Standard name
Human Ortholog
Description Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 – 0 0.1 0.1 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.13 0.11 0.2 0.22 0.22 0.23 – 0.2 0.21 0.16 0.27 0.1 0.12 0.2 0.14 0.12 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.1 – 0.05 0 0.05 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.31 0.16 0.38 0.24 0.31 0.2 0.09 – 0.07 0 0 0.07 0.24 0.34 0.26 0.15 0.27 0.23 0.52 0.39 0.48 0.47 0.46 0.55
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Mitochondria 0.09 0.09 0 0.24 0.07 0.36 0.46 – 0.65 0.65 0.78 0.6 0.16 0 0.05 0.07 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0.06 0.05
Nucleolus 0.05 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.54 0.71 0.66 0.38 0.53 0.34 0.24 – 0.18 0.2 0.16 0.17 0.69 0.72 0.62 0.73 0.76 0.76 0.28 0.5 0.36 0.39 0.38 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 2 0 9 – 0 3 3 4 0 0 1 3 3 1 2 2 1 1 1 2
Bud 1 1 1 3 1 6 15 – 9 21 22 13 3 3 1 0 0 0 1 1 2 0 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 2 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 27 34 26 17 46 43 64 – 49 43 34 57 26 40 67 18 25 16 1 0 1 1 2 1
Endoplasmic Reticulum 9 7 4 2 3 9 29 – 12 8 10 7 2 15 8 1 5 7 3 0 3 2 5 4
Endosome 68 40 88 21 65 40 24 – 16 2 3 14 59 108 88 19 54 29 127 118 126 113 153 162
Golgi 3 1 2 0 1 0 3 – 2 0 0 2 0 0 0 3 16 6 7 5 4 2 3 7
Mitochondria 19 24 4 21 14 70 129 – 161 133 167 124 40 12 17 9 3 5 9 11 11 8 9 11
Nucleus 3 1 1 1 4 3 4 – 2 7 1 2 0 1 4 3 1 1 1 1 0 1 2 1
Nuclear Periphery 6 6 2 0 1 2 3 – 7 3 2 1 2 1 1 3 0 0 14 6 10 11 20 15
Nucleolus 11 7 3 5 5 4 1 – 1 3 1 0 2 10 5 5 9 8 1 0 0 0 1 1
Peroxisomes 0 0 1 0 0 0 0 – 1 0 0 0 0 3 1 0 0 0 0 1 0 0 1 0
SpindlePole 5 3 10 9 9 2 3 – 0 2 4 5 1 6 5 0 5 4 3 1 2 1 3 3
Vac/Vac Membrane 117 182 152 33 111 66 67 – 44 42 35 35 171 231 207 95 154 96 68 151 93 94 126 78
Unique Cell Count 217 258 230 87 210 197 279 246 205 214 208 249 320 333 130 202 126 246 305 261 242 336 297
Labelled Cell Count 269 306 297 113 262 245 352 304 269 285 264 306 430 405 159 275 173 246 305 261 242 336 297


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.1 7.5 6.2 7.0 5.9 5.8 – 5.4 4.9 4.9 5.4 6.3 6.8 7.0 10.8 11.9 12.0 6.2 6.5 6.9
Std Deviation (1e-4) 1.4 1.3 1.6 1.5 1.5 1.4 1.3 – 1.2 1.0 1.1 1.1 1.0 1.3 1.3 2.5 2.7 2.5 1.3 1.6 1.5
Intensity Change (Log2) – – – -0.28 -0.1 -0.34 -0.37 – -0.46 -0.62 -0.61 -0.46 -0.25 -0.13 -0.09 0.53 0.67 0.68 -0.27 -0.21 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.4 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 3.2 – 0 0 4.7 3.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 3.0 2.9 3.4 – 2.6 2.8 1.4 4.3 -0.3 0.4 2.8 0.7 0.3 0.4
Endoplasmic Reticulum 0 0 1.7 3.9 – 1.9 0 1.8 1.1 0 1.9 0 0 0 0
Endosome -2.4 -1.6 -4.0 -8.0 – -8.4 0 -9.6 -7.8 -3.5 -1.1 -3.0 -4.7 -2.5 -2.9
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 3.7 0
Mitochondria 6.6 2.6 9.2 11.4 – 14.6 0 16.5 13.3 5.4 1.4 2.1 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 1.4 0 0 2.0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.0 0 -2.1 -2.3 – 0 0 -1.5 -1.1 -2.9 -1.7 -2.1 0 -1.1 0
Vacuole -4.5 -2.8 -6.7 -9.5 – -10.7 -9.6 -10.6 -10.4 0.6 1.5 -1.0 1.4 2.3 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1435 4.6287 4.5996 3.7414 4.0252 4.2936 2.5715 2.9904 3.0112 2.54 2.9657 2.5813 2.334 2.1971 2.5192 2.8047 1.9759 2.3294
Actin 0.0134 0.0003 0.0058 0.0001 0.0033 0.0015 0.0122 0.0001 0.0142 0 0.0187 0.0006 0.0125 0.0003 0.0062 0.0232 0 0.0115
Bud 0.0017 0.0044 0.0009 0.0004 0.0026 0.0022 0.0005 0.0009 0.0015 0.0001 0.0045 0.0005 0.0004 0.0004 0.0009 0.0004 0.0001 0.0004
Bud Neck 0.0022 0.0003 0.0012 0.0027 0.0002 0.0015 0.0007 0.0005 0.0005 0.0001 0.0008 0.0005 0.0013 0.0003 0.0037 0.0009 0.0001 0.001
Bud Periphery 0.0017 0.007 0.0016 0.0008 0.0068 0.0058 0.0008 0.0012 0.0018 0.0001 0.0041 0.0014 0.0004 0.0002 0.0008 0.0005 0.0001 0.0008
Bud Site 0.0021 0.0022 0.0077 0.0005 0.0006 0.001 0.0014 0.0015 0.002 0.0001 0.0086 0.0003 0.0018 0.0019 0.0025 0.001 0.0001 0.0006
Cell Periphery 0.0008 0.0003 0.0005 0.0005 0.0001 0.0004 0.0003 0.0004 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0 0.0002
Cytoplasm 0.0185 0.0475 0.0195 0.0047 0.0078 0.0172 0.0079 0.0292 0.0305 0.0037 0.0078 0.0061 0.014 0.0232 0.0245 0.0065 0.0028 0.0144
Cytoplasmic Foci 0.0438 0.0289 0.0361 0.0237 0.0273 0.0323 0.0442 0.0366 0.0391 0.0087 0.045 0.0311 0.0483 0.0232 0.0466 0.0164 0.0132 0.0249
Eisosomes 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0 0.0004 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.004 0.0041 0.0059 0.0019 0.0009 0.0055 0.0062 0.0046 0.0031 0.0033 0.0023 0.0033 0.0046 0.0046 0.0023 0.0012 0.001 0.0038
Endosome 0.534 0.5797 0.5424 0.5444 0.6175 0.5546 0.5691 0.5662 0.5847 0.5795 0.6095 0.6102 0.4897 0.5547 0.5414 0.5967 0.6267 0.5326
Golgi 0.0276 0.0208 0.0403 0.0186 0.0559 0.0323 0.0392 0.027 0.0417 0.0108 0.0858 0.0283 0.0217 0.0135 0.0335 0.0256 0.0236 0.0282
Lipid Particles 0.0432 0.0072 0.0282 0.0057 0.0483 0.006 0.0383 0.0087 0.0103 0.0024 0.0145 0.014 0.0234 0.0049 0.0172 0.0047 0.0012 0.0081
Mitochondria 0.023 0.0101 0.0386 0.0124 0.0195 0.0344 0.0164 0.0075 0.025 0.004 0.0363 0.0176 0.0133 0.0019 0.0126 0.006 0.0013 0.007
None 0.0095 0.0118 0.0197 0.0006 0.0002 0.003 0.0028 0.0012 0.0005 0.0002 0.0003 0.0003 0.0222 0.0015 0.0063 0.0003 0.0001 0.0007
Nuclear Periphery 0.0152 0.0118 0.0089 0.0048 0.0024 0.0104 0.0174 0.0087 0.0041 0.0131 0.0025 0.0067 0.0121 0.0107 0.01 0.0042 0.0023 0.0118
Nucleolus 0.0035 0.0006 0.0009 0.0005 0.0001 0.0007 0.0007 0.0007 0.001 0.0001 0.0003 0.0012 0.003 0.0003 0.0018 0.0004 0 0.001
Nucleus 0.0046 0.0021 0.0031 0.0008 0.0006 0.0025 0.0018 0.0012 0.0013 0.0006 0.0009 0.0012 0.0026 0.0013 0.0021 0.0006 0.0002 0.002
Peroxisomes 0.011 0.001 0.0049 0.0007 0.0075 0.0021 0.0178 0.0011 0.003 0.0001 0.0074 0.0016 0.0129 0.0012 0.0065 0.0045 0.0002 0.0011
Punctate Nuclear 0.0016 0.0003 0.0011 0.0001 0.0001 0.0006 0.003 0.0002 0.0045 0 0.0007 0.0004 0.0042 0.0002 0.0026 0.0011 0 0.0028
Vacuole 0.1718 0.1923 0.1668 0.2418 0.1382 0.1823 0.1592 0.2102 0.1593 0.2167 0.0979 0.1795 0.2225 0.2515 0.1907 0.1786 0.1776 0.2185
Vacuole Periphery 0.0664 0.0674 0.0656 0.1344 0.06 0.104 0.0601 0.0921 0.0717 0.1564 0.0516 0.0951 0.0886 0.1042 0.0873 0.1271 0.1494 0.1287

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.3451 14.1817 12.7389 18.1159 25.711 20.4453 18.5843 14.1349 17.6149 30.9404
Translational Efficiency 1.2075 1.1925 0.8767 0.8241 1.0008 1.6144 0.7799 0.7989 0.8856 0.9322

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
Localization
Cell Percentages vacuolar membrane (37%), endosome (10%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vac14

Vac14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vac14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available