Standard name
Human Ortholog
Description Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.11 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.05 0 0 0 0.13 0.18 0.3 0.33 0.39 0.2 0.06 0.08 0.07 0.1 0 0.08 0 0 0 0.08 0.13 0.14
Nucleus 0.1 0.07 0 0.06 0.05 0 0.07 0.07 0.08 0.07 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.73 0.67 0.84 0.84 0.76 0.71 0.62 0.55 0.53 0.42 0.54 0.89 0.79 0.74 0.6 0.6 0.59 0.83 0.79 0.91 0.8 0.73 0.7
Nucleolus 0.23 0.18 0.08 0.13 0.13 0.23 0.31 0.33 0.38 0.4 0.38 0.26 0.24 0.28 0.17 0.21 0.19 0.06 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.27 0.24 0.1 0.13 0.12 0.08 0.08 0.07 0.07 0.07 0.08 0.11 0.11 0.32 0.4 0.32 0.05 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 0 1 0 0 0 1 1 0 0 0 0 0 0 3 3 4
Bud 0 0 0 2 0 1 4 0 2 4 2 0 0 0 0 0 0 0 3 2 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 0 1 2 2 1 6 1 3 4 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 1 8 10 8 4 3 5 4 6 9 27 40 32 21 21 0 2 0 0 0 0
Endoplasmic Reticulum 0 1 3 4 4 0 3 1 0 0 0 0 2 4 6 9 7 0 0 1 2 2 0
Endosome 0 0 3 1 3 10 14 5 2 2 21 1 3 13 3 5 3 0 1 1 4 12 18
Golgi 0 0 0 1 1 0 0 0 0 0 1 0 0 1 2 5 2 0 0 0 2 5 7
Mitochondria 11 14 4 5 13 51 74 113 120 154 83 29 32 38 28 11 17 2 5 11 25 57 58
Nucleus 17 19 15 19 17 18 30 25 29 26 29 12 6 6 4 2 10 6 4 4 0 1 1
Nuclear Periphery 126 194 310 251 255 290 262 206 194 168 220 433 334 393 171 148 124 155 271 380 257 319 290
Nucleolus 39 52 29 38 45 93 131 121 137 159 154 125 102 151 47 51 40 10 21 4 7 5 5
Peroxisomes 0 0 0 0 0 1 0 1 0 0 4 0 3 0 0 0 0 0 0 0 5 12 9
SpindlePole 0 0 0 1 1 3 2 1 2 3 1 2 7 15 1 2 2 1 1 1 0 1 2
Vac/Vac Membrane 33 77 87 30 44 48 32 30 25 27 29 41 47 59 91 99 68 8 28 10 11 9 10
Unique Cell Count 172 288 367 299 334 406 421 372 365 398 406 485 421 532 284 248 210 188 343 419 322 440 415
Labelled Cell Count 227 365 453 361 394 524 558 508 519 548 556 653 567 725 385 353 294 188 343 419 322 440 415


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.7 18.2 15.0 13.6 13.8 11.1 11.0 9.3 8.4 8.1 9.3 15.1 13.9 13.8 21.9 23.5 20.4 13.8 13.4 13.8
Std Deviation (1e-4) 3.8 5.3 3.6 3.7 3.8 2.7 2.8 2.5 2.1 2.1 2.8 3.9 4.3 4.6 7.8 8.0 7.8 3.6 3.1 2.8
Intensity Change (Log2) -0.14 -0.12 -0.43 -0.45 -0.69 -0.84 -0.9 -0.69 0.01 -0.11 -0.12 0.55 0.64 0.44 -0.12 -0.16 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.9 0 0 0 0 0 0 0 4.6 5.1 6.3 5.4 5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 1.8 2.4 0 0 0 3.5 0 0 1.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 6.2 7.7 10.9 11.5 12.8 8.5 3.7 4.4 4.2 5.1 2.6 4.3
Nucleus 1.3 0.6 0.2 1.8 1.6 2.2 1.5 1.8 -1.3 -2.3 -2.9 -2.0 -2.4 0.4
Nuclear Periphery -0.2 -2.7 -4.3 -7.0 -8.6 -9.1 -12.1 -9.1 2.1 -1.9 -3.8 -7.0 -6.9 -6.8
Nucleolus 2.1 2.4 5.7 8.1 8.3 9.6 10.3 9.8 6.7 6.1 7.5 3.4 4.6 4.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole -4.6 -3.6 -4.3 -6.3 -5.8 -6.3 -6.6 -6.4 -6.2 -4.7 -5.0 2.4 4.3 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.9471 13.9696 12.3189 12.0579 10.8741 13.4207 18.0748 17.7718 16.8109 16.4652 16.3817 16.6037 19.3367 16.9161 16.6183 15.2905 16.9491 15.716
Actin 0.0083 0.0001 0.0056 0.0081 0.0436 0.0015 0.0209 0.0002 0.0039 0.0022 0.0024 0.0005 0.0004 0.0001 0.0003 0.0015 0.0003 0.0001
Bud 0.0002 0 0.0003 0.0002 0.0007 0.0002 0.0001 0 0.0002 0.0002 0.0001 0 0 0 0 0.0001 0 0
Bud Neck 0.0003 0 0 0.0002 0.0009 0.0001 0.0007 0 0.0002 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0
Bud Periphery 0.0002 0 0.0006 0.0009 0.0008 0.0002 0.0002 0 0.0003 0.0006 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001
Bud Site 0.0013 0 0.0002 0.0003 0.002 0.0001 0.002 0 0.0012 0.0003 0.0004 0.0001 0 0 0 0.0001 0 0
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0.0002 0 0.0002 0 0.0001 0.0001 0.0002 0.0001 0 0 0 0.0001 0.0001 0
Cytoplasm 0.0018 0.0006 0.0003 0.004 0.0042 0.0123 0.0125 0.0002 0.0085 0.0001 0.0023 0.0033 0 0.0004 0.0001 0.0001 0.0001 0
Cytoplasmic Foci 0.0181 0 0.0003 0.0008 0.022 0.0006 0.01 0 0.0047 0.0024 0.0041 0.0026 0 0 0.003 0.0004 0.0001 0
Eisosomes 0.0001 0 0 0.0005 0.0003 0 0.0003 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0019 0.0002 0.0019 0.001 0.0028 0.0022 0.001 0.0002 0.001 0.0006 0.0067 0.0005 0.0001 0.0002 0.0003 0.0003 0.0001 0.0001
Endosome 0.0154 0.0002 0.0021 0.0008 0.0191 0.0026 0.0068 0.0001 0.0041 0.0053 0.0149 0.0016 0.0001 0.0001 0.0003 0.0002 0.0003 0.0001
Golgi 0.0019 0 0.001 0.0018 0.0112 0.0003 0.0053 0 0.0024 0.0021 0.0021 0.0005 0.0001 0.0001 0.0002 0.0009 0.0002 0
Lipid Particles 0.0107 0.0006 0.0027 0.0112 0.0264 0.0011 0.026 0.0007 0.0087 0.0217 0.0091 0.0079 0.0011 0.0011 0.0027 0.0144 0.0038 0.0005
Mitochondria 0.0015 0.0006 0.0088 0.0123 0.0148 0.0014 0.0094 0.0006 0.0062 0.0157 0.004 0.0032 0.0026 0.0008 0.0073 0.0039 0.0043 0.0017
None 0.0008 0.0001 0.0001 0.0008 0.0025 0.0002 0.0012 0.0001 0.003 0.0004 0.0011 0.0004 0.0002 0.0001 0.0004 0.0003 0.0002 0
Nuclear Periphery 0.865 0.8534 0.8713 0.8647 0.7214 0.9044 0.852 0.8787 0.8716 0.8858 0.8654 0.9287 0.9354 0.869 0.9158 0.8662 0.8159 0.946
Nucleolus 0.0031 0.0072 0.004 0.0061 0.055 0.0035 0.0022 0.0049 0.0017 0.0039 0.0044 0.0042 0.0014 0.0054 0.0019 0.0128 0.0318 0.0083
Nucleus 0.0245 0.1013 0.0501 0.0284 0.018 0.0508 0.0086 0.0753 0.0383 0.0225 0.027 0.0184 0.0282 0.0872 0.0298 0.0553 0.0998 0.0234
Peroxisomes 0.0025 0 0.0001 0.0006 0.0057 0 0.0046 0 0.0025 0.0032 0.0017 0.0002 0.0001 0 0.0001 0.0003 0 0
Punctate Nuclear 0.0058 0.0007 0.0134 0.0263 0.0113 0.0004 0.0008 0.0008 0.0045 0.0013 0.0081 0.0025 0.0002 0.0011 0.0026 0.0246 0.0008 0.0009
Vacuole 0.0095 0.0043 0.0019 0.001 0.0016 0.0005 0.0017 0.0032 0.0011 0.0015 0.0054 0.0011 0.0004 0.0018 0.0004 0.0003 0.0009 0.0005
Vacuole Periphery 0.0273 0.0306 0.0352 0.0297 0.0354 0.0176 0.0336 0.0348 0.0359 0.0299 0.0405 0.0242 0.0293 0.0324 0.0346 0.0179 0.0412 0.0183

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.9887 81.628 55.8341 57.4942 67.463 66.2798 78.4995 67.9973 62.0536 66.6903
Translational Efficiency 1.6007 1.7498 1.8887 1.7673 1.6727 1.5315 1.366 1.4424 1.4686 1.5303

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization
Localization
Cell Percentages nuclear periphery (86%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup2

Nup2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available