Standard name
Human Ortholog
Description Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0.05 0.06 0.06 – 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.1 0.08 0.08 0.09 0 0.06 0 0 – 0.05 0.06 0.05 0.1 0.06 0.09 0.59 0.5 0.56 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0.05 0 0 0 0 – 0 0 0 0.14 0.22 0.14 0.1 0.13 0.08 0 0.08 0.09 0.07 0.06 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0.09 0.24 0 0 0 0 0 0 0 0 0.06
Mitochondria 0.89 0.74 0.86 0.88 0.9 0.92 0.95 0.93 – 0.94 0.95 0.95 0.53 0.49 0.43 0.12 0.1 0.07 0.82 0.71 0.74 0.67 0.6 0.59
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 – 0 0 0 0.08 0.09 0.06 0 0.06 0.05 0 0 0.06 0 0.05 0.05
SpindlePole 0 0 0.06 0 0 0 0 0 – 0.07 0.05 0 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0.2 0 0.05 0.07 0.07 0 0.05 – 0.05 0 0 0.25 0.29 0.28 0.05 0.1 0.08 0 0 0 0.07 0.1 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 – 1 0 1 1 0 3 4 5 6 0 4 6 3 6 11
Bud 5 1 23 7 10 25 41 42 – 32 27 31 6 2 4 4 4 9 3 1 3 3 4 4
Bud Neck 0 0 1 0 0 0 1 1 – 1 1 1 0 0 0 4 4 1 0 1 1 3 4 7
Bud Site 2 0 2 0 3 14 10 11 – 13 12 8 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 12 4 3 1 2 0 1 – 1 2 4 4 9 10 17 17 14 0 0 0 0 0 0
Cytoplasm 12 15 25 19 18 30 16 29 – 28 27 27 25 19 28 116 117 126 0 2 2 4 3 3
Endoplasmic Reticulum 0 8 0 0 3 0 1 2 – 0 0 1 2 0 6 13 16 20 0 1 1 3 2 3
Endosome 0 8 12 11 19 4 1 1 – 1 0 0 34 72 47 19 30 18 3 16 29 18 14 12
Golgi 0 8 0 1 0 0 0 0 – 0 0 0 24 31 78 5 5 4 2 7 3 5 8 15
Mitochondria 105 138 284 192 383 496 601 702 – 533 466 534 129 162 140 23 23 15 103 153 253 169 141 170
Nucleus 4 1 1 2 2 22 23 23 – 13 8 9 4 3 1 2 1 1 0 3 0 0 2 1
Nuclear Periphery 0 2 1 1 10 5 8 10 – 6 7 6 1 0 0 0 1 0 0 1 4 2 1 1
Nucleolus 1 1 1 0 10 18 17 17 – 4 12 12 1 1 1 0 0 0 0 0 0 0 2 0
Peroxisomes 0 4 22 7 7 6 8 6 – 7 5 0 19 30 19 3 14 12 5 7 21 9 11 14
SpindlePole 1 4 20 5 14 20 28 32 – 38 24 19 8 12 2 15 12 17 2 1 3 5 4 5
Vac/Vac Membrane 0 38 13 10 28 35 22 35 – 26 13 13 60 95 91 10 24 18 1 9 5 16 22 30
Unique Cell Count 118 187 331 217 425 537 633 753 567 488 560 243 332 328 198 234 224 127 215 345 254 235 287
Labelled Cell Count 130 241 409 258 508 677 777 912 704 604 666 318 437 430 235 273 261 127 215 345 254 235 287


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.3 4.0 4.5 4.4 3.8 3.3 3.3 – 3.1 3.3 3.2 6.5 7.6 7.4 5.4 5.7 5.6 6.4 6.7 6.5
Std Deviation (1e-4) 1.4 0.9 0.7 2.0 1.4 1.0 0.9 0.8 – 0.7 1.2 0.8 1.3 9.7 1.6 1.1 1.3 1.3 1.9 1.9 1.4
Intensity Change (Log2) – – – 0.17 0.15 -0.06 -0.26 -0.27 – -0.37 -0.3 -0.33 0.71 0.92 0.87 0.43 0.5 0.49 0.68 0.73 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 -2.4 -4.3 -3.7 -2.5 -2.9 -1.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 1.4 1.6 4.2 3.7 3.3
Cytoplasm 0.5 -2.0 -1.2 -3.7 -2.6 – -1.6 -1.2 -1.7 1.1 -0.9 0.5 12.8 11.5 12.6
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 4.8 5.5
Endosome 0 0 0 0 0 – 0 0 0 4.5 7.0 4.8 2.8 4.1 2.3
Golgi 0 0 0 0 0 – 0 0 0 5.8 5.7 9.4 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -8.6 -10.2 -11.6 -16.7 -17.9 -18.3
Nucleus 0 0 3.4 3.2 2.8 – 2.3 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0.5 1.1 -0.5 -2.7 -0.3 -0.6
SpindlePole 0 0 0 0 0 – 0 0 0 -1.5 -1.5 -3.9 0.7 -0.5 0.7
Vacuole 0.4 1.6 1.6 -0.4 0.5 – 0.5 -1.0 -1.4 7.4 8.6 8.4 0.6 3.0 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4094 7.4656 6.9784 6.3769 7.1467 7.3827 9.3322 10.1533 9.9125 8.7227 9.1655 10.251 7.4227 7.4299 7.6427 6.638 6.802 7.5005
Actin 0.0111 0.0186 0.0054 0.0015 0.0028 0.0035 0.0111 0.0039 0.0053 0.009 0.004 0.0036 0.0052 0.0192 0.0047 0.002 0.0031 0.0018
Bud 0.0017 0.0034 0.0012 0.0025 0.0037 0.0002 0.0004 0.0003 0.001 0.0004 0.0009 0.0003 0.0004 0.0009 0.0003 0.0005 0.0021 0.0001
Bud Neck 0.0014 0.001 0.0005 0.0002 0.0003 0.0014 0.0005 0.0003 0.0001 0.0001 0.0003 0.0002 0.0003 0.0007 0.0002 0.0003 0.0001 0.0001
Bud Periphery 0.0028 0.0063 0.0024 0.0057 0.0064 0.0005 0.001 0.0006 0.0025 0.0007 0.001 0.0018 0.001 0.0012 0.0009 0.0012 0.0089 0.0002
Bud Site 0.0074 0.0111 0.0034 0.0022 0.0011 0.0003 0.0021 0.0028 0.0015 0.0005 0.0014 0.0003 0.0007 0.0066 0.0006 0.0013 0.0009 0.0001
Cell Periphery 0.0007 0.0016 0.0012 0.0009 0.0005 0.0002 0.0006 0.0017 0.0004 0.0002 0.0002 0.0003 0.0005 0.0006 0.0004 0.0003 0.0005 0.0002
Cytoplasm 0.0005 0.0021 0.0005 0.0002 0.0006 0.0002 0.0015 0.0003 0.0004 0.0001 0.0006 0.0001 0.0003 0.0005 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0116 0.0127 0.0074 0.0023 0.0042 0.0038 0.0091 0.0107 0.005 0.0016 0.0086 0.0029 0.0066 0.0075 0.0039 0.0028 0.002 0.0019
Eisosomes 0.0004 0.0003 0.0003 0.0002 0.0001 0.0001 0.0005 0.0018 0.0003 0.0002 0.0001 0.0002 0.0002 0.0003 0.0003 0.0001 0.0017 0.0013
Endoplasmic Reticulum 0.0005 0.0012 0.0004 0.0006 0.0004 0.0003 0.0009 0.0002 0.0002 0.0001 0.0002 0.0003 0.0029 0.0022 0.0002 0.0001 0.0001 0.0002
Endosome 0.0614 0.0539 0.0247 0.0176 0.038 0.019 0.0212 0.0291 0.0111 0.0105 0.0305 0.0112 0.0266 0.0336 0.011 0.0187 0.0156 0.0143
Golgi 0.0569 0.0724 0.0334 0.0307 0.043 0.0387 0.0363 0.0578 0.014 0.0097 0.0351 0.0296 0.0413 0.0644 0.0331 0.0281 0.0202 0.0416
Lipid Particles 0.0225 0.0116 0.0046 0.0052 0.0015 0.0059 0.0172 0.0071 0.0012 0.0037 0.003 0.0047 0.0218 0.0096 0.005 0.0032 0.0012 0.002
Mitochondria 0.7872 0.7422 0.8879 0.9072 0.8516 0.907 0.8732 0.8697 0.942 0.9517 0.9024 0.9339 0.8581 0.8197 0.9243 0.935 0.931 0.9275
None 0.0003 0.0004 0.0001 0.0002 0.0001 0 0.0008 0.0001 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Nuclear Periphery 0.0008 0.0005 0.0001 0.0026 0.0004 0.0003 0.0009 0.0002 0.0002 0 0.0001 0.0007 0.0009 0.0037 0.0002 0 0.0001 0.0002
Nucleolus 0.0022 0.0085 0.0009 0.0007 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.004 0.0047 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0007 0.0015 0.0004 0.0005 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0012 0.0001 0.0001 0.0002 0.0001
Peroxisomes 0.0054 0.0046 0.0062 0.0038 0.0041 0.0033 0.0131 0.0072 0.0089 0.0031 0.0051 0.0037 0.0082 0.005 0.0042 0.0033 0.0048 0.002
Punctate Nuclear 0.0003 0.0002 0.0001 0.0001 0 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0 0
Vacuole 0.0105 0.0278 0.0114 0.0096 0.0227 0.0028 0.0038 0.0022 0.0026 0.0019 0.0036 0.0016 0.0087 0.0079 0.0044 0.0011 0.0038 0.0017
Vacuole Periphery 0.0137 0.0182 0.0074 0.0055 0.0182 0.0122 0.0048 0.0037 0.0025 0.0061 0.0024 0.0044 0.0117 0.0103 0.0059 0.0016 0.0035 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.2881 34.0888 30.1912 26.4129 39.8721 36.8827 60.5955 52.5294 48.5724 43.2284
Translational Efficiency 1.2283 1.3261 0.7782 1.0299 0.8768 1.2118 0.9525 0.8697 0.7616 0.8319

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Dic1

Dic1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dic1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available