Standard name
Human Ortholog
Description Mitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.09 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0 0.05 0.07 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Golgi 0 0.16 0.2 0.18 0.22 0 0 0 0 0 0 0 0.41 0.37 0.49 0.4 0.23 0.2 0 0.07 0 0.07 0.08 0.1
Mitochondria 0.93 0.82 0.77 0.9 0.75 0.83 0.82 0.74 0.88 0.86 0.94 0.89 0.75 0.74 0.58 0.08 0.09 0.06 0.84 0.78 0.82 0.72 0.7 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.07 0 0 0.09 0.11 0.09 0.06 0.07 0.08 0.06 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0.05 0.08 0.06 0.07 0.06 0.05 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.15 0.14 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.22 0.14 0.09 0.15 0.12 0.07 0.11 0.1 0.1 0 0.1 0.19 0.16 0.2 0.32 0.29 0.35 0 0 0 0.09 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 8 8 0 1 2 0 2 2 1 7 14 10 0 1 6 5 6 1 4 12 21
Bud 6 2 1 3 3 4 10 14 9 10 10 10 1 2 1 3 4 9 0 2 0 1 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Bud Site 0 1 0 0 0 2 1 5 1 4 2 2 0 0 0 0 0 2
Cell Periphery 6 20 1 8 15 2 8 7 6 14 6 9 17 27 38 20 16 20 1 0 0 0 0 4
Cytoplasm 4 0 2 5 11 7 10 10 3 8 7 4 6 27 21 12 17 6 1 1 0 1 3 2
Endoplasmic Reticulum 8 7 0 0 3 1 1 7 2 5 2 4 7 6 11 6 6 2 0 0 0 0 3 8
Endosome 4 8 0 6 39 16 27 41 4 12 6 10 8 12 17 15 11 17 10 14 1 6 23 32
Golgi 3 49 9 44 85 4 9 7 7 12 5 7 114 182 293 90 58 43 8 24 1 14 47 72
Mitochondria 286 254 34 217 293 306 462 426 348 480 482 504 209 364 343 18 23 13 267 275 66 159 411 529
Nucleus 1 1 0 0 1 3 6 4 3 4 4 2 0 2 1 5 8 4 0 0 0 2 3 0
Nuclear Periphery 20 9 3 2 13 32 64 53 25 41 43 36 3 3 6 9 20 8 2 4 0 2 5 6
Nucleolus 9 3 2 4 7 18 47 35 27 35 25 19 1 3 2 10 17 13 0 0 0 0 1 1
Peroxisomes 1 6 1 6 11 9 12 18 1 6 0 4 2 9 8 13 39 31 10 6 2 4 18 17
SpindlePole 2 1 1 2 5 3 9 19 7 7 5 4 2 3 1 7 8 9 0 1 0 0 1 3
Vac/Vac Membrane 42 68 6 21 58 46 40 63 40 57 19 55 52 81 118 73 74 75 6 12 2 20 47 48
Unique Cell Count 308 311 44 240 392 370 566 577 395 555 511 565 278 493 596 225 253 215 317 353 81 221 590 759
Labelled Cell Count 394 431 60 326 552 453 707 711 483 697 618 671 429 735 870 281 303 258 317 353 81 221 590 759


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 9.0 8.2 8.1 8.9 8.1 8.0 8.0 7.1 7.5 6.9 7.3 9.4 9.1 9.6 11.0 11.3 10.3 8.3 8.4 8.9
Std Deviation (1e-4) 1.4 1.4 1.8 1.1 1.5 1.9 1.8 1.6 1.5 1.5 1.7 1.7 1.7 1.4 1.5 2.8 3.1 3.0 1.5 1.6 1.7
Intensity Change (Log2) -0.03 0.11 -0.02 -0.04 -0.03 -0.21 -0.14 -0.24 -0.18 0.2 0.15 0.23 0.43 0.46 0.33 0.02 0.03 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.3 0.2 0 0 0 0 0 0 0 2.6 2.2 3.7 2.5 0.4 -0.1
Mitochondria 2.5 -0.4 0.9 0.7 -0.5 2.0 1.7 4.2 2.4 -0.3 -0.5 -2.6 -10.6 -10.6 -11.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4 2.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.2 -0.2 0 0 0 0 0 0 0.8 0.5 1.0 2.5 2.2 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6079 4.8366 4.376 4.291 4.1769 4.527 3.2419 5.102 4.4326 3.7637 3.3388 4.4476 2.8172 3.728 3.2127 3.0813 1.8676 3.3873
Actin 0.0188 0.0024 0.017 0.019 0.0009 0.0009 0.0441 0.0085 0.0065 0.0007 0.0187 0.006 0.0123 0.011 0.0085 0.0288 0.0035 0.0004
Bud 0.0003 0.0003 0.0006 0.0003 0.0001 0.0001 0.004 0.0024 0.0011 0.0001 0.001 0.0002 0.0002 0.0005 0.0003 0.0002 0.0005 0
Bud Neck 0.0005 0.0001 0.001 0.0021 0.0001 0.0001 0.0008 0.0005 0.0009 0.0001 0.0006 0.0002 0.0003 0.0002 0.0001 0.0002 0.0003 0
Bud Periphery 0.0006 0.0004 0.0009 0.0007 0.0003 0.0002 0.0089 0.0035 0.0016 0.0002 0.0013 0.0002 0.0005 0.0007 0.0008 0.0003 0.0012 0.0001
Bud Site 0.0014 0.0015 0.007 0.0011 0.0001 0.0002 0.0041 0.0049 0.0058 0.0002 0.003 0.0007 0.0008 0.0027 0.0004 0.0005 0.0012 0
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0005 0.0015 0.0006 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0006 0
Cytoplasm 0.0041 0.0002 0.0011 0.0002 0.0001 0.0001 0.0063 0.0004 0.0023 0 0.0009 0.0006 0.0047 0.0002 0.0001 0.0001 0.0009 0
Cytoplasmic Foci 0.0148 0.0042 0.0112 0.0046 0.0034 0.0024 0.0102 0.0113 0.0116 0.0065 0.0448 0.0209 0.0095 0.0044 0.0019 0.0022 0.0085 0.0012
Eisosomes 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0006 0.0004 0.0001 0.0002 0.0002 0.0003 0.0002 0.0005 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0022 0.0001 0.0004 0.0001 0.0004 0.0001 0.0013 0.0002 0.0009 0 0.0039 0.0003 0.0016 0.0001 0.0002 0.0001 0.0015 0.0001
Endosome 0.0393 0.0203 0.0212 0.0047 0.03 0.009 0.0345 0.0251 0.0142 0.0166 0.0389 0.0064 0.0233 0.0156 0.0081 0.0071 0.0222 0.0136
Golgi 0.0381 0.0269 0.0228 0.0065 0.0363 0.0238 0.0216 0.021 0.0158 0.0071 0.0697 0.0132 0.026 0.0203 0.0121 0.0179 0.0246 0.0215
Lipid Particles 0.0366 0.0024 0.0054 0.0035 0.0206 0.003 0.0586 0.0127 0.0125 0.0302 0.0248 0.0178 0.0414 0.0226 0.016 0.0013 0.0403 0.0024
Mitochondria 0.7731 0.9172 0.8789 0.9486 0.8578 0.951 0.7351 0.848 0.877 0.9209 0.6795 0.9149 0.8238 0.8832 0.9274 0.9353 0.8368 0.9461
None 0.0027 0.0001 0.0005 0.0002 0.0001 0 0.0027 0.0002 0.0031 0.0001 0.0004 0.0011 0.0054 0 0.0001 0 0.0024 0
Nuclear Periphery 0.0038 0.0001 0.0009 0.0001 0.0005 0.0002 0.0065 0.0002 0.0015 0 0.0542 0.0002 0.0124 0.0003 0.0029 0 0.0024 0.0001
Nucleolus 0.0062 0.0001 0.0002 0.0001 0.0002 0.0001 0.0012 0.0018 0.0012 0.0019 0.0002 0.0021 0.0009 0.0003 0.0002 0 0.0122 0
Nucleus 0.0024 0.0001 0.0004 0.0001 0.0002 0.0001 0.0061 0.0005 0.002 0.0002 0.0055 0.0005 0.0028 0.0002 0.0006 0 0.0091 0
Peroxisomes 0.0234 0.0081 0.0176 0.0067 0.0365 0.0036 0.0373 0.0298 0.0262 0.0106 0.0412 0.0108 0.0228 0.0261 0.006 0.0027 0.0114 0.0067
Punctate Nuclear 0.01 0 0.0009 0.0003 0.0001 0 0.0009 0.0001 0.0018 0.0001 0.0015 0.0015 0.0029 0 0 0 0.0069 0
Vacuole 0.0081 0.0083 0.0041 0.0003 0.0034 0.0014 0.0074 0.0151 0.0081 0.002 0.003 0.0008 0.0028 0.0058 0.0044 0.0006 0.0064 0.0032
Vacuole Periphery 0.0129 0.0069 0.0075 0.0006 0.0087 0.0038 0.0074 0.0116 0.0047 0.0022 0.0064 0.0013 0.005 0.0053 0.0088 0.0022 0.0068 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4153 6.9956 7.7865 16.6474 13.7698 7.2626 10.8624 17.0498 17.0877 16.6121
Translational Efficiency 1.5077 2.2169 2.3669 1.1923 1.0705 2.8712 1.893 1.0988 1.3837 1.4037

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ssq1

Ssq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available