Standard name
Human Ortholog
Description ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.06 0.05 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0.07 0.09 0.11 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0.64 0.17 0.67 0.64 0.69 0.3 0.39 0.27 0.15 0.07 0.12 0.16 0.23 0.21 0.25 0.62 0.68 0.71 0.82 0.74 0.87 0.83 0.84 0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.57 0.24 0.31 0.3 0.55 0.48 0.48 0.76 0.67 0.72 0.66 0 0 0 0.18 0.11 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.2 0.35 0.23 0.2 0.14 0.11 0.08 0.13 0.1 0.1 0.09 0.14 0.11 0.13 0.07 0.08 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.07 0.05 0.07 0.16 0.15 0 0.12 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.18 0.05 0.24 0.18 0.16 0.17 0.23 0.26 0.12 0.21 0.17 0.2 0.47 0.45 0.43 0.27 0.18 0.14 0 0 0 0.05 0 0
Vac/Vac Membrane 0.25 0.36 0.13 0.09 0.08 0.06 0.05 0.06 0 0 0 0.05 0.34 0.29 0.33 0.19 0.26 0.27 0 0.12 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 1 2 3 4 14 3 0 0 0 1 0 0 0 1 1
Bud 0 2 2 3 3 8 29 21 18 31 26 33 0 1 0 2 3 0 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 3 6 1 3 5 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 3 5 11 9 11 14 18 7 18 7 10 1 13 16 25 21 19 0 0 0 0 0 0
Endoplasmic Reticulum 0 2 1 0 0 0 1 0 2 2 0 2 1 1 2 10 9 11 1 0 0 0 0 1
Endosome 128 37 198 138 221 89 207 94 56 33 40 71 23 40 47 143 142 119 170 183 280 220 323 324
Golgi 1 0 0 1 1 0 1 0 0 0 0 0 1 2 2 0 5 1 4 4 3 1 3 6
Mitochondria 51 123 71 68 98 166 252 169 276 295 235 294 0 1 0 42 23 17 5 7 1 6 7 9
Nucleus 1 5 2 0 0 0 3 2 3 3 1 0 2 4 5 3 0 0 1 3 0 1 1 0
Nuclear Periphery 6 8 5 4 4 2 2 1 2 5 2 3 3 4 0 5 8 4 2 2 1 2 7 8
Nucleolus 65 44 103 50 64 41 59 28 49 43 34 39 14 21 24 17 17 15 1 2 1 5 5 2
Peroxisomes 1 0 16 15 16 21 82 52 16 54 28 41 0 0 0 2 3 0 2 1 5 1 1 4
SpindlePole 36 11 70 39 52 52 121 93 43 92 54 90 48 85 81 61 37 24 8 8 12 13 10 8
Vac/Vac Membrane 50 78 37 19 26 19 24 21 16 6 8 20 35 56 62 43 54 45 9 30 9 10 19 31
Unique Cell Count 199 216 295 216 322 300 528 353 365 441 326 444 102 191 188 229 210 168 208 247 321 267 386 405
Labelled Cell Count 345 313 510 348 494 410 800 506 490 586 442 612 132 242 242 353 323 255 208 247 321 267 386 405


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.6 6.0 5.9 5.4 4.8 4.8 5.0 4.7 4.3 4.6 4.7 9.5 9.7 10.2 5.0 6.0 7.3 8.6 9.0 9.4
Std Deviation (1e-4) 0.9 1.0 1.0 1.3 1.1 1.1 1.1 1.3 0.8 1.0 0.8 0.9 0.8 1.1 1.1 0.8 1.4 1.2 1.2 1.3 1.6
Intensity Change (Log2) – – – -0.03 -0.16 -0.32 -0.32 -0.27 -0.37 -0.51 -0.39 -0.37 0.66 0.68 0.75 -0.28 -0.0 0.27 0.52 0.58 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 4.7 0 0 0 0
Bud 0 0 0 0 3.6 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 0.9 1.5 0.9 2.3 0.2 1.8 0.4 0.5 0 2.9 3.6 4.5 4.2 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.8 0.4 0 0 -10.3 0 0 0 0 -7.8 -9.9 -9.0 -1.1 0.1 0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 1.8 0 0 6.2 0 0 0 0 -5.5 -7.1 -7.3 -1.6 -3.7 -3.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1.5 0
Nucleolus -2.9 -4.2 0 0 -8.5 0 0 0 0 -4.0 -5.9 -5.4 -7.4 -7.0 -6.2
Peroxisomes 0.7 -0.3 0 0 3.8 0 0 0 0 0 -3.3 -3.2 -2.8 -2.3 -3.1
SpindlePole -1.5 -2.4 0 0 0.8 0 0 0 0 4.4 4.8 4.5 0.8 -1.7 -2.4
Vacuole -1.3 -1.8 -2.6 -4.2 -2.9 -3.8 -6.3 -4.8 -4.0 4.9 4.6 5.4 2.0 3.8 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8644 3.4072 3.0526 3.0097 2.7398 2.9 3.5191 3.7211 3.275 3.0928 2.886 3.2342 3.1842 4.1122 3.7498 3.8933 2.7724 3.6426
Actin 0.0067 0.0001 0.0102 0 0.0002 0 0.002 0.0001 0.0027 0 0 0.0001 0.0071 0.0003 0.0002 0 0.0001 0.0001
Bud 0.0001 0.0002 0.0001 0 0 0 0.0003 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0
Bud Neck 0.0005 0.0007 0.0006 0.0001 0.0003 0.0006 0.0023 0.0002 0.0002 0.0001 0.0002 0.0002 0.0004 0.0007 0.0003 0.0001 0.0005 0.0003
Bud Periphery 0.0002 0.0002 0.0002 0 0.0001 0 0.0004 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0001
Bud Site 0.0004 0.0009 0.0008 0.0001 0.0001 0.0003 0.0045 0.0006 0.0003 0.0001 0.0001 0.0001 0.0003 0.0004 0.0003 0 0.0004 0.0002
Cell Periphery 0.0002 0.0003 0.0002 0 0.0001 0.0001 0.0004 0.0002 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0002
Cytoplasm 0.0033 0.0023 0.0012 0.0009 0.0007 0.0007 0.0024 0.0045 0.0009 0.0005 0.0004 0.0003 0.0009 0.0014 0.0006 0.0004 0.0006 0.0004
Cytoplasmic Foci 0.1015 0.1333 0.1166 0.1593 0.0837 0.1073 0.1435 0.1599 0.1683 0.1545 0.1747 0.1167 0.1251 0.18 0.1487 0.1956 0.1303 0.1071
Eisosomes 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0067 0.0005 0.0004 0.0001 0.0007 0.0007 0.0004 0.0006 0.0004 0.0001 0.0001 0.0001 0.0006 0.0005 0.0002 0 0.0003 0.0002
Endosome 0.524 0.4996 0.514 0.5573 0.6406 0.4981 0.4309 0.3548 0.3719 0.5763 0.5215 0.3248 0.4465 0.3675 0.4072 0.58 0.4998 0.3542
Golgi 0.0562 0.0545 0.0799 0.1134 0.0699 0.0926 0.0582 0.0544 0.0855 0.0777 0.0568 0.087 0.0621 0.0486 0.0876 0.085 0.0779 0.0774
Lipid Particles 0.1707 0.1883 0.1613 0.0961 0.0895 0.2209 0.2525 0.3335 0.2862 0.1469 0.1589 0.3919 0.2805 0.2994 0.2711 0.113 0.2018 0.3849
Mitochondria 0.0037 0.0026 0.0097 0.0013 0.0083 0.0024 0.0102 0.0017 0.0037 0.0007 0.0007 0.0021 0.0024 0.0061 0.0072 0.0005 0.0018 0.0028
None 0.002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0023 0.0015 0.0009 0 0 0.0001 0.0013 0.0002 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0141 0.0024 0.0005 0.0001 0.033 0.0022 0.0007 0.0005 0.0005 0.0002 0.0001 0.0003 0.0017 0.0012 0.0003 0 0.0021 0.0005
Nucleolus 0.001 0.0053 0.0086 0.0001 0.0012 0.0025 0.0026 0.0017 0.0005 0.0001 0.0003 0.0008 0.001 0.0019 0.0009 0.0002 0.0081 0.0016
Nucleus 0.0027 0.0008 0.0016 0 0.0027 0.0006 0.0012 0.0004 0.0001 0 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0032 0.0003
Peroxisomes 0.0269 0.0222 0.0424 0.0426 0.0187 0.0258 0.0255 0.0429 0.0594 0.0319 0.0517 0.0577 0.0362 0.0493 0.0551 0.0196 0.0503 0.0444
Punctate Nuclear 0.0027 0.0024 0.0075 0.0005 0.0014 0.002 0.0067 0.0192 0.0043 0.0004 0.0004 0.012 0.0022 0.0117 0.0022 0.0004 0.0038 0.0016
Vacuole 0.0562 0.0639 0.036 0.0188 0.0366 0.0312 0.0409 0.0193 0.0081 0.0067 0.0265 0.0035 0.0235 0.0237 0.0132 0.0032 0.0129 0.0172
Vacuole Periphery 0.0201 0.0196 0.0081 0.009 0.0119 0.0119 0.012 0.0039 0.0058 0.0039 0.0075 0.0024 0.0076 0.0069 0.0044 0.0017 0.0057 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.8959 10.9281 9.5194 13.7284 15.8497 12.7491 13.2112 12.1591 12.5246 15.5416
Translational Efficiency 0.5946 0.6434 0.853 0.5371 0.457 0.4687 0.695 0.5343 0.6449 0.5464

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains
Localization
Cell Percentages endosome (64%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps33

Vps33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available