Standard name
Human Ortholog
Description Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.05 0.06 0.06 0.07 0.06 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.68 0.57 0.53 0.35 0.33 0.4 0.42 0.41 0.31 0.32 0.56 0.65 0.54 0.33 0.33 0.44 0.27 0.42 0.41 0.37 0.4 0.4
Endoplasmic Reticulum 0.23 0.14 0.23 0.22 0.2 0.19 0.24 0.27 0.2 0.16 0.25 0.33 0.19 0.18 0 0 0 0.3 0.06 0.23 0.33 0.31 0.26
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.07
Golgi 0 0 0 0.1 0.26 0.31 0.17 0.16 0.16 0.11 0.21 0.07 0.09 0.11 0.39 0.37 0.28 0 0 0 0 0.06 0.08
Mitochondria 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.14 0.1 0.06 0 0
Nucleolus 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.16 0.23 0.19 0.25 0.19 0.18 0.13 0.19 0.3 0.22 0.08 0.1 0.14 0.32 0.35 0.36 0.09 0.18 0.13 0.06 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 1 13 22 20 19 20 21 33 5 3 3 4 1 0 2 3 3 1 1 2
Bud 1 5 2 1 2 3 7 0 8 1 3 7 0 2 1 3 1 3 7 5 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3 0 0 0 2 3 4 3 5 0 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 215 179 144 95 93 112 147 110 134 63 94 144 191 218 83 62 78 118 160 130 67 115 142
Endoplasmic Reticulum 74 38 59 40 53 63 88 71 65 32 74 84 57 74 4 3 3 129 22 74 59 88 93
Endosome 0 0 1 0 2 8 5 0 1 0 2 1 0 10 0 2 2 12 26 12 7 12 24
Golgi 12 1 6 18 71 105 61 42 52 23 63 19 26 46 98 69 51 6 1 1 4 16 29
Mitochondria 0 2 0 0 1 2 2 13 6 13 8 0 1 0 7 1 1 1 1 1 0 2 2
Nucleus 1 1 1 1 0 0 0 1 0 3 1 0 0 0 0 0 0 2 17 4 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 100 51 33 11 10 13
Nucleolus 8 4 3 0 11 17 21 10 11 8 10 5 5 19 16 15 6 1 2 0 1 0 0
Peroxisomes 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
SpindlePole 0 0 2 1 1 2 1 0 0 1 0 0 0 1 4 1 0 7 5 3 3 5 5
Vac/Vac Membrane 37 42 58 33 68 64 64 33 62 62 66 21 28 56 80 66 65 40 68 42 10 22 31
Unique Cell Count 326 264 253 178 268 340 363 263 329 206 295 258 293 401 252 186 179 438 380 322 181 289 358
Labelled Cell Count 349 273 280 190 316 398 418 302 364 232 359 286 311 429 299 223 207 438 380 322 181 289 358


Cytoplasm, Nuclear Periphery, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.8 31.1 32.9 30.9 31.5 31.3 28.8 27.8 26.4 24.2 27.0 34.8 37.5 34.3 47.0 52.1 45.6 34.0 34.8 35.5
Std Deviation (1e-4) 8.5 8.4 8.1 5.8 5.8 6.5 6.9 6.6 6.3 6.7 7.1 7.2 8.1 8.1 15.6 15.6 15.0 6.9 7.1 8.3
Intensity Change (Log2) -0.09 -0.06 -0.07 -0.19 -0.25 -0.32 -0.44 -0.28 0.08 0.19 0.06 0.52 0.66 0.47 0.05 0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 3.5 4.1 3.8 4.4 4.0 5.2 5.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -5.1 -5.8 -4.0 -3.4 -3.9 -5.6 -5.9 -0.3 2.0 -0.6 -5.4 -4.9 -2.7
Endoplasmic Reticulum -0.2 -1.0 -1.4 0.3 1.0 -1.0 -2.1 0.5 2.3 -1.1 -1.5 -7.4 -6.5 -6.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.5 7.8 8.8 5.7 5.3 5.4 3.9 6.7 2.6 3.2 4.2 10.1 9.6 7.9
Mitochondria 0 0 0 0 3.6 0 4.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.1 2.5 2.9 1.9 1.7 0 1.7 0 0 2.5 3.0 3.6 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 0.7 -1.2 -1.6 -3.1 -1.2 1.7 -0.2 -4.6 -4.3 -2.9 2.2 2.9 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.268 40.2542 33.4087 32.715 34.5504 35.6133 30.698 43.6043 38.6632 34.8645 37.6641 38.5315 31.3159 45.3247 39.8152 37.5641 29.9672 40.6356
Actin 0.019 0.0103 0.009 0.0011 0.0016 0.0005 0.0219 0.0005 0.004 0.0137 0.0006 0.0086 0.0159 0.0003 0.0162 0.0039 0.0231 0.0016
Bud 0.0001 0 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0.0006 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0002 0.0001
Bud Neck 0.0007 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004 0.0053 0.0001 0.0003 0.0001 0.0003 0.0005
Bud Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.0003 0 0.0001 0.0009 0.0001 0.0001 0.0002 0 0.0004 0.0002 0.0002 0
Bud Site 0.0014 0.0004 0.0008 0.0001 0.0001 0 0.0015 0 0.0004 0.0008 0 0.0001 0.0027 0 0.0009 0.0002 0.0006 0
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.0567 0.0709 0.0499 0.1523 0.0627 0.0367 0.0397 0.1138 0.0611 0.0945 0.1073 0.0711 0.0449 0.0836 0.0917 0.2052 0.0597 0.1315
Cytoplasmic Foci 0.0156 0.0104 0.0121 0.0485 0.0357 0.0208 0.0136 0.0104 0.013 0.015 0.0342 0.0145 0.0161 0.0063 0.0157 0.0093 0.0551 0.0071
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0 0.0001 0 0 0.0002 0 0.0004 0 0.0006 0
Endoplasmic Reticulum 0.0469 0.0351 0.0293 0.0819 0.054 0.0261 0.0163 0.0205 0.0148 0.0501 0.0093 0.0292 0.0092 0.0142 0.0093 0.0175 0.0121 0.0159
Endosome 0.0239 0.0353 0.0312 0.0672 0.0599 0.0395 0.0202 0.0127 0.0233 0.1043 0.1266 0.0305 0.0146 0.0087 0.02 0.0239 0.0523 0.0184
Golgi 0.0057 0.004 0.0057 0.0086 0.0038 0.0089 0.007 0.0035 0.0061 0.0171 0.0138 0.0089 0.0058 0.0007 0.0033 0.0144 0.0314 0.0012
Lipid Particles 0.0313 0.0431 0.0332 0.0659 0.0171 0.0418 0.0149 0.0265 0.0586 0.0348 0.0219 0.0144 0.028 0.0123 0.0101 0.0275 0.1461 0.0067
Mitochondria 0.005 0.0002 0.0014 0.0011 0.0025 0.0003 0.0037 0.0002 0.0029 0.0377 0.0009 0.0009 0.0055 0.0001 0.0029 0.0253 0.0245 0.0003
None 0.0127 0.0002 0.0013 0.003 0.0005 0.0003 0.0008 0.0003 0.0003 0.0005 0.0003 0.0003 0.0027 0.0002 0.0026 0.0005 0.0052 0.0003
Nuclear Periphery 0.6786 0.7282 0.7574 0.4888 0.6852 0.7514 0.7843 0.7456 0.7431 0.5567 0.6179 0.757 0.7692 0.7749 0.7458 0.6264 0.4826 0.6986
Nucleolus 0.0028 0.0004 0.0009 0.0016 0.0137 0.0006 0.0007 0.0025 0.0076 0.0003 0.0007 0.0004 0.0013 0.0034 0.0007 0.0004 0.0014 0.0002
Nucleus 0.0384 0.0251 0.0282 0.0196 0.024 0.0351 0.0373 0.0373 0.0288 0.0173 0.0282 0.0283 0.0533 0.0735 0.0427 0.02 0.0698 0.0908
Peroxisomes 0.0193 0.0002 0.0021 0.0003 0.0013 0.0002 0.0042 0.0001 0.0004 0.0006 0.0001 0.0004 0.0033 0.0001 0.0023 0.0008 0.0074 0.0001
Punctate Nuclear 0.0118 0.0044 0.0044 0.0128 0.0034 0.005 0.0106 0.011 0.01 0.0023 0.0029 0.0049 0.01 0.0091 0.0174 0.0033 0.011 0.009
Vacuole 0.0093 0.0087 0.0086 0.0264 0.0096 0.0083 0.0065 0.0033 0.0066 0.0163 0.0096 0.0091 0.0029 0.0025 0.0041 0.0044 0.0028 0.0056
Vacuole Periphery 0.0202 0.0228 0.0235 0.0203 0.0246 0.0243 0.0158 0.0115 0.0184 0.0358 0.0251 0.0206 0.0086 0.0098 0.0131 0.0164 0.0133 0.012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.9233 79.3373 75.2878 86.3277 86.8546 66.5258 67.8766 71.3212 70.6537 83.7971
Translational Efficiency 1.2881 1.2735 1.244 1.0377 1.1174 1.3059 1.2378 1.0725 1.0774 1.0035

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
Localization
Cell Percentages cytoplasm (21%), ER (9%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kap95

Kap95


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap95-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available