Standard name
Human Ortholog
Description Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 – 0 0 0.05 0 0 0.12 0 0.1 0 0 0 0 0 0
Bud 0.05 0 0.11 0 0.06 0 0.06 0 0.06 – 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.12 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.06 0 0.15 0.06 0.08 0.1 0 0.05 0.05 – 0 0 0.08 0.15 0.23 0.14 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0.07 0 0.1 0 0.06 0.05 0 – 0 0 0 0.19 0.18 0.08 0.11 0.09 0.1 0.09 0.1 0.07 0.09 0.08
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.15 0.21 0.14 0 0 0 0 0 0
Mitochondria 0.86 0.87 0.49 0.85 0.71 0.81 0.75 0.85 0.93 – 0.94 0.89 0.84 0.56 0.55 0.16 0.14 0.15 0.71 0.72 0.74 0.69 0.61 0.64
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0.05 0.06 0.09 0 0 0 0 – 0 0 0 0.1 0.08 0.07 0.07 0 0 0.05 0 0.07 0.09 0.06
SpindlePole 0.05 0 0.18 0.06 0.1 0.06 0.12 0.12 0 – 0.06 0.1 0 0 0 0.18 0.21 0.27 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.13 0.06 0 0.12 0.08 0.1 0.11 0.06 – 0.05 0.07 0.08 0.08 0.06 0.1 0.1 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 12 4 1 0 2 2 0 – 0 1 14 2 1 38 9 20 13 7 1 5 11 19
Bud 23 5 16 3 15 6 16 12 15 – 7 17 7 7 4 10 5 6 2 2 0 5 6 6
Bud Neck 4 0 5 0 1 1 1 0 0 – 0 0 0 0 0 2 0 0 4 1 0 5 14 11
Bud Site 5 1 0 0 0 0 4 4 4 – 0 3 0 0 0 0 4 1 – – – – – –
Cell Periphery 8 11 4 2 5 0 6 1 3 – 2 3 7 12 3 39 19 19 0 0 0 1 1 2
Cytoplasm 27 8 22 8 20 21 11 15 12 – 5 6 22 42 74 43 23 21 2 1 1 0 1 1
Endoplasmic Reticulum 7 10 5 3 3 0 0 1 2 – 2 1 8 4 5 19 9 13 3 0 2 4 4 7
Endosome 15 6 11 6 24 7 15 15 0 – 4 3 9 53 57 24 23 19 53 32 20 19 39 38
Golgi 2 1 1 1 1 1 0 2 0 – 0 0 5 0 3 46 43 28 15 8 3 5 8 6
Mitochondria 413 267 73 118 172 179 187 257 232 – 144 177 245 155 178 51 28 30 381 251 156 182 260 297
Nucleus 7 1 1 1 1 8 11 10 5 – 3 2 0 1 0 11 11 4 3 0 0 0 0 2
Nuclear Periphery 3 3 0 0 2 2 4 4 6 – 3 6 2 1 1 0 1 1 3 3 2 0 3 5
Nucleolus 5 1 0 0 0 2 4 5 11 – 4 2 0 0 0 1 1 1 1 2 0 1 2 2
Peroxisomes 9 14 7 9 21 7 10 7 0 – 3 4 12 28 26 22 14 6 18 16 8 17 39 27
SpindlePole 23 7 27 9 25 13 31 38 8 – 9 19 1 8 4 58 43 56 8 2 1 1 5 5
Vac/Vac Membrane 38 39 9 4 29 17 26 32 15 – 7 14 22 23 18 33 21 22 17 11 4 9 17 19
Unique Cell Count 480 307 149 139 242 221 249 304 250 154 200 293 279 324 315 204 204 535 348 211 266 424 464
Labelled Cell Count 594 380 193 168 320 264 328 405 313 193 258 354 336 374 397 254 247 535 348 211 266 424 464


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.8 5.7 5.1 5.8 4.8 5.6 5.1 4.4 – 4.3 4.7 5.0 5.3 5.2 8.0 8.0 7.6 5.0 5.1 5.2
Std Deviation (1e-4) 0.8 0.7 2.2 2.2 1.4 1.4 1.9 1.0 1.3 – 1.2 1.5 0.9 1.2 1.5 1.5 1.6 1.5 1.7 1.7 1.4
Intensity Change (Log2) – – – -0.17 0.0 -0.24 -0.05 -0.16 -0.38 – -0.43 -0.29 -0.21 -0.12 -0.15 0.49 0.49 0.41 -0.2 -0.18 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.6 1.4 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9714 3.6308 3.3582 3.182 3.2203 3.4155 1.0834 1.7884 1.3328 1.0945 1.2555 1.43 1.4104 1.9635 1.7584 1.6213 1.7975 1.7179
Actin 0.0248 0.0092 0.0094 0.0013 0.0352 0.0018 0.0312 0.0467 0.0247 0.0186 0.0145 0.0259 0.0193 0.0293 0.029 0.006 0.0508 0.0291
Bud 0.0004 0.0035 0.0012 0.0002 0.0004 0.0005 0.0011 0.0031 0.001 0.0011 0.0043 0.0006 0.0012 0.003 0.0046 0.0007 0.007 0.0034
Bud Neck 0.0009 0.0008 0.0076 0.0005 0.0003 0.0011 0.0012 0.0014 0.0012 0.0002 0.001 0.0017 0.0034 0.0019 0.0031 0.0002 0.0037 0.0042
Bud Periphery 0.0014 0.0078 0.0044 0.0009 0.0009 0.003 0.0015 0.0053 0.0019 0.0074 0.0106 0.0012 0.0019 0.0032 0.0062 0.0019 0.0178 0.0092
Bud Site 0.005 0.0133 0.0086 0.0005 0.0015 0.0005 0.0035 0.0224 0.0056 0.0007 0.0087 0.0009 0.01 0.0376 0.0313 0.0006 0.0096 0.003
Cell Periphery 0.0015 0.0006 0.004 0.0018 0.0002 0.0046 0.0007 0.0012 0.0009 0.0009 0.0005 0.0003 0.0009 0.0009 0.0014 0.0004 0.0008 0.0008
Cytoplasm 0.0063 0.0047 0.0021 0.0063 0.0007 0.003 0.0077 0.0092 0.0044 0.0005 0.0009 0.0048 0.0056 0.0063 0.011 0.0018 0.0037 0.0074
Cytoplasmic Foci 0.0276 0.0619 0.0102 0.0153 0.0098 0.0342 0.0596 0.0563 0.0396 0.0081 0.0278 0.0336 0.0832 0.0714 0.0885 0.0131 0.0866 0.0809
Eisosomes 0.0021 0.0022 0.0008 0.001 0.0004 0.0003 0.0006 0.0008 0.0004 0.0005 0.0003 0.0004 0.0007 0.0006 0.0009 0.0003 0.0006 0.0004
Endoplasmic Reticulum 0.0074 0.0005 0.0003 0.0121 0.0011 0.001 0.0052 0.0007 0.0026 0.0001 0.0009 0.0008 0.0032 0.0013 0.0017 0.0025 0.0026 0.0013
Endosome 0.0473 0.0523 0.0262 0.0601 0.0143 0.0152 0.1023 0.0604 0.0543 0.0162 0.0709 0.0431 0.0677 0.0576 0.0467 0.0472 0.0582 0.0427
Golgi 0.0374 0.0787 0.0187 0.0458 0.0122 0.0184 0.0846 0.0673 0.0537 0.0236 0.1141 0.08 0.0444 0.0626 0.0516 0.0198 0.033 0.0517
Lipid Particles 0.0527 0.0336 0.0162 0.0296 0.0114 0.0291 0.0312 0.0332 0.0104 0.0023 0.0527 0.0196 0.1133 0.0213 0.0331 0.0337 0.0508 0.0606
Mitochondria 0.6717 0.6255 0.8365 0.7251 0.8475 0.8238 0.5821 0.6059 0.7238 0.8887 0.6504 0.7331 0.5158 0.5698 0.5601 0.8303 0.5309 0.6009
None 0.0164 0.0058 0.0102 0.0365 0.0025 0.0035 0.0068 0.007 0.0012 0.0003 0.0004 0.0055 0.0232 0.0076 0.0206 0.0019 0.0201 0.0139
Nuclear Periphery 0.0062 0.0003 0.0002 0.0023 0.0016 0.0002 0.0032 0.0004 0.0029 0 0.0006 0.0004 0.0025 0.0004 0.0034 0.0104 0.0147 0.0011
Nucleolus 0.0038 0.0007 0.0011 0.0004 0.0002 0.0004 0.0049 0.0005 0.0007 0 0.0038 0.0005 0.0022 0.0018 0.0033 0.0007 0.0027 0.001
Nucleus 0.0022 0.0003 0.0005 0.0005 0.0003 0.0003 0.0011 0.0007 0.0006 0.0001 0.0006 0.0002 0.0013 0.0007 0.0019 0.0012 0.018 0.001
Peroxisomes 0.0712 0.0921 0.0311 0.0496 0.0553 0.0536 0.0583 0.0704 0.061 0.0291 0.0301 0.0436 0.0854 0.1126 0.0904 0.0161 0.0725 0.0779
Punctate Nuclear 0.001 0.0006 0.0005 0.0002 0.0024 0.0009 0.0019 0.0008 0.0004 0 0.0005 0.0004 0.0028 0.0011 0.004 0.0022 0.0071 0.0035
Vacuole 0.0081 0.0034 0.0054 0.0052 0.0007 0.0023 0.0077 0.0053 0.0057 0.0006 0.0036 0.0015 0.0074 0.0058 0.0045 0.0042 0.0041 0.0034
Vacuole Periphery 0.0045 0.0021 0.0047 0.0048 0.0011 0.0022 0.0039 0.0015 0.0028 0.0009 0.0029 0.002 0.0044 0.0033 0.0026 0.0049 0.0046 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7942 7.7147 12.267 13.4759 11.9884 13.1432 12.9076 13.0363 15.3109 19.4012
Translational Efficiency 0.4262 0.5593 0.4014 0.4917 0.4367 0.6199 0.5218 0.4263 0.4984 0.3998

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Nam2

Nam2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nam2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available