Standard name
Human Ortholog
Description Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0.05 – 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.95 0.98 0.97 0.88 – 0.75 0.67 0.64 0.65 0.62 0.97 0.99 0.98 1.0 0.75 – 0.93 0.86 0.9 0.81 0.86 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0
Mitochondria 0 0 0.1 0.18 0.05 0.29 – 0.39 0.56 0.54 0.63 0.64 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0.15 – 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 – 2 1 0 0 0 2
Bud 1 2 7 2 0 0 – 8 7 5 9 11 0 0 0 0 0 – 0 1 1 1 6 10
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 – 0 0 1 1 5 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 1 1 1 1 – 5 2 0 4 3 1 0 1 2 1 – 0 0 0 0 0 0
Cytoplasm 180 188 187 235 260 243 – 299 253 243 171 204 209 222 325 16 15 – 177 191 173 99 225 233
Endoplasmic Reticulum 1 0 0 0 0 0 – 0 1 1 0 0 2 3 1 1 1 – 0 1 2 0 1 0
Endosome 0 0 0 1 7 4 – 4 0 2 0 1 0 0 1 0 1 – 1 1 0 5 3 5
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 – 1 0 0 5 6 4
Mitochondria 3 8 20 43 13 81 – 155 212 207 167 210 3 0 1 0 0 – 0 6 6 0 3 3
Nucleus 0 0 0 0 0 0 – 3 2 6 1 1 1 0 1 1 1 – 0 0 0 0 0 2
Nuclear Periphery 1 1 0 0 0 0 – 1 2 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 1 1 2 0 0 1 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 – 2 0 1 0 1 0 0 1 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 4 0 3 5 3 0 0 0 0 0 – 0 0 1 2 1 3
Vac/Vac Membrane 1 1 1 0 1 7 – 19 12 5 4 5 0 0 0 0 3 – 1 13 0 4 5 6
Unique Cell Count 184 195 196 241 269 277 397 377 382 263 329 216 225 331 16 20 190 222 192 124 263 284
Labelled Cell Count 187 201 216 282 282 337 500 492 475 363 446 216 226 334 20 22 190 222 192 124 263 284


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.7 4.0 3.9 4.7 3.5 – 4.0 3.1 3.6 3.3 3.3 5.3 5.2 5.1 6.6 7.5 – 5.7 5.4 5.3
Std Deviation (1e-4) 1.4 0.9 1.8 1.7 2.0 1.2 – 1.9 1.1 0.9 1.0 1.0 1.7 1.4 1.6 1.4 1.8 – 2.8 2.1 2.0
Intensity Change (Log2) – – – -0.01 0.24 -0.19 – -0.0 -0.37 -0.15 -0.29 -0.28 0.42 0.38 0.35 0.74 0.91 – 0.51 0.45 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4136 0.1836 -0.1488 -0.4315 -0.5659 -0.028 -0.2728 0.0998 0.0943 -0.1137 -0.0706 -0.174 0.9827 1.2203 0.9674 0.8719 0.8572 0.8276
Actin 0.0016 0.0009 0.0007 0.0042 0 0.0033 0.0158 0.0015 0.0076 0.0004 0.0107 0.0012 0.0414 0.0004 0.0169 0.0181 0.0086 0.0007
Bud 0.0003 0.001 0.0018 0.0003 0 0.0001 0.0024 0.005 0.0063 0.0011 0.0017 0.0002 0.0014 0.0121 0.0008 0.0002 0.0006 0.0013
Bud Neck 0.0019 0.0003 0.0002 0.0002 0 0.0013 0.0027 0.0003 0.0005 0.0009 0.0053 0.0004 0.0071 0.0001 0.0037 0.001 0.0004 0.0012
Bud Periphery 0.0005 0.0011 0.0024 0.0005 0 0.0001 0.0073 0.0073 0.0094 0.002 0.0032 0.0002 0.0047 0.0036 0.0013 0.0002 0.0009 0.0013
Bud Site 0.003 0.0044 0.0006 0.0001 0 0.0004 0.0084 0.0075 0.0064 0.0046 0.0148 0.0003 0.0041 0.0021 0.0083 0.0019 0.0016 0.0003
Cell Periphery 0.0004 0.0002 0.0001 0 0 0 0.0013 0.0003 0.0005 0.0004 0.0007 0 0.0008 0.0001 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.1252 0.1853 0.2133 0.0983 0.176 0.2742 0.2005 0.2843 0.2447 0.3385 0.1982 0.348 0.2407 0.3333 0.2876 0.2993 0.3542 0.4865
Cytoplasmic Foci 0.0033 0.0055 0.0054 0.0047 0.0016 0.0072 0.0222 0.0147 0.0195 0.0153 0.0319 0.0131 0.0206 0.0032 0.0066 0.0071 0.0158 0.0074
Eisosomes 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0007 0.0003 0.0002 0.0001 0.0002 0 0.0003 0 0.0004 0.0001 0.0002 0
Endoplasmic Reticulum 0.0012 0.0018 0.0011 0.0003 0.0002 0.0009 0.0052 0.0021 0.0039 0.0008 0.0012 0.0012 0.006 0.0006 0.002 0.0034 0.0011 0.0014
Endosome 0.001 0.0014 0.001 0.0004 0.0002 0.0011 0.0175 0.0099 0.0239 0.0266 0.0561 0.006 0.0101 0.0004 0.0095 0.0084 0.0077 0.0022
Golgi 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0047 0.0019 0.0153 0.0041 0.0354 0.0019 0.0029 0.0001 0.0027 0.0011 0.0054 0.0004
Lipid Particles 0.0005 0.0003 0.0001 0.0002 0 0.0002 0.0086 0.0006 0.0056 0.0015 0.0068 0.0016 0.0103 0.0001 0.0051 0.0005 0.0294 0.0001
Mitochondria 0.0008 0.0003 0.0004 0.0002 0 0.0001 0.0123 0.0008 0.0123 0.0014 0.0303 0.007 0.006 0.0005 0.0138 0.0006 0.0492 0.0097
None 0.8522 0.7924 0.7692 0.8882 0.821 0.7081 0.6546 0.6515 0.6295 0.5781 0.5712 0.6092 0.609 0.6402 0.6247 0.6385 0.4629 0.4807
Nuclear Periphery 0.0048 0.0005 0.0005 0.0003 0.0001 0.0004 0.0052 0.0012 0.002 0.0008 0.0013 0.0011 0.0109 0.0004 0.0017 0.0063 0.0006 0.0007
Nucleolus 0.0002 0.0003 0.0002 0.0001 0 0.0001 0.0008 0.0003 0.0003 0.0007 0.0003 0.0003 0.0006 0.0001 0.0002 0.0003 0.0002 0.0001
Nucleus 0.0014 0.0014 0.0012 0.0005 0.0003 0.001 0.0026 0.0026 0.0021 0.0024 0.0014 0.0027 0.0049 0.0014 0.001 0.0037 0.0023 0.0026
Peroxisomes 0.0002 0.0002 0.0001 0.0004 0 0.0001 0.0168 0.0037 0.0043 0.001 0.0109 0.0027 0.0095 0.0002 0.0099 0.0004 0.0561 0.0013
Punctate Nuclear 0.0006 0.0012 0.0008 0.0006 0.0001 0.0009 0.0026 0.0013 0.0014 0.0009 0.0027 0.0017 0.0055 0.0008 0.0012 0.0043 0.0007 0.001
Vacuole 0.0004 0.0007 0.0004 0.0002 0.0001 0.0003 0.0057 0.0027 0.0035 0.0163 0.0074 0.0009 0.0022 0.0004 0.0017 0.004 0.0015 0.0007
Vacuole Periphery 0.0004 0.0001 0.0001 0.0001 0 0 0.002 0.0003 0.0008 0.0023 0.0083 0.0002 0.001 0.0001 0.0006 0.0005 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0464 8.9671 6.6539 15.0199 8.6704 6.6836 9.8221 11.2188 11.7122 12.2658
Translational Efficiency 1.6675 1.8892 4.1976 2.3201 2.3776 4.1748 2.652 2.4485 2.5257 1.6572

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Grx8

Grx8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Grx8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available