Standard name | |
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Human Ortholog | |
Description | Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 0 | 1 | 0 | 1 | 0 | – | 2 | 2 | 3 | 1 | – | 0 | 0 | 0 | 1 | 0 | 3 | 5 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 1 | 5 | – | 2 | 7 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 16 | 10 | 12 | 109 | 186 | 218 | – | 102 | 135 | 94 | 134 | – | 5 | 50 | 13 | 13 | 16 | 2 | 6 | 2 | 2 | 1 | 2 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 5 | 5 | 3 | 1 | 0 | 2 | 0 | 0 | 0 |
Endosome | 1 | 1 | 0 | 2 | 1 | 0 | 2 | – | 0 | 1 | 1 | 0 | – | 0 | 3 | 18 | 25 | 25 | 2 | 2 | 2 | 2 | 2 | 3 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 4 | 4 | 2 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 12 | 6 | 0 | 6 | 3 | 25 | 38 | – | 78 | 65 | 170 | 84 | – | 0 | 1 | 9 | 4 | 6 | 3 | 3 | 0 | 0 | 0 | 0 |
Nucleus | 241 | 244 | 114 | 133 | 28 | 5 | 27 | – | 16 | 28 | 22 | 20 | – | 8 | 9 | 98 | 129 | 93 | 262 | 248 | 115 | 29 | 48 | 30 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 1 | 2 | 1 | 0 | 0 | – | 0 | 0 | 1 | 1 | – | 1 | 0 | 24 | 29 | 28 | 0 | 3 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 2 | 3 | 2 | 4 | – | 3 | 2 | 4 | 2 | – | 1 | 1 | 15 | 24 | 19 | 3 | 8 | 3 | 1 | 2 | 3 |
Vac/Vac Membrane | 22 | 44 | 15 | 7 | 26 | 17 | 13 | – | 3 | 7 | 6 | 7 | – | 1 | 14 | 32 | 19 | 36 | 22 | 26 | 20 | 3 | 4 | 3 |
Unique Cell Count | 273 | 289 | 140 | 155 | 160 | 222 | 278 | 177 | 212 | 238 | 228 | 16 | 73 | 169 | 206 | 181 | 306 | 311 | 155 | 43 | 64 | 52 | ||
Labelled Cell Count | 285 | 314 | 144 | 166 | 174 | 238 | 308 | 207 | 250 | 303 | 255 | 17 | 79 | 219 | 256 | 232 | 306 | 311 | 155 | 43 | 64 | 52 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 6.4 | 7.0 | 5.9 | 5.5 | 4.3 | 4.2 | – | 3.6 | 3.9 | 3.4 | 3.7 | – | 6.1 | 5.6 | 9.9 | 9.9 | 9.2 | – | 7.2 | – |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.5 | 1.1 | 1.4 | 1.5 | 1.2 | – | 0.8 | 1.0 | 0.9 | 1.1 | – | 1.8 | 1.4 | 2.7 | 2.4 | 2.4 | – | 1.6 | – |
Intensity Change (Log2) | – | – | – | -0.24 | -0.34 | -0.69 | -0.72 | – | -0.97 | -0.83 | -1.03 | -0.9 | – | -0.2 | -0.32 | 0.51 | 0.5 | 0.41 | – | 0.05 | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 10.8 | 14.3 | 13.8 | – | 9.3 | 10.5 | 6.8 | 9.9 | – | 0 | 9.4 | 0.2 | -0.3 | 0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.0 | 4.3 | 4.6 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.0 | -11.1 | -15.6 | -14.6 | – | -13.0 | -12.8 | -14.1 | -14.1 | – | -2.9 | -9.7 | -4.4 | -3.8 | -5.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.3 | 4.3 | 4.6 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.9 | 3.5 | 3.3 |
Vacuole | -2.0 | 1.4 | -1.0 | -2.3 | – | -3.4 | -2.8 | -3.4 | -3.0 | – | 0 | 1.7 | 2.0 | -0.5 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7726 | 1.5538 | 0.9519 | 1.0915 | 0.6866 | 1.0784 | 1.0945 | 1.1467 | 1.0974 | 1.0102 | 1.0553 | 1.1514 | 0.5108 | 0.9399 | 0.6913 | 1.0124 | 1.0045 | 0.6855 |
Actin | 0.0096 | 0.0001 | 0.0045 | 0.0001 | 0.0239 | 0.0032 | 0.0118 | 0.0001 | 0.0019 | 0.0033 | 0.0055 | 0.0004 | 0.0089 | 0.0001 | 0.0113 | 0.0027 | 0.0345 | 0.0008 |
Bud | 0.0003 | 0.0026 | 0 | 0 | 0.0015 | 0.0008 | 0.0005 | 0 | 0.0005 | 0.0002 | 0.0025 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0011 | 0.0001 | 0.0001 | 0.0005 | 0.0078 | 0.0031 | 0.0088 | 0.0002 | 0.0034 | 0.003 | 0.0015 | 0.004 | 0.0005 | 0.0003 | 0.0008 | 0.0006 | 0.0011 | 0.0011 |
Bud Periphery | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0028 | 0.0003 | 0.0016 | 0 | 0.001 | 0.0004 | 0.0087 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0013 | 0.0047 | 0.0001 | 0.0002 | 0.0153 | 0.0002 | 0.0035 | 0.0001 | 0.0024 | 0.0009 | 0.0044 | 0 | 0.001 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0053 | 0.0058 | 0.0025 | 0.0101 | 0.0207 | 0.0037 | 0.0129 | 0.0094 | 0.0063 | 0.006 | 0.0195 | 0.0043 | 0.0025 | 0.019 | 0.0141 | 0.056 | 0.0569 | 0.0079 |
Cytoplasmic Foci | 0.0086 | 0.0003 | 0.0005 | 0.0057 | 0.0099 | 0.0027 | 0.0093 | 0.0003 | 0.0017 | 0.0047 | 0.0128 | 0.0009 | 0.0038 | 0.0001 | 0.0003 | 0.0004 | 0.0028 | 0.0002 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0.0008 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0016 | 0.0002 | 0.0001 | 0.001 | 0.0038 | 0.0008 | 0.0022 | 0.0005 | 0.002 | 0.0007 | 0.0035 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0022 | 0.0038 | 0.0007 |
Endosome | 0.0132 | 0.0005 | 0.001 | 0.0094 | 0.0264 | 0.0033 | 0.0092 | 0.0008 | 0.0031 | 0.0042 | 0.0269 | 0.0006 | 0.0108 | 0.0002 | 0.0008 | 0.0083 | 0.0154 | 0.0038 |
Golgi | 0.0025 | 0 | 0.0017 | 0.0002 | 0.0116 | 0.0015 | 0.0036 | 0 | 0.0005 | 0.0016 | 0.0051 | 0.0001 | 0.0034 | 0 | 0.0005 | 0.0005 | 0.0041 | 0.0003 |
Lipid Particles | 0.0092 | 0 | 0.0003 | 0.003 | 0.0182 | 0.0039 | 0.0145 | 0.0001 | 0.0027 | 0.0146 | 0.0107 | 0.0005 | 0.0027 | 0 | 0.0002 | 0.0004 | 0.0061 | 0.0003 |
Mitochondria | 0.0067 | 0.0003 | 0.0003 | 0.0006 | 0.0506 | 0.0045 | 0.0052 | 0.0003 | 0.0007 | 0.0086 | 0.0174 | 0.0001 | 0.0013 | 0.0002 | 0.0005 | 0.0015 | 0.0018 | 0.0011 |
None | 0.0212 | 0.0028 | 0.0194 | 0.0061 | 0.0805 | 0.0114 | 0.0196 | 0.0226 | 0.0209 | 0.0213 | 0.0374 | 0.0036 | 0.0156 | 0.0093 | 0.027 | 0.0186 | 0.0059 | 0.0014 |
Nuclear Periphery | 0.0137 | 0.0148 | 0.0064 | 0.0095 | 0.0306 | 0.0058 | 0.02 | 0.0165 | 0.0098 | 0.0184 | 0.0339 | 0.0038 | 0.0115 | 0.005 | 0.014 | 0.0359 | 0.0342 | 0.0133 |
Nucleolus | 0.0131 | 0.005 | 0.0065 | 0.0076 | 0.0123 | 0.0187 | 0.0131 | 0.007 | 0.0095 | 0.016 | 0.0108 | 0.0116 | 0.005 | 0.0033 | 0.0043 | 0.0035 | 0.0059 | 0.0064 |
Nucleus | 0.8318 | 0.9515 | 0.9332 | 0.9017 | 0.5774 | 0.9101 | 0.8168 | 0.9276 | 0.916 | 0.8518 | 0.7528 | 0.9425 | 0.9061 | 0.9503 | 0.9074 | 0.8608 | 0.808 | 0.9555 |
Peroxisomes | 0.0037 | 0 | 0.0002 | 0.0002 | 0.0048 | 0.0007 | 0.0155 | 0 | 0.0031 | 0.023 | 0.0116 | 0.0016 | 0.0035 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0001 |
Punctate Nuclear | 0.0541 | 0.0098 | 0.0226 | 0.0408 | 0.092 | 0.0246 | 0.0256 | 0.0139 | 0.0136 | 0.0178 | 0.0234 | 0.0257 | 0.0201 | 0.0107 | 0.0164 | 0.0059 | 0.0158 | 0.0056 |
Vacuole | 0.0013 | 0.0007 | 0.0002 | 0.0026 | 0.0048 | 0.0005 | 0.0038 | 0.0004 | 0.0005 | 0.0015 | 0.0077 | 0.0001 | 0.0011 | 0.0004 | 0.0004 | 0.0011 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.0013 | 0.0002 | 0.0001 | 0.0005 | 0.0035 | 0.0004 | 0.0018 | 0.0002 | 0.0002 | 0.0011 | 0.0031 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0011 | 0.0009 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.9629 | 31.9454 | 43.3982 | 34.8466 | 41.6995 | 25.8253 | 34.1634 | 30.6881 | 26.6107 | 33.0872 |
Translational Efficiency | 1.5703 | 1.6585 | 1.1307 | 1.074 | 0.9611 | 1.26 | 1.0836 | 0.9092 | 1.0028 | 0.978 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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