Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.96 0.92 0.85 – 0.78 0.72 0.7 0.65 1.0 0.99 0.99 0.97 0.98 0.99 0.95 0.97 0.94 0.87 0.88 0.87
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.14 – 0.18 0.24 0.2 0.21 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 – 14 11 14 32 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 0 0 – 2 3 2 4 0 1 1 0 0 0 0 0 4 8 13 23
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 8 4 18 21 30 – 19 42 34 33 0 4 6 0 1 0 0 0 1 0 0 0
Cytoplasm 76 189 411 347 440 464 566 – 375 464 309 340 364 744 719 112 285 312 76 183 379 402 621 696
Endoplasmic Reticulum 0 1 4 3 16 32 93 – 86 153 89 109 0 12 10 6 17 12 0 1 9 1 4 7
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0
Golgi 0 0 0 0 0 2 5 – 14 7 23 39 0 0 0 2 2 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 2 1 4 8 0 1 0 0 7 1 0 0 0 1 2 0
Nucleus 0 0 0 0 1 0 1 – 1 2 2 1 1 1 0 0 1 0 0 1 0 4 3 3
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Nucleolus 0 0 0 0 0 0 0 – 0 1 3 1 0 0 0 0 0 0 0 0 0 31 34 50
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2
Vac/Vac Membrane 0 0 1 0 0 0 2 – 1 2 1 5 0 0 1 1 2 4 0 0 0 3 10 7
Unique Cell Count 77 191 415 350 459 506 669 480 641 443 523 364 754 726 116 292 314 80 190 403 465 707 802
Labelled Cell Count 78 192 424 354 476 519 701 514 687 481 572 365 763 737 121 315 332 80 190 403 465 707 802


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 138.1 106.7 125.7 118.8 95.6 72.6 63.0 – 54.1 52.7 49.5 49.8 126.9 117.0 112.2 96.3 136.2 132.7 122.2 118.1 124.1
Std Deviation (1e-4) 37.4 26.3 25.6 24.8 20.4 14.5 12.5 – 14.8 12.1 12.0 11.4 28.8 26.3 22.7 39.5 43.2 55.3 41.6 41.5 43.5
Intensity Change (Log2) – – – -0.08 -0.39 -0.79 -1.0 – -1.21 -1.25 -1.34 -1.33 0.01 -0.1 -0.16 -0.38 0.12 0.08 -0.04 -0.09 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 3.5 0 3.7 5.1 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 1.7 1.9 2.2 – 1.8 3.5 3.9 3.3 0 0 -1.6 0 0 0
Cytoplasm 0.2 -2.9 -5.1 -7.7 – -9.5 -11.2 -11.7 -13.0 1.9 -0.5 0 -1.9 -1.5 0.5
Endoplasmic Reticulum 0 2.5 4.2 7.3 – 8.4 10.2 9.0 9.3 0 0.9 0.6 0 3.7 2.6
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 3.5 0 4.7 5.7 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 118.6113 151.7055 135.8903 126.2286 121.4309 126.3665 99.6565 150.8485 132.2474 129.9952 82.1483 125.9547 123.3962 163.4057 151.2112 144.059 134.6873 106.8294
Actin 0.0115 0.0006 0.0128 0.0175 0.0381 0.0008 0.0119 0.0002 0.001 0.0018 0.0156 0.001 0.0015 0.0002 0.0046 0.0008 0.0012 0.0099
Bud 0.0024 0.0009 0.0021 0.0022 0.0009 0.0009 0.0028 0.0009 0.0032 0.003 0.0048 0.0016 0.0015 0.0007 0.0016 0.0018 0.0004 0.0178
Bud Neck 0.0011 0.0007 0.0049 0.0021 0.001 0.0006 0.0011 0.0006 0.0008 0.0013 0.004 0.0008 0.0007 0.0005 0.0008 0.0006 0.0005 0.0019
Bud Periphery 0.0029 0.0006 0.0024 0.0012 0.0012 0.0002 0.0031 0.0003 0.0023 0.0011 0.0033 0.0004 0.0017 0.0004 0.0008 0.0011 0.0001 0.0262
Bud Site 0.0011 0.0007 0.0033 0.0005 0.0007 0.0019 0.0025 0.0006 0.0024 0.0004 0.008 0.0002 0.0034 0.0004 0.0007 0.0006 0.0002 0.0031
Cell Periphery 0.0439 0.0201 0.0195 0.0033 0.0037 0.002 0.0259 0.0055 0.0246 0.005 0.003 0.0004 0.014 0.0099 0.0119 0.0098 0.0003 0.0056
Cytoplasm 0.5486 0.8857 0.71 0.5343 0.6575 0.8167 0.6299 0.9459 0.809 0.7662 0.5186 0.9325 0.693 0.928 0.8328 0.5602 0.9084 0.6426
Cytoplasmic Foci 0.0109 0.0012 0.0043 0.0085 0.0227 0.0131 0.0141 0.0006 0.01 0.0027 0.0619 0.0038 0.0175 0.0007 0.0014 0.0047 0.0192 0.0142
Eisosomes 0.0005 0 0.0007 0.0004 0.0004 0.0007 0.0006 0 0.0001 0.0001 0.0007 0 0.0001 0 0 0.0006 0 0.0001
Endoplasmic Reticulum 0.046 0.009 0.0162 0.0233 0.0244 0.0026 0.0429 0.0054 0.0034 0.0048 0.0023 0.0038 0.0493 0.0081 0.0052 0.0026 0.0031 0.0086
Endosome 0.0126 0.0033 0.0074 0.0092 0.0148 0.0234 0.0097 0.0011 0.0025 0.0089 0.0618 0.0131 0.0068 0.0016 0.004 0.0063 0.004 0.0537
Golgi 0.0025 0.0007 0.0018 0.0025 0.0152 0.0019 0.0032 0.0001 0.0004 0.001 0.0293 0.0017 0.0014 0.0002 0.0028 0.0004 0.0011 0.0068
Lipid Particles 0.0051 0.0008 0.0037 0.0055 0.0104 0.0025 0.0026 0.0002 0.0015 0.0006 0.0219 0.0001 0.0046 0.0006 0.0006 0.0036 0.0066 0.0028
Mitochondria 0.0124 0.0007 0.0067 0.0067 0.0082 0.0088 0.0104 0.0004 0.0066 0.0008 0.085 0.0017 0.0046 0.0004 0.001 0.009 0.0005 0.0208
None 0.004 0.0002 0.0043 0.0028 0.0047 0.0038 0.0226 0.0002 0.0027 0.0005 0.0143 0.0004 0.0033 0.0002 0.0003 0.0037 0.0003 0.0053
Nuclear Periphery 0.0386 0.0041 0.0121 0.0166 0.0274 0.0045 0.0482 0.0029 0.0074 0.0181 0.0059 0.0055 0.0443 0.0034 0.0058 0.021 0.0029 0.0182
Nucleolus 0.0075 0.0002 0.0052 0.009 0.0122 0.081 0.0119 0.0002 0.0024 0.0013 0.008 0.0001 0.0023 0.0002 0.0005 0.0106 0.0003 0.0028
Nucleus 0.011 0.002 0.0051 0.0099 0.0098 0.0103 0.0215 0.0027 0.0063 0.009 0.0085 0.0028 0.0147 0.0025 0.0042 0.0123 0.0025 0.0192
Peroxisomes 0.0017 0 0.0009 0.001 0.0012 0.0005 0.0006 0 0.0008 0.0001 0.0062 0.0001 0.0037 0 0.0001 0.0006 0.006 0.0008
Punctate Nuclear 0.0012 0 0.0006 0.0018 0.0067 0.0008 0.0027 0 0.0002 0.0001 0.0031 0.0001 0.0018 0 0 0.0008 0.0144 0.0406
Vacuole 0.177 0.0605 0.1415 0.2163 0.0918 0.0137 0.1062 0.0275 0.0964 0.136 0.0983 0.0183 0.0974 0.0382 0.0981 0.2559 0.0221 0.07
Vacuole Periphery 0.0576 0.0079 0.0348 0.1256 0.047 0.0092 0.0257 0.0046 0.016 0.0373 0.0355 0.0116 0.0323 0.0038 0.0228 0.0931 0.0061 0.0288

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1208.2853 1256.0884 1492.4585 1355.825 1387.7682 910.3791 986.2858 1036.8601 1082.9226 1194.3315
Translational Efficiency 1.5459 1.5364 1.5071 1.3914 1.7472 2.2811 1.8474 1.7335 1.7579 1.7471

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl26a

Rpl26a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl26a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available