endosome | 0 | 0 | 3 | 1 | 3 | 10 | 14 | – | 5 | 2 | 2 | 21 | 1 | 3 | 13 | 3 | 5 | 3 | 0 | 1 | 1 | 4 | 12 | 18 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 2 | 0 | 0 | 0 | 2 | 5 | 7 |
Mitochondria | 11 | 14 | 4 | 5 | 13 | 51 | 74 | – | 113 | 120 | 154 | 83 | 29 | 32 | 38 | 28 | 11 | 17 | 2 | 5 | 11 | 25 | 57 | 58 |
Nucleus | 17 | 19 | 15 | 19 | 17 | 18 | 30 | – | 25 | 29 | 26 | 29 | 12 | 6 | 6 | 4 | 2 | 10 | 6 | 4 | 4 | 0 | 1 | 1 |
Nuclear Periphery | 126 | 194 | 310 | 251 | 255 | 290 | 262 | – | 206 | 194 | 168 | 220 | 433 | 334 | 393 | 171 | 148 | 124 | 155 | 271 | 380 | 257 | 319 | 290 |
Nucleolus | 39 | 52 | 29 | 38 | 45 | 93 | 131 | – | 121 | 137 | 159 | 154 | 125 | 102 | 151 | 47 | 51 | 40 | 10 | 21 | 4 | 7 | 5 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 12 | 9 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 3 | 2 | – | 1 | 2 | 3 | 1 | 2 | 7 | 15 | 1 | 2 | 2 | 1 | 1 | 1 | 0 | 1 | 2 |
Vac/Vac Membrane | 33 | 77 | 87 | 30 | 44 | 48 | 32 | – | 30 | 25 | 27 | 29 | 41 | 47 | 59 | 91 | 99 | 68 | 8 | 28 | 10 | 11 | 9 | 10 |
Unique Cell Count | 172 | 288 | 367 | 299 | 334 | 406 | 421 | 372 | 365 | 398 | 406 | 485 | 421 | 532 | 284 | 248 | 210 | 188 | 343 | 419 | 322 | 440 | 415 | |
Labelled Cell Count | 227 | 365 | 453 | 361 | 394 | 524 | 558 | 508 | 519 | 548 | 556 | 653 | 567 | 725 | 385 | 353 | 294 | 188 | 343 | 419 | 322 | 440 | 415 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.7 | 18.2 | 15.0 | 13.6 | 13.8 | 11.1 | 11.0 | – | 9.3 | 8.4 | 8.1 | 9.3 | 15.1 | 13.9 | 13.8 | 21.9 | 23.5 | 20.4 | 13.8 | 13.4 | 13.8 |
Std Deviation (1e-4) | 3.8 | 5.3 | 3.6 | 3.7 | 3.8 | 2.7 | 2.8 | – | 2.5 | 2.1 | 2.1 | 2.8 | 3.9 | 4.3 | 4.6 | 7.8 | 8.0 | 7.8 | 3.6 | 3.1 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.12 | -0.43 | -0.45 | – | -0.69 | -0.84 | -0.9 | -0.69 | 0.01 | -0.11 | -0.12 | 0.55 | 0.64 | 0.44 | -0.12 | -0.16 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.6 | 5.1 | 6.3 | 5.4 | 5.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1.8 | 2.4 | – | 0 | 0 | 0 | 3.5 | 0 | 0 | 1.8 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.4 | 6.2 | 7.7 | – | 10.9 | 11.5 | 12.8 | 8.5 | 3.7 | 4.4 | 4.2 | 5.1 | 2.6 | 4.3 |
Nucleus | 1.3 | 0.6 | 0.2 | 1.8 | – | 1.6 | 2.2 | 1.5 | 1.8 | -1.3 | -2.3 | -2.9 | -2.0 | -2.4 | 0.4 |
Nuclear Periphery | -0.2 | -2.7 | -4.3 | -7.0 | – | -8.6 | -9.1 | -12.1 | -9.1 | 2.1 | -1.9 | -3.8 | -7.0 | -6.9 | -6.8 |
Nucleolus | 2.1 | 2.4 | 5.7 | 8.1 | – | 8.3 | 9.6 | 10.3 | 9.8 | 6.7 | 6.1 | 7.5 | 3.4 | 4.6 | 4.0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Vacuole | -4.6 | -3.6 | -4.3 | -6.3 | – | -5.8 | -6.3 | -6.6 | -6.4 | -6.2 | -4.7 | -5.0 | 2.4 | 4.3 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.9471 | 13.9696 | 12.3189 | 12.0579 | 10.8741 | 13.4207 | 18.0748 | 17.7718 | 16.8109 | 16.4652 | 16.3817 | 16.6037 | 19.3367 | 16.9161 | 16.6183 | 15.2905 | 16.9491 | 15.716 |
Actin | 0.0083 | 0.0001 | 0.0056 | 0.0081 | 0.0436 | 0.0015 | 0.0209 | 0.0002 | 0.0039 | 0.0022 | 0.0024 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0015 | 0.0003 | 0.0001 |
Bud | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Bud Periphery | 0.0002 | 0 | 0.0006 | 0.0009 | 0.0008 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0013 | 0 | 0.0002 | 0.0003 | 0.002 | 0.0001 | 0.002 | 0 | 0.0012 | 0.0003 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0018 | 0.0006 | 0.0003 | 0.004 | 0.0042 | 0.0123 | 0.0125 | 0.0002 | 0.0085 | 0.0001 | 0.0023 | 0.0033 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0181 | 0 | 0.0003 | 0.0008 | 0.022 | 0.0006 | 0.01 | 0 | 0.0047 | 0.0024 | 0.0041 | 0.0026 | 0 | 0 | 0.003 | 0.0004 | 0.0001 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0.0005 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0019 | 0.0002 | 0.0019 | 0.001 | 0.0028 | 0.0022 | 0.001 | 0.0002 | 0.001 | 0.0006 | 0.0067 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Endosome | 0.0154 | 0.0002 | 0.0021 | 0.0008 | 0.0191 | 0.0026 | 0.0068 | 0.0001 | 0.0041 | 0.0053 | 0.0149 | 0.0016 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Golgi | 0.0019 | 0 | 0.001 | 0.0018 | 0.0112 | 0.0003 | 0.0053 | 0 | 0.0024 | 0.0021 | 0.0021 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0002 | 0 |
Lipid Particles | 0.0107 | 0.0006 | 0.0027 | 0.0112 | 0.0264 | 0.0011 | 0.026 | 0.0007 | 0.0087 | 0.0217 | 0.0091 | 0.0079 | 0.0011 | 0.0011 | 0.0027 | 0.0144 | 0.0038 | 0.0005 |
Mitochondria | 0.0015 | 0.0006 | 0.0088 | 0.0123 | 0.0148 | 0.0014 | 0.0094 | 0.0006 | 0.0062 | 0.0157 | 0.004 | 0.0032 | 0.0026 | 0.0008 | 0.0073 | 0.0039 | 0.0043 | 0.0017 |
None | 0.0008 | 0.0001 | 0.0001 | 0.0008 | 0.0025 | 0.0002 | 0.0012 | 0.0001 | 0.003 | 0.0004 | 0.0011 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0 |
Nuclear Periphery | 0.865 | 0.8534 | 0.8713 | 0.8647 | 0.7214 | 0.9044 | 0.852 | 0.8787 | 0.8716 | 0.8858 | 0.8654 | 0.9287 | 0.9354 | 0.869 | 0.9158 | 0.8662 | 0.8159 | 0.946 |
Nucleolus | 0.0031 | 0.0072 | 0.004 | 0.0061 | 0.055 | 0.0035 | 0.0022 | 0.0049 | 0.0017 | 0.0039 | 0.0044 | 0.0042 | 0.0014 | 0.0054 | 0.0019 | 0.0128 | 0.0318 | 0.0083 |
Nucleus | 0.0245 | 0.1013 | 0.0501 | 0.0284 | 0.018 | 0.0508 | 0.0086 | 0.0753 | 0.0383 | 0.0225 | 0.027 | 0.0184 | 0.0282 | 0.0872 | 0.0298 | 0.0553 | 0.0998 | 0.0234 |
Peroxisomes | 0.0025 | 0 | 0.0001 | 0.0006 | 0.0057 | 0 | 0.0046 | 0 | 0.0025 | 0.0032 | 0.0017 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0.0058 | 0.0007 | 0.0134 | 0.0263 | 0.0113 | 0.0004 | 0.0008 | 0.0008 | 0.0045 | 0.0013 | 0.0081 | 0.0025 | 0.0002 | 0.0011 | 0.0026 | 0.0246 | 0.0008 | 0.0009 |
Vacuole | 0.0095 | 0.0043 | 0.0019 | 0.001 | 0.0016 | 0.0005 | 0.0017 | 0.0032 | 0.0011 | 0.0015 | 0.0054 | 0.0011 | 0.0004 | 0.0018 | 0.0004 | 0.0003 | 0.0009 | 0.0005 |
Vacuole Periphery | 0.0273 | 0.0306 | 0.0352 | 0.0297 | 0.0354 | 0.0176 | 0.0336 | 0.0348 | 0.0359 | 0.0299 | 0.0405 | 0.0242 | 0.0293 | 0.0324 | 0.0346 | 0.0179 | 0.0412 | 0.0183 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 74.9887 | 81.628 | 55.8341 | 57.4942 | 67.463 | 66.2798 | 78.4995 | 67.9973 | 62.0536 | 66.6903 |
Translational Efficiency | 1.6007 | 1.7498 | 1.8887 | 1.7673 | 1.6727 | 1.5315 | 1.366 | 1.4424 | 1.4686 | 1.5303 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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