Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.07
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.35 0.17 0.5 0.44 0.66 – 0.54 0.47 0.41 0.39 0.39 0.37 0.4 0.45 0.29 0.33 0.45 0.1 0.1 0.11 0.16 0.12 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.08 0 – 0 0 0 0 0 0 0 0 0.05 0 0.14 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 – 0.15 0.25 0.24 0.31 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0 0.11 0 0 0 – 0.09 0 0.07 0.06 0.09 0 0 0 0 0 0 0.61 0.64 0.48 0.45 0.53 0.52
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.83 0.78 0.53 0.53 0.34 – 0.31 0.32 0.29 0.26 0.19 0.71 0.65 0.65 0.73 0.68 0.55 0.11 0.15 0.17 0.23 0.19 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 1 0 2 0 0 0 1 0 0 2 0 1 0 0 0 1 0
Bud 0 0 1 0 0 0 – 1 1 2 0 2 0 0 0 0 0 0 13 1 3 5 10 14
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 – 0 1 1 1 6 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 68 25 9 15 28 79 – 99 96 107 77 123 98 137 204 42 31 10 27 8 10 24 25 22
Endoplasmic Reticulum 2 0 0 1 1 3 – 6 4 11 16 14 6 4 3 5 2 0 2 0 1 3 2 1
Endosome 0 0 0 0 0 0 – 0 0 3 0 1 0 0 0 2 1 0 5 0 2 5 3 4
Golgi 1 3 0 1 5 1 – 0 0 1 0 0 5 2 10 8 4 3 0 0 0 0 3 1
Mitochondria 1 0 0 0 0 9 – 28 52 62 61 114 0 0 1 3 1 0 1 0 0 1 0 0
Nucleus 23 3 6 1 0 3 – 17 6 17 12 28 7 4 3 3 2 0 170 57 46 67 114 107
Nuclear Periphery 0 0 0 0 0 1 – 1 4 2 1 7 0 0 0 0 0 0 8 1 7 2 2 2
Nucleolus 0 0 0 0 0 0 – 0 0 1 0 1 0 1 0 2 1 0 10 2 2 2 4 2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 0 1 2 3 3
Vac/Vac Membrane 219 60 42 16 34 40 – 57 65 76 52 58 187 223 295 108 64 12 32 13 16 34 40 44
Unique Cell Count 263 72 54 30 64 119 183 205 258 197 313 264 342 454 147 94 22 282 90 96 151 217 208
Labelled Cell Count 315 91 58 34 69 136 210 229 285 220 354 303 372 519 173 108 25 282 90 96 151 217 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.4 16.2 9.5 13.3 14.1 12.4 – 10.0 9.2 9.3 8.7 8.3 16.5 16.8 16.1 19.4 17.5 17.5 13.9 14.6 15.3
Std Deviation (1e-4) 2.4 3.1 2.1 1.8 3.0 2.3 – 2.2 2.3 2.3 2.0 1.9 3.0 5.1 3.6 5.2 5.1 6.7 3.1 3.1 3.9
Intensity Change (Log2) – – – 0.48 0.57 0.38 – 0.07 -0.05 -0.03 -0.14 -0.2 0.79 0.82 0.75 1.02 0.88 0.88 0.54 0.62 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 3.2 6.1 – 4.9 4.0 3.4 3.1 3.2 2.9 3.3 4.0 1.7 2.2 2.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 3.1 4.1 4.0 4.7 5.3 0 0 0 0 0 0
Nucleus 0 0 0 – -0.4 0 0 0 -0.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.3 -2.8 -5.4 – -6.1 -6.1 -6.7 -6.9 -9.0 -1.0 -1.8 -1.9 -0.6 -1.3 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.5286 14.7601 13.7862 13.1479 12.4959 13.7195 12.7271 15.9218 14.2263 12.2768 13.9781 14.2158 14.3728 16.5347 16.7202 15.3331 15.51 16.538
Actin 0.0131 0.0001 0.0001 0 0.0002 0.0002 0.0361 0 0.002 0.0041 0.0001 0.0109 0.0211 0.0004 0.0022 0.0183 0.0003 0.0001
Bud 0.0003 0 0 0 0 0.0001 0.0005 0 0.0001 0.0001 0 0.0002 0.0004 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0004 0.0002 0.0001 0 0.0005 0.0007 0.0008 0.0001 0.0001 0.0002 0.0005 0.001 0.0007 0.0003 0.0003 0.0003 0.0007 0.0011
Bud Periphery 0.0004 0 0 0 0 0.0001 0.0005 0 0.0002 0.0002 0 0.0002 0.0008 0.0001 0.0001 0.0002 0 0
Bud Site 0.0026 0.0001 0 0 0 0 0.0043 0 0.0007 0.0004 0 0.0002 0.0005 0.0006 0.0035 0.0003 0.0001 0
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0 0 0 0
Cytoplasm 0.0119 0.0399 0.0062 0.0019 0.0044 0.007 0.0131 0.005 0.0053 0.0055 0.0332 0.007 0.0138 0.0137 0.0051 0.0185 0.0359 0.0046
Cytoplasmic Foci 0.0122 0 0 0 0 0 0.0213 0 0.007 0.0052 0.0001 0.0022 0.0069 0.002 0.0007 0.0028 0.0005 0
Eisosomes 0.0002 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0.0001 0.0003 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0013 0.0003 0.0002 0 0.0006 0.0005 0.003 0.0001 0.0002 0.001 0.001 0.002 0.0084 0.0016 0.0006 0.0008 0.0059 0.0005
Endosome 0.0078 0.0002 0 0 0.0002 0.0001 0.0162 0 0.004 0.034 0.0011 0.0177 0.0129 0.0027 0.0026 0.0067 0.003 0.0003
Golgi 0.0037 0 0 0 0 0 0.0083 0 0.0016 0.0096 0 0.005 0.003 0.0004 0.0005 0.0054 0 0
Lipid Particles 0.0104 0 0 0 0 0 0.0148 0 0.0042 0.036 0 0.0033 0.0087 0.0039 0.0005 0.006 0 0
Mitochondria 0.0064 0.0002 0.0002 0.0002 0.0002 0.0003 0.0036 0.0001 0.0126 0.0307 0.0002 0.002 0.0127 0.0013 0.0004 0.0014 0.0003 0.0001
None 0.0008 0 0 0 0 0 0.0024 0 0.0003 0.0002 0.0001 0.0004 0.0033 0.001 0.0007 0.0004 0.0004 0
Nuclear Periphery 0.0908 0.0528 0.0621 0.0221 0.1151 0.0572 0.0708 0.0411 0.044 0.0342 0.0473 0.0753 0.1009 0.0505 0.0652 0.0615 0.1132 0.0514
Nucleolus 0.0017 0.0014 0.0006 0.0007 0.0017 0.0033 0.0014 0.0008 0.0008 0.0006 0.0017 0.0026 0.0034 0.0015 0.001 0.0005 0.0019 0.0016
Nucleus 0.8223 0.9031 0.9294 0.9746 0.8751 0.9289 0.7739 0.952 0.9061 0.8296 0.9111 0.8615 0.786 0.9152 0.9121 0.8711 0.8319 0.9386
Peroxisomes 0.0082 0 0 0 0 0 0.0105 0 0.0073 0.003 0 0.0008 0.0022 0.0004 0.0002 0.0023 0 0
Punctate Nuclear 0.0018 0.0006 0.0006 0.0002 0.0008 0.0008 0.0142 0.0004 0.0027 0.0012 0.0012 0.0043 0.0039 0.0023 0.0032 0.0023 0.0036 0.0009
Vacuole 0.0021 0.0006 0.0002 0.0001 0.0005 0.0004 0.002 0.0002 0.0004 0.0021 0.0015 0.0019 0.0057 0.001 0.0004 0.0004 0.0015 0.0004
Vacuole Periphery 0.0015 0.0004 0.0002 0.0001 0.0007 0.0004 0.002 0.0001 0.0003 0.0021 0.0007 0.0015 0.0042 0.0008 0.0003 0.0005 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.994 92.1455 102.564 93.4485 100.234 77.2658 77.2873 94.3234 143.6052 83.1986
Translational Efficiency 2.6905 2.0413 1.8286 2.8585 1.8992 3.4062 2.7954 2.2272 1.6101 2.1669

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (32%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-6

Rpn13

Rpn13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available