Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.96 0.98 1.0 0.98 0.98 0.93 0.85 0.9 0.75 0.82 0.67 0.81 1.0 0.99 1.0 0.95 0.94 0.95 0.92 0.93 0.95 0.94 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.12 0.29 0.12 0.41 0.26 0.53 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 2 0 0 0
Bud 3 1 1 0 0 1 10 2 12 10 12 18 0 0 0 0 0 0 0 0 0 1 3 2
Bud Neck 0 0 1 0 0 2 1 1 2 3 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 4 11 6 9 6 5 6 6 3 10 4 2 7 10 8 6 0 0 0 0 0 0
Cytoplasm 134 242 281 310 412 296 452 540 470 574 279 442 250 314 372 123 116 102 136 230 263 208 350 324
Endoplasmic Reticulum 2 1 1 0 0 0 0 3 1 1 0 2 0 1 1 4 5 5 1 2 1 1 1 4
Endosome 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 2 0 0 0 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 2 1
Mitochondria 10 2 0 5 12 37 156 75 259 180 223 160 0 0 1 0 1 2 1 2 5 2 3 2
Nucleus 0 0 0 2 0 2 5 7 3 3 2 1 2 0 0 5 1 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 2 1 0 2 0 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 1 1 5 1 3 0 0 0 0 0 0 1 0 0 0 0 2
Vac/Vac Membrane 0 1 1 2 3 6 8 4 7 9 9 10 0 1 0 3 2 3 2 3 0 2 1 1
Unique Cell Count 140 246 281 316 419 317 530 601 626 701 418 547 251 316 373 130 124 107 148 249 277 222 369 344
Labelled Cell Count 149 252 289 331 434 354 641 639 763 795 532 654 257 319 381 145 134 119 148 249 277 222 369 344


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 6.6 6.3 5.9 5.9 4.5 4.1 4.7 3.9 4.3 3.7 4.3 6.4 6.8 6.7 8.8 8.6 8.3 6.4 6.4 7.0
Std Deviation (1e-4) 0.6 0.9 1.0 1.6 1.9 0.9 1.0 1.0 0.9 1.1 0.7 0.9 0.9 1.5 1.4 1.2 1.3 1.4 1.6 1.0 1.4
Intensity Change (Log2) – – – -0.09 -0.09 -0.49 -0.6 -0.41 -0.71 -0.57 -0.78 -0.56 0.02 0.1 0.09 0.48 0.44 0.4 0.02 0.02 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -2.2 -4.4 -6.8 -5.5 -9.2 -7.6 -10.8 -7.9 -1.1 -1.3 -0.9 -3.9 -4.3 -3.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6283 2.3255 2.0548 1.9509 1.7251 2.1141 0.9673 2.242 1.7452 1.8252 1.6007 1.8322 0.8131 1.4294 1.2539 1.4775 1.2381 1.2214
Actin 0.0126 0.0009 0.0062 0.0089 0.0429 0.0072 0.0081 0.0004 0.0096 0.0003 0.0001 0.0002 0.0307 0.0003 0.0188 0.0239 0.0027 0.0072
Bud 0.0005 0.0003 0.0004 0.0008 0.0013 0.0001 0.0003 0.0161 0.0013 0.0002 0.0001 0 0.0057 0.0002 0.0002 0.009 0.0005 0.0063
Bud Neck 0.0018 0.0002 0.0011 0.0006 0.0015 0.0004 0.0007 0.0001 0.0006 0.0001 0.0003 0.0004 0.001 0.0001 0.0006 0.0092 0.0003 0.0008
Bud Periphery 0.0006 0.0002 0.0006 0.0011 0.0043 0.0001 0.0004 0.0038 0.0012 0.0001 0.0001 0 0.0108 0.0001 0.0001 0.0138 0.0004 0.0026
Bud Site 0.0021 0.002 0.0018 0.0095 0.0015 0.0001 0.001 0.0041 0.0034 0.0002 0.0001 0 0.0069 0.0004 0.0039 0.0032 0.0017 0.0015
Cell Periphery 0.0002 0.0002 0.0001 0.0003 0.0003 0 0.0001 0.0002 0.0001 0.0001 0 0 0.0007 0.0001 0.0001 0.0006 0.0001 0.0001
Cytoplasm 0.5303 0.7276 0.5711 0.6455 0.5194 0.618 0.5679 0.7804 0.736 0.7468 0.7531 0.8585 0.6381 0.7918 0.7148 0.6882 0.6602 0.7805
Cytoplasmic Foci 0.03 0.009 0.0133 0.0253 0.0245 0.0177 0.0182 0.0053 0.0067 0.0104 0.0303 0.0066 0.0141 0.006 0.0181 0.0377 0.0161 0.0092
Eisosomes 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0.0008 0 0.0001 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0041 0.0021 0.0024 0.0032 0.0046 0.0016 0.004 0.0024 0.0029 0.0052 0.0009 0.0013 0.0072 0.0071 0.0028 0.0027 0.0052 0.002
Endosome 0.016 0.0043 0.0094 0.0175 0.02 0.0073 0.0148 0.0033 0.0038 0.0066 0.0089 0.0023 0.0218 0.0016 0.0109 0.0254 0.051 0.0085
Golgi 0.0036 0.0004 0.0013 0.0051 0.0121 0.0023 0.0025 0.0001 0.0004 0.0003 0.0003 0.0001 0.0112 0.0003 0.0042 0.0192 0.0096 0.0038
Lipid Particles 0.0056 0.0001 0.001 0.0072 0.0177 0.0016 0.0058 0.0001 0.0002 0.0002 0.0001 0.0001 0.0049 0.0001 0.019 0.0047 0.0047 0.0015
Mitochondria 0.0025 0.0003 0.0013 0.01 0.0106 0.0036 0.0009 0.0005 0.0008 0.0003 0.0004 0.0001 0.0245 0.0003 0.0009 0.0244 0.0026 0.0039
None 0.3683 0.2482 0.3762 0.2337 0.2815 0.3271 0.3558 0.1781 0.2225 0.2238 0.1981 0.1287 0.2082 0.1888 0.1915 0.1005 0.1962 0.1574
Nuclear Periphery 0.0025 0.0006 0.0018 0.0026 0.0014 0.0016 0.0032 0.0011 0.0017 0.0012 0.0008 0.0002 0.0023 0.0006 0.0012 0.0013 0.0074 0.0011
Nucleolus 0.0007 0.0001 0.001 0.0007 0.0003 0.0001 0.0012 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0008 0.0022 0.0011
Nucleus 0.0016 0.0015 0.002 0.003 0.0011 0.0011 0.002 0.0016 0.0028 0.0017 0.0018 0.0007 0.0009 0.0006 0.0007 0.0025 0.0025 0.0077
Peroxisomes 0.0111 0.0001 0.0051 0.0199 0.0522 0.0063 0.0034 0.0001 0.0005 0.0001 0.0003 0 0.0052 0.0002 0.0069 0.0208 0.0016 0.0014
Punctate Nuclear 0.0036 0.0005 0.002 0.0021 0.0015 0.0029 0.0075 0.0005 0.0036 0.0006 0.0021 0.0003 0.0008 0.0007 0.004 0.003 0.0303 0.002
Vacuole 0.0017 0.0014 0.0014 0.0021 0.0008 0.0006 0.0015 0.0014 0.0013 0.0015 0.0019 0.0004 0.0028 0.0007 0.0009 0.0065 0.0034 0.0011
Vacuole Periphery 0.0004 0.0002 0.0003 0.0007 0.0005 0.0003 0.0004 0.0002 0.0002 0.0003 0.0003 0.0001 0.0009 0.0001 0.0002 0.0022 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.993 8.0411 11.2707 14.0526 15.5501 2.9967 12.6472 13.4138 14.7994 14.5062
Translational Efficiency 0.8051 1.8751 1.0741 0.9574 0.8323 3.9451 1.2281 1.3562 0.851 0.8197

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dus4

Dus4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dus4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available