Standard name
Human Ortholog
Description Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0.07 0.05 0.05 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.94 0.96 0.92 0.88 0.79 0.71 0.74 0.7 0.7 0.62 0.99 1.0 0.98 0.92 0.91 0.95 0.91 0.92 0.91 0.9 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0.06 0.07 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.09 0.21 0.24 0.28 0.34 0.35 0.37 0.53 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.09 0 0 0 0.07 0 0 0 0.06 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 3 3 10 2 4 2 3 0 0 1 1 0 0 0 0 0 0 0 1
Bud 1 0 10 4 4 8 3 10 6 15 8 22 1 0 1 0 0 0 1 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 12 19 2 10 15 13 11 18 24 25 24 4 12 9 17 24 18 0 0 1 0 0 0
Cytoplasm 187 458 419 230 324 324 402 361 316 323 246 341 156 178 172 295 261 246 193 452 433 108 178 184
Endoplasmic Reticulum 5 16 14 0 5 4 8 15 13 15 10 5 1 0 0 14 13 17 6 12 14 7 14 21
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 0 0 2 1 0 0 0
Mitochondria 0 2 8 25 33 76 124 141 146 159 128 288 0 1 9 4 2 3 3 4 7 0 1 1
Nucleus 0 1 0 0 2 4 6 6 0 2 4 3 1 0 0 5 3 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 1 0 0 1 0 0 1 3 1 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 7 1 3 1 23 48 7 8 9 41 0 0 0 18 17 7 2 9 15 3 1 2
Unique Cell Count 196 475 447 239 352 370 510 512 425 459 350 547 157 178 176 321 287 259 212 493 479 120 198 215
Labelled Cell Count 203 492 478 262 383 435 582 604 508 551 433 731 164 191 192 358 324 291 212 493 479 120 198 215


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.1 15.5 8.1 7.5 7.3 6.3 5.9 5.8 5.3 5.4 5.1 4.8 12.7 11.6 9.7 19.4 21.0 20.7 16.1 16.7 17.1
Std Deviation (1e-4) 3.3 2.8 1.2 1.2 1.3 1.2 1.1 1.1 1.1 1.2 1.2 1.1 1.8 1.6 1.4 4.8 5.2 5.1 3.1 3.2 3.0
Intensity Change (Log2) – – – -0.12 -0.15 -0.37 -0.47 -0.49 -0.63 -0.58 -0.68 -0.76 0.64 0.51 0.26 1.25 1.37 1.35 0.99 1.04 1.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.5 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.2 -0.1 -2.2 -0.3 -0.9 0.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -1.1 -0.1 -1.5 -1.9 0 0.7 1.8 0 -1.0 1.3 0.5 0.7 2.3 1.6
Cytoplasm 1.4 -0.9 -3.1 -6.6 -9.2 -7.9 -9.1 -8.8 -11.6 2.8 3.4 2.0 -1.0 -1.4 0.7
Endoplasmic Reticulum 0 -1.6 -2.0 -1.6 -0.2 -0.1 0.1 -0.2 0 0 0 0 0.9 1.0 2.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.1 4.8 8.8 10.1 11.0 12.6 12.7 13.0 0 0 0 0 -0.6 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.6 5.2 0.1 0.2 1.0 4.3 0 0 0 3.1 3.2 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.775 17.0759 14.7399 13.8871 13.4868 15.0085 12.6242 17.0921 15.4705 13.4668 14.859 16.1053 15.1875 18.4086 17.2862 16.2635 16.3383 16.9735
Actin 0.0014 0.0012 0.0007 0.0008 0.0005 0.0013 0.0244 0.0003 0.015 0.0056 0.0021 0.0036 0.0102 0.0012 0.0007 0.0007 0.0036 0.0008
Bud 0.0007 0.0007 0.0013 0.0012 0.0006 0.001 0.001 0.0002 0.0015 0.0011 0.0004 0.0004 0.0057 0.0057 0.001 0.0005 0.0123 0.0008
Bud Neck 0.001 0.0008 0.001 0.0014 0.0016 0.0048 0.0013 0.0003 0.0018 0.0014 0.0005 0.0015 0.0007 0.0003 0.0003 0.0005 0.0006 0.0008
Bud Periphery 0.0008 0.0005 0.0011 0.0015 0.0003 0.0016 0.0011 0.0001 0.0021 0.0008 0.0003 0.0007 0.0031 0.0013 0.0002 0.0002 0.0075 0.0003
Bud Site 0.0009 0.003 0.0013 0.0009 0.0002 0.0003 0.0075 0.0006 0.0049 0.0023 0.0003 0.0004 0.0072 0.0096 0.0005 0.0003 0.0009 0.0001
Cell Periphery 0.002 0.002 0.0013 0.0013 0.0003 0.0012 0.0012 0.0004 0.001 0.0003 0.0001 0.0014 0.0008 0.0003 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.7731 0.9464 0.8775 0.7831 0.8579 0.8229 0.7264 0.9624 0.864 0.7538 0.8681 0.8706 0.8392 0.9617 0.9506 0.8826 0.9076 0.9334
Cytoplasmic Foci 0.0032 0.0032 0.005 0.0054 0.0049 0.0066 0.0231 0.0061 0.009 0.0326 0.0089 0.0124 0.0211 0.0049 0.0092 0.0184 0.0285 0.0113
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0003 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.1169 0.0187 0.0412 0.0326 0.0557 0.1004 0.0593 0.0099 0.0254 0.0188 0.0043 0.047 0.0355 0.0025 0.0101 0.0046 0.0048 0.0189
Endosome 0.0217 0.0063 0.0166 0.0333 0.0317 0.0218 0.0511 0.0093 0.0279 0.0781 0.0783 0.0314 0.0333 0.0056 0.0167 0.0551 0.0185 0.0203
Golgi 0.0018 0.0009 0.0013 0.0014 0.0011 0.0026 0.01 0.001 0.0059 0.0201 0.0159 0.008 0.0107 0.0014 0.003 0.0128 0.0034 0.0039
Lipid Particles 0.0004 0.0004 0.0002 0.0004 0.0001 0.0004 0.0085 0.0001 0.0018 0.0113 0.0017 0.0013 0.0062 0.0001 0.0001 0.0025 0.0012 0.0001
Mitochondria 0.0096 0.0013 0.0056 0.0197 0.0041 0.0084 0.0096 0.0005 0.0173 0.0053 0.0067 0.0056 0.0051 0.0003 0.0009 0.0024 0.0004 0.0029
None 0.001 0.0003 0.0004 0.0004 0.0004 0.0005 0.0088 0.0004 0.0015 0.0017 0.0007 0.0008 0.0023 0.0005 0.0004 0.0006 0.0017 0.0016
Nuclear Periphery 0.0133 0.002 0.006 0.0083 0.005 0.0051 0.0117 0.0008 0.0041 0.004 0.0039 0.0041 0.0039 0.0004 0.0006 0.0009 0.001 0.0006
Nucleolus 0.0001 0 0.0001 0.0001 0 0 0.001 0 0.0002 0.0002 0 0.0001 0.0001 0.0001 0 0 0.0001 0
Nucleus 0.0038 0.0017 0.0028 0.0035 0.003 0.0026 0.01 0.0009 0.0018 0.0026 0.0011 0.0019 0.0016 0.001 0.0007 0.0009 0.0011 0.0008
Peroxisomes 0 0 0.0001 0.0001 0 0.0001 0.0061 0.0001 0.0005 0.0103 0.0003 0.0008 0.0042 0 0.0002 0.0006 0.0004 0.0001
Punctate Nuclear 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0087 0.0001 0.0004 0.0028 0.0003 0.0007 0.0009 0.0001 0.0001 0.0001 0.0013 0.0001
Vacuole 0.0408 0.0096 0.0323 0.0858 0.0256 0.0143 0.0253 0.006 0.0109 0.0406 0.0035 0.0061 0.0065 0.0029 0.0037 0.0131 0.0036 0.0022
Vacuole Periphery 0.0073 0.001 0.0043 0.0187 0.0069 0.004 0.0035 0.0005 0.0028 0.0063 0.0026 0.0012 0.0015 0.0004 0.0008 0.0031 0.001 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.8575 84.9988 102.6514 105.8623 101.909 72.975 68.8277 77.6173 82.0289 87.8054
Translational Efficiency 0.8376 0.9594 0.9053 0.9324 0.7339 0.772 0.8375 0.6117 0.632 0.677

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Iki3

Iki3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iki3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available