Standard name
Human Ortholog
Description Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.98 0.97 0.95 0.88 0.85 0.81 0.69 0.74 0.65 0.63 1.0 0.99 1.0 0.97 0.97 0.97 0.89 0.86 0.76 0.87 0.85 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0.08 0.23 0.24 0.29 0.5 0.46 0.57 0.55 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 2 2 6 21 8 11 16
Bud 2 3 5 1 0 4 8 6 9 16 16 19 2 1 0 0 0 0 0 2 5 1 9 19
Bud Neck 0 0 0 0 1 1 1 2 0 2 1 0 0 0 0 3 0 1 3 4 10 2 3 10
Bud Site 0 0 0 0 0 1 0 0 1 1 0 3 0 1 0 0 0 0
Cell Periphery 1 8 11 1 6 6 8 3 8 12 9 11 18 41 46 7 5 9 2 4 9 4 10 14
Cytoplasm 205 328 414 215 323 373 503 360 268 441 309 348 401 553 482 581 332 299 195 295 319 166 318 335
Endoplasmic Reticulum 3 5 2 1 0 2 2 0 3 0 7 5 0 2 0 43 28 18 4 6 12 0 6 8
Endosome 0 0 0 0 0 4 5 3 0 0 2 1 0 0 0 3 3 1 1 3 2 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 0 2 0 0 0
Mitochondria 13 3 5 7 28 100 140 130 192 272 269 303 2 0 0 9 1 1 2 7 21 3 6 10
Nucleus 0 1 2 1 0 3 5 4 0 5 2 2 0 0 1 2 2 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
SpindlePole 1 0 0 0 1 1 2 1 0 1 1 4 0 0 0 2 1 1 0 1 1 0 2 4
Vac/Vac Membrane 1 2 0 1 5 2 13 4 9 11 6 11 0 2 0 8 12 5 2 6 2 1 0 12
Unique Cell Count 216 338 422 221 340 426 592 446 387 596 474 550 403 557 483 597 343 309 219 343 420 192 376 451
Labelled Cell Count 226 350 439 227 364 497 688 514 490 761 623 709 423 602 529 658 385 340 219 343 420 192 376 451


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.3 4.7 4.7 4.6 4.0 3.7 4.1 3.8 3.3 3.7 3.4 5.8 5.7 5.8 5.7 6.7 6.5 5.0 5.3 6.3
Std Deviation (1e-4) 0.5 0.8 1.1 1.4 1.4 1.3 0.9 1.3 1.0 1.2 1.3 1.1 0.8 1.1 1.2 1.4 1.6 1.4 0.8 1.1 1.7
Intensity Change (Log2) 0.0 -0.03 -0.2 -0.34 -0.2 -0.3 -0.51 -0.34 -0.47 0.31 0.3 0.31 0.3 0.52 0.48 0.1 0.19 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.4 3.3 4.3 -1.7 -1.1 0.3
Cytoplasm -0.7 -2.4 -5.9 -7.0 -8.2 -11.3 -10.3 -12.5 -13.1 1.8 1.7 2.6 -0.8 -1.2 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 5.1 5.4 4.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 0 0 0 0 0 0 0 0 0 3.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2018 0.4758 0.2371 0.0134 -0.1335 0.6337 2.4484 2.9962 2.9959 2.9248 2.9031 3.1697 4.7859 3.9376 4.2748 4.2167 3.7734 4.517
Actin 0.0236 0.0004 0.0118 0.0041 0.0126 0.0039 0.0102 0.0016 0.0061 0.0016 0.014 0.002 0.0553 0.002 0.0054 0.0004 0.0049 0.0029
Bud 0.0002 0.0001 0.0002 0.0002 0.0001 0.001 0.0004 0.001 0.0017 0.0002 0.0178 0.0007 0.001 0.0002 0.0031 0.0001 0.0286 0.0008
Bud Neck 0.0031 0.0007 0.0006 0.0005 0.0028 0.0032 0.0088 0.0007 0.0009 0.0003 0.0006 0.0056 0.0193 0.0002 0.0053 0.0002 0.0003 0.0007
Bud Periphery 0.0003 0.0002 0.0004 0.0004 0.0002 0.0088 0.0007 0.0011 0.0047 0.0004 0.0146 0.0007 0.0021 0.0003 0.006 0.0003 0.0104 0.0006
Bud Site 0.0012 0.0047 0.0055 0.0003 0.0007 0.0002 0.0035 0.0241 0.003 0.0002 0.0032 0.0003 0.0043 0.0026 0.0122 0.0001 0.0009 0.0002
Cell Periphery 0.0024 0.0028 0.0015 0.0004 0.0008 0.0009 0.0017 0.0016 0.0026 0.0006 0.0005 0.0004 0.001 0.0013 0.0022 0.0008 0.0002 0.0003
Cytoplasm 0.2704 0.3958 0.3343 0.2823 0.3209 0.3937 0.3216 0.3447 0.3626 0.2648 0.2692 0.3845 0.2793 0.5276 0.3732 0.1855 0.3417 0.3851
Cytoplasmic Foci 0.0262 0.0034 0.0069 0.0255 0.0139 0.0038 0.0056 0.0045 0.0087 0.0054 0.0179 0.0078 0.0284 0.0053 0.0096 0.0032 0.0037 0.0046
Eisosomes 0.0005 0.0004 0.0004 0.0003 0.0006 0.0002 0.0052 0.0002 0.0004 0.0004 0.0003 0.0001 0.0011 0.0002 0.0006 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0055 0.0048 0.0056 0.0051 0.0025 0.0037 0.0047 0.0021 0.0052 0.0022 0.0041 0.0021 0.0092 0.0047 0.0047 0.0032 0.0021 0.0029
Endosome 0.0069 0.0008 0.003 0.0029 0.0048 0.0007 0.0046 0.0006 0.0017 0.0014 0.0062 0.0031 0.0333 0.0011 0.0015 0.0007 0.0022 0.0023
Golgi 0.0026 0.0002 0.0009 0.0008 0.0022 0.0002 0.002 0.0002 0.0005 0.0003 0.0028 0.0007 0.0077 0.0003 0.0005 0.0001 0.0009 0.0003
Lipid Particles 0.0045 0.0008 0.0024 0.0018 0.0055 0.0007 0.0042 0.0004 0.0008 0.0007 0.009 0.0006 0.0118 0.0005 0.0009 0.0002 0.004 0.0006
Mitochondria 0.0006 0.0001 0.0003 0.0005 0.0005 0.0002 0.0018 0.0002 0.0005 0.0003 0.0009 0.0009 0.0035 0.0002 0.0004 0.0001 0.0013 0.0003
None 0.6332 0.5821 0.6195 0.6657 0.6215 0.5769 0.6177 0.612 0.595 0.7188 0.5892 0.5866 0.4889 0.45 0.5618 0.8022 0.5416 0.5875
Nuclear Periphery 0.0022 0.0004 0.0009 0.0008 0.0012 0.0003 0.002 0.0004 0.0013 0.0004 0.0033 0.0004 0.0124 0.0005 0.0019 0.0005 0.0228 0.0024
Nucleolus 0.0007 0.0002 0.0005 0.0002 0.0007 0.0001 0.0007 0.0004 0.0003 0.0001 0.0004 0.0002 0.0033 0.0002 0.0006 0.0001 0.0022 0.0003
Nucleus 0.0017 0.0009 0.0017 0.0008 0.0007 0.0005 0.0009 0.0013 0.0013 0.0004 0.0018 0.0009 0.0071 0.0011 0.0013 0.0007 0.0047 0.0028
Peroxisomes 0.0036 0.0001 0.0002 0.0034 0.0027 0.0001 0.0007 0.0002 0.0003 0.0003 0.028 0.0003 0.0074 0.0003 0.0006 0.0001 0.001 0.0005
Punctate Nuclear 0.0095 0.0004 0.002 0.0033 0.0042 0.0004 0.0014 0.0016 0.0013 0.0005 0.0152 0.0012 0.0165 0.0006 0.0072 0.0005 0.025 0.004
Vacuole 0.001 0.001 0.0013 0.0007 0.0005 0.0006 0.0013 0.0008 0.0011 0.0006 0.0008 0.0009 0.0053 0.0008 0.0009 0.0007 0.0006 0.0005
Vacuole Periphery 0.0002 0 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0002 0.0003 0.0016 0.0001 0.0001 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.8977 3.1676 3.7607 8.245 4.5941 6.3745 8.4872 7.998 9.9294 8.5903
Translational Efficiency 1.2458 1.211 0.9685 0.5873 0.611 0.7923 0.5914 0.7836 0.6378 0.6403

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
Localization
Cell Percentages cytoplasm (40%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Art10

Art10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Art10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available