Standard name
Human Ortholog
Description Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0.08 0.1 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.75 0.68 0.68 0.74 0.75 0.62 0.58 0.56 0.52 0.51 0.47 0.46 0.74 0.75 0.67 – – – 0.58 0.62 0.57 0.56 0.47 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.69 0.56 0.53 0.52 0.38 0.62 0.64 0.65 0.69 0.76 0.8 0.71 0.65 0.4 0.46 – – – 0.39 0.31 0.4 0.38 0.41 0.45
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 1 1
Bud Neck 0 0 0 0 1 0 6 3 0 0 3 1 1 0 0 – – – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 2 5 4 2 3 0 2 2 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 2 0 7 13 5 12 9 15 10 8 3 6 2 18 26 – – – 0 0 0 1 1 8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 – – – 0 0 0 2 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 8 38 2 1 1 10 5 2 10 6 6 5 1 1 0 – – – 0 2 0 0 3 5
Nucleus 149 290 121 112 114 146 194 213 117 117 91 71 157 164 180 – – – 117 293 119 90 128 110
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 136 240 94 79 58 146 216 247 155 174 156 110 137 89 122 – – – 78 147 83 62 112 130
Peroxisomes 0 4 0 0 0 0 2 1 1 0 0 0 0 1 2 – – – 0 0 0 0 0 0
SpindlePole 0 3 1 0 1 0 2 2 0 0 1 2 0 4 7 – – – 0 3 0 1 0 2
Vac/Vac Membrane 0 3 0 0 2 2 4 3 4 1 1 1 0 2 1 – – – 5 20 2 1 14 19
Unique Cell Count 198 425 177 152 151 234 337 380 225 228 194 156 212 220 268 203 472 210 162 272 289
Labelled Cell Count 295 578 225 206 182 316 440 489 303 310 263 199 298 283 340 203 472 210 162 272 289


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 28.2 22.5 19.7 21.3 16.9 16.8 16.8 16.6 15.6 14.9 15.3 25.2 25.2 23.1 – – – 21.5 21.5 21.6
Std Deviation (1e-4) 6.1 11.1 10.2 8.0 9.2 9.4 8.0 6.5 9.5 7.6 7.8 7.2 8.3 11.4 10.5 – – – 9.2 7.7 9.5
Intensity Change (Log2) – – – -0.2 -0.08 -0.41 -0.42 -0.43 -0.44 -0.53 -0.59 -0.56 0.16 0.16 0.04 – – – -0.07 -0.07 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 1.7 -0.3 0.6 -0.8 0 0.2 -0.2 0 -0.1 0 1.7 2.3 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 1.9 0 0 1.9 0 0 0 0 0 0 – – –
Nucleus 1.1 1.4 -1.3 -2.4 -2.8 -3.3 -3.5 -4.2 -4.2 1.2 1.4 -0.3 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus -0.2 -2.7 1.9 2.4 2.7 3.2 4.9 5.6 3.3 2.3 -2.5 -1.6 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.0586 17.2108 15.7798 14.2613 17.615 15.9312 19.7116 19.3667 17.3372 16.9634 16.8891 18.4948 22.4512 24.6389 21.1851 22.7927 19.2235 22.9081
Actin 0 0.0001 0.0001 0.0005 0 0 0.0001 0 0.0008 0.0074 0 0 0.0095 0.002 0.0056 0.0018 0.0102 0
Bud 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0.0001 0 0.0005 0.0001 0.0002 0 0.0004 0
Bud Neck 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0004 0.0001
Bud Periphery 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0005 0.0002 0 0.0004 0.0001 0.0002 0 0.0007 0
Bud Site 0 0.0004 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0.0004 0.0001 0 0.0008 0.0003 0.0023 0.0001 0.002 0
Cell Periphery 0 0 0.0001 0 0.0001 0.0002 0 0 0 0.0002 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001
Cytoplasm 0 0 0.0003 0.0002 0 0 0.0004 0 0.0016 0.0003 0 0.0002 0.0081 0.0001 0.0015 0.0002 0.0012 0
Cytoplasmic Foci 0 0.0002 0.0019 0.0003 0 0 0.0055 0 0.0023 0.0031 0 0.0003 0.0105 0.0003 0.0045 0.0036 0.0087 0.0025
Eisosomes 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0001 0 0.0008 0.0002 0 0 0.0022 0 0.0006 0.0007 0.0013 0
Endosome 0 0 0.0001 0.0001 0 0 0.0015 0 0.014 0.0023 0 0 0.0077 0.0002 0.0081 0.0035 0.0207 0
Golgi 0 0 0.0001 0.0001 0 0 0.0005 0 0.0021 0.0016 0 0 0.0016 0.0002 0.0017 0.0014 0.0187 0
Lipid Particles 0 0.0001 0 0.0001 0 0 0.0027 0 0.0042 0.0026 0.0001 0.0001 0.0064 0.0001 0.0052 0.0071 0.0078 0.0002
Mitochondria 0.0005 0.0007 0.001 0.0009 0.0013 0.0004 0.0009 0.0003 0.0011 0.0073 0.0064 0.0001 0.0067 0.0009 0.0052 0.0036 0.0111 0.0004
None 0 0 0.0002 0.0001 0 0 0.0004 0 0.0003 0.0005 0 0.0001 0.0092 0.0001 0.0009 0.0006 0.001 0.0001
Nuclear Periphery 0 0.0002 0.0007 0.0077 0.0001 0 0.0018 0.0001 0.0057 0.0033 0.0001 0.0003 0.0069 0.0011 0.0042 0.0071 0.0213 0
Nucleolus 0.1633 0.2075 0.0989 0.1411 0.4942 0.439 0.176 0.0704 0.0725 0.1333 0.4631 0.2921 0.2011 0.1054 0.0906 0.1071 0.4859 0.2989
Nucleus 0.8359 0.7811 0.7807 0.7437 0.5036 0.5585 0.7836 0.929 0.8861 0.8285 0.5277 0.7028 0.7052 0.8838 0.8638 0.8601 0.3882 0.6972
Peroxisomes 0 0.0001 0.0001 0 0 0 0.0009 0 0.0014 0.0026 0.0001 0 0.0043 0.0002 0.0008 0.0006 0.0037 0
Punctate Nuclear 0 0.0091 0.1154 0.1046 0.0003 0 0.0247 0.0001 0.0051 0.0043 0.0001 0.0035 0.0156 0.0048 0.0027 0.0011 0.0126 0.0002
Vacuole 0 0.0001 0.0001 0.0001 0.0001 0.0007 0.0002 0 0.0009 0.0004 0.0005 0.0001 0.0013 0.0002 0.0008 0.0004 0.0019 0.0001
Vacuole Periphery 0.0001 0.0002 0.0002 0.0002 0.0001 0.0007 0.0002 0 0.001 0.0008 0.0011 0 0.0015 0.0001 0.0009 0.0008 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.9656 38.7659 46.1676 57.9107 58.6544 36.3852 50.9308 62.5443 68.1082 66.006
Translational Efficiency 1.2057 1.2319 1.1432 1.4164 1.1313 1.2168 1.078 1.1332 1.0619 1.0222

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Bdf1

Bdf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bdf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available