Standard name
Human Ortholog
Description Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.07 0.08 0.1 0.07 0 0 0 0.18 0.2 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.18 0.05 0.21 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0.06 0.05 0.08 0.1 0.14 0.05 0 0 0.29 0.2 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0.91 0.94 0.95 0.88 0.86 0.88 0.86 0.81 0.83 0.7 0.69 0.68 0.96 0.95 0.91 0 0.05 0 0.93 0.94 0.96 0.92 0.91 0.87
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0.18 0.08 0 0 0.05 0 0.11 0 0 0 0 0 0.18 0.3 0.32 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.1 0.09 0 0 0 0.24 0.2 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.08 0 0 0 0.09 0 0.05 0.07 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.06 0.08 0.08 0 0.12 0.07 0.07 0 0 0 0.18 0.2 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 5 14 21 24 33 51 58 49 48 5 12 21 3 4 0 2 3 2 1 2 3
Bud 0 0 0 0 5 11 2 8 4 8 12 7 0 2 1 0 0 1 0 0 0 1 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 2 11 8 21 17 18 30 32 20 27 6 36 84 3 1 4 0 0 0 0 0 0
Cytoplasm 6 11 6 13 13 34 34 58 35 58 49 91 16 16 19 5 4 6 2 4 0 2 4 4
Endoplasmic Reticulum 150 257 243 326 462 558 783 733 574 540 332 448 287 625 659 0 1 0 178 301 267 280 320 367
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Golgi 4 3 14 66 44 16 26 45 23 81 18 20 3 26 17 3 6 6 0 2 1 6 3 7
Mitochondria 0 1 1 1 2 9 29 15 35 18 48 61 0 0 0 4 4 2 1 0 1 1 2 5
Nucleus 0 0 0 0 1 0 2 1 3 3 2 4 0 0 0 0 0 0 0 1 0 0 1 0
Nuclear Periphery 1 0 21 10 10 19 79 36 34 51 30 26 1 1 2 0 1 0 2 0 2 5 5 5
Nucleolus 1 0 0 0 0 2 8 13 3 9 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 3 4 7 44 37 76 69 30 94 35 47 1 2 6 3 4 2 2 3 0 2 3 13
Unique Cell Count 165 273 256 369 539 637 913 907 694 770 480 660 300 656 726 17 20 19 191 322 280 303 353 421
Labelled Cell Count 170 279 294 439 603 728 1080 1029 822 952 598 783 319 720 809 21 25 21 191 322 280 303 353 421


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 65.4 81.0 48.2 52.0 41.6 33.9 30.1 31.0 29.4 27.9 26.0 23.2 61.1 62.7 58.1 55.1 57.8 60.7 69.8 67.8 66.9
Std Deviation (1e-4) 15.3 17.3 11.6 11.4 8.8 7.4 6.7 7.1 6.6 6.9 6.6 7.0 13.6 11.9 11.8 16.9 21.7 19.8 14.5 16.9 17.2
Intensity Change (Log2) – – – 0.11 -0.21 -0.51 -0.68 -0.64 -0.71 -0.79 -0.89 -1.06 0.34 0.38 0.27 0.2 0.26 0.33 0.53 0.49 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.3 1.8 1.4 2.0 3.6 3.7 4.6 3.6 0 0 1.5 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.9 0 2.1 0 0 2.7 2.6 2.6 2.6 0 3.2 5.3 0 0 0
Cytoplasm 0.8 0.1 2.0 1.1 2.5 1.8 3.0 3.9 5.1 1.8 0.1 0.2 0 0 0
Endoplasmic Reticulum -2.8 -3.8 -3.3 -4.0 -5.4 -4.8 -8.1 -8.1 -8.5 0.4 0.2 -2.1 -12.1 -12.1 -12.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4.6 1.4 -2.2 -2.0 -0.3 -1.5 2.4 -1.1 -1.8 -3.1 -1.0 -2.5 0 0 0
Mitochondria 0 0 0 2.5 0 3.3 0 5.0 4.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.1 -4.3 -3.4 0.2 -2.8 -1.9 -0.9 -1.0 -2.6 -4.7 -7.1 -7.2 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 2.7 3.8 3.5 2.0 5.0 3.3 3.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 65.9688 70.141 71.2184 67.3158 55.7033 66.3363 66.3676 70.8526 72.3344 69.8926 68.5291 67.8082 79.3259 77.6529 83.3232 78.9075 80.851 75.9368
Actin 0.0004 0 0.0003 0.0003 0.0028 0.0001 0.0071 0.0001 0.0006 0.001 0.0003 0.0003 0.0003 0.0001 0.0008 0 0.0002 0.0005
Bud 0.0001 0 0 0 0.0002 0.0001 0.0002 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.0001 0 0.0001 0.0001 0.0021 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0004 0.0001 0.0001 0.0002 0.0007
Bud Periphery 0.0004 0 0.0001 0.0001 0.0012 0.0001 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Bud Site 0.0005 0 0.0002 0 0.0003 0 0.0003 0 0.0001 0 0 0 0 0 0 0 0 0
Cell Periphery 0.0191 0.0227 0.0362 0.0265 0.0369 0.0081 0.0138 0.0198 0.0208 0.0209 0.0182 0.002 0.0298 0.0426 0.0429 0.0232 0.0075 0.0082
Cytoplasm 0.0035 0.0013 0.0009 0.0009 0.0037 0.0014 0.0018 0.003 0.0009 0.0002 0.001 0.0002 0.0012 0.0102 0.0004 0.0002 0.0002 0.0003
Cytoplasmic Foci 0.0077 0 0.0057 0.0001 0.0092 0.0016 0.0223 0.0001 0.005 0.0039 0.0014 0 0 0 0 0 0 0
Eisosomes 0.0012 0 0.0018 0.0004 0.0032 0.0001 0.001 0.0012 0.0013 0.001 0.0163 0.0001 0.0021 0.0015 0.0017 0.0005 0.0006 0.0003
Endoplasmic Reticulum 0.754 0.9245 0.8828 0.8918 0.7506 0.8612 0.6296 0.8848 0.8902 0.8614 0.8542 0.7707 0.5768 0.8215 0.8471 0.9302 0.8067 0.7617
Endosome 0.0011 0 0.0005 0.0008 0.0011 0.0004 0.0023 0.0001 0.0014 0.0008 0.0017 0.0002 0.0005 0.0001 0.0002 0.0001 0.0005 0.0002
Golgi 0.0005 0.0001 0.0009 0.0008 0.0014 0.0003 0.0025 0.0006 0.0012 0.0017 0.0035 0.0008 0.0005 0.0004 0.0009 0.0006 0.0051 0.0011
Lipid Particles 0.0051 0.005 0.0058 0.0044 0.0116 0.0029 0.0164 0.0141 0.0013 0.0129 0.0092 0.0007 0.0012 0.0029 0.0013 0.0009 0.0011 0.0012
Mitochondria 0.0024 0 0.0023 0.0002 0.0341 0.0016 0.0211 0.0001 0.0026 0.0002 0.0017 0.0001 0.0002 0.0002 0.0003 0 0.0001 0.0001
None 0.003 0 0.0009 0.0003 0.0031 0.0017 0.0053 0.0001 0.0049 0.0001 0.0012 0 0.0002 0.0001 0.0001 0 0.0002 0.0001
Nuclear Periphery 0.1684 0.039 0.047 0.0607 0.0641 0.1029 0.2338 0.0675 0.0604 0.0706 0.0719 0.2158 0.3656 0.1048 0.0947 0.0403 0.169 0.2173
Nucleolus 0.0036 0 0.0017 0.0001 0.0151 0.0018 0.0033 0 0.0005 0 0.0015 0 0.0001 0 0 0 0 0
Nucleus 0.0039 0.0002 0.0004 0.0008 0.0023 0.0024 0.0039 0.0006 0.0004 0.0004 0.0016 0.001 0.0028 0.0006 0.0003 0.0001 0.0009 0.001
Peroxisomes 0.0002 0 0.002 0 0.0274 0 0.0015 0 0.0003 0.0193 0.0001 0 0 0 0 0 0 0
Punctate Nuclear 0.0024 0 0.0002 0 0.0004 0.0011 0.0037 0 0.0006 0.0002 0.0005 0 0 0 0 0 0 0
Vacuole 0.0151 0.0066 0.0064 0.0073 0.019 0.0065 0.0125 0.0055 0.005 0.0029 0.0037 0.002 0.0091 0.0123 0.0064 0.0028 0.0017 0.0026
Vacuole Periphery 0.0072 0.0004 0.0038 0.0043 0.0103 0.0054 0.017 0.0022 0.0022 0.0023 0.0119 0.0058 0.0092 0.0022 0.0025 0.0008 0.0058 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 314.651 206.2077 151.6048 179.2096 239.1245 182.7365 209.4572 141.3695 112.3831 261.9466
Translational Efficiency 1.0729 1.3953 1.176 0.8363 1.0278 1.9179 1.1111 0.957 1.2021 1.0436

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
Localization
Cell Percentages ER (96%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Elo3

Elo3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elo3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available