Standard name | |
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Human Ortholog | |
Description | Part of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 2 | 1 | 0 | – | – | 0 | 1 | 1 | 3 | 0 | 1 | 3 |
Bud | 7 | 5 | 6 | 16 | 19 | 32 | 32 | 55 | – | 49 | 54 | 43 | 1 | 1 | 0 | – | – | 2 | 0 | 1 | 2 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | – | 0 | 0 | 0 | 2 | 1 | 2 | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Site | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | – | 9 | 7 | 7 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 10 | 20 | 32 | 34 | 56 | 55 | 66 | – | 53 | 56 | 36 | 14 | 14 | 14 | – | – | 20 | 2 | 1 | 3 | 0 | 3 | 1 |
Endoplasmic Reticulum | 1 | 8 | 2 | 4 | 1 | 1 | 0 | 2 | – | 1 | 0 | 0 | 2 | 5 | 2 | – | – | 12 | 0 | 0 | 1 | 1 | 3 | 2 |
Endosome | 99 | 42 | 249 | 238 | 368 | 215 | 265 | 198 | – | 29 | 22 | 36 | 182 | 195 | 159 | – | – | 151 | 172 | 184 | 412 | 234 | 502 | 465 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 2 | 2 | 1 | 1 | 7 | 11 |
Mitochondria | 61 | 127 | 145 | 157 | 144 | 283 | 354 | 411 | – | 424 | 307 | 317 | 1 | 1 | 3 | – | – | 28 | 8 | 11 | 14 | 7 | 14 | 17 |
Nucleus | 0 | 0 | 0 | 1 | 4 | 4 | 2 | 5 | – | 2 | 4 | 0 | 2 | 2 | 6 | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 2 | 1 | – | – | 2 | 1 | 2 | 3 | 1 | 2 | 3 |
Nucleolus | 13 | 10 | 30 | 16 | 13 | 16 | 19 | 39 | – | 12 | 7 | 4 | 10 | 10 | 2 | – | – | 11 | 1 | 2 | 1 | 1 | 1 | 1 |
Peroxisomes | 6 | 1 | 26 | 71 | 118 | 100 | 169 | 128 | – | 54 | 36 | 47 | 9 | 11 | 3 | – | – | 2 | 6 | 2 | 14 | 4 | 10 | 11 |
SpindlePole | 50 | 18 | 135 | 137 | 146 | 151 | 229 | 245 | – | 115 | 111 | 116 | 123 | 119 | 76 | – | – | 74 | 7 | 13 | 28 | 16 | 20 | 14 |
Vac/Vac Membrane | 25 | 24 | 34 | 18 | 37 | 24 | 30 | 17 | – | 7 | 3 | 11 | 43 | 43 | 88 | – | – | 45 | 4 | 13 | 12 | 6 | 12 | 14 |
Unique Cell Count | 170 | 196 | 444 | 465 | 550 | 589 | 774 | 848 | 570 | 432 | 453 | 297 | 301 | 269 | 229 | 212 | 241 | 503 | 282 | 588 | 556 | |||
Labelled Cell Count | 272 | 253 | 651 | 696 | 885 | 882 | 1158 | 1170 | 756 | 608 | 619 | 394 | 406 | 356 | 349 | 212 | 241 | 503 | 282 | 588 | 556 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.8 | 4.2 | 4.3 | 4.3 | 3.9 | 3.6 | 3.9 | – | 3.3 | 3.4 | 3.4 | 6.3 | 6.3 | 6.3 | 3.9 | 4.7 | 5.6 | 6.4 | 6.3 | 6.7 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.8 | 1.2 | 1.5 | 1.4 | 0.8 | 1.0 | – | 0.9 | 1.1 | 0.8 | 1.1 | 1.1 | 0.8 | 0.9 | 1.0 | 1.2 | 1.9 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.02 | -0.14 | -0.25 | -0.14 | – | -0.36 | -0.31 | -0.31 | 0.56 | 0.56 | 0.58 | -0.14 | 0.14 | 0.41 | 0.58 | 0.57 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1929 | 1.037 | 1.2206 | 0.8191 | 0.8441 | 0.9814 | 0.3274 | 1.4156 | 0.999 | 0.5532 | 0.6132 | 1.0833 | -0.0031 | 1.1326 | 0.4838 | 0.6534 | 0.3336 | 0.3808 |
Actin | 0.0395 | 0.0006 | 0.0297 | 0.0001 | 0.0013 | 0.0032 | 0.0099 | 0.0281 | 0.0158 | 0.0007 | 0.0499 | 0.0022 | 0.0178 | 0 | 0.0001 | 0.002 | 0.0008 | 0.0001 |
Bud | 0.0006 | 0.0014 | 0.0002 | 0 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0055 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0009 | 0.0069 | 0.0023 | 0.0017 | 0.0002 | 0.0005 | 0.0007 | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Bud Periphery | 0.0009 | 0.0009 | 0.0002 | 0 | 0.002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0088 | 0.0064 | 0.0012 | 0.0002 | 0.0018 | 0.0005 | 0.0008 | 0.0007 | 0.0026 | 0.0006 | 0.0012 | 0.0005 | 0.001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0007 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.021 | 0.0458 | 0.0056 | 0.0018 | 0.0433 | 0.0112 | 0.0059 | 0.0037 | 0.0065 | 0.0017 | 0.0077 | 0.0022 | 0.0069 | 0.0108 | 0.0035 | 0.0058 | 0.0011 | 0.0024 |
Cytoplasmic Foci | 0.1993 | 0.1917 | 0.1816 | 0.1317 | 0.142 | 0.1906 | 0.2193 | 0.1898 | 0.2005 | 0.1734 | 0.1756 | 0.1756 | 0.1634 | 0.1949 | 0.1934 | 0.1669 | 0.1748 | 0.1706 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0.0007 | 0 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0101 | 0.0249 | 0.0002 | 0.0096 | 0.0012 | 0.0013 | 0.0004 | 0.0009 | 0.0001 | 0.0008 | 0.0005 | 0.0015 | 0.0011 | 0.0007 | 0.0004 | 0.0003 | 0.0002 |
Endosome | 0.3105 | 0.3694 | 0.4143 | 0.6475 | 0.5727 | 0.3971 | 0.3206 | 0.4177 | 0.3532 | 0.4227 | 0.3237 | 0.2797 | 0.3514 | 0.4234 | 0.3827 | 0.4979 | 0.5447 | 0.3462 |
Golgi | 0.0273 | 0.0223 | 0.0635 | 0.0535 | 0.0342 | 0.0531 | 0.046 | 0.0523 | 0.0651 | 0.0657 | 0.0469 | 0.039 | 0.0382 | 0.0375 | 0.0487 | 0.0566 | 0.0749 | 0.0514 |
Lipid Particles | 0.2107 | 0.2027 | 0.1848 | 0.1274 | 0.0713 | 0.2027 | 0.2426 | 0.1911 | 0.1873 | 0.1665 | 0.2295 | 0.3686 | 0.2739 | 0.2005 | 0.2593 | 0.1492 | 0.1046 | 0.355 |
Mitochondria | 0.0096 | 0.005 | 0.0138 | 0.0024 | 0.0036 | 0.0039 | 0.0053 | 0.0046 | 0.0183 | 0.0082 | 0.0012 | 0.0031 | 0.0042 | 0.001 | 0.009 | 0.0041 | 0.0086 | 0.0013 |
None | 0.0321 | 0.0513 | 0.0005 | 0.0002 | 0.007 | 0.0072 | 0.0128 | 0.0009 | 0.0306 | 0.0003 | 0.0417 | 0.0158 | 0.0379 | 0.0382 | 0.0086 | 0.0132 | 0.0001 | 0.0009 |
Nuclear Periphery | 0.0162 | 0.0012 | 0.0006 | 0.0001 | 0.0427 | 0.007 | 0.0013 | 0.0004 | 0.0009 | 0.0001 | 0.0003 | 0.0011 | 0.0033 | 0.0009 | 0.0009 | 0.0018 | 0.0002 | 0.0003 |
Nucleolus | 0.0091 | 0.0016 | 0.0003 | 0.0005 | 0.0006 | 0.001 | 0.0007 | 0.0005 | 0.0013 | 0.0004 | 0.0002 | 0.0003 | 0.0011 | 0.0005 | 0.0011 | 0.0032 | 0.0002 | 0.0002 |
Nucleus | 0.0153 | 0.001 | 0.0002 | 0.0001 | 0.0238 | 0.0016 | 0.0006 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0006 | 0.0002 | 0.0017 | 0 | 0.0001 |
Peroxisomes | 0.0481 | 0.0523 | 0.0566 | 0.024 | 0.0208 | 0.0945 | 0.1158 | 0.0924 | 0.0994 | 0.1365 | 0.1152 | 0.1046 | 0.0769 | 0.0493 | 0.0709 | 0.0727 | 0.0825 | 0.0626 |
Punctate Nuclear | 0.0142 | 0.0068 | 0.0039 | 0.0017 | 0.0031 | 0.0105 | 0.0045 | 0.0045 | 0.0038 | 0.0005 | 0.0017 | 0.0015 | 0.0094 | 0.0015 | 0.0075 | 0.007 | 0.0005 | 0.0038 |
Vacuole | 0.0147 | 0.0252 | 0.0153 | 0.0062 | 0.0137 | 0.0095 | 0.004 | 0.0077 | 0.0071 | 0.0201 | 0.0021 | 0.0031 | 0.0076 | 0.0358 | 0.0097 | 0.0124 | 0.0039 | 0.0033 |
Vacuole Periphery | 0.0032 | 0.0035 | 0.0023 | 0.0021 | 0.005 | 0.0038 | 0.0012 | 0.0022 | 0.0026 | 0.0021 | 0.0009 | 0.0013 | 0.0018 | 0.0034 | 0.0027 | 0.0034 | 0.0024 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7771 | 11.2088 | 8.3174 | 11.7155 | 7.5866 | 3.3418 | 8.8777 | 14.0235 | 11.1798 | 12.2658 |
Translational Efficiency | 0.4366 | 0.4647 | 0.6261 | 0.4527 | 0.6006 | 1.1782 | 0.8398 | 0.5584 | 0.4668 | 0.5236 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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