Standard name | |
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Human Ortholog | |
Description | ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 1 | 1 | – | 2 | 5 | 3 | 6 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 2 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | 6 | – | 10 | 10 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 1 | 5 | 12 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 20 | 12 | 7 | 8 | 17 | 8 | – | 12 | 21 | 14 | 16 | 2 | 2 | 5 | 14 | 15 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 130 | 564 | 382 | 281 | 327 | 390 | 450 | – | 380 | 374 | 225 | 241 | 250 | 299 | 345 | 318 | 250 | 251 | 129 | 599 | 368 | 145 | 174 | 218 |
Endoplasmic Reticulum | 2 | 4 | 7 | 12 | 4 | 9 | 5 | – | 10 | 10 | 6 | 33 | 3 | 3 | 0 | 11 | 13 | 9 | 7 | 15 | 10 | 40 | 37 | 45 |
Endosome | 0 | 9 | 1 | 0 | 2 | 7 | 2 | – | 4 | 5 | 1 | 0 | 0 | 2 | 3 | 3 | 4 | 0 | 0 | 3 | 1 | 3 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 2 | 8 | 0 | 6 | 29 | 66 | 137 | – | 260 | 234 | 229 | 101 | 3 | 1 | 3 | 7 | 2 | 4 | 0 | 3 | 17 | 18 | 13 | 22 |
Nucleus | 3 | 2 | 2 | 1 | 1 | 0 | 2 | – | 0 | 3 | 1 | 1 | 0 | 0 | 2 | 5 | 4 | 1 | 0 | 4 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 39 | 9 | 5 | 18 | 15 | 7 | – | 12 | 31 | 18 | 10 | 0 | 1 | 1 | 49 | 36 | 12 | 1 | 21 | 10 | 23 | 23 | 31 |
Unique Cell Count | 135 | 609 | 400 | 301 | 359 | 463 | 550 | 557 | 567 | 406 | 367 | 258 | 306 | 351 | 370 | 290 | 265 | 144 | 670 | 429 | 240 | 274 | 354 | |
Labelled Cell Count | 140 | 648 | 415 | 313 | 389 | 507 | 621 | 692 | 698 | 503 | 415 | 264 | 309 | 360 | 412 | 326 | 283 | 144 | 670 | 429 | 240 | 274 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.9 | 8.0 | 7.5 | 7.1 | 5.8 | 5.7 | – | 5.1 | 5.2 | 4.8 | 5.6 | 7.0 | 7.0 | 7.0 | 10.9 | 11.7 | 10.4 | 6.9 | 7.0 | 7.3 |
Std Deviation (1e-4) | 1.7 | 1.7 | 1.4 | 1.4 | 1.3 | 1.2 | 1.1 | – | 0.9 | 1.0 | 1.0 | 1.0 | 1.4 | 1.5 | 1.5 | 2.0 | 1.7 | 1.8 | 1.5 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.17 | -0.45 | -0.48 | – | -0.63 | -0.61 | -0.74 | -0.52 | -0.18 | -0.19 | -0.19 | 0.46 | 0.55 | 0.38 | -0.22 | -0.19 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -0.7 | 0.5 | -1.6 | – | -0.8 | 0.6 | 0 | 1.0 | -1.9 | -2.2 | -1.4 | 0.6 | 1.5 | -0.9 |
Cytoplasm | -1.2 | -2.4 | -5.4 | -6.3 | – | -10.3 | -11.0 | -13.2 | -10.6 | 0.9 | 1.6 | 2.2 | -4.6 | -4.3 | -0.5 |
Endoplasmic Reticulum | 1.8 | -0.7 | 0.2 | -1.1 | – | 0.1 | 0 | 0 | 4.5 | 0 | 0 | 0 | 1.1 | 2.1 | 1.4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.8 | 7.9 | 10.8 | – | 16.0 | 14.8 | 0 | 11.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 2.1 | 0.9 | -1.2 | – | -0.1 | 2.5 | 1.7 | 0.4 | 0 | 0 | 0 | 5.8 | 5.3 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8405 | 4.382 | 3.182 | 3.2539 | 3.0138 | 3.7367 | 4.3358 | 5.3682 | 4.6436 | 3.8821 | 4.5955 | 4.5952 | 4.0438 | 4.9041 | 4.5062 | 4.1441 | 4.3184 | 4.587 |
Actin | 0.0015 | 0.0028 | 0.0079 | 0.0011 | 0.0051 | 0.0023 | 0.0319 | 0.0029 | 0.0178 | 0.0024 | 0.0284 | 0.0058 | 0.0158 | 0.0006 | 0.0133 | 0.0004 | 0.0067 | 0.0071 |
Bud | 0.0016 | 0.0019 | 0.0023 | 0.001 | 0.0006 | 0.0015 | 0.0017 | 0.0045 | 0.0035 | 0.0012 | 0.0092 | 0.0008 | 0.0067 | 0.002 | 0.0057 | 0.0006 | 0.0041 | 0.0036 |
Bud Neck | 0.0008 | 0.0005 | 0.0008 | 0.0013 | 0.0012 | 0.0033 | 0.0045 | 0.0004 | 0.0012 | 0.0017 | 0.0031 | 0.0022 | 0.0047 | 0.0003 | 0.0011 | 0.0011 | 0.0022 | 0.0013 |
Bud Periphery | 0.0008 | 0.0019 | 0.0035 | 0.0005 | 0.0006 | 0.002 | 0.0016 | 0.0052 | 0.0041 | 0.0008 | 0.0087 | 0.0007 | 0.002 | 0.0055 | 0.002 | 0.0003 | 0.0046 | 0.0016 |
Bud Site | 0.0027 | 0.0109 | 0.0025 | 0.0004 | 0.0007 | 0.0017 | 0.0084 | 0.0072 | 0.0101 | 0.0007 | 0.0479 | 0.0012 | 0.0065 | 0.0024 | 0.0059 | 0.0012 | 0.0275 | 0.0002 |
Cell Periphery | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0002 |
Cytoplasm | 0.7054 | 0.823 | 0.7218 | 0.6696 | 0.7014 | 0.7399 | 0.5261 | 0.861 | 0.7454 | 0.7655 | 0.5263 | 0.7979 | 0.6102 | 0.9005 | 0.7878 | 0.7477 | 0.6646 | 0.8205 |
Cytoplasmic Foci | 0.0384 | 0.0292 | 0.0403 | 0.0551 | 0.0373 | 0.0505 | 0.0549 | 0.0227 | 0.0302 | 0.0304 | 0.0641 | 0.0305 | 0.0612 | 0.0192 | 0.0268 | 0.0479 | 0.0449 | 0.0332 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0273 | 0.0103 | 0.0118 | 0.0184 | 0.0221 | 0.0201 | 0.0302 | 0.0139 | 0.015 | 0.0147 | 0.0068 | 0.0211 | 0.0288 | 0.0115 | 0.0169 | 0.009 | 0.0129 | 0.0142 |
Endosome | 0.0934 | 0.0618 | 0.1144 | 0.1313 | 0.1061 | 0.1179 | 0.1499 | 0.0404 | 0.0778 | 0.0854 | 0.1231 | 0.0843 | 0.1208 | 0.0318 | 0.0748 | 0.1341 | 0.0756 | 0.0672 |
Golgi | 0.0102 | 0.0129 | 0.0064 | 0.0147 | 0.0094 | 0.0114 | 0.0319 | 0.0058 | 0.0196 | 0.0106 | 0.0531 | 0.0113 | 0.0152 | 0.0031 | 0.0054 | 0.0045 | 0.0107 | 0.0067 |
Lipid Particles | 0.0023 | 0.0002 | 0.0007 | 0.0005 | 0.0118 | 0.0004 | 0.0152 | 0.0004 | 0.0081 | 0.0009 | 0.0118 | 0.002 | 0.0094 | 0.0001 | 0.0027 | 0.0007 | 0.0042 | 0.0014 |
Mitochondria | 0.0099 | 0.0144 | 0.0073 | 0.0051 | 0.006 | 0.009 | 0.0242 | 0.0019 | 0.0197 | 0.0023 | 0.0656 | 0.0078 | 0.0069 | 0.0021 | 0.005 | 0.0021 | 0.0314 | 0.0053 |
None | 0.06 | 0.0132 | 0.0501 | 0.0718 | 0.0475 | 0.0228 | 0.0294 | 0.0166 | 0.0129 | 0.0467 | 0.0046 | 0.0127 | 0.0548 | 0.01 | 0.0212 | 0.0235 | 0.0877 | 0.0223 |
Nuclear Periphery | 0.0054 | 0.0011 | 0.0018 | 0.0028 | 0.0201 | 0.0019 | 0.021 | 0.0015 | 0.0027 | 0.0063 | 0.0059 | 0.0035 | 0.0087 | 0.001 | 0.0037 | 0.0022 | 0.002 | 0.0029 |
Nucleolus | 0.0018 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0038 | 0.0001 | 0.0002 | 0.0001 | 0.0017 | 0.0003 | 0.0007 | 0 | 0.0015 | 0.0001 | 0.0003 | 0.0001 |
Nucleus | 0.0083 | 0.0015 | 0.0023 | 0.0019 | 0.0043 | 0.0018 | 0.0257 | 0.0019 | 0.0018 | 0.0031 | 0.0058 | 0.0044 | 0.016 | 0.0013 | 0.0056 | 0.0021 | 0.002 | 0.0016 |
Peroxisomes | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0011 | 0.0007 | 0.0059 | 0.0016 | 0.0101 | 0.0004 | 0.01 | 0.002 | 0.0084 | 0.0001 | 0.0014 | 0.0004 | 0.0081 | 0.0035 |
Punctate Nuclear | 0.0051 | 0.0002 | 0.0008 | 0.0004 | 0.005 | 0.0011 | 0.0129 | 0.0005 | 0.0009 | 0.0006 | 0.0036 | 0.0016 | 0.0032 | 0.0002 | 0.0036 | 0.0009 | 0.0019 | 0.0007 |
Vacuole | 0.0207 | 0.0106 | 0.0197 | 0.0178 | 0.0133 | 0.0084 | 0.015 | 0.0097 | 0.0153 | 0.0219 | 0.0133 | 0.007 | 0.0165 | 0.0064 | 0.0121 | 0.0171 | 0.0064 | 0.0047 |
Vacuole Periphery | 0.0031 | 0.0026 | 0.0043 | 0.0055 | 0.005 | 0.003 | 0.0049 | 0.0015 | 0.003 | 0.0042 | 0.0043 | 0.0027 | 0.0031 | 0.0014 | 0.0026 | 0.0038 | 0.0019 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.1981 | 24.8383 | 30.6144 | 23.2702 | 24.1183 | 12.2376 | 23.6242 | 24.5635 | 21.145 | 25.1125 |
Translational Efficiency | 1.1944 | 1.1935 | 0.9273 | 1.0556 | 1.0724 | 1.8076 | 1.1826 | 0.8048 | 0.8409 | 0.9072 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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