Standard name
Human Ortholog
Description Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.96 0.96 0.87 0.8 0.73 0.78 0.75 0.69 0.65 0.63 0.99 0.98 0.98 0.94 0.95 0.97 0.95 0.96 0.93 0.96 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.25 0.32 0.22 0.26 0.38 0.4 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.07 0.05 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 2 4 5 4 0 6 3 7 0 1 0 0 0 0 0 0 1 0 1 1
Bud 2 1 3 2 3 9 36 10 15 24 11 20 0 0 0 0 0 0 0 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 1 28 8 19 23 24 39 32 35 35 40 12 24 7 12 16 6 0 0 1 0 0 0
Cytoplasm 221 340 606 374 414 517 538 565 389 449 365 383 447 665 555 289 361 345 223 326 609 437 526 493
Endoplasmic Reticulum 1 2 10 3 9 12 7 10 15 8 5 11 2 15 12 8 12 7 0 1 17 0 2 6
Endosome 1 0 1 0 0 1 5 4 3 2 0 3 2 1 0 1 0 1 0 2 2 3 1 3
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 1 0 1 2 1 0 0 1 0 1 0 0
Mitochondria 2 2 5 13 48 162 235 158 138 248 223 261 0 0 0 2 3 1 1 1 3 1 1 2
Nucleus 1 1 1 1 2 2 3 1 0 3 0 3 0 2 1 7 3 5 1 0 1 1 2 1
Nuclear Periphery 0 0 1 0 0 0 1 0 2 1 0 1 0 0 0 0 0 0 0 0 2 0 0 4
Nucleolus 1 1 0 0 0 0 2 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 2 0 18 7 19 24 53 51 25 42 32 34 0 4 0 11 10 12 3 2 7 2 4 2
Unique Cell Count 230 344 633 390 477 643 741 723 522 654 561 606 450 677 565 308 379 354 235 339 657 457 546 526
Labelled Cell Count 237 349 674 408 516 754 909 842 621 818 675 763 464 713 576 332 406 377 235 339 657 457 546 526


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.9 12.6 12.6 11.4 10.4 8.5 7.7 8.0 6.8 6.7 6.2 6.1 14.7 14.6 13.8 17.5 16.0 15.4 14.7 14.9 16.1
Std Deviation (1e-4) 2.0 2.3 2.1 1.7 1.6 1.8 1.6 1.7 1.3 1.5 1.6 1.5 2.3 2.0 2.0 4.9 3.6 4.0 2.6 2.7 3.3
Intensity Change (Log2) – – – -0.14 -0.27 -0.56 -0.71 -0.65 -0.88 -0.91 -1.02 -1.04 0.23 0.22 0.14 0.48 0.35 0.3 0.22 0.25 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 4.9 1.7 3.3 4.0 2.4 3.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.0 -0.4 -0.8 -1.1 0.8 1.3 0.8 1.4 1.7 -1.5 -0.8 -3.3 -0.4 -0.2 -2.3
Cytoplasm 0.1 -5.4 -8.4 -11.4 -9.4 -10.4 -12.6 -13.6 -14.3 3.6 2.7 2.5 -1.3 -0.4 1.4
Endoplasmic Reticulum 0 0.4 0.4 -1.1 -0.3 1.5 -0.5 -1.1 0.3 0 0.8 0.7 1.1 1.7 0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 7.2 12.9 15.0 11.9 13.2 16.8 17.1 18.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 1.0 0.9 3.6 3.5 1.7 3.0 2.5 2.4 -3.6 -3.2 -4.0 0.6 -0.2 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8698 13.3069 12.7785 12.2312 11.725 12.9753 7.1523 11.9897 11.1002 11.2567 9.8522 11.2095 9.9754 13.0727 11.9885 11.467 10.9115 11.7253
Actin 0.0096 0.0085 0.0022 0.0001 0.0004 0.0011 0.0251 0.0074 0.0011 0.0025 0.0006 0.0027 0.0153 0.0006 0.0103 0.0021 0.0054 0.0022
Bud 0.0003 0.0003 0.0005 0.0003 0.0005 0.0002 0.0012 0.0012 0.0002 0.0063 0.0002 0.0024 0.0014 0.0036 0.0044 0.004 0.0097 0.0017
Bud Neck 0.0009 0.0002 0.0005 0.0003 0.0005 0.0008 0.0047 0.0001 0.0004 0.0007 0.0003 0.0009 0.0006 0.0005 0.0016 0.0005 0.0012 0.0017
Bud Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0012 0.0002 0.0003 0.0027 0.0001 0.001 0.001 0.0005 0.0017 0.0011 0.0057 0.0007
Bud Site 0.0008 0.0009 0.0131 0.0002 0.0002 0.0001 0.023 0.0007 0.0002 0.0039 0.0002 0.0004 0.0031 0.0015 0.0052 0.0014 0.0089 0.0021
Cell Periphery 0.0005 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0 0.0005 0 0.0001 0.0002 0.0002 0.0004 0.0002 0.0006 0.0001
Cytoplasm 0.7538 0.9532 0.908 0.9279 0.9549 0.9131 0.6293 0.9733 0.9604 0.8664 0.8381 0.9041 0.8405 0.9602 0.9168 0.9139 0.9057 0.9259
Cytoplasmic Foci 0.0309 0.0062 0.0133 0.027 0.0096 0.0089 0.0479 0.0042 0.0064 0.0185 0.0127 0.0129 0.0257 0.0064 0.0115 0.0087 0.0156 0.0105
Eisosomes 0 0.0001 0 0 0 0 0.0004 0 0 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0753 0.0038 0.023 0.0054 0.0031 0.0422 0.0335 0.0025 0.0026 0.0031 0.0013 0.0213 0.0204 0.0058 0.0073 0.0048 0.0044 0.0164
Endosome 0.0252 0.0084 0.0182 0.026 0.0202 0.0225 0.0532 0.0027 0.0078 0.0416 0.0662 0.0266 0.0292 0.0114 0.0134 0.0298 0.0193 0.0222
Golgi 0.0113 0.0024 0.0029 0.0011 0.0023 0.0028 0.0096 0.0011 0.0006 0.0008 0.0203 0.0035 0.0081 0.0015 0.0036 0.0065 0.0071 0.0039
Lipid Particles 0.0021 0.0027 0.0039 0.0003 0 0.0001 0.0249 0.0001 0.0001 0.0006 0.0171 0.0001 0.0081 0.0002 0.0034 0.0003 0.0003 0.0002
Mitochondria 0.0027 0.0021 0.0004 0.0003 0.001 0.0018 0.008 0.0004 0.0145 0.0007 0.0178 0.0034 0.0102 0.0002 0.0042 0.0045 0.0039 0.006
None 0.0081 0.0017 0.0009 0.0019 0.0007 0.0009 0.0135 0.0019 0.0007 0.0025 0.0004 0.0053 0.0053 0.0008 0.0016 0.0015 0.0014 0.0006
Nuclear Periphery 0.0222 0.0008 0.0022 0.0009 0.0007 0.0012 0.0385 0.0005 0.0007 0.0014 0.0011 0.0022 0.0065 0.001 0.0019 0.0021 0.001 0.0013
Nucleolus 0.0001 0.0001 0 0 0 0 0.0077 0 0 0.0002 0.0001 0.0003 0.0014 0.0002 0.0003 0.0002 0.0003 0
Nucleus 0.0442 0.0012 0.0013 0.0011 0.0013 0.0011 0.0289 0.0007 0.0007 0.0019 0.0011 0.0073 0.0029 0.002 0.0021 0.0021 0.0025 0.0009
Peroxisomes 0.0011 0.0026 0.0032 0.0001 0.0001 0 0.0257 0.001 0.0002 0.0003 0.0046 0.0002 0.0047 0.0001 0.0041 0.0001 0.0006 0.0002
Punctate Nuclear 0.0073 0.0005 0.0017 0.0007 0.0001 0.0001 0.0142 0.0001 0.0002 0.0004 0.0001 0.0012 0.0056 0.0005 0.0013 0.0003 0.0007 0.0001
Vacuole 0.0026 0.0036 0.0036 0.0051 0.003 0.0021 0.0059 0.0014 0.0021 0.0355 0.0125 0.003 0.0068 0.0024 0.0039 0.0139 0.0045 0.002
Vacuole Periphery 0.0007 0.0004 0.0007 0.001 0.0011 0.0008 0.0028 0.0002 0.0005 0.0096 0.0053 0.0011 0.0029 0.0003 0.0009 0.0021 0.0011 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.842 37.9668 43.9498 48.3064 34.1409 52.6353 50.0965 58.2962 49.2292 45.4758
Translational Efficiency 1.9992 2.2992 1.6319 1.6347 2.014 2.0826 1.7706 1.3511 1.6194 1.4554

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Reh1

Reh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Reh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available