Standard name | |
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Human Ortholog | |
Description | Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 18 | 15 | 4 | 11 | 17 | 9 |
Bud | 0 | 4 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 5 | 8 | 0 | 4 | 7 | 10 |
Bud Neck | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 15 | 9 | 2 | 5 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 4 | 0 | 1 | 3 | 2 | 2 | 2 | 3 | 3 | 1 | 1 | 1 | 8 | 14 | 13 | 6 | 7 | 7 | 1 | 1 | 0 | 3 |
Cytoplasm | 380 | 450 | 70 | 88 | 66 | 87 | 84 | 151 | 78 | 37 | 52 | 29 | 148 | 168 | 178 | 396 | 205 | 263 | 310 | 360 | 69 | 47 | 102 | 147 |
Endoplasmic Reticulum | 4 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 35 | 12 | 23 | 5 | 1 | 0 | 0 | 2 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 4 | 3 | 5 | 0 | 1 | 2 | 2 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 2 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 1 | 15 | 8 | 4 | 6 | 11 | 19 | 39 | 34 | 21 | 20 | 26 | 1 | 0 | 3 | 18 | 2 | 3 | 13 | 22 | 19 | 4 | 7 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 4 | 7 | 0 | 0 | 5 | 7 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 4 | 4 | 9 | 0 | 1 | 1 | 4 |
Unique Cell Count | 393 | 468 | 77 | 89 | 71 | 94 | 98 | 169 | 97 | 55 | 63 | 45 | 149 | 169 | 179 | 420 | 222 | 274 | 399 | 464 | 104 | 82 | 156 | 208 |
Labelled Cell Count | 403 | 483 | 84 | 92 | 76 | 102 | 109 | 196 | 118 | 63 | 79 | 57 | 150 | 170 | 192 | 484 | 256 | 310 | 399 | 464 | 104 | 82 | 156 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 3.8 | 2.9 | 3.1 | 3.2 | 3.1 | 2.9 | 3.0 | 2.6 | 2.9 | 3.0 | 2.7 | 4.4 | 4.9 | 4.2 | 6.5 | 7.3 | 7.0 | 4.0 | 4.8 | 5.2 |
Std Deviation (1e-4) | 0.9 | 0.7 | 0.2 | 0.8 | 0.3 | 1.1 | 1.0 | 0.7 | 0.7 | 1.5 | 1.2 | 0.7 | 1.5 | 1.6 | 1.6 | 2.5 | 3.3 | 3.0 | 0.9 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.16 | 0.08 | -0.0 | 0.03 | -0.16 | -0.02 | 0.03 | -0.09 | 0.6 | 0.75 | 0.54 | 1.17 | 1.34 | 1.28 | 0.45 | 0.72 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5626 | 1.4954 | 1.3747 | 1.2735 | 0.843 | 1.315 | 4.9959 | 3.5988 | 3.8982 | 3.8325 | 3.7947 | 3.9033 | 3.6126 | 3.8715 | 3.9026 | 4.3377 | 3.375 | 3.7312 |
Actin | 0.0083 | 0.0014 | 0.0143 | 0.0017 | 0.0016 | 0.0015 | 0.1162 | 0.0028 | 0.0365 | 0.0349 | 0.0054 | 0.01 | 0.0166 | 0.0262 | 0.0293 | 0.0016 | 0.0035 | 0.0042 |
Bud | 0.0005 | 0.0007 | 0.0138 | 0.0005 | 0.0002 | 0.0002 | 0.0044 | 0.0063 | 0.0172 | 0.0012 | 0.0011 | 0.007 | 0.0024 | 0.0137 | 0.0197 | 0.0001 | 0.0055 | 0.0002 |
Bud Neck | 0.0264 | 0.001 | 0.0007 | 0.0017 | 0.0016 | 0.2651 | 0.0247 | 0.0041 | 0.0017 | 0.0584 | 0.0005 | 0.1274 | 0.0169 | 0.0037 | 0.0025 | 0.0002 | 0.0172 | 0.1528 |
Bud Periphery | 0.0013 | 0.0015 | 0.0387 | 0.001 | 0.0005 | 0.0005 | 0.0143 | 0.0112 | 0.0629 | 0.0041 | 0.0022 | 0.0124 | 0.0142 | 0.0088 | 0.0287 | 0.0003 | 0.0142 | 0.0003 |
Bud Site | 0.0339 | 0.0421 | 0.0147 | 0.0003 | 0.0002 | 0.0042 | 0.0344 | 0.0519 | 0.0108 | 0.0011 | 0.0007 | 0.0013 | 0.0483 | 0.0964 | 0.0091 | 0.0001 | 0.0072 | 0.0006 |
Cell Periphery | 0.0058 | 0.0044 | 0.0027 | 0.0005 | 0.0006 | 0.0012 | 0.0033 | 0.0013 | 0.0014 | 0.0014 | 0.0004 | 0.0009 | 0.0086 | 0.0014 | 0.0009 | 0.0005 | 0.002 | 0.0006 |
Cytoplasm | 0.393 | 0.5155 | 0.4927 | 0.4626 | 0.7597 | 0.5176 | 0.1064 | 0.2829 | 0.2609 | 0.2552 | 0.2448 | 0.3346 | 0.4421 | 0.3469 | 0.3249 | 0.2463 | 0.3581 | 0.3722 |
Cytoplasmic Foci | 0.0087 | 0.0075 | 0.018 | 0.0069 | 0.0043 | 0.0094 | 0.043 | 0.0123 | 0.0159 | 0.032 | 0.0145 | 0.0125 | 0.0058 | 0.0149 | 0.0246 | 0.0052 | 0.0704 | 0.0112 |
Eisosomes | 0.0011 | 0.0007 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0022 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.004 | 0.0061 | 0.0048 | 0.0038 | 0.0013 | 0.0078 | 0.0021 | 0.0044 | 0.0021 | 0.0046 | 0.0015 | 0.0024 | 0.0009 | 0.0021 | 0.0038 | 0.0019 | 0.0019 |
Endosome | 0.0007 | 0.0013 | 0.0037 | 0.0034 | 0.0014 | 0.0009 | 0.0248 | 0.0019 | 0.0151 | 0.0029 | 0.048 | 0.0111 | 0.0101 | 0.0013 | 0.0025 | 0.0017 | 0.002 | 0.0005 |
Golgi | 0.0004 | 0.0004 | 0.0019 | 0.0003 | 0.0002 | 0.0003 | 0.0136 | 0.0005 | 0.007 | 0.0019 | 0.0152 | 0.0037 | 0.0015 | 0.0006 | 0.0022 | 0.0006 | 0.0003 | 0.0001 |
Lipid Particles | 0.0006 | 0.0005 | 0.0009 | 0.0006 | 0.0001 | 0.0001 | 0.0297 | 0.0004 | 0.008 | 0.0007 | 0.0013 | 0.0005 | 0.0004 | 0.0006 | 0.0012 | 0.0005 | 0.0001 | 0.0001 |
Mitochondria | 0.0002 | 0.0004 | 0.0026 | 0.0005 | 0.0002 | 0.0002 | 0.0215 | 0.0006 | 0.0068 | 0.0011 | 0.0278 | 0.0019 | 0.0012 | 0.0004 | 0.0157 | 0.0003 | 0.0003 | 0.0001 |
None | 0.5118 | 0.4147 | 0.3808 | 0.5078 | 0.2227 | 0.1958 | 0.4354 | 0.6139 | 0.5304 | 0.5955 | 0.6224 | 0.4656 | 0.4131 | 0.4786 | 0.5207 | 0.7363 | 0.5139 | 0.4538 |
Nuclear Periphery | 0.0003 | 0.0004 | 0.0008 | 0.001 | 0.0003 | 0.0001 | 0.0277 | 0.0004 | 0.0025 | 0.0014 | 0.0032 | 0.0013 | 0.0019 | 0.0004 | 0.0011 | 0.0005 | 0.0002 | 0.0001 |
Nucleolus | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0031 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0011 | 0.001 | 0.0012 | 0.0018 | 0.0009 | 0.0005 | 0.0119 | 0.0013 | 0.0017 | 0.0005 | 0.001 | 0.0036 | 0.0035 | 0.0016 | 0.0016 | 0.0004 | 0.0004 | 0.0003 |
Peroxisomes | 0.0003 | 0.0001 | 0.0028 | 0.0002 | 0.0001 | 0.0001 | 0.0506 | 0.001 | 0.01 | 0.0012 | 0.0006 | 0.0013 | 0.0002 | 0.0008 | 0.0102 | 0.0001 | 0.001 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.0006 | 0.0008 | 0.002 | 0.0003 | 0.0003 | 0.0136 | 0.0016 | 0.0013 | 0.0007 | 0.0008 | 0.0016 | 0.0016 | 0.0015 | 0.0011 | 0.0003 | 0.0009 | 0.0003 |
Vacuole | 0.0007 | 0.0014 | 0.0016 | 0.0011 | 0.0009 | 0.0004 | 0.0089 | 0.0023 | 0.0039 | 0.0024 | 0.003 | 0.0009 | 0.0072 | 0.0007 | 0.001 | 0.0006 | 0.0006 | 0.0002 |
Vacuole Periphery | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0026 | 0.0001 | 0.0008 | 0.0004 | 0.0023 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.5663 | 13.5684 | 13.1248 | 20.5397 | 24.4234 | 19.775 | 19.1086 | 21.2471 | 20.6712 | 24.9434 |
Translational Efficiency | 0.4657 | 0.3566 | 0.4358 | 0.2611 | 0.2544 | 0.4696 | 0.3717 | 0.3235 | 0.3576 | 0.3379 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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